Chinese Journal of Integrative Medicine最新文献

{"title":"Complex Intervention Frameworks for Acupuncture: Recommendations from a Scoping Review.","authors":"Zi-Yan Guo, Xue-Zhou Wang, Xiang-Yu Hu, Xiao-Yi Hu, Zi-Yu Tian, Huan Chen, Xiang-Hong Jing, Wei-Juan Gang","doi":"10.1007/s11655-026-4179-8","DOIUrl":"https://doi.org/10.1007/s11655-026-4179-8","url":null,"abstract":"<p><strong>Objective: </strong>To explore phase structures and evaluation components of existing complex intervention frameworks and to propose recommendations for future acupuncture frameworks.</p><p><strong>Methods: </strong>Six databases and multiple sources of gray literature were searched. Literature on complex interventions, including guidelines, frameworks, models, methodological studies, consensus statements, policy documents, and web-based guidance, were included. A narrative synthesis was conducted to examine phase settings and inter-phase relationships. The included frameworks were further assessed for their applicability to acupuncture evaluation, and preliminary recommendations were developed.</p><p><strong>Results: </strong>A total of 46 publications were included, identifying 21 eligible frameworks, which were further categorized into 14 framework series. The included frameworks were mainly characterized by 3 phases, including preparatory, intervention evaluation, and long-term evaluation. Two evaluation approaches were identified, clinical-effect-first and mechanistic-first. Two structural design logics, sequential linear and sequential iterative models, were observed. The most frequently addressed phase components were overall effect evaluation (n=10, 71.4%) and component evaluation (n=13, 92.9%), whereas long-term outcomes were rarely considered (n=3, 21.4%). Regarding applicability to acupuncture, 4 major aspects were identified: acupuncture theory integration, individualized treatment adjustment, long-term follow-up, and real-world heterogeneity.</p><p><strong>Conclusions: </strong>Existing frameworks vary substantially in phase structures and are not fully adapted to acupuncture. Potential considerations in future framework development include acupuncture theory and clinical experience, individualized intervention protocol, practitioner factors, acupuncture-specific outcomes, long-term follow-up and safety, and biomedical outcomes.</p>","PeriodicalId":10005,"journal":{"name":"Chinese Journal of Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148982478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zhangyanming Tablets for Dry Eye Disease: A Randomized, Double-Blind, Placebo-Controlled, Multicenter Clinical Study.","authors":"Hu-Ping Wu, Jian-Jun Gu, Jie Fang, Dong-Qing Zhao, Sheng-Tao Sun, Shun-Rong Luo, Zhi-Rong Lin, Jia-Jia Hu","doi":"10.1007/s11655-026-4050-y","DOIUrl":"https://doi.org/10.1007/s11655-026-4050-y","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the efficacy and safety of Zhangyanming Tablets (ZYMT) combined with 0.1% sodium hyaluronate eye drops for dry eye disease (DED).</p><p><strong>Methods: </strong>A stratified, randomized, doubleblind, placebo-controlled multicenter trial was conducted from June 2022 to August 2024, enrolling 144 patients with DED from 3 centers. Participants were randomized 1:1 to either the ZYMT group (ZYMT + 0.1% sodium hyaluronate, n=72) or the control group (placebo + 0.1% sodium hyaluronate, n=72). Participants received the study tablets (2 tablets orally, 3 times daily) concurrently with the eye drops (1 drop, 5 times daily) for 8 weeks. The primary outcome was the mean change from baseline in the ocular surface disease index (OSDI) score. The secondary outcomes included the efficacy rate, the mean change from baseline in the tear film breakup time (TFBUT), Schirmer's test, corneal fluorescein staining (CFS) and Chinese medicine (CM) syndrome score. Safety was assessed by monitoring adverse events, vital signs, and laboratory parameters (hematology, urinalysis, liver and kidney functions, and electrocardiogram).