Frontiers in Laboratory Medicine最新文献_第3页

Research progress on anti-mullerian hormone clinical applications and immunoassay development 抗苗勒管激素临床应用及免疫分析研究进展

Frontiers in Laboratory Medicine Pub Date : 2018-03-01 DOI: 10.1016/j.flm.2018.02.002

Hui Sun , Huajie Mao , Jianhui Cai , Ya Zhao

引用次数: 3

The pathogenesis of autoimmune hepatitis 自身免疫性肝炎的发病机制

Frontiers in Laboratory Medicine Pub Date : 2018-03-01 DOI: 10.1016/j.flm.2018.03.002

Mingxue Wang, Hua Zhang

{"title":"The pathogenesis of autoimmune hepatitis","authors":"Mingxue Wang, Hua Zhang","doi":"10.1016/j.flm.2018.03.002","DOIUrl":"10.1016/j.flm.2018.03.002","url":null,"abstract":"<div><p>Autoimmune hepatitis (AIH) is a liver inflammatory disease via induction of debris-like necrosis in the liver portal area, which is characterized by hypergammaglobulinemia and a variety of autoantibodies. And its performance is very similar to viral hepatitis, but with absolutely different treatment. The pathogenesis of AIH is not clear, genetic susceptibility is considered to be the main factor. AIH is a cause of multiple pathogenic disease, and can induce viruses, bacteria, chemicals and drugs which are potential causes for genetic sensitive individuals, to participate in inflammatory disease processes. Due to the destruction of immune tolerance, liver-specific membrane proteins were expressed on the surface of liver cells, asialoglycoprotein receptor (ASGP-R) and microsomal cytochrome P4502D6 are considered to be more clearly present to stimulate AIH antigen. Activation of CD4+ T cells by direct exposure and release of cytokines stimulates B cells to produce antibodies that initiate immune injury responses. At the same time cytokine activation of CD8+ T cells mediates antibody-dependent cell-mediated cytotoxicity (ADCC) effect to kill hepatocytes, activating tumour necrosis factor (TNF) or Fas-mediated hepatocellular death. However, AIH patients are always proved to be lack of immunoregulatory function, which is newly considered to be the cardinal cause of AIH. Genetic susceptibility, molecular simulation theory, and immune pathogenesis are main points of this review article. Especially, in-depth statement has been made from several aspects of AIH’s immune pathogenesis such as cellular immune mechanism, humoral immunity with ADCC, immunosuppressive effects of regulatory T cells, and cytokine theory. Thus, a better understanding of the pathogenesis of AIH is of great importance.</p></div>","PeriodicalId":100555,"journal":{"name":"Frontiers in Laboratory Medicine","volume":"2 1","pages":"Pages 36-39"},"PeriodicalIF":0.0,"publicationDate":"2018-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.flm.2018.03.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87360330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Synthesis of chitosan mediated silver nanoparticles (Ag NPs) for potential antimicrobial applications 壳聚糖介导的银纳米粒子(Ag NPs)的合成及其抗菌应用前景

Frontiers in Laboratory Medicine Pub Date : 2018-03-01 DOI: 10.1016/j.flm.2018.04.002

