Genomic Medicine, Biomarkers, and Health Sciences最新文献_第4页

Microfluidic chip with microweir structure for continuous sample separating and collecting applications 微孔结构微流控芯片用于连续样品分离和采集

Genomic Medicine, Biomarkers, and Health Sciences Pub Date : 2012-03-01 DOI: 10.1016/j.gmbhs.2012.04.013

Suz-Kai Hsiung , Ho-Cheng Lee , Pei-Seng Cheng , Yi-Fang Chen , Ping-Yu Huang , Che-Hsin Lin

{"title":"Microfluidic chip with microweir structure for continuous sample separating and collecting applications","authors":"Suz-Kai Hsiung , Ho-Cheng Lee , Pei-Seng Cheng , Yi-Fang Chen , Ping-Yu Huang , Che-Hsin Lin","doi":"10.1016/j.gmbhs.2012.04.013","DOIUrl":"10.1016/j.gmbhs.2012.04.013","url":null,"abstract":"<div><p>We aim to establish a microfluidic chip device capable for continuous sample separation by integrating a simple and easy-fabrication microweir structure for crossflow filtration in the present study. The proposed microchip device is composed of two major components, including flow channels and microweir structure. Using the injection of the mixed samples with different sizes, the samples can be transported through the flow channel and then be separated by the microweir structure. The microweir structure with a different height can be generated by utilizing a standard lithography and overetching process, so that the gap can be used to be a selective tool to separate the smaller sample. In this study, a 10-μm gap was generated by established a microweir structure with 20 μm in height with a 20-minute etching process. Optimal sample separation efficiency in 82% can be obtained of the sample concentration in 10<sup>3</sup> μL<sup>−1</sup> by utilizing the proposed design of the microweir structure to separate two groups of beads in different diameters. In conclusion, the proposed chip device can be regarded as an effective tool for clinical application.</p></div>","PeriodicalId":100577,"journal":{"name":"Genomic Medicine, Biomarkers, and Health Sciences","volume":"4 1","pages":"Pages 70-75"},"PeriodicalIF":0.0,"publicationDate":"2012-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.gmbhs.2012.04.013","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74048282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Microarray analysis and establishment of drug screening platform using 5-fluorouracil resistance HCT116 colon cancer cells 5-氟尿嘧啶耐药HCT116结肠癌细胞的芯片分析及药物筛选平台的建立

Genomic Medicine, Biomarkers, and Health Sciences Pub Date : 2012-03-01 DOI: 10.1016/j.gmbhs.2012.04.001

Ailun Heather Tseng , Feng-Hsiang Chung , Hoong-Chien Lee , Li-Ching Wu , Chang-Han Chen , Li-Jen Su

{"title":"Microarray analysis and establishment of drug screening platform using 5-fluorouracil resistance HCT116 colon cancer cells","authors":"Ailun Heather Tseng , Feng-Hsiang Chung , Hoong-Chien Lee , Li-Ching Wu , Chang-Han Chen , Li-Jen Su","doi":"10.1016/j.gmbhs.2012.04.001","DOIUrl":"10.1016/j.gmbhs.2012.04.001","url":null,"abstract":"<div><p>A systemic approach was used to identify the possible mechanisms underlying the development of 5-fluorouracil (5FU)-induced resistance on HCT116 colon cancer cells. From microarray analysis, HCT116 high-dose 5FU-resistant subclones showed differential gene expression compared to HCT116-sensitive clones. According to gene ontology, and Kyoto Encyclopedia of Genes and Genomes pathways, the up-regulated genes were related to cell death and lupus erythematosus, respectively. On the other hand, the down-regulated genes were related to cell division or DNA replication. Connectivity map (cMAP) analysis revealed that the molecular drugs, such as antiasthmatic or antiallergy agents that have negative correlations with cMAP score, may have beneficial effect for the resistant subclones. Our findings suggested that the feasibility of cMAP combining microarray gene expression profile may help identify a potential drug that possibly will reverse the effect of 5FU-induced resistance.</p></div>","PeriodicalId":100577,"journal":{"name":"Genomic Medicine, Biomarkers, and Health Sciences","volume":"4 1","pages":"Pages 21-27"},"PeriodicalIF":0.0,"publicationDate":"2012-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.gmbhs.2012.04.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87269568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Analysis of hydroxyethyl valine in hospital sterilization workers 医院消毒人员羟乙基缬氨酸含量分析