</p><p><strong>Results: </strong>A total of 119 patients completed this study, among which 58 were in the ZYMT group and 61 were in the control group. After 8 weeks of treatment, the mean change from baseline in the OSDI score in the ZYMT group was significantly greater than those in the control group in week 8 [absolute difference -5.66, 95% confidence interval (CI), -9.56 to -1.76]. The efficacy rate was 62.1% (36/58) in the ZYMT group versus 42.6% (26/61) in the control group (relative risk: 1.46, 95% CI, 1.02 to 2.08). The mean changes from baseline in TFBUT and CM symptom score were significantly greater in the ZYMT group than those in the control group at week 8 (P<0.05). No significant differences were observed between the two groups in Schirmer's test, CFS, or safety data (all P>0.05).</p><p><strong>Conclusion: </strong>ZYMT combined with 0.1% sodium hyaluronate eye drops can significantly alleviate the ocular symptoms, improve tear film, and demonstrate a good safety. (Registration No. ChiCTR2200061479).</p>","PeriodicalId":10005,"journal":{"name":"Chinese Journal of Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148954867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Shexiang Baoxin Pill (MUSKARDIA) on Cardiovascular Outcomes by Baseline Hemoglobin Levels in 2,494 Patients with Chronic Stable Coronary Artery Disease: A Secondary Subgroup Analysis.","authors":"Jing-Min Zhou, Hai-Ming Shi, Yang Wu, Yuan-Zhe Jin, Sheng-Huang Wang, Jun Qian, Chao-Xin Pan, Feng Lu, Xin-Li Li, Rong Li, Keng Wu, Liu-Yi Wang, Hong-Liang Cong, Zhi-Ming Yang, Yu-Ming Hao, Peng-Lin Yang, Yan-Qiu Chen, Jun-Bo Ge","doi":"10.1007/s11655-026-4048-5","DOIUrl":"https://doi.org/10.1007/s11655-026-4048-5","url":null,"abstract":"<p><strong>Objective: </strong>Shexiang Baoxin Pill (MUSKARDIA) reduces major adverse cardiovascular events (MACE) and improves angina, this study aims to evaluate its differential efficacy across varying hemoglobin (Hb) levels.</p><p><strong>Methods: </strong>This was a subgroup analysis of a multicenter, randomized, double-blind, placebo-controlled phase W trial. Patients with coronary artery disease (CAD) were stratified into two groups based on baseline Hb levels: Group A (Hb < 140 g/L, n=1,286) and Group B (Hb>140 g/L, n=1,208). The primary endpoint was MACE; secondary endpoints included non-fatal myocardial infarction (MI), non-fatal stroke, and all-cause mortality. Hazard ratios (HR) with 95% confidence intervals (CI) were estimated using Cox proportional hazards models.</p><p><strong>Results: </strong>In Group A, MUSKARDIA significantly reduced MACE risk (HR=0.45, 95% CI: 0.21-0.96, P=0.038), with a 68% reduction in non-fatal MI risk (P=0.036). Non-fatal stroke and all-cause mortality showed no significant differences. Adverse events were lower in the MUSKARDIA group (15.9% vs. 17.6%). In Group B, no significant MACE benefit was observed (P=0.354), with a trend toward increased non-fatal MI risk (HR=1.88). Non-fatal stroke and all-cause mortality were similar between groups. Adverse events were slightly higher (21.2% vs. 18.3%).</p><p><strong>Conclusions: </strong>MUSKARDIA demonstrated significant cardiovascular protective effects in CAD patients with Hb < 140 g/L, particularly reducing non-fatal MI risk, while showing limited efficacy in patients with Hb>140 g/L. This highlights its cardiovascular protective role in high-risk populations characterized by greater ischemic risk and inadequate myocardial oxygen supply.