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A Label-Free Fluorescence Polarization for Protein Detection Based on Albumin Nanoparticles 基于白蛋白纳米颗粒的无标记荧光偏振蛋白检测
3区 工程技术
Chemosensors Pub Date : 2025-11-12 DOI: 10.3390/chemosensors13110396
Yunshu Wang, Ruixin An, Fengwei Liu, Zhongyun Dong, Yang Sheng, Siyu Liu
{"title":"A Label-Free Fluorescence Polarization for Protein Detection Based on Albumin Nanoparticles","authors":"Yunshu Wang, Ruixin An, Fengwei Liu, Zhongyun Dong, Yang Sheng, Siyu Liu","doi":"10.3390/chemosensors13110396","DOIUrl":"https://doi.org/10.3390/chemosensors13110396","url":null,"abstract":"Human serum albumin (HSA), an endogenous protein, was employed in the synthesis of nanoparticles. The treatment of an HSA solution with ethanol and glutaraldehyde resulted in the formation of human serum albumin nanoparticles (HSA NPs), which exhibited a weak fluorescence emission peak at 515 nm upon excitation at 360 nm. Importantly, these synthesized HSA NPs displayed a pronounced fluorescence polarization (FP) signal under identical excitation and emission conditions. Furthermore, incubation of the HSA NPs with specific DNA aptamers targeting lysozyme and thrombin led to a significant decrease in the FP values. This reduction in FP was effectively reversed upon the addition of lysozyme and thrombin. Based on these observations, a label-free fluorescence polarization-based detection platform for lysozyme and thrombin was developed utilizing HSA NPs and a DNA aptamer system.","PeriodicalId":10057,"journal":{"name":"Chemosensors","volume":"13 11","pages":"396-396"},"PeriodicalIF":0.0,"publicationDate":"2025-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2227-9040/13/11/396/pdf?version=1762942371","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147332508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Room Temperature Sub-ppm NO2 Gas Sensor Based on Ag/SnS2 Heterojunction Driven by Visible Light 可见光驱动Ag/SnS2异质结室温亚ppm NO2气体传感器
3区 工程技术
Chemosensors Pub Date : 2025-10-10 DOI: 10.3390/chemosensors13100368
Ding Gu, Jun Dong, Wei Liu, Xiaogan Li
{"title":"Room Temperature Sub-ppm NO2 Gas Sensor Based on Ag/SnS2 Heterojunction Driven by Visible Light","authors":"Ding Gu, Jun Dong, Wei Liu, Xiaogan Li","doi":"10.3390/chemosensors13100368","DOIUrl":"https://doi.org/10.3390/chemosensors13100368","url":null,"abstract":"As industrial waste gas, nitrogen dioxide (NO2) is a serious hazard to air pollution and human health, and there is a pressing demand for developing high-performance NO2 gas sensors. Tin disulfide (SnS2), a representative two-dimensional metal sulfide characterized by a significant specific surface area, a suitable electron band gap, and an easily tunable layered structure, shows a broad application prospect in gas sensing applications. Nevertheless, SnS2-based gas sensors suffer from poor sensitivity, which seriously hinders their application in room temperature gas sensing. In this study, Ag/SnS2 heterojunction nanomaterials were synthesized by an in situ reduction approach. The findings reveals that the gas-sensitive response of the Ag/SnS2 nanocomposites at room temperature under visible light irradiation can achieve 10.5 to 1 ppm NO2, with a detection limit as low as 200 ppb, which realizes the room-temperature detection of Sub-ppm NO2. Meanwhile, the sensor exhibits good selectivity, reproducibility (cyclic stability > 95%). The improved gas sensitivity of the Ag/SnS2 sensor can be due to the synergistic effect of the carrier separation at the Ag/SnS2 Schottky junction and the localized surface plasmon resonance (LSPR) of Ag nanoparticles. The LSPR effect significantly enhances light absorption and surface-active site density, facilitating trace NO2 detection at room temperature. This study provides the foundation for the subsequent development of room temperature layered metal sulfide gas sensors.","PeriodicalId":10057,"journal":{"name":"Chemosensors","volume":"13 10","pages":"368-368"},"PeriodicalIF":0.0,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2227-9040/13/10/368/pdf?version=1760106597","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147909673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Profiling Hydrogen-Bond Conductance via Fixed-Gap Tunnelling Sensors in Physiological Solution 生理溶液中固定间隙隧道传感器的氢键电导分析
3区 工程技术
Chemosensors Pub Date : 2025-10-02 DOI: 10.3390/chemosensors13100360
