Brain Organoid and Systems Neuroscience Journal最新文献_第3页

Microengineered organoids: reconstituting organ-level functions in vitro 微工程类器官:体外重建器官水平功能

Brain Organoid and Systems Neuroscience Journal Pub Date : 2023-04-25 DOI: 10.51335/organoid.2023.3.e5

S. Park, Harshita Sharma, W. Kim, Yonghyun Gwon, H. Kim, Y. Choung, Jangho Kim

引用次数: 0

Development of otic organoids and their current status 耳类器官的发展与现状

Brain Organoid and Systems Neuroscience Journal Pub Date : 2023-04-25 DOI: 10.51335/organoid.2023.3.e7

Hantai Kim, Young Sun Kim, Y. Kim, Jungho Ha, Siung Sung, J. Jang, S. Park, Jangho Kim, Kyungeun Kim, Y. Choung

{"title":"Development of otic organoids and their current status","authors":"Hantai Kim, Young Sun Kim, Y. Kim, Jungho Ha, Siung Sung, J. Jang, S. Park, Jangho Kim, Kyungeun Kim, Y. Choung","doi":"10.51335/organoid.2023.3.e7","DOIUrl":"https://doi.org/10.51335/organoid.2023.3.e7","url":null,"abstract":"The inner ear is responsible for both hearing and balance in the body, and since the initial development of otic (inner ear) organoids from mouse pluripotent stem cells (PSCs) in 2013, significant advances have been made in this field. Bone morphogenetic proteins, fibroblast growth factors, and Wnt agonists, which are signaling molecules in the early development of the inner ear, can induce PSCs into the otic fate. In the inner ear, hair cells and the surrounding supporting cells are essential for proper function and structure. Recent advancements in otic organoid research have enabled the generation of cells that closely resemble these key components. The developed otic organoids contain both hair cell-like cells and supporting cells, which have been confirmed to have the intrinsic function of those cell types. Otic organoids have been used for disease modeling and are expected to be more widely applied in various areas of research on the inner ear. However, the otic organoids developed to date remain immature. Although they mimic hair cells, their properties resemble vestibular (balance) hair cells more closely than cochlear (auditory) hair cells. The ultimate goal of research on the inner ear is hearing restoration and prevention; thus, it is essential to produce otic organoids that contain cochlear hair cells. In addition, the organ of Corti—a cell arrangement unique to the cochlea—has not yet been simulated. Along with a description of the current status of otic organoids, this review article will discuss future directions for otic organoids in inner ear research.","PeriodicalId":100198,"journal":{"name":"Brain Organoid and Systems Neuroscience Journal","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82060700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tumor spheroid-based and microtumor-based vascularized models for replicating the vascularized tumor microenvironment 基于肿瘤球体和微肿瘤的血管化模型用于复制血管化肿瘤微环境

Brain Organoid and Systems Neuroscience Journal Pub Date : 2023-03-25 DOI: 10.51335/organoid.2023.3.e6

Jiyoung Song, Jihoon Ko, Nakwon Choi, N. Jeon, Hongnam Kim

{"title":"Tumor spheroid-based and microtumor-based vascularized models for replicating the vascularized tumor microenvironment","authors":"Jiyoung Song, Jihoon Ko, Nakwon Choi, N. Jeon, Hongnam Kim","doi":"10.51335/organoid.2023.3.e6","DOIUrl":"https://doi.org/10.51335/organoid.2023.3.e6","url":null,"abstract":"BackgroundTumor vasculature is a crucial pathway for supplying nutrients and oxygen to tumors during their progression, as well as facilitating the delivery of anticancer drugs or immunotherapeutic agents. Microfluidic technology has emerged as a powerful tool in creating microenvironments within 3D cell cultures that more closely resemble in vivo conditions, by enabling precise control of fluid flow. As a result, microfluidic devices have made significant progress in replicating both the structural and functional characteristics of the tumor microenvironment in vitro. Methods and ResultsIn this study, we present two approaches for reconstructing the tumor vasculature using tumor spheroids or microtumors, with a particular focus on in vivo functional mimicry and experimental reproducibility. Tumor spheroid-based vascular models provide an observatory window into tumor vasculature centered on tumor spheroids, enabling quantitative measurement of the degree of abnormality of blood vessels developing around the tumor spheroid and the invasiveness of metastatic tumors. Microtumor-based vascular models, on the other hand, have the potential to enhance our comprehension of advanced and metastatic cancers at the single-cell level by elucidating the proliferative and metastatic capacities of tumor cells, as well as the vascular permeability that is contingent upon the subtypes of tumor cells. ConclusionOur platforms provide valuable insights into the development of novel in vitro models for studying the tumor microenvironment and advancing our understanding of cancer biology.","PeriodicalId":100198,"journal":{"name":"Brain Organoid and Systems Neuroscience Journal","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75892018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Small molecule-induced destabilization of β-catenin and RAS is the ideal strategies for suppressing colorectal cancer 小分子诱导β-catenin和RAS的失稳是抑制结直肠癌的理想策略