</p>","PeriodicalId":10005,"journal":{"name":"Chinese Journal of Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148954857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Holistic Regulation of Body-Mind Acupuncture Activates Leptin Receptor-AMPK Signaling to Restore Mitochondrial Homeostasis in Chronic Fatigue Syndrome in vitro and in vivo.","authors":"Jin-Xia Li, Bing Ji, Jing-Jun Xie, Xiao-Qing Guo, Zhong-Qiang Pan, Rui-Yang Fu, Xiao-Yin Lu, Hao-Yu Wu, Cong-Jian Jin","doi":"10.1007/s11655-026-3849-x","DOIUrl":"https://doi.org/10.1007/s11655-026-3849-x","url":null,"abstract":"<p><strong>Objective: </strong>To elucidate the therapeutic mechanism of holistic regulation of body and mind (HRBM) acupuncture in chronic fatigue syndrome (CFS) by focusing on leptin receptor (LepR)-AMP-activated protein kinase (AMPK) signaling to address mitochondrial dysfunction and oxidative stress.</p><p><strong>Methods: </strong>A CFS rat model was established by a 4-week treadmill-based exhaustive exercise protocol. After modeling, 50 rats were randomly allocated into 5 groups (n=10), control, CFS (model), acupuncture (Acu), Acu + Allo-aca, and Acu + Allo-aca + 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR) groups. Rats in the Acu group received acupuncture at bilateral Baihui (GV 20) and Zusanli (ST 36) for 30 min daily over 4 weeks. Behavioral assessments were used to confirm model validity and treatment effects. Skeletal muscle transcriptomics were applied to identify differentially expressed genes associated with mitochondrial metabolism. LepR, AMPK, phosphorylated AMPK (p-AMPK), peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α), and nuclear factor erythroid 2-related factor 2 (Nrf2) were validated by RT-qPCR and Western blot analyses. In vitro, chronic fatigue-like cells were treated with EX-527 (1 µmol/L) and AICAR (500 µmol/L). Mitochondrial membrane potential was assessed using JC-1 assay, intracellular ATP and reactive oxygen species (ROS) levels were quantified, and expressions of apoptosis-related proteins, antioxidative enzymes, and mitophagy-associated proteins were evaluated by Western blot analysis. Mitochondrial ultrastructure was observed by transmission electron microscopy. Immunofluorescence and DCFH-DA probes were used to assess the LepR/AMPK co-localization and ROS dynamics.</p><p><strong>Results: </strong>Acupuncture at GV 20 and ST 36 significantly improved fatigue-related behaviors and exercise endurance in CFS rats. Compared with the control group, rats with CFS exhibited markedly aggravated fatigue symptoms (P<0.01), whereas acupuncture treatment significantly ameliorated these deficits (P=0.0015). Transcriptomic analysis revealed that acupuncture activated LepR-AMPK signaling pathway, accompanied by enhanced mitochondrial function, increased ATP production, and improved antioxidative capacity. Furthermore, acupuncture promoted PINK1/Parkin-mediated mitophagy and stabilized mitochondrial ultrastructure. In vitro, treatment with EX-527 and/or AICAR improved mitochondrial and metabolic measures in the chronic fatigue-like cell model.</p><p><strong>Conclusions: </strong>HRBM acupuncture exerts therapeutic effects in CFS by activating LepR-AMPK signaling, thereby restoring mitochondrial bioenergetics, enhancing antioxidant defenses, and promoting mitophagy.</p>","PeriodicalId":10005,"journal":{"name":"Chinese Journal of Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148929189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic Insights into Acupuncture Mechanisms in Protecting Ovarian Function in Mice with Premature Ovarian Insufficiency.","authors":"Yu Luo, Meng Yu, Shi-Yu Feng, Su-Ning Huang, Hai-Chuan Xie, Bei Zhang, Yan Ning, Jia-Man Wu","doi":"10.1007/s11655-026-4249-y","DOIUrl":"https://doi.org/10.1007/s11655-026-4249-y","url":null,"abstract":"<p><strong>Objective: </strong>To explore the molecular mechanisms underlying the protective effect of acupuncture on ovarian function in mice with cyclophosphamide-induced premature ovarian insufficiency (POI) via transcriptomic analysis.