Biao‐Feng Zeng, Canyu Yan, Ye Tian, Yuxin Yang, Long Yi, Shiyang Fu, Xu Liu, Cuifang Kuang, Longhua Tang
{"title":"Profiling Hydrogen-Bond Conductance via Fixed-Gap Tunnelling Sensors in Physiological Solution","authors":"Biao‐Feng Zeng, Canyu Yan, Ye Tian, Yuxin Yang, Long Yi, Shiyang Fu, Xu Liu, Cuifang Kuang, Longhua Tang","doi":"10.3390/chemosensors13100360","DOIUrl":"https://doi.org/10.3390/chemosensors13100360","url":null,"abstract":"Hydrogen bonding, a prevalent molecular interaction in nature, is crucial in biological and chemical processes. The emergence of single-molecule techniques has enhanced our microscopic understanding of hydrogen bonding. However, it is still challenging to track the dynamic behaviour of hydrogen bonding in solution, particularly under physiological conditions where interactions are significantly weakened. Here, we present a nanoscale-confined, functionalised quantum mechanical tunnelling (QMT) probe that enables continuous monitoring of electrical fingerprints of single-molecule hydrogen bonding interactions for over tens of minutes in diverse solvents, including polar physiological solutions, which reveal reproducible multi-level conductance distributions. Moreover, the functionalised QMT probes have successfully discriminated between L(+)- and D(−)-tartaric acid enantiomers by resolving the conductance difference. This work uncovers dynamic single-molecule hydrogen bonding processes within confined nanoscale spaces under physiological conditions, establishing a new paradigm for probing molecular hydrogen-bonding networks in supramolecular chemistry and biology.","PeriodicalId":10057,"journal":{"name":"Chemosensors","volume":"13 10","pages":"360-360"},"PeriodicalIF":0.0,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147919515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
An Electrochemical Aptamer Sensor with ZIF-8 Loaded CuNPs Composites for Aflatoxin B1 Determination ZIF-8负载CuNPs复合材料的电化学适体传感器测定黄曲霉毒素B1
3区 工程技术
Chemosensors Pub Date : 2025-09-06 DOI: 10.3390/chemosensors13090342
Juncheng Chen, Caizhang Wu, Zhike Zhao, Ruihao Xue
{"title":"An Electrochemical Aptamer Sensor with ZIF-8 Loaded CuNPs Composites for Aflatoxin B1 Determination","authors":"Juncheng Chen, Caizhang Wu, Zhike Zhao, Ruihao Xue","doi":"10.3390/chemosensors13090342","DOIUrl":"https://doi.org/10.3390/chemosensors13090342","url":null,"abstract":"An electrochemical aptamer sensor for the sensitive detection of aflatoxin B1 (AFB1) in corn samples was developed using nanocomposites loaded with copper nanoparticles (CuNPs) on zeolitic imidazolate framework-8 (ZIF-8), which were modified on a glassy carbon electrode (GCE). The CuNPs@ZIF-8 nanocomposites served as modifying materials for electrodes, exhibiting a high specific surface area and excellent compatibility with aptamers, thereby enhancing the electron transfer rate and increasing the aptamer loading and immobilization efficiency. The electrochemical properties before and after modification were investigated and validated using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) techniques, while the sensor’s performance was analyzed through quantitative detection via differential pulse voltammetry (DPV). Furthermore, various conditions, including the volume of ZIF-8 dispersion, electrodeposition time of copper nanoparticles, aptamer concentration, and AFB1 incubation time, were optimized. The results indicated that the sensor exhibited a wide linear range (10.0 to 1.0 × 106 pg/mL), with a lower limit of detection (LOD) of 1.13 pg/mL under optimized conditions, outperforming other reported aptamer sensors. The spiked recoveries in corn samples ranged from 96.663% to 105.72%. In conclusion, this sensor presents a novel solution for low-cost and high-sensitivity detection of AFB1.","PeriodicalId":10057,"journal":{"name":"Chemosensors","volume":"13 9","pages":"342-342"},"PeriodicalIF":0.0,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2227-9040/13/9/342/pdf?version=1757146097","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147908388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
High-Intensity In Situ Fluorescence Imaging of MicroRNA in Cells Based on Y-Shaped Cascade Assembly 基于y形级联组装的细胞中MicroRNA的高强度原位荧光成像
3区 工程技术
Chemosensors Pub Date : 2025-09-06 DOI: 10.3390/chemosensors13090343
Yan Liu, Xu Fan, Xinying Zhou, Zhiqi Zhang, Qi Yang, Rongjie Yang, Yingxue Li, Anran Zheng, Lianqun Zhou, Wei Zhang, Jinze Li
