Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis最新文献

Differential stimulation by polyamines of phage RNA-directed synthesis of proteins 多胺对噬菌体rna定向合成蛋白的差异刺激

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis Pub Date : 1981-12-28 DOI: 10.1016/0005-2787(81)90078-2

Yasuhiro Watanabe, Kazuei Igarashi, Seiyu Hirose

引用次数: 34

Novel DNA sequence organization in rice genome 水稻基因组中新的DNA序列组织

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis Pub Date : 1981-12-28 DOI: 10.1016/0005-2787(81)90080-0

Vidya S. Gupta, S.R. Gadre, P.K. Ranjekar

引用次数: 18

S1 nuclease does not cleave DNA at single-base mis-matches S1核酸酶不切割单碱基不匹配的DNA

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis Pub Date : 1981-12-28 DOI: 10.1016/0005-2787(81)90094-0

John R. Silber, Lawrence A. Loeb

{"title":"S1 nuclease does not cleave DNA at single-base mis-matches","authors":"John R. Silber, Lawrence A. Loeb","doi":"10.1016/0005-2787(81)90094-0","DOIUrl":"10.1016/0005-2787(81)90094-0","url":null,"abstract":"<div>Three assays have been designed to detect the cleavage of duplex ΦX174 DNA at single-base mis-matches. Studies with S1 nuclease failed to detect cleavage at mis-matches. S1 nuclease digestion at 37 and 55°C failed to produce a preferential degradation of a multiply mis-matched heteroduplex when compared to a mis-match-free homoduplex as analyzed by sedimentation on sucrose gradients. Other heteroduplex templates were not cleaved by S1 nuclease at a defined single-base mis-match when assayed by gel electrophoresis or by marker rescue. In all cases, the amount of S1 nuclease employed was at least 10-times more than that required to render a single-stranded ΦX174 DNA molecule completely acid soluble. The rate of hydrolysis of single-base mis-matches by S1 nuclease was estimated to be less than 0.016% of the rate at a base in single-strand ΦX174 DNA. In no instance did we detect activity by S1 nuclease directed at mis-matched sites in our template molecules. Similarly, the single-strand specific endonuclease from Neurospora crassa does not cleave heteroduplex templates at a defined single-base mismatch when assayed by marker rescue.</div>","PeriodicalId":100164,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis","volume":"656 2","pages":"Pages 256-264"},"PeriodicalIF":0.0,"publicationDate":"1981-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0005-2787(81)90094-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17336937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 35

Purification and properties of fMet-tRNAf deacylase from Escherichia coli 大肠杆菌中fMet-tRNAf脱羧酶的纯化及性能研究

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis Pub Date : 1981-12-28 DOI: 10.1016/0005-2787(81)90092-7

Kazuei Igarashi, Mika Matsunaka, Keiko Kashiwagi, Kazuhiro Mitsui, Seiyu Hirose

{"title":"Purification and properties of fMet-tRNAf deacylase from Escherichia coli","authors":"Kazuei Igarashi, Mika Matsunaka, Keiko Kashiwagi, Kazuhiro Mitsui, Seiyu Hirose","doi":"10.1016/0005-2787(81)90092-7","DOIUrl":"10.1016/0005-2787(81)90092-7","url":null,"abstract":"<div>A formylmethionyl-tRNAf deacylase has been purified about 330-fold from a crude initiation factor preparation (1 M NH4Cl ribosomal wash) from Escherichia coli Q13. The enzyme was nearly homogeneous and had an apparent molecular weight of 24 000. Rat liver methionyl-tRNAf and E. coli methionyl-tRNAm were not hydrolyzed significantly by the enzyme under standard conditions. Qβ RNA- and AUG(A)n-directed polypeptide synthesis was inhibited by the enzyme. The inhibition was at the level of initiation of polypeptide synthesis. The enzymatic activity was inhibited by various factors necessary for polypeptide synthesis. The activity was inhibited more by NH4Cl and spermidine than by Mg2+, GTP and ATP. The complex of formylmethionyl-tRNAf, initiation factor 2 and GTP was resistant to enzymatic hydrolysis, and the resistance was enhanced by the addition of AUG and ribosomes to the above reaction mixture.</div>","PeriodicalId":100164,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis","volume":"656 2","pages":"Pages 240-245"},"PeriodicalIF":0.0,"publicationDate":"1981-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0005-2787(81)90092-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18079904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Characterization of five members of the actin gene family in the sea urchin 海胆肌动蛋白基因家族的五个成员的特征