Genomic Medicine, Biomarkers, and Health Sciences Pub Date : 2012-03-01 DOI: 10.1016/j.gmbhs.2012.04.003

Kai-Hsiang Huang , Su-Yin Chiang , Chia-Fang Wu , Lan-Tyi Duann , Tsung-Jen Cheng , Kuen-Yuh Wu

引用次数: 0

Genomic Medicine, Biomarkers, and Health Sciences Pub Date : 2012-03-01 DOI: 10.1016/j.gmbhs.2012.04.009

Guan-Cheng Huang , Hsiao-Sheng Liu

引用次数: 0

DNA-dependent protein kinase regulated glioblastoma survival in doxorubicin-induced cytotoxicity dna依赖性蛋白激酶在阿霉素诱导的细胞毒性中调节胶质母细胞瘤的存活

Genomic Medicine, Biomarkers, and Health Sciences Pub Date : 2012-03-01 DOI: 10.1016/j.gmbhs.2012.04.007

Li-Hsun Lin , Hong-Lin Chan , Hsiu-Chuan Chou

{"title":"DNA-dependent protein kinase regulated glioblastoma survival in doxorubicin-induced cytotoxicity","authors":"Li-Hsun Lin , Hong-Lin Chan , Hsiu-Chuan Chou","doi":"10.1016/j.gmbhs.2012.04.007","DOIUrl":"10.1016/j.gmbhs.2012.04.007","url":null,"abstract":"<div><p>Generally, chemotherapy is effective when the cancer cell is dividing; most drugs trigger cancer cells to undergo apoptosis by attacking the cell’s DNA. In the process of cancer cell apoptosis, cancer cells become more resistant to chemotherapy treatments over time. Since DNA-dependent protein kinase (DNA-PK) plays an important role in DNA repairing, it is interesting to investigate the relationship between this particular enzyme and the development of multidrug resistance. In this study, we chose the commonly used chemotherapy drug doxorubicin to treat glioblastoma cells (M059k and M059j), and performed 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenykterazolium bromide (MTT) assay and immunofluorescence staining to assess the presence of DNA-PK. The result of MTT assay showed that the concentration of an inhibitor/drug required to reduce the cell viability by half of M059j is 1.75μm while that of M059k is 0.71μm after doxorubicin treatment. Comparing the staining result of M059j and M059k, DNA-PK was more detectable in M059k than in M059j. It suggested that further experiments need to be performed to identify and characterize the proteins that are important for signal transduction pathways that actually link DNA-PK with doxorubicin-induced cytotoxicity as well as those that are drug resistant.</p></div>","PeriodicalId":100577,"journal":{"name":"Genomic Medicine, Biomarkers, and Health Sciences","volume":"4 1","pages":"Pages 54-56"},"PeriodicalIF":0.0,"publicationDate":"2012-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.gmbhs.2012.04.007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88922970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Internal transcribed spacer sequence analysis of Angelica from different habitats 不同产地当归内部转录间隔序列分析

Genomic Medicine, Biomarkers, and Health Sciences Pub Date : 2012-03-01 DOI: 10.1016/j.gmbhs.2012.04.017