Brain Organoid and Systems Neuroscience Journal Pub Date : 2023-03-25 DOI: 10.51335/organoid.2023.3.e4

Yonghyo Kim, Myoung-Hee Kang, Geon‐Woo Kim, Yong-Hee Cho

{"title":"Small molecule-induced destabilization of β-catenin and RAS is the ideal strategies for suppressing colorectal cancer","authors":"Yonghyo Kim, Myoung-Hee Kang, Geon‐Woo Kim, Yong-Hee Cho","doi":"10.51335/organoid.2023.3.e4","DOIUrl":"https://doi.org/10.51335/organoid.2023.3.e4","url":null,"abstract":"Background: Mutations of adenomatous polyposis coli (APC) and KRAS play essential roles in the development of colorectal cancer (CRC) by forming an abnormal colon morphology. Despite intensive efforts to discover therapeutic strategies to re-transform cancer cells into normal cells, no effective approaches have been reported yet.Methods: In this study, we aimed to identify therapeutic strategies for inducing morphological changes of tumor organoids to structures similar to the normal intestine in ApcMin/+/KrasG12DLA2 mice by using KYA1797K, a dual inhibitor of the Wnt/β-catenin and RAS signaling pathways.Results: KYA1797K, previously identified as a dual inhibitor of the Wnt/β-catenin and RAS pathways, inhibited the growth of organoids derived from tumor cells of ApcMin/+/KrasG12DLA2 mice, with the transformation of benign tumor structures into normal structures, similar to bone morphogenetic protein 4 (BMP4), an intestinal differentiation signaling inducer.Conclusion: Given the anti-cancer effects of KYA1797K and its ability to induce morphological changes similar to those elicited by BMP4 treatment, the dual suppression of Wnt/β-catenin and RAS signaling is a potential therapy for treating CRC.","PeriodicalId":100198,"journal":{"name":"Brain Organoid and Systems Neuroscience Journal","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78875157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

One-step achievement of tumor spheroid-induced angiogenesis in a high-throughput microfluidic platform: one-step tumor angiogenesis platform 高通量微流控平台一步实现肿瘤球体诱导血管生成:一步肿瘤血管生成平台

Brain Organoid and Systems Neuroscience Journal Pub Date : 2023-02-25 DOI: 10.51335/organoid.2023.3.e3

Seonghyuk Park, Youngtaek Kim, Jihoon Ko, Jiyoung Song, Jeeyun Lee, Young-Kwon Hong, N. Jeon

{"title":"One-step achievement of tumor spheroid-induced angiogenesis in a high-throughput microfluidic platform: one-step tumor angiogenesis platform","authors":"Seonghyuk Park, Youngtaek Kim, Jihoon Ko, Jiyoung Song, Jeeyun Lee, Young-Kwon Hong, N. Jeon","doi":"10.51335/organoid.2023.3.e3","DOIUrl":"https://doi.org/10.51335/organoid.2023.3.e3","url":null,"abstract":"Research on the development of anti-cancer drugs has progressed, but the low reliability of animal experiments due to biological differences between animals and humans causes failures in the clinical process. To overcome this limitation, 3-dimensional (3D) in vitro models have been developed to mimic the human cellular microenvironment using polydimethylsiloxane (PDMS). However, due to the characteristics and limitations of PDMS, it has low efficiency and is not suitable to be applied in the preclinical testing of a drug. High-throughput microfluidic platforms fabricated by injection molding have been developed, but these platforms require a laborious process when handling spheroids. We recently developed an injection-molded plastic array 3D culture tissue platform that integrates the process from spheroid formation to reconstruction of an in vitro model with spheroids (All-in-One-IMPACT). In this study, we implemented a 3D tumor spheroid angiogenesis model in the developed platform. We analyzed the tendency for angiogenesis according to gel concentration and confirmed that angiogenesis occurred using cancer cell lines and patient-derived cancer cells (PDCs). We also administered an anti-cancer drug to the PDC tumor spheroid angiogenesis model to observe the drug’s effect on angiogenesis according to its concentration. We demonstrated that our platform can be used to study the tumor microenvironment (TME) and drug screening. We expect that this platform will contribute to further research on the complex mechanisms of the TME and predictive preclinical models.","PeriodicalId":100198,"journal":{"name":"Brain Organoid and Systems Neuroscience Journal","volume":"52 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79221225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A polymer-based artificial microenvironment for enhancing cell adhesion 一种增强细胞粘附的聚合物人工微环境