{"title":"High-Intensity In Situ Fluorescence Imaging of MicroRNA in Cells Based on Y-Shaped Cascade Assembly","authors":"Yan Liu, Xu Fan, Xinying Zhou, Zhiqi Zhang, Qi Yang, Rongjie Yang, Yingxue Li, Anran Zheng, Lianqun Zhou, Wei Zhang, Jinze Li","doi":"10.3390/chemosensors13090343","DOIUrl":"https://doi.org/10.3390/chemosensors13090343","url":null,"abstract":"MicroRNAs are closely associated with various physiological and pathological processes, making their in situ fluorescence imaging crucial for functional studies and disease diagnosis. Current methods for the in situ fluorescence imaging of microRNA predominantly rely on linear signal amplification, resulting in relatively weak imaging signals. This study introduces a Y-shaped cascade assembly (YCA) method for high-brightness microRNA imaging in cells. Triggered by target microRNA, catalytic hairpin assembly forms double-stranded DNA (H). Through annealing and hybridization, a Y-shaped structure (P) is created. These components assemble into DNA nanofluorescent particles with multiple FAM fluorophores, significantly amplifying fluorescence signals. Optimization experiments revealed that a 1:1 ratio of P to H and an assembly time of 60 min yielded the best results. Under these optimal conditions, the resulting fluorescent nanoparticles exhibited diameters of 664.133 nm, as observed by DLS. In Huh7 liver cancer cells, YCA generated DNA nanoparticles with a fluorescence intensity increase of 117.77%, triggered by target microRNA-21, producing high-intensity fluorescence images and enabling qualitative detection of microRNA-21. The YCA in situ imaging method offers excellent imaging quality and high efficiency, providing a robust and reliable analytical tool for the diagnosis and monitoring of microRNA-related diseases.","PeriodicalId":10057,"journal":{"name":"Chemosensors","volume":"13 9","pages":"343-343"},"PeriodicalIF":0.0,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2227-9040/13/9/343/pdf?version=1757313664","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147331941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A Ratiometric Fluorescent Probe Based on CDs-Functionalized UiO-66 for Efficient Detection of Uric Acid 基于cd功能化UiO-66的比例荧光探针高效检测尿酸
3区 工程技术
Chemosensors Pub Date : 2025-09-05 DOI: 10.3390/chemosensors13090340
Hongmei Gao, Yourong Zhao, Yuhong Xie, Yi-Ying Wang, Jie Che, Daojiang Gao, Zhanglei Ning
{"title":"A Ratiometric Fluorescent Probe Based on CDs-Functionalized UiO-66 for Efficient Detection of Uric Acid","authors":"Hongmei Gao, Yourong Zhao, Yuhong Xie, Yi-Ying Wang, Jie Che, Daojiang Gao, Zhanglei Ning","doi":"10.3390/chemosensors13090340","DOIUrl":"https://doi.org/10.3390/chemosensors13090340","url":null,"abstract":"In this study, a novel carbon quantum dots-functionalized UiO-66 composite was successfully prepared via the post-synthetic modification method and further developed into a ratiometric fluorescent probe for detecting uric acid. The composite demonstrates excellent structural and luminescent stability under challenging environmental conditions. As a ratiometric fluorescent probe, its recognition principle relies on the ratio of response signals from two different fluorescent emission centers in the composite. In the presence of uric acid, the fluorescence emission intensity at 430 nm from CDs did not change significantly. However, the fluorescence intensity at 545 nm from Tb3+ ions decreased remarkably. This material was evaluated for its capacity to sense urinary components and was shown to specifically recognize uric acid over a wide concentration range (0~5 × 10−3 M). Moreover, it exhibited strong resistance to interference and high sensitivity in uric acid detection. The detection limit (LOD) was determined to be 0.102 μM through quantitative analysis. The sensing mechanism was validated through spectral overlap and fluorescence lifetime analysis, which can be attributed to the fluorescence resonance energy transfer (FRET) process. This ratiometric fluorescent probe provides an efficient and reliable strategy for detecting the biomarker uric acid.","PeriodicalId":10057,"journal":{"name":"Chemosensors","volume":"13 9","pages":"340-340"},"PeriodicalIF":0.0,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2227-9040/13/9/340/pdf?version=1757078953","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147915668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