</p><p><strong>Methods: </strong>Twenty female C57BL/6 mice were divided into 4 groups: control, model, acupuncture, and non-meridian/non-acupoint (NOMA). POI was induced in the model, acupuncture, and non-meridian/non-acupoint groups via cyclophosphamide injection. The acupuncture group received acupuncture at Guanyuan (CV 4), bilateral Guilai (ST 29), and Sanyinjiao (SP 6) for 3 weeks. After the intervention, ovarian tissue weight and ovarian coefficient were calculated, serum levels of key reproductive hormones including follicle-stimulating hormone (FSH), luteinizing hormone (LH) and anti-Müllerian hormone (AMH) were detected, and ovarian histopathological changes were observed to evaluate ovarian function. Transcriptome sequencing was performed to identify differentially expressed genes (DEGs), followed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses to explore key functional terms and signaling pathways. Western blot was finally applied to validate the expression of core proteins related to mitochondrial function, endoplasmic reticulum stress and inflammatory pathways.</p><p><strong>Results: </strong>The model group showed reduced ovarian weight and elevated FSH levels. The acupuncture group exhibited significantly higher ovarian weight and coefficient, lower FSH levels, and increased E₂ and AMH levels compared to the model group (all P<0.01). Transcriptomic analysis revealed 4,021 DEGs between groups. GO and KEGG analyses revealed that these DEGs were mainly involved in oocyte development, steroid hormone synthesis, and pathways related to mitochondrial function, endoplasmic reticulum stress, and inflammatory signaling. Western blot analysis showed that acupuncture partially restored mitochondrial function markers cytochrome c oxidase subunit IV and NADH dehydrogenase 1 beta subcomplex subunit 8 and reduced endoplasmic reticulum stress markers (glucose-regulated protein 78 and Calnexin, P<0.01). It also downregulated pro-inflammatory proteins (IL-17R, IL-17A, NF-κB p65, p-NF-κB p65, ERK1/2, and p-ERK1/2) and upregulated proteins related to metabolic homeostasis (peroxisome proliferator-activated receptor γ, receptor-interacting protein 140, nicotinamide phosphoribosyltransferase, and sirtuin 1, P<0.01).</p><p><strong>Conclusion: </strong>Acupuncture effectively alleviates cyclophosphamide-induced POI in mice, improves ovarian function and follicular quality by regulating cellular functions and inflammatory pathways, suggesting a novel therapeutic approach for POI.</p>","PeriodicalId":10005,"journal":{"name":"Chinese Journal of Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148918493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of Chinese Medicine in Treating Pediatric Respiratory Infections: A Comprehensive Review.","authors":"Chao-Hong Zhang, Guo-Bin Lin, Xiao-Fei Lu, Xi Yu","doi":"10.1007/s11655-026-3963-9","DOIUrl":"https://doi.org/10.1007/s11655-026-3963-9","url":null,"abstract":"","PeriodicalId":10005,"journal":{"name":"Chinese Journal of Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148929111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Paradigm Evolution of Rome Criteria over 36 Years and Integration of Chinese and Western Medicine: From \"Functional Gastrointestinal Disorders\" to \"Disorders of Gut-Brain Interaction\".","authors":"Pei-Hua Shen, Xin-Xin Hu, Tian-Yuan Jiang, Wei Wei","doi":"10.1007/s11655-026-3870-0","DOIUrl":"https://doi.org/10.1007/s11655-026-3870-0","url":null,"abstract":"","PeriodicalId":10005,"journal":{"name":"Chinese Journal of Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148879344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Babao Dan Improves Doxorubicin-Induced Cardiomyopathy by Activating Nrf2/GPX4 Signaling Pathway.","authors":"Xian-Mei Li, Jiu-Mao Lin, Lan-Xin Yu, Xue-Jiao Wang, Lai-Feng Zeng, Feng-Hua Zhang","doi":"10.1007/s11655-026-4044-9","DOIUrl":"https://doi.org/10.1007/s11655-026-4044-9","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the protective effects and underlying mechanisms of Babao Dan (BBD) against doxorubicin (Dox)-induced cardiomyopathy (DIC).