Brain Organoid and Systems Neuroscience Journal Pub Date : 2023-02-25 DOI: 10.51335/organoid.2023.3.e8

Suhan Lee, H. Park, Sang‐Keun Sung, Ju Kyung Lee, H. Kim

{"title":"A polymer-based artificial microenvironment for enhancing cell adhesion","authors":"Suhan Lee, H. Park, Sang‐Keun Sung, Ju Kyung Lee, H. Kim","doi":"10.51335/organoid.2023.3.e8","DOIUrl":"https://doi.org/10.51335/organoid.2023.3.e8","url":null,"abstract":"An efficient platform capable of cell adhesion needs to be developed to understand cell activities such as cell differentiation, diffusion, and migration. The basic sequence of cell adhesion involves cells communicating with their environment by generating mechanical and chemical signals. Thin polymeric films with micro- or nano-patterns are widely used to support cell growth with conformal contact at the biointerface. However, stable and biocompatible films with high reproducibility on a flexible substrate remain a challenge. As described here, we developed micro-pattern poly(tetrafluoroethyleneco-perfluoro-3,6-dioxa-4-methyl-7-octenesulfonic acid) (Nafion) films fabricated by a molding process. We present the fabrication and characterization of flexible, micro-patterned Nafion films and the evaluation of cell adhesion and alignment on these films. We found that cell adhesion and migration/direction could be modulated by controlling the surface architecture. This approach offers a new platform that constitutes a promising tool for use in flexible cell-based platforms and devices to observe cell-cell and cell-surface interactions.","PeriodicalId":100198,"journal":{"name":"Brain Organoid and Systems Neuroscience Journal","volume":"255 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79500996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineered adipose tissue platforms: recent breakthroughs and future perspectives 工程脂肪组织平台:最近的突破和未来的展望

Brain Organoid and Systems Neuroscience Journal Pub Date : 2023-01-25 DOI: 10.51335/organoid.2023.3.e1

Heejeong Yoon, Tae-Eun Park

引用次数: 0

Induced pluripotent stem cell-derived hematopoietic stem and progenitor cells: potential, challenges, and future perspectives 诱导多能干细胞衍生的造血干细胞和祖细胞:潜力、挑战和未来展望

Brain Organoid and Systems Neuroscience Journal Pub Date : 2023-01-25 DOI: 10.51335/organoid.2023.3.e2

Myoung Hee Han, Da-Hyun Kim, Kyung-Rok Yu

{"title":"Induced pluripotent stem cell-derived hematopoietic stem and progenitor cells: potential, challenges, and future perspectives","authors":"Myoung Hee Han, Da-Hyun Kim, Kyung-Rok Yu","doi":"10.51335/organoid.2023.3.e2","DOIUrl":"https://doi.org/10.51335/organoid.2023.3.e2","url":null,"abstract":"Hematopoietic stem and progenitor cells (HSPCs) are responsible for the lifetime dynamics of hematopoiesis, as they are well known for their self-renewing ability and multipotency to differentiate into all types of blood cells, including both myeloid and lymphoid lineages. However, due to their limited amount and accessibility, there is a strong need to search out alternative methods to produce HSPCs. In this review, we suggest induced pluripotent stem cells (iPSCs) as a new viable source for HSPC production because these cells have the potential to self-renew while being relatively easy to modify. Recent studies have revealed that the recapitulation of definitive hematopoiesis is the key to the successful in vitro production of HSPCs with multilineage potential. Therefore, we summarized recent progress in establishing the generation of definitive HSPCs with high maturity and functionality in vitro. Definitive HSPCs can be used in disease modeling and gene therapy for genetic blood disorders via gene modification in iPSCs, applied in cellular immunotherapy in the form of a universal chimeric antigen receptor system, and may recapitulate the intricate immune system within the iPSC-derived organoids that closely mimic the in vivo pathophysiological environment. In summary, this review provides an overview of the generation of HSPCs from iPSCs, in terms of the developmental process of hematopoiesis, in vitro attempts to produce iPSC-derived definitive HSPCs, and the following applications of these cells in numerous areas. This review sheds light on the concept of iPSC-derived definitive HSPCs, setting a milestone for artificial blood production in the near future.","PeriodicalId":100198,"journal":{"name":"Brain Organoid and Systems Neuroscience Journal","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75464493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid anticancer drug screening using patient-derived lung cancer organoids: a case report 使用患者来源的肺癌类器官进行快速抗癌药物筛选:1例报告