Hsiao-Chun Hao , Jia-Yuan Chang , Fu-Yen Chung

{"title":"Internal transcribed spacer sequence analysis of Angelica from different habitats","authors":"Hsiao-Chun Hao , Jia-Yuan Chang , Fu-Yen Chung","doi":"10.1016/j.gmbhs.2012.04.017","DOIUrl":"10.1016/j.gmbhs.2012.04.017","url":null,"abstract":"<div><p>The internal transcribed spacer (ITS), located between the 18S and 26S nuclear ribosomal DNA (rDNA) sequences, has a high degree of variation. Analysis of ITS sequences is commonly used to identify the authenticity of Chinese herbal medicines. The aim of this study is to analyze ITS sequences of <em>Angelica</em> from different habitats to find out whether there are differences in sequence. <em>Angelicas</em> from three habitats were used in this study, including Taiwan, Sichuan Province (China), and Gansu Province (China). DNA was extracted from Angelicas, and ITS sequences were analyzed using polymerase chain reaction (PCR) and direct sequencing. The results showed that the similarity of ITS-1 and ITS-2 rDNA sequences in <em>Angelicas</em> produced in Gansu and Sichuan Provinces is up to 100%, and that produced in Taiwan and Sichuan Province is 88% and 87%, respectively. Therefore, we could use PCR and ITS sequencing analysis to identify whether these <em>Angelicas</em> were the same strain, in order to determine their worthiness and authenticity in terms of traditional Chinese medicine.</p></div>","PeriodicalId":100577,"journal":{"name":"Genomic Medicine, Biomarkers, and Health Sciences","volume":"4 1","pages":"Pages 43-44"},"PeriodicalIF":0.0,"publicationDate":"2012-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.gmbhs.2012.04.017","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74235178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Hepatoprotective activity of Livshis, a polyherbal formulation in CCl4-induced hepatotoxic male Wistar rats: A toxicity screening approach 复方利夫氏对ccl4诱导的雄性Wistar大鼠肝毒性的保护作用:毒性筛选方法

Genomic Medicine, Biomarkers, and Health Sciences Pub Date : 2011-09-01 DOI: 10.1016/j.gmbhs.2012.03.001

Tushar Kanti Bera , Kausik Chatterjee , Debasis De , Kazi Monjur Ali , Kishalay Jana , Soumyajit Maiti , Debidas Ghosh

{"title":"Hepatoprotective activity of Livshis, a polyherbal formulation in CCl4-induced hepatotoxic male Wistar rats: A toxicity screening approach","authors":"Tushar Kanti Bera , Kausik Chatterjee , Debasis De , Kazi Monjur Ali , Kishalay Jana , Soumyajit Maiti , Debidas Ghosh","doi":"10.1016/j.gmbhs.2012.03.001","DOIUrl":"10.1016/j.gmbhs.2012.03.001","url":null,"abstract":"<div><p>This study investigated the hepatoprotective activity of the polyherbal formulation Livshis in CCl<sub>4</sub>-induced hepatotoxic male albino rats, and included an assessment of the toxicity of the formulation. Male Wistar albino rats (140 ± 10 g) were divided into five groups (<em>n</em> = 6). Liver necrosis was induced by intraperitoneal injection of CCl<sub>4</sub> (1 mL/kg body weight, 50% v/v with olive oil). Antioxidant enzyme activities, such as lipid peroxidation, and liver function test biosensors were assessed. Hematological and renotoxicity markers were evaluated to assess the general toxicity of the formulation. For acute toxicity evaluation of Livshis, the formulation was administered orally at doses ranging from 25 to 3200 mg/kg body weight. Hepatic antioxidant enzyme activities diminished significantly, and hepatic lipid peroxidation rates were elevated in CCl<sub>4</sub>-treated animals that were pretreated with distilled water (Group II). The activities of serum toxicity marker enzymes and serum liver function test biosensors increased significantly in Group II animals, whereas these biosensors were significantly protected in Livshis pretreated, CCl<sub>4</sub>-treated animals. Group V animals, treated with Livshis alone, did not exhibit any significant variation in levels of hematological and renotoxicity markers compared to controls. In an acute toxicity study, there were no toxic symptoms up to the dose level of 3200 mg/kg body weight. We conclude that Livshis is safe for long-term treatment for hepatic protection at doses of 50 mg/kg body weight.</p></div>","PeriodicalId":100577,"journal":{"name":"Genomic Medicine, Biomarkers, and Health Sciences","volume":"3 3","pages":"Pages 103-110"},"PeriodicalIF":0.0,"publicationDate":"2011-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.gmbhs.2012.03.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75017683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Birthweight of full-term twin infants in relation to sex-pair 足月双胞胎婴儿出生体重与性别对的关系