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis Pub Date : 1981-12-28 DOI: 10.1016/0005-2787(81)90087-3

Paul A Overbeek , Glenn T Merlino , N Kent Peters, Vivian H Cohn, Gordon P Moore, Lewis J Kleinsmith

{"title":"Characterization of five members of the actin gene family in the sea urchin","authors":"Paul A Overbeek , Glenn T Merlino , N Kent Peters, Vivian H Cohn, Gordon P Moore, Lewis J Kleinsmith","doi":"10.1016/0005-2787(81)90087-3","DOIUrl":"10.1016/0005-2787(81)90087-3","url":null,"abstract":"<div>Hybridization of an actin cDNA clone (pSA38) to restriction enzyme digests of Strongylocentrotus purpuratus DNA indicates that the sea urchin genome contains at least five different actin genes. A sea urchin genomic clone library was screened for recombinants which hydridize to pSA38 and four genomic clones were isolated. Restriction maps were generated which indicate that three of these recombinants contain different actin genes, and that the fourth may be an allele to one of these. The restriction maps suggest that one clone contains two linked actin genes. This fact, which was confirmed by heteroduplex analysis, indicates that the actin gene family may be clustered. The linked genes are oriented in the same direction and spaced about 8.0 kilobases apart. In heteroduplexes between genomic clones two intervening sequences were seen. Significant homology is confined to the actin coding region and does not include any flanking sequence. Southern blot analysis reveals that repetitive DNA sequences are found in the region of the actin genes.</div>","PeriodicalId":100164,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis","volume":"656 2","pages":"Pages 195-205"},"PeriodicalIF":0.0,"publicationDate":"1981-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0005-2787(81)90087-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17237671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 34

Influence of inorganic cations and histone proteins on the terbium(III)-nucleic acid interaction 无机阳离子和组蛋白对铽-核酸相互作用的影响

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis Pub Date : 1981-12-28 DOI: 10.1016/0005-2787(81)90083-6

David S. Gross , Scott W. Rice , Henry Simpkins

{"title":"Influence of inorganic cations and histone proteins on the terbium(III)-nucleic acid interaction","authors":"David S. Gross , Scott W. Rice , Henry Simpkins","doi":"10.1016/0005-2787(81)90083-6","DOIUrl":"10.1016/0005-2787(81)90083-6","url":null,"abstract":"<div>We have studied the interaction of the fluorescent lanthanide, terbium(III) (Tb3+), with polynucleotides and linear and superhelical DNA, through employment of mono- and multivalent cations as competitive inhibitors. Increasingly effective competitive inhibition of the Tb3+-nucleic acid interaction was achieved, for the most part, in the cation order <math><mtext>monovalent < divalent < tetravalent</mtext></math>. The divalent cation Cu2+ proved to be an exception to this trend, and was the strongest competitive inhibitor of all cations tested, exhibiting an affinity for Tb3+ binding sites over twice that of Tb3+ itself. Unexpectedly, a narrow range of low sodium ion concentration (8–20 mM) was found to be effective in inducing localized unwinding or unstacking of linear and supercoiled DNA double helices, a phenomenon detectable through the use of both Tb3+ fluorescence enhancement and ultraviolet spectroscopy. Within a similar range of low sodium ion concentration, moreover, histone H1 was substantially more effective in displacing terbium ion from DNA than either histones H2B or H4, but at higher ionic strength, this difference was absent. These results further confirm the sensitivity and specificity of Tb3+ as a conformational probe of nucleic acids.</div>","PeriodicalId":100164,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis","volume":"656 2","pages":"Pages 167-176"},"PeriodicalIF":0.0,"publicationDate":"1981-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0005-2787(81)90083-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18330551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Ni2+, a new inhibitor of mitochondrial calcium transport 一种新的线粒体钙转运抑制剂Ni2+

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis Pub Date : 1981-12-28 DOI: 10.1016/0005-2787(81)90084-8