Brain Organoid and Systems Neuroscience Journal Pub Date : 2022-12-25 DOI: 10.51335/organoid.2022.2.e10

Hyeong Jun Cho, Jeong Uk Lim, S. Kim, Y. Hwang, Jong Y. Park, Dong Woo Lee, C. Yeo

{"title":"Rapid anticancer drug screening using patient-derived lung cancer organoids: a case report","authors":"Hyeong Jun Cho, Jeong Uk Lim, S. Kim, Y. Hwang, Jong Y. Park, Dong Woo Lee, C. Yeo","doi":"10.51335/organoid.2022.2.e10","DOIUrl":"https://doi.org/10.51335/organoid.2022.2.e10","url":null,"abstract":"A variety of anticancer drugs and targeted agents for lung cancer have been developed, but some patients do not respond to these medications as intended. Therefore, there is an urgent need to develop a tool for predicting the anticancer drug response of each patient. Patient-derived tumor organoids (PDOs) have emerged as reliable in vitro tools for developing precision medicine. Herein, we describe a case of a 50-year-old nonsmoking man who was diagnosed with non-small cell lung cancer. Initially, no clinical symptoms were found in this patient. Postoperative pathology confirmed a stage Ib tumor, and an epidermal growth factor receptor (EGFR) mutation (exon21p.L858R) was detected in the patient’s lung tumor specimen. The patient later showed intracranial relapse 17 months after complete resection. An organoid culture was established from the resected brain metastatic tissue and a drug sensitivity test showed, within 72 hours, that the organoids were resistant to gefitinib and osimertinib. Our results recapitulated the patient’s response to anticancer drugs, demonstrating the potential of PDOs for precision medicine.","PeriodicalId":100198,"journal":{"name":"Brain Organoid and Systems Neuroscience Journal","volume":"52 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91251125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gastric stem cell research and gastric organoids 胃干细胞研究和胃类器官

Brain Organoid and Systems Neuroscience Journal Pub Date : 2022-12-06 DOI: 10.51335/organoid.2022.2.e27

Haengdueng Jeong, K. Nam

{"title":"Gastric stem cell research and gastric organoids","authors":"Haengdueng Jeong, K. Nam","doi":"10.51335/organoid.2022.2.e27","DOIUrl":"https://doi.org/10.51335/organoid.2022.2.e27","url":null,"abstract":"The stomach is a complex organ lined with ordered epithelium consisting of different adult stem cell (ASC) pools. In the previous decade, research into gastric epithelial stem cells has been performed using lineage tracing methods, and several putative ASC markers in the gastric gland have been identified, although their roles in homeostasis maintenance and the origin of cancer remain to be clarified. With advances in gastric stem cell research, 3-dimensional (3D) organoid culture has been developed on the basis of in-depth insights into the control of stem cell self-renewal, proliferation, and differentiation. Since the initial report that single intestinal stem cells have the ability to generate long-lived 3D structures that exhibit budding forms and self-renewal, tissue-specific adaptations of this method have been established in various organs, such as the small intestine, colon, liver, and stomach. In the murine stomach, putative ASCs isolated from the corpus and antrum generate gastric organoids that can simulate organ-specific cells to some extent. In addition, a few trials have been conducted to generate long-lived 3D organoids using human-derived ASCs and pluripotent stem cells. We hope that this review will provide comprehensive knowledge on gastric stem cell research and gastric organoids.","PeriodicalId":100198,"journal":{"name":"Brain Organoid and Systems Neuroscience Journal","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73870305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0