</p><p><strong>Methods: </strong>A zebrafish model of DIC alongside an in vitro model of cardiomyocyte injury induced by Dox was utilized to elucidate the protective effects of BBD against DIC. The zebrafish and cell models were divided into control, Dox, and BBD (low-, medium-, and high-dose) plus Dox treatment groups. Reactive oxygen species (ROS) and malondialdehyde (MDA) content were determined by commercial kits. Nrf2/GPX4 signaling activity was assessed by Western blot and RT-qPCR. A comprehensive network pharmacology analysis was conducted to explore the potential mechanisms of action of BBD on DIC.</p><p><strong>Results: </strong>Compared with Dox group, BBD treatment significantly reduced ROS levels and decreased MDA content in zebrafish (P<0.01), indicating ameliorated oxidative stress, which contributed to a reduction in Dox-induced cardiac injury. Notably, BBD was found to inhibit cardiomyocyte ferroptosis by restoring mitochondrial function in vitro. Network pharmacology analysis identified 4 core network genes, including Nrf2, which is pivotal in linking oxidative stress with ferroptosis, along with 6 key active components of BBD. Molecular docking and dynamics simulations demonstrated that these active components exhibit a strong binding affinity with the identified core targets.</p><p><strong>Conclusions: </strong>BBD effectively activates the Nrf2/GPX4 signaling pathway, thereby inhibiting cardiomyocyte ferroptosis and mitigating DIC. These insights provide a theoretical foundation for the future exploration of BBD as a preventive and therapeutic agent against Dox-related cardiotoxicity.</p>","PeriodicalId":10005,"journal":{"name":"Chinese Journal of Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148879328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Danggui Sini Decoction Alleviates Sciatica by Reducing Peripheral Sensitization through Downregulation of Transient Receptor Potential Channels in Dorsal Root Ganglions.","authors":"Di Zhang, Zi-Feng Zhuang, Shao-Zi Lin, Zen-Ni Chen, Hong-Ling Chen, Jun-Yan Gu, Yu-Ting Hao, Yi-Xuan Li, Jia-Xu Chen, Guo-Ping Zhao","doi":"10.1007/s11655-026-4045-8","DOIUrl":"https://doi.org/10.1007/s11655-026-4045-8","url":null,"abstract":"<p><strong>Objective: </strong>To elucidate whether Danggui Sini Decoction (DGSD) relieves sciatica by downregulating transient receptor potential (TRP) channels involved in peripheral sensitization.</p><p><strong>Methods: </strong>After conducting chronic constriction injury of sciatic nerve (CCI) operation, 32 SD rats, randomly assigned to the sham, CCI 7d, CCI 14d, and CCI 21d groups (n=8 per group), were used to observe the course of sciatica. mRNA sequencing of dorsal root ganglia (DRG) was conducted on rats in the sham and CCI 21d groups. Another 32 rats were divided into the sham, CCI, mecobalamin, and DGSD groups, 8 in each group. mRNA sequencing analysis and single-cell RNA-sequencing (scRNA-seq) data analysis were conducted. Behavioural tests were conducted to evaluate the therapeutic effects of DGSD. The levels of TRP vanilloid (TRPV)1-4, TRP ankyrin 1 (TRPA1), TRP melastatin 8 (TRPM8), Ras homolog family member A (RhoA), and phosphorylated p38 mitogen-activated protein kinase (p-p38MAPK) were measured via Western blot, and the serum levels of C-C motif chemokine ligand 2 and 5 (CCL2, CCL5), substance P (SP), and prostaglandin E2 (PGE2) were conducted via ELISA.</p><p><strong>Results: </strong>scRNA-seq and mRNA sequencing analysis revealed that inflammatory mediator regulation of TRP channels played a role in the development of sciatica. CCI rats exhibited cold hyperalgesia from day 4 to 21, increased the DRG protein expression levels of TRP channels, including TRPV1-4, TRPA1, TRPM8, RhoA, p-p38MAPK, CCL2, CCL5, SP, and PGE2. Treatment with DGSD decreased the serum levels of CCL2, CCL5, SP, and PGE2, and reduced the levels of RhoA, p-p38MAPK, TRPV1-4, TRPA1, and TRPM8 in DRG (P<0.05).