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Signal sinc-interpolation: a fast computer algorithm 信号自插补:一种快速计算机算法
Bioimaging Pub Date : 1996-12-01 DOI: 10.1002/1361-6374(199612)4:4<225::AID-BIO1>3.0.CO;2-G
L. Yaroslavsky
{"title":"Signal sinc-interpolation: a fast computer algorithm","authors":"L. Yaroslavsky","doi":"10.1002/1361-6374(199612)4:4<225::AID-BIO1>3.0.CO;2-G","DOIUrl":"https://doi.org/10.1002/1361-6374(199612)4:4<225::AID-BIO1>3.0.CO;2-G","url":null,"abstract":"An efficient algorithm for discrete signal sinc-interpolation that is suitable for use in image and signal processing is described. Being mathematically equivalent to the commonly used zero padding interpolation method, the algorithm surpasses it in terms of flexibility, computational complexity and usage of computer memory.","PeriodicalId":100176,"journal":{"name":"Bioimaging","volume":"12 1","pages":"225-231"},"PeriodicalIF":0.0,"publicationDate":"1996-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79613979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 31
Systematic detection of subtle spatio–temporal patterns in time‐lapse imaging: I. Mitosis 延时成像中细微时空模式的系统检测:1 .有丝分裂
Bioimaging Pub Date : 1996-12-01 DOI: 10.1002/1361-6374(199612)4:4<232::AID-BIO2>3.0.CO;2-L
R. Valdés-Pérez
{"title":"Systematic detection of subtle spatio–temporal patterns in time‐lapse imaging: I. Mitosis","authors":"R. Valdés-Pérez","doi":"10.1002/1361-6374(199612)4:4<232::AID-BIO2>3.0.CO;2-L","DOIUrl":"https://doi.org/10.1002/1361-6374(199612)4:4<232::AID-BIO2>3.0.CO;2-L","url":null,"abstract":"A recent article on syncytial nuclear divisions in early embryos of Drosophila melanogaster presented evidence that the complex spatio–temporal data obtained from time-lapse images were patterned, contrary to previous suggestions based on preliminary observations. However, the reasoning that led to the hypothesis of patterning was informal and unsystematic. Here, we propose a systematic and computerized method for detecting subtle spatio–temporal mitotic patterns, under a general formulation of mitosis as three-dimensional asynchronous processes, of which the earlier data are a special case. The approach involves a rather elaborate application of the concept of permutation test from nonparametric statistics. Far from being limited to mitotic processes, the approach holds general promise for other classes of mass, time-lapse imaging phenomena in cell and developmental biology, such as migration and cell death.","PeriodicalId":100176,"journal":{"name":"Bioimaging","volume":"2010 1","pages":"232-242"},"PeriodicalIF":0.0,"publicationDate":"1996-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86272718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Bio-speckle flowmetry for retinal blood flow diagnostics 生物斑点血流法用于视网膜血流诊断
Bioimaging Pub Date : 1996-12-01 DOI: 10.1002/1361-6374(199612)4:4<254::AID-BIO4>3.0.CO;2-7
Y. Aizu, T. Asakura, K. Ogino, T. Sugita, Yasuyuki Suzuki, K. Masuda
{"title":"Bio-speckle flowmetry for retinal blood flow diagnostics","authors":"Y. Aizu, T. Asakura, K. Ogino, T. Sugita, Yasuyuki Suzuki, K. Masuda","doi":"10.1002/1361-6374(199612)4:4<254::AID-BIO4>3.0.CO;2-7","DOIUrl":"https://doi.org/10.1002/1361-6374(199612)4:4<254::AID-BIO4>3.0.CO;2-7","url":null,"abstract":"Bio-speckle flowmetry for measuring the retinal blood flow velocity is described. The measuring principle is briefly discussed in comparison with the laser Doppler method. The basic properties of the photon correlation measurements including reproducibility were experimentally investigated with a rotating ground glass disk and for the normal human retina. The error was estimated to be less than 20% for in vivo measurements. By using a glass capillary model, the reciprocal of correlation time was calibrated to the mean