Rapid and Sensitive On-Site Detection of Fipronil in Foods Using Evanescent Wave Fluorescent Immunosensor

IF 3.7 3区工程技术 Q2 CHEMISTRY, ANALYTICAL

Chemosensors Pub Date : 2023-12-17 DOI:10.3390/chemosensors11120578

Yujing Li, Wenjuan Xu, Jingjing Liu, E. Zhang, Hejie Li, Yan Zhang, Jing Zhang, Chunsheng Li, Xiaoguang Zhang

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引用次数: 0

Abstract

Fipronil (FIP), a broad-spectrum phenylpyrazole insecticide, is highly toxic and threatens human health and ecological balance. Developing convenient, rapid, portable analytical technology for on-site and high-frequency testing of FIP is essential to reduce its damage. Herein, a monoclonal antibody (Clone F-3F6) against FIP, with high affinity and specificity, was produced using a novel immunogen, FIP-BSA, which was simply and directly synthesized by conjugating FIP with bovine serum albumin (BSA). Among the previously reported antibodies, F-3F6 acts more specifically against FIP. The FIP metabolites fipronil desulfinyl, fipronil sulfide, and fipronil sulfone showed lower cross-reactivity, and other pesticides were not recognized. To achieve high-frequency and on-site measurements of FIP, an evanescent wave fluorescence biosensor was built by integrating evanescent wave fluorescence technology, a functionalized fiber bioprobe, and a fluorescence-labeled F-3F6 antibody. The detection limit of FIP was 0.032 μg/L. The detection results of real milk and water samples showed that all the coefficients of variation were less than 10%, and the recovery ranged from 90 to 120%. The high reusability and stability of functionalized fiber bioprobe enables the accurate, cost-effective, high-frequency, and facile quantitative detection of FIP. This highly specific and reliable evanescent wave fluorescence biosensor will be well suited to the sensitive and high-frequency on-site analysis of only FIP in food.

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利用 Evanescent Wave 荧光免疫传感器快速灵敏地现场检测食品中的氟虫腈

氟虫腈（FIP）是一种广谱苯基吡唑类杀虫剂，具有剧毒，威胁人类健康和生态平衡。开发方便、快速、便携的分析技术，对氟虫腈进行现场高频检测，对减少其危害至关重要。本文利用一种新型免疫原 FIP-BSA 制备了一种针对 FIP 的单克隆抗体（克隆 F-3F6），该抗体具有高亲和力和特异性，FIP-BSA 是通过将 FIP 与牛血清白蛋白（BSA）结合而简单直接合成的。在之前报道的抗体中，F-3F6 对 FIP 的作用更具特异性。FIP 的代谢物氟虫腈脱亚硫酰基、氟虫腈硫醚和氟虫腈砜的交叉反应性较低，而其他农药则无法识别。为了实现对氟虫腈的高频和现场测量，研究人员集成了蒸发波荧光技术、功能化纤维生物探针和荧光标记的 F-3F6 抗体，建立了蒸发波荧光生物传感器。FIP 的检测限为 0.032 μg/L。对真实奶样和水样的检测结果表明，所有变异系数均小于 10%，回收率在 90% 至 120% 之间。功能化纤维生物探针的高重复使用性和稳定性使其能够准确、经济、高频、方便地定量检测 FIP。这种高特异性和高可靠性的蒸发波荧光生物传感器将非常适合于对食品中仅有的 FIP 进行灵敏和高频率的现场分析。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Chemosensors Chemistry-Analytical Chemistry

CiteScore

5.00

自引率

9.50%

发文量

450

审稿时长

11 weeks

期刊介绍： Chemosensors (ISSN 2227-9040; CODEN: CHEMO9) is an international, scientific, open access journal on the science and technology of chemical sensors published quarterly online by MDPI.