</p><p><strong>Conclusion: </strong>CCI increased the levels of TRP channels and inflammatory factors, inducing cold hyperalgesia, whereas DGSD relieved pain via inhibiting peripheral sensitization to relieve cold hyperalgesia by reducing the inflammatory factors and TRP.</p>","PeriodicalId":10005,"journal":{"name":"Chinese Journal of Integrative Medicine","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148879347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dendrobium officinale Polysaccharides Protected against CCl₄-Induced Liver Fibrosis by Inhibiting Oxidative Stress and TGF-β1 and Wnt/β-Catenin Signaling.","authors":"Qin Fan, Liu-Qing Yang, Xian-Xian Tian, Ying Xu, Ji-Na Luo, Xu-Wei Liu, Lan-Hua Chen, Sen Kou, Yu-Ming Shi, Ya-Jie Bai, Qing-Fang Ji, Yuan Long, Jia-Mao Cheng, Hai-Yan Chen","doi":"10.1007/s11655-025-3930-x","DOIUrl":"10.1007/s11655-025-3930-x","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the potential anti-liver fibrosis (LF) effects of Dendrobium officinale polysaccharides (DOPs) and to further explore the involvement of the antioxidant and anti-inflammatory mechanisms of the transforming growth factor-beta 1 (TGF-β1) and Wnt signaling pathways.</p><p><strong>Methods: </strong>Seventy male Sprague-Dawley rats were assigned to 5 groups in a randomized block design: control, model, colchicine (COL, 0.1 mg/kg), low-dose DOP (LDD, 0.05 g/kg), and high-dose DOP (HDD, 0.1 g/kg) groups (n=14 per group). LF rat model was induced via subcutaneous injection of carbon tetrachloride (CCl₄). Serum markers of oxidative stress, liver function, and LF were monitored. Pathological examination of liver tissue was performed using hematoxylin and eosin (HE) and Masson's trichrome staining, followed by staging evaluation using the meta-analysis of histological data in viral hepatitis scoring system. Quantitative analysis of the core components of extracellular matrix (ECM) and the regulatory factors [e.g., tissue inhibitor of metalloproteinase-1 (TIMP-1), matrix metalloproteinase-1 (MMP-1)], TGF-β1, and Wnt/β-catenin signaling pathway were performed using real-time quantitative polymerase chain reaction and Western blot, respectively. In addition, the therapeutic effect of DOPs on LF was further evaluated using acoustic radiation force pulses (AFRI) and contrast-enhanced ultrasound (CEUS).</p><p><strong>Results: </strong>Compared with the model group, HDD dramatically improved liver appearance and index, and alleviated extensive hepatocyte degeneration, interstitial fibrous proliferation, and pseudolobuli formation (P<0.01). Moreover, HDD downregulated mRNA and protein levels of α-smooth muscle actin, type I collagen, and type III collagen in the liver tissue of rats, while maintaining the MMP-1/TIMP-1 ratio by upregulating MMP-1 and downregulating TIMP-1 (P<0.05 or P<0.01). The ARFI and CEUS examination results also confirmed that HDD had significant anti-LF effects. HDD inhibited activation of the TGF-β and Wnt/β-catenin signaling pathways by reducing mRNA and protein levels of TGF-β1, Wnt-1 and β-catenin, while increasing the expression of dickkopf-1 (P<0.05 or P<0.01).</p><p><strong>Conclusions: </strong>DOPs achieved an anti-LF effect by inhibiting TGF-β1 and Wnt/β-catenin signaling. ARFI and CEUS can be used to evaluate the liver stiffness, microcirculation disorders, and drug efficacy in LF and cirrhosis.</p>","PeriodicalId":10005,"journal":{"name":"Chinese Journal of Integrative Medicine","volume":" ","pages":"817-828"},"PeriodicalIF":3.1,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145328461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}