flow velocity with a consideration of the effects of the vessel diameter and the background reflectance. The blood flow volume rate in the human retina was estimated by using the calibrated velocity and the vessel diameter. The results compared well with those reported in the literature, and show the usefulness of this flowmetry for clinical diagnostic purposes.","PeriodicalId":100176,"journal":{"name":"Bioimaging","volume":"5 1","pages":"254-267"},"PeriodicalIF":0.0,"publicationDate":"1996-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89638506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 19
Laser speckle spectroscopy—a new method for using small swimming organisms as biomonitors 激光散斑光谱法——一种利用小型游泳生物作为生物监测器的新方法
Bioimaging Pub Date : 1996-12-01 DOI: 10.1002/1361-6374(199612)4:4<243::AID-BIO3>3.0.CO;2-E
J A Cole, M H Tinker
{"title":"Laser speckle spectroscopy—a new method for using small swimming organisms as biomonitors","authors":"J A Cole,&nbsp;M H Tinker","doi":"10.1002/1361-6374(199612)4:4<243::AID-BIO3>3.0.CO;2-E","DOIUrl":"https://doi.org/10.1002/1361-6374(199612)4:4<243::AID-BIO3>3.0.CO;2-E","url":null,"abstract":"<p>A novel method has been devised for the study of swimming organisms by using speckle patterns produced by their scattering of coherent laser light. The speckle patterns show fluctuations in space and time which may be correlated with the activity of the organisms. The fluctuations give an immediate indication of mobility and a more detailed analysis of the frequency spectrum of the speckle fluctuations shows characteristic resonance-like features which are specific to the organism. The speckle patterns produced by several protozoans, including <i>Paramecium bursaria, Entosiphon sulcatum</i>, and by the alga <i>Chlamydomonas reinhardii</i> and the rotifer <i>Brachionus calyciflorus</i> have been studied. Laser speckle spectroscopy (LSS) allows a rapid non-invasive monitoring of the activity of the organisms and could find application in ecotoxicity studies and environmental biomonitoring. The results presented here are the first reports of LSS and its use in this way and demonstrate its viability and potential for further development.</p>","PeriodicalId":100176,"journal":{"name":"Bioimaging","volume":"4 4","pages":"243-253"},"PeriodicalIF":0.0,"publicationDate":"1996-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/1361-6374(199612)4:4<243::AID-BIO3>3.0.CO;2-E","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72338566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Laser speckle spectroscopy—a new method for using small swimming organisms as biomonitors 激光散斑光谱——一种利用小型游动生物作为生物监测仪的新方法
Bioimaging Pub Date : 1996-12-01 DOI: 10.1002/1361-6374(199612)4:4<243::AID-BIO3>3.0.CO;2-E
J. Cole, M. Tinker
{"title":"Laser speckle spectroscopy—a new method for using small swimming organisms as biomonitors","authors":"J. Cole, M. Tinker","doi":"10.1002/1361-6374(199612)4:4<243::AID-BIO3>3.0.CO;2-E","DOIUrl":"https://doi.org/10.1002/1361-6374(199612)4:4<243::AID-BIO3>3.0.CO;2-E","url":null,"abstract":"A novel method has been devised for the study of swimming organisms by using speckle patterns produced by their scattering of coherent laser light. The speckle patterns show fluctuations in space and time which may be correlated with the activity of the organisms. The fluctuations give an immediate indication of mobility and a more detailed analysis of the frequency spectrum of the speckle fluctuations shows characteristic resonance-like features which are specific to the organism. The speckle patterns produced by several protozoans, including Paramecium bursaria, Entosiphon sulcatum, and by the alga Chlamydomonas reinhardii and the rotifer Brachionus calyciflorus have been studied. Laser speckle spectroscopy (LSS) allows a