Genomic Medicine, Biomarkers, and Health Sciences Pub Date : 2011-09-01 DOI: 10.1016/j.gmbhs.2012.02.002

Alphonsus N. Onyiriuka

{"title":"Birthweight of full-term twin infants in relation to sex-pair","authors":"Alphonsus N. Onyiriuka","doi":"10.1016/j.gmbhs.2012.02.002","DOIUrl":"10.1016/j.gmbhs.2012.02.002","url":null,"abstract":"<div><p>The present study examined the effect of sex-pair on birthweight of twins. The birthweight and sex-pairs of 104 live-born full-term twin pairs were recorded and analyzed. The mean birthweight and the mean birthweight sum of a different-sex twin pair were compared with that of a same-sex twin pair. The relative incidence of low birthweight (LBW) and birthweight discordance in the different-sex twin pair and same-sex twin pair were examined. Comparing the mean birthweight of the different-sex twin pair with that of same-sex twin pair, it was 2503 ± 428 g [95% confidence interval (CI) = 2369–2637] versus 2398 ± 442 g (95% CI = 2291–2505), <em>p</em> > 0.05. The overall mean birthweight sum of the different-sex twin pair was 3889 ± 492 g (95% CI = 3679–4099) while, for the same-sex twin pair, it was 3665 ± 512 g (CI = 3493–3837), <em>p</em> > 0.05. The mean birthweight sum for the male-male twin pair was 3903 ± 536 g (95% CI = 3648–4158), while for the female-female twin pair it was 3426 ± 560 g (95% CI = 3160–3192); <em>p</em> < 0.05. The mean birthweight of female infants in the male-female twin pair was 2081 ± 492 g (95% CI = 1927–2235) compared with 1795 ± 502 g (95% CI = 1635–1955) for female infants in the female-female twin pair; <em>p</em> > 0.05. The figures for the male infants in the male-female pair and the male-male twin pair were 2136 ± 468 g (95% CI = 1989–2283) and 1976 ± 519 g (95% CI = 1804–2148), respectively; <em>p</em> > 0.05. Rate of delivery of LBW twin pairs in all live-born twins were: different-sex, 51.3%; male same-sex, 43.8%; and female same-sex, 75.8%. Of the 59 LBW twin pairs, 20 (33.9%) were different-sex and 39 (66.1%) were same-sex twin pairs. In conclusion, cohabitation in the uterus of twin fetuses of different sexes influenced their intrauterine growth.</p></div>","PeriodicalId":100577,"journal":{"name":"Genomic Medicine, Biomarkers, and Health Sciences","volume":"3 3","pages":"Pages 123-127"},"PeriodicalIF":0.0,"publicationDate":"2011-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.gmbhs.2012.02.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85389488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Antihyperlipedemic activity of Cynodon dactylon extract in high-cholesterol diet fed Wistar rats 高胆固醇饲料喂养Wistar大鼠对短爪蟹提取物的降血脂作用

Genomic Medicine, Biomarkers, and Health Sciences Pub Date : 2011-09-01 DOI: 10.1016/j.gmbhs.2011.11.001

S. Rashmi Kaup , Nayanatara Arunkumar , Leigelin Kavitha Bernhardt , Rakesh Gorantla Vasavi , Sandeep Sanjeev Shetty , Sheila Ramesh Pai , B. Arunkumar