Erzsebet Lighti , Judit Bodnar , Eva Karoly , Erno Lindner

{"title":"Ni2+, a new inhibitor of mitochondrial calcium transport","authors":"Erzsebet Lighti , Judit Bodnar , Eva Karoly , Erno Lindner","doi":"10.1016/0005-2787(81)90084-8","DOIUrl":"10.1016/0005-2787(81)90084-8","url":null,"abstract":"<div>1. The effect of Ni2+ on respiration, volume changes and Ca2+ movements was investigated in rat liver mitochondria. 2. Ni2+ inhibited Ca2+ uptake into respiring mitochondria, Ca2+-stimulated respiration and swelling in Ca2+ salts, whereas it did not inhibit either state 4 and NDP-stimulated respiration, or swelling in K+ salt in the presence of valinomycin. 3. The inhibitory concentration of Ni2+ depended strongly on the applied Ca2+ concentration. As revealed by direct methods, 50% inhibition of Ca2+ influx was achieved by approx. 2-fold excess of Ni2+. 4. If added to Ca2+-loaded mitochondria, Ni2+ gave rise to slow Ca2+ release and inhibited uncoupler-induced efflux slightly. 5. It is concluded that Ni2+ is a potent inhibitor of mitochondrial Ca2+ transport. Ca2+ influx is far more sensitive to inhibition than Ca2+ efflux.</div>","PeriodicalId":100164,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis","volume":"656 2","pages":"Pages 177-182"},"PeriodicalIF":0.0,"publicationDate":"1981-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0005-2787(81)90084-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18330552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

Purification and properties of a neutral endodeoxyribonuclease from guinea pig epidermis 豚鼠表皮中性内脱氧核糖核酸酶的纯化及性质研究

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis Pub Date : 1981-12-28 DOI: 10.1016/0005-2787(81)90085-X

Motoaki Anai , Mieko Sasaki , Atsuko Muta , Teruo Miyagawa

{"title":"Purification and properties of a neutral endodeoxyribonuclease from guinea pig epidermis","authors":"Motoaki Anai , Mieko Sasaki , Atsuko Muta , Teruo Miyagawa","doi":"10.1016/0005-2787(81)90085-X","DOIUrl":"10.1016/0005-2787(81)90085-X","url":null,"abstract":"<div>Guinea pig epidermal DNAase I was purified from an epidermal extract by a procedure including DEAE-cellulose chromatography, Sephadex G-100 gel filtration and Con A-Sepharose affinity chromatography. The purified enzyme contained no detectable activities of acid DNAase, alkaline RNAase, phosphodiesterase or acid or alkaline phosphatase, but was contaminated with acid RNAase activity. The molecular weight of the enzyme was estimated to be 33 000 by sucrose density gradient centrifugation and Sephadex G-100 gel filtration. Its isoelectric point is <math><mtext>5.2 ± 0.1</mtext></math>. The enzyme requires divalent cations and exhibits two pH optima that are dependent on divalent cations: in the presence of Mn2+, the optimum pH is about 7.5 in 50 mM Tris-HCI buffer and in the presence of Mn2+, the pH optimum is 6.4 in 50 mM cacodylate-HCI buffer. The enzyme hydrolyzes native DNA about 6-times faster than denatured DNA, producing 5′-phosphoryl and 3′-hydroxyl terminated oligonucleotides with an average chain length of about eight nucleotides, and converts double-stranded and circular DNA to relaxed and linear forms. The enzyme is inhibited by G-actin and antiserum against bovine pancreatic DNAase A. Thus this enzyme is classified as DNAase I.</div>","PeriodicalId":100164,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis","volume":"656 2","pages":"Pages 183-188"},"PeriodicalIF":0.0,"publicationDate":"1981-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0005-2787(81)90085-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17336935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

In vitro conversion of S13 viral DNA in phage particles to the double-stranded DNA 噬菌体颗粒中S13病毒DNA向双链DNA的体外转化

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis Pub Date : 1981-12-28 DOI: 10.1016/0005-2787(81)90086-1

Kounosuke Watabe, Mamoru Kubota, Junji Morita, Tohru Komano

引用次数: 1

Poly(5-methoxycytidylic acid) 保利(5-methoxycytidylic酸)

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis Pub Date : 1981-12-28 DOI: 10.1016/0005-2787(81)90077-0

Cho Fat Hui, David W. Hutchinson

引用次数: 1