rapid non-invasive monitoring of the activity of the organisms and could find application in ecotoxicity studies and environmental biomonitoring. The results presented here are the first reports of LSS and its use in this way and demonstrate its viability and potential for further development.","PeriodicalId":100176,"journal":{"name":"Bioimaging","volume":"25 1","pages":"243-253"},"PeriodicalIF":0.0,"publicationDate":"1996-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77847281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Systematic detection of subtle spatio–temporal patterns in time-lapse imaging: I. Mitosis 延时成像中细微时空模式的系统检测：I.有丝分裂
Bioimaging Pub Date : 1996-12-01 DOI: 10.1002/1361-6374(199612)4:4<232::AID-BIO2>3.0.CO;2-L
Raúl E Valdés-Pérez
{"title":"Systematic detection of subtle spatio–temporal patterns in time-lapse imaging: I. Mitosis","authors":"Raúl E Valdés-Pérez","doi":"10.1002/1361-6374(199612)4:4<232::AID-BIO2>3.0.CO;2-L","DOIUrl":"https://doi.org/10.1002/1361-6374(199612)4:4<232::AID-BIO2>3.0.CO;2-L","url":null,"abstract":"<p>A recent article on syncytial nuclear divisions in early embryos of <i>Drosophila melanogaster</i> presented evidence that the complex spatio–temporal data obtained from time-lapse images were <i>patterned</i>, contrary to previous suggestions based on preliminary observations. However, the reasoning that led to the hypothesis of patterning was informal and unsystematic. Here, we propose a systematic and computerized method for detecting subtle spatio–temporal mitotic patterns, under a general formulation of mitosis as three-dimensional asynchronous processes, of which the earlier data are a special case. The approach involves a rather elaborate application of the concept of permutation test from nonparametric statistics. Far from being limited to mitotic processes, the approach holds general promise for other classes of mass, time-lapse imaging phenomena in cell and developmental biology, such as migration and cell death.</p>","PeriodicalId":100176,"journal":{"name":"Bioimaging","volume":"4 4","pages":"232-242"},"PeriodicalIF":0.0,"publicationDate":"1996-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/1361-6374(199612)4:4<232::AID-BIO2>3.0.CO;2-L","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72338565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Signal sinc-interpolation: A fast computer algorithm 信号sinc插值：一种快速的计算机算法
Bioimaging Pub Date : 1996-12-01 DOI: 10.1002/1361-6374(199612)4:4<225::AID-BIO1>3.0.CO;2-G
L P Yaroslavsky
{"title":"Signal sinc-interpolation: A fast computer algorithm","authors":"L P Yaroslavsky","doi":"10.1002/1361-6374(199612)4:4<225::AID-BIO1>3.0.CO;2-G","DOIUrl":"https://doi.org/10.1002/1361-6374(199612)4:4<225::AID-BIO1>3.0.CO;2-G","url":null,"abstract":"<p>An efficient algorithm for discrete signal sinc-interpolation that is suitable for use in image and signal processing is described. Being mathematically equivalent to the commonly used zero padding interpolation method, the algorithm surpasses it in terms of flexibility, computational complexity and usage of computer memory.</p>","PeriodicalId":100176,"journal":{"name":"Bioimaging","volume":"4 4","pages":"225-231"},"PeriodicalIF":0.0,"publicationDate":"1996-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/1361-6374(199612)4:4<225::AID-BIO1>3.0.CO;2-G","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72338568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 31
Avalanche photodiode detection with object scanning and image restoration provides 2–4 fold resolution increase in two‐photon fluorescence microscopy 雪崩光电二极管检测与对象扫描和图像恢复提供2-4倍的分辨率增加在双光子荧光显微镜
Bioimaging Pub Date : 1996-09-01 DOI: 10.1002/1361-6374(199609)4:3<187::AID-BIO9>3.0.CO;2-3
H. Kano, H. M. Voort, M. Schrader, Geert M. P. van Kempen, S. Hell