R. Kalaivani , M. Maruthupandy , T. Muneeswaran , A. Hameedha Beevi , M. Anand , C.M. Ramakritinan , A.K. Kumaraguru

{"title":"Synthesis of chitosan mediated silver nanoparticles (Ag NPs) for potential antimicrobial applications","authors":"R. Kalaivani , M. Maruthupandy , T. Muneeswaran , A. Hameedha Beevi , M. Anand , C.M. Ramakritinan , A.K. Kumaraguru","doi":"10.1016/j.flm.2018.04.002","DOIUrl":"10.1016/j.flm.2018.04.002","url":null,"abstract":"<div><p>Synthesis of chitosan mediated silver nanoparticles (Ag NPs) and its characterization were fulfilled by UV–vis spectroscopy, fourier transform infrared spectroscopy, X-ray diffraction and transmission electron microscopy. UV–visible absorption spectrum revealed that the formation efficiency of Ag NPs was increased by the addition of chitosan. In addition, the size decrement of NPs was more remarkable at a higher chitosan concentration. XRD pattern has indicated that Ag NPs were spherical structured and crystalline in nature (JCPDS card no: 65-2871). The AFM and TEM images examined the surface morphology and the size of the synthesized NPs. DLS image confirmed the size distribution and range of the Ag NPs. The synthesized Ag NPs showed highly potent antibacterial and antifungal activities. The study suggested that crustacean waste could be a cheaper source for the production of Ag NPs which could be useful in various medical applications for biomedicine research and may create a new market for sea food with products such as chitin and chitosan.</p></div>","PeriodicalId":100555,"journal":{"name":"Frontiers in Laboratory Medicine","volume":"2 1","pages":"Pages 30-35"},"PeriodicalIF":0.0,"publicationDate":"2018-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.flm.2018.04.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90760709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 207

Is there a continual role for serum creatine kinase and myoglobin testing in the era of high sensitivity troponin assays? 在高灵敏度肌钙蛋白检测的时代，血清肌酸激酶和肌红蛋白检测是否有持续的作用?

Frontiers in Laboratory Medicine Pub Date : 2018-03-01 DOI: 10.1016/j.flm.2018.03.001

Alan H.B. Wu

引用次数: 1

Detection of ESBL genes from ciprofloxacin resistant Gram negative bacteria isolated from urinary tract infections (UTIs) 尿路感染中耐环丙沙星革兰氏阴性菌ESBL基因检测

Frontiers in Laboratory Medicine Pub Date : 2018-03-01 DOI: 10.1016/j.flm.2018.01.001

Govindan Rajivgandhi , Muthuchamy Maruthupandy , Govindan Ramachandran , Muthu Priyanga , Natesan Manoharan

{"title":"Detection of ESBL genes from ciprofloxacin resistant Gram negative bacteria isolated from urinary tract infections (UTIs)","authors":"Govindan Rajivgandhi , Muthuchamy Maruthupandy , Govindan Ramachandran , Muthu Priyanga , Natesan Manoharan","doi":"10.1016/j.flm.2018.01.001","DOIUrl":"10.1016/j.flm.2018.01.001","url":null,"abstract":"<div><p>Urinary tract infections (UTIs) are the most disregarded diseases in both developing and developed countries and accountable for one fourth of the health care related infections. Even though the women and children are the most susceptible reservoir, the infections are at a significant amount in men of all ages. In UTI, the multidrug resistant bacteria (MDRB) is an emerging burden and now represents a daily challenge for the management of antimicrobial therapy in healthcare settings. A total of 100 uropathogens of Gram negative bacteria (GNB) were used in the current study to detect the ciprofloxacin resistant effect due to extended spectrum beta lactameses production (ESBLs). The phenotypic identification of ESBLs producing uropathogens from UTIs were detected by primary ESBL identification test (PMIT) and double disc combination method (DDCM). In addition, the ESBL production was further confirmed by MIC stripe method. Further, the prevalence of TEM, SHV, OXA and CTX-M type genes of isolated GNB and ciprofloxacin resistant strains was detected by multiplex PCR method. Among the 100 uropathogens, 84% was found to produce ESBLs. Out of 84, 60 strains were identified as ciprofloxacin resistant by Hexa discs and they developed resistance against all antibiotics. The PMIT and DDCM proved the result including <em>Escherichia coli</em> (26), 21 isolates of <em>Proteusmirabilis</em>, 17 of <em>Pseudomonas aeruginosa</em>, 14 of <em>Klebsiellapneumoniae</em> and 6 of <em>Acinetobacter</em> sp. In particular, the high number of CTX-M and TEM genes were frequently detected from collected uropathogens and all the TEM, SHV, OXA, and CTX-M genes were identified from ciprofloxacin resistant strains only. Due to the increase of multiple ESBL genes in uropathogens, sustained supervision for using favorable antibiotics and the decreasing the infection is essential.</p></div>","PeriodicalId":100555,"journal":{"name":"Frontiers in Laboratory Medicine","volume":"2 1","pages":"Pages 5-13"},"PeriodicalIF":0.0,"publicationDate":"2018-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.flm.2018.01.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74922431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 47