{"title":"Antihyperlipedemic activity of Cynodon dactylon extract in high-cholesterol diet fed Wistar rats","authors":"S. Rashmi Kaup , Nayanatara Arunkumar , Leigelin Kavitha Bernhardt , Rakesh Gorantla Vasavi , Sandeep Sanjeev Shetty , Sheila Ramesh Pai , B. Arunkumar","doi":"10.1016/j.gmbhs.2011.11.001","DOIUrl":"10.1016/j.gmbhs.2011.11.001","url":null,"abstract":"<div><p>The aim of the present study was to investigate the potential role of an ethanolic extract of the entire plant of <em>Cynodon dactylon</em> in lowering the plasma lipid parameters in rats fed a high cholesterol diet. Wistar albino rats were randomly divided into four groups of six and for 45 days were administered either: 0.5ml water (negative controls); 30mg cholesterol (hypercholesterolemic animals); <em>C dactylon</em> extract at 400mg/kg body weight (positive control); or the same doses of both cholesterol and the extract (test animals). The effects of <em>C dactylon</em> on the lipid profile were assessed by measuring the plasma concentrations of total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-c), high-density lipoprotein cholesterol (HDL-c), and very low-density lipoprotein cholesterol (VLDL-c). Administration of cholesterol showed significant elevation (<em>p</em>  <0.05). Concurrent administration of <em>C dactylon</em> extract caused a significant decrease (<em>p</em>  <0.001). These results suggest lipid-lowering effects of <em>C dactylon</em>, which serves as a new potential natural product for preventing hyperlipidemia.</p></div>","PeriodicalId":100577,"journal":{"name":"Genomic Medicine, Biomarkers, and Health Sciences","volume":"3 3","pages":"Pages 98-102"},"PeriodicalIF":0.0,"publicationDate":"2011-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.gmbhs.2011.11.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77141387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 32

Preparation and evaluation of sucrose stabilized tetanus toxoid encapsulated into chitosan microspheres 壳聚糖微球壳聚糖稳定破伤风类毒素的制备及评价

Genomic Medicine, Biomarkers, and Health Sciences Pub Date : 2011-09-01 DOI: 10.1016/j.gmbhs.2011.10.001

Saravanakumar Arthanari , Ponnusamy Renukadevi , Kavaretti Raju Mani

{"title":"Preparation and evaluation of sucrose stabilized tetanus toxoid encapsulated into chitosan microspheres","authors":"Saravanakumar Arthanari , Ponnusamy Renukadevi , Kavaretti Raju Mani","doi":"10.1016/j.gmbhs.2011.10.001","DOIUrl":"10.1016/j.gmbhs.2011.10.001","url":null,"abstract":"<div><p>Immunization is the most cost effective weapon for disease prevention in developing countries, and advanced molecular and genetic technologies are making new types of vaccines feasible. Here, the utility of both <em>in vitro</em> and <em>in vivo</em> methods to assess the release pattern of chitosan microspheres containing tetanus toxoid (TT) vaccine were evaluated. TT was stabilized and encapsulated in chitosan (TTCH) with a water-in-oil-in-water (W/O/W) multiple emulsion method using sucrose as a protein stabilizer. The TTCH prepared was smooth and spherical in shape with a diameter of around 10μm. The <em>in vitro</em> release efficiency of TTCH was evaluated by differing stabilizer (sucrose) concentration (5%, 7%, 10% and 12%w/v) for a period of 70 days. The antigen release rates from the microspheres were determined by enzyme-linked immunosorbent assay. In these TTCH microspheres, a 10%w/v sucrose concentration gave good sustained antigen delivery for the period of 70 days. Based on the results of <em>in vitro</em> release, the <em>in vivo</em> studies were carried out using alum-adsorbed TT (from the Central Research Institute) as the standard. The antibody level was measured after 6 months, 9 months and finally, with one booster dose, after 12 months. In these <em>in vivo</em> studies, the TTCH antibody level rose up to 3.5IU/mL of guinea pig serum; this compared with 2IU/mL of guinea pig serum using the alum-adsorbed TT after 12 months with a second booster dose. The TTCH approach would be helpful to replace the existing adjuvant alum in the future.</p></div>","PeriodicalId":100577,"journal":{"name":"Genomic Medicine, Biomarkers, and Health Sciences","volume":"3 3","pages":"Pages 91-97"},"PeriodicalIF":0.0,"publicationDate":"2011-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.gmbhs.2011.10.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72936681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5