{"title":"Avalanche photodiode detection with object scanning and image restoration provides 2–4 fold resolution increase in two‐photon fluorescence microscopy","authors":"H. Kano, H. M. Voort, M. Schrader, Geert M. P. van Kempen, S. Hell","doi":"10.1002/1361-6374(199609)4:3<187::AID-BIO9>3.0.CO;2-3","DOIUrl":"https://doi.org/10.1002/1361-6374(199609)4:3<187::AID-BIO9>3.0.CO;2-3","url":null,"abstract":"High-quantum-efficiency photodetection, millisecond pixel dwell time stage scanning and image restoration by maximum-likelihood estimation are synergetically combined and shown to improve the resolution of two-photon excitation microscopy 2–4 fold in all directions. Measurements of the two-photon excitation point-spread function (PSF) of a 1.4 aperture oil immersion lens are carried out by imaging fluorescence beads with a diameter of one seventh of the excitation wavelength (830 nm) and subsequent deconvolution with the bead object function. The proposed method of resolution increase is applied to beads as well as to rhodamine labelled actin fibres in mouse fibroblast cells. As the resolution improvement is not based on the non-linear effect of two-photon excitation, the results imply a comparable resolution increase in single-photon excitation confocal microscopy. In the fibroblasts, we established a three-fold improvement in axial resolution, namely from 840 nm before, to 280 nm after restoration (full-width at half-maximum). Actin fibres with axial distances of 850 nm, otherwise difficult to discern, are fully separated. In the lateral direction, images of fluorescence beads of about 110 nm diameter are restored to the real dimensions of the beads with an accuracy of better than one pixel (41 nm).","PeriodicalId":100176,"journal":{"name":"Bioimaging","volume":"42 1","pages":"187-197"},"PeriodicalIF":0.0,"publicationDate":"1996-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81519678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 35
Effect of the detector size and the fluorescence wavelength on the resolution of three‐ and two‐photon confocal microscopy 探测器尺寸和荧光波长对三光子和二光子共聚焦显微镜分辨率的影响
Bioimaging Pub Date : 1996-09-01 DOI: 10.1002/1361-6374(199609)4:3<129::AID-BIO3>3.0.CO;2-L
M. Gu, X. Gan
{"title":"Effect of the detector size and the fluorescence wavelength on the resolution of three‐ and two‐photon confocal microscopy","authors":"M. Gu, X. Gan","doi":"10.1002/1361-6374(199609)4:3<129::AID-BIO3>3.0.CO;2-L","DOIUrl":"https://doi.org/10.1002/1361-6374(199609)4:3<129::AID-BIO3>3.0.CO;2-L","url":null,"abstract":"","PeriodicalId":100176,"journal":{"name":"Bioimaging","volume":"57 1","pages":"129-137"},"PeriodicalIF":0.0,"publicationDate":"1996-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80431736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 13
Two‐photon laser scanning fluorescence microscopy‐from a fluorophore and specimen perspective 双光子激光扫描荧光显微镜-从荧光团和标本的角度
Bioimaging Pub Date : 1996-09-01 DOI: 10.1002/1361-6374(199609)4:3<168::AID-BIO7>3.0.CO;2-8
J. Bhawalkar, A. Shih, Sinno Jialin Pan, W. Liou, J. Swiatkiewicz, B. Reinhardt, P. N. Prasad, P. Cheng
{"title":"Two‐photon laser scanning fluorescence microscopy‐from a fluorophore and specimen perspective","authors":"J. Bhawalkar, A. Shih, Sinno Jialin Pan, W. Liou, J. Swiatkiewicz, B. Reinhardt, P. N. Prasad, P. Cheng","doi":"10.1002/1361-6374(199609)4:3<168::AID-BIO7>3.0.CO;2-8","DOIUrl":"https://doi.org/10.1002/1361-6374(199609)4:3<168::AID-BIO7>3.0.CO;2-8","url":null,"abstract":"","PeriodicalId":100176,"journal":{"name":"Bioimaging","volume":"48 1","pages":"168-178"},"PeriodicalIF":0.0,"publicationDate":"1996-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82236595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 24
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