A Carbon Black-Based Non-Enzymatic Electrochemical Sensor for the Detection of Sunset Yellow in Beverages 基于炭黑的非酶电化学传感器检测饮料中日落黄
3区 工程技术
Chemosensors Pub Date : 2025-09-02 DOI: 10.3390/chemosensors13090330
Zihui Li, Wenxue Chen, Qiongya Wan, Haoliang Li, Xuefeng Wang, Pengcheng Xu, Yuan Zhang, Yongheng Zhu, Xinxin Li
{"title":"A Carbon Black-Based Non-Enzymatic Electrochemical Sensor for the Detection of Sunset Yellow in Beverages","authors":"Zihui Li, Wenxue Chen, Qiongya Wan, Haoliang Li, Xuefeng Wang, Pengcheng Xu, Yuan Zhang, Yongheng Zhu, Xinxin Li","doi":"10.3390/chemosensors13090330","DOIUrl":"https://doi.org/10.3390/chemosensors13090330","url":null,"abstract":"This study presents a highly sensitive non-enzymatic electrochemical sensor for detecting Sunset Yellow, a common food additive in beverages, based on palladium-cerium oxide composite decorated carbon black (CB). The sensing material was prepared by depositing palladium nanoparticles onto cerium oxide nanocubes, followed by the uniform dispersion of CB through sonication in a water bath. The strong metal–support interaction between palladium and cerium oxide significantly enhances catalytic activity, while the CB ensures excellent conductivity and structural support for the catalyst. Under optimized conditions, the sensor exhibits a linear response to Sunset Yellow concentrations in the range from 1 to 100 nM, with a limit of detection (LOD) of 0.056 nM. Additionally, the sensor demonstrates remarkable selectivity and stability. Practical application in real orange juice samples yielded recoveries from 99.11% and 101.34%, confirming its reliability for real-world beverage analysis.","PeriodicalId":10057,"journal":{"name":"Chemosensors","volume":"13 9","pages":"330-330"},"PeriodicalIF":0.0,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2227-9040/13/9/330/pdf?version=1756811350","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147903070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Construction of a Photonic Crystal (PC) Film Sensing Platform Based on Calcium Alginate Hydrogel for the Trichlorfon Detection 海藻酸钙水凝胶光子晶体(PC)传感平台的构建及其对敌百虫的检测
3区 工程技术
Chemosensors Pub Date : 2025-08-13 DOI: 10.3390/chemosensors13080306
Junjie Ren, Xia Li, Zhongxing Wang, Li Yu
{"title":"Construction of a Photonic Crystal (PC) Film Sensing Platform Based on Calcium Alginate Hydrogel for the Trichlorfon Detection","authors":"Junjie Ren, Xia Li, Zhongxing Wang, Li Yu","doi":"10.3390/chemosensors13080306","DOIUrl":"https://doi.org/10.3390/chemosensors13080306","url":null,"abstract":"Trichlorfon, an organophosphorus pesticide widely used in agriculture and other fields, poses a severe risk to both food safety and human health. We developed a photonic crystal film sensing platform for detecting trichlorfon, a hazardous organophosphorus pesticide. The method exploits trichlorfon’s inhibition of acetylcholinesterase (AChE). Normally, AChE catalyzes acetylcholine hydrolysis to produce acetic acid, which decomposes CaCO3 to release Ca2+. This triggers calcium alginate hydrogel formation, increasing solution viscosity and trapping water. When trichlorfon inhibits AChE, hydrogel formation fails, leaving the solution in a low-viscosity sol state with abundant free water. Immersing the film in trichlorfon-containing sodium alginate solutions causes water absorption and film swelling due to free water. Higher trichlorfon concentrations reduce hydrogel formation, increase free water, and amplify film swelling, resulting in proportionally higher reflectivity. The platform demonstrates a wide linear range (1–250 ng/mL) and a low detection limit (0.4 ng/mL) for trichlorfon. Successful analysis of real samples confirms its practicality for residue detection. This label-free thin-film sensor shows significant potential for monitoring trichlorfon and other organophosphorus pesticides.","PeriodicalId":10057,"journal":{"name":"Chemosensors","volume":"13 8","pages":"306-306"},"PeriodicalIF":0.0,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147894935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
A pH-Responsive Liquid Crystal-Based Sensing Platform for the Detection of Biothiols 一种ph响应液晶检测生物硫醇的传感平台
3区 工程技术
Chemosensors Pub Date : 2025-08-06 DOI: 10.3390/chemosensors13080291
Xianghao Meng, Ronghua Zhang, Xinfeng Dong, Zhongxing Wang, Li Yu