Relationship between oxidative stress in patients with HBV-induced liver disease and HBV genotype/drug-resistant mutation HBV诱导肝病患者氧化应激与HBV基因型/耐药突变的关系

Frontiers in Laboratory Medicine Pub Date : 2018-02-01 DOI: 10.1016/J.FLM.2017.12.005

J. Feng, Yuwei Yang, Dong Wang, Jie Tang, G. Xie, Lingying Fan

引用次数: 2

Small cell carcinoma of the endometrium: A case report with circulating tumor cell clusters 子宫内膜小细胞癌伴循环肿瘤细胞团1例报告

Frontiers in Laboratory Medicine Pub Date : 2017-12-01 DOI: 10.1016/j.flm.2017.11.004

Yongpeng He , Hai Huang , Haixia Wang , Lin Yi , Yichao Chen , Haiwei Zhang , Jing Ran , Hesong Shen , Qingming Jiang , Weiqi Nian

{"title":"Small cell carcinoma of the endometrium: A case report with circulating tumor cell clusters","authors":"Yongpeng He , Hai Huang , Haixia Wang , Lin Yi , Yichao Chen , Haiwei Zhang , Jing Ran , Hesong Shen , Qingming Jiang , Weiqi Nian","doi":"10.1016/j.flm.2017.11.004","DOIUrl":"https://doi.org/10.1016/j.flm.2017.11.004","url":null,"abstract":"<div><p>Primary small cell carcinoma (SCC) of the endometrium is a rare neuroendocrine tumor with aggressive behavior and poor prognosis, and no effective therapeutic method and prognosis monitoring were established. Circulating tumor cell (CTC) clusters have been asscociated with increasing metastatic potential and poor prognosis in malignant tumor. Here we presented a case of a 65-year-old woman with primary SCC of the endometrium diagnosed as stage IIIC. The patient received hysterosalpingo-oophorectom, followed by chemotherapy and radiotherapy, but the condition of the patient grew worse, and rapidly distant metastasis occured in supraclavicular lymph nodes. When we detcted the number of CTC in the patient’s blood, CTC clusters were found. Moreover, plakoglobin exhibited positivity. Finally, the patient died four months after the surgery. It was speculated that CTC clusters could be a prognostic factor and therapeutic target of primary SCC of the endometrium.</p></div>","PeriodicalId":100555,"journal":{"name":"Frontiers in Laboratory Medicine","volume":"1 4","pages":"Pages 179-181"},"PeriodicalIF":0.0,"publicationDate":"2017-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.flm.2017.11.004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91725528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biological functions of miR-590 and its role in carcinogenesis miR-590的生物学功能及其在癌变中的作用

Frontiers in Laboratory Medicine Pub Date : 2017-12-01 DOI: 10.1016/j.flm.2017.11.002

Yang Dong, Guang-Bin Qiu

引用次数: 9

Graphene quantum dots in biomedical applications: Recent advances and future challenges 石墨烯量子点在生物医学中的应用:最新进展和未来挑战

Frontiers in Laboratory Medicine Pub Date : 2017-12-01 DOI: 10.1016/j.flm.2017.12.006

Fei Chen , Weiyin Gao , Xiaopei Qiu , Hong Zhang , Lianhua Liu , Pu Liao , Weiling Fu , Yang Luo

引用次数: 131

Opportunities and challenges for lung cancer screening in China 中国肺癌筛查的机遇与挑战

Frontiers in Laboratory Medicine Pub Date : 2017-12-01 DOI: 10.1016/J.FLM.2017.11.003

Chuanli Ren, Chongxu Han

引用次数: 0