噬菌体颗粒中S13病毒DNA向双链DNA的体外转化

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis Pub Date : 1981-12-28 DOI:10.1016/0005-2787(81)90086-1

Kounosuke Watabe, Mamoru Kubota, Junji Morita, Tohru Komano

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引用次数: 1

摘要

用溶菌酶和非离子清洁剂brij58裂解大肠杆菌H560 (S13S, polA, endA)细胞提取液，观察了S13完整噬菌体颗粒中单链DNA向双链复制形式DNA的转化。该DNA产物与一种快速沉积组分相关，经沉积分析鉴定为RFII-DNA带间隙。n -乙基马来酰亚胺对转化有抑制作用，利福平、烟酰胺单核苷酸和多粘菌素b对转化无抑制作用。dna基因产物参与了复制系统。从抗s13大肠杆菌菌株K12W6中提取的类似提取物也催化了该合成。

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In vitro conversion of S13 viral DNA in phage particles to the double-stranded DNA

The conversion of single-stranded DNA in S13 intact phage particles to the double-stranded replicative form DNA was observed in cell extracts prepared from Escherichia coli H560 (S13^S, polA, endA) cells lysed with lysozyme and the non-ionic detergent, Brij 58. The DNA product, which associated with a rapidly sedimenting component, was identified as RFII-DNA with a gap by sedimentation analysis. The conversion was inhibited by $N- ethylmaleimide$ , but not by rifampicin, nicotinamide mononucleotide or polymyxin B. The dnaB gene product was involved in the replicative system. Similar extracts prepared from a S13-resistant E. coli strain K12W6 also catalyzed this synthesis.

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Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis

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