{"title":"A pH-Responsive Liquid Crystal-Based Sensing Platform for the Detection of Biothiols","authors":"Xianghao Meng, Ronghua Zhang, Xinfeng Dong, Zhongxing Wang, Li Yu","doi":"10.3390/chemosensors13080291","DOIUrl":"https://doi.org/10.3390/chemosensors13080291","url":null,"abstract":"Biothiols, including cysteine (Cys), homocysteine (Hcy), and glutathione (GSH), are crucial for physiological regulation and their imbalance poses severe health risks. Herein, we developed a pH-responsive liquid crystal (LC)-based sensing platform for detection of biothiols by doping 4-n-pentylbiphenyl-4-carboxylic acid (PBA) into 4-n-pentyl-4-cyanobiphenyl (5CB). Urease catalyzed urea hydrolysis to produce OH−, triggering the deprotonation of PBA, thereby inducing a vertical alignment of LC molecules at the interface corresponding to dark optical appearances. Heavy metal ions (e.g., Hg2+) could inhibit urease activity, under which condition LC presents bright optical images and LC molecules maintain a state of tilted arrangement. However, biothiols competitively bind to Hg2+, the activity of urease is maintained which enables the occurrence of urea hydrolysis. This case triggers LC molecules to align in a vertical orientation, resulting in bright optical images. This pH-driven reorientation of LCs provides a visual readout (bright-to-dark transition) correlated with biothiol concentration. The detection limits of Cys/Hcy and GSH for the PBA-doped LC platform are 0.1 μM and 0.5 μM, respectively. Overall, this study provides a simple, label-free and low-cost strategy that has a broad application prospect for the detection of biothiols.","PeriodicalId":10057,"journal":{"name":"Chemosensors","volume":"13 8","pages":"291-291"},"PeriodicalIF":0.0,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2227-9040/13/8/291/pdf?version=1754480069","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147878837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
A Long-Wavelength Fluorescent Probe for Efficient Dual-Color Imaging of Boronic-Acid-Containing Agents in Living Cells 一种用于活细胞中含硼酸剂高效双色成像的长波长荧光探针
3区 工程技术
Chemosensors Pub Date : 2025-08-04 DOI: 10.3390/chemosensors13080283
Shinya Takada, H. Du, Naoya Kondo, A. Miyazaki, Fumiko Hara, Shizuyo Horiyama, Takashi Temma, Masayori Hagimori
{"title":"A Long-Wavelength Fluorescent Probe for Efficient Dual-Color Imaging of Boronic-Acid-Containing Agents in Living Cells","authors":"Shinya Takada, H. Du, Naoya Kondo, A. Miyazaki, Fumiko Hara, Shizuyo Horiyama, Takashi Temma, Masayori Hagimori","doi":"10.3390/chemosensors13080283","DOIUrl":"https://doi.org/10.3390/chemosensors13080283","url":null,"abstract":"In boron neutron capture therapy (BNCT), the intracellular localization and concentration of boron-10 atoms significantly influence therapeutic efficacy. Although various boronic-acid-targeted fluorescent probes have been developed to evaluate BNCT agents, most of these probes emit at short wavelengths and are, therefore, incompatible with common nuclear-staining reagents such as Hoechst 33342 and 4′,6-diamidino-2-phenylindole (DAPI). While our previously reported probe, BS-631, emitted fluorescence above 500 nm, it exhibited limitations in terms of reaction rate and fluorescence intensity. To address these issues, we developed a boronic-acid-targeted fluorescent probe with a longer emission wavelength, rapid reactivity, and strong fluorescence intensity. Herein, we designed and synthesized BTTQ, a probe based on a 2-(2-hydroxyphenyl)benzothiazole core structure. BTTQ exhibited immediate fluorescence upon reaction with 4-borono-L-phenylalanine (BPA), with an emission wavelength of 567 nm and a sufficiently high fluorescence quantum yield for detection. BTTQ quantitatively detected BPA with high sensitivity (quantification limit of 10.27 µM), suitable for evaluating BNCT agents. In addition, BTTQ exhibited selective fluorescence for BPA over metal cations. Importantly, BTTQ enabled fluorescence microscopic imaging of intracellular BPA distribution in living cells co-stained with Hoechst 33342. These results suggest that BTTQ is a promising fluorescent probe for the evaluation of future BNCT agents.","PeriodicalId":10057,"journal":{"name":"Chemosensors","volume":"13 8","pages":"283-283"},"PeriodicalIF":0.0,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2227-9040/13/8/283/pdf?version=1754289417","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147913018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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