Photochemical & Photobiological Sciences最新文献_第7页

Cyanine dye-embedded fluorescent film for ratiometric pH measurement. 比例pH测量用菁染料包埋荧光膜。

IF 2.7 3区化学

Photochemical & Photobiological Sciences Pub Date : 2025-01-01 Epub Date: 2025-01-21 DOI: 10.1007/s43630-025-00681-3

Roch Sobczyk, Laurent Galmiche, Cédric Mongin, Meriem Djendli, Isabelle Leray, Rachel Méallet

{"title":"Cyanine dye-embedded fluorescent film for ratiometric pH measurement.","authors":"Roch Sobczyk, Laurent Galmiche, Cédric Mongin, Meriem Djendli, Isabelle Leray, Rachel Méallet","doi":"10.1007/s43630-025-00681-3","DOIUrl":"10.1007/s43630-025-00681-3","url":null,"abstract":"The precise monitoring of pH is critical in various applications, particularly in biology-related areas. In this work, we report the synthesis and characterization of a novel cyanine-based fluorescent pH sensor with a pKa around 6. This pH-sensitive dye features a cyanine chromophore coupled to a piperazine moiety, which modulates the protonation equilibrium and thus the optical response. DTF calculation corroborates the changes in the photophysical properties upon protonation. Upon immobilization within a hydrophilic polymer matrix, the resulting fluorescent sensor is exhibited a pKa around 4.5, facilitating ratiometric pH measurements for acidic media (for instance in food-examples given in milk and fresh yogurt). Importantly, this probe design allowed a less than 5 min response time to pH changes while avoiding cytotoxic effects towards E. coli. The development of such a fluorescent pH indicator is motivated by the growing need for optical monitoring tools capable of providing real-time, non-invasive readouts of pH dynamics in complex media and microfluidic systems.","PeriodicalId":98,"journal":{"name":"Photochemical & Photobiological Sciences","volume":" ","pages":"165-179"},"PeriodicalIF":2.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142996581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chiroptical response of an array of isotropic plasmonic particles having a chiral arrangement under coherent interaction. 具有手性排列的各向同性等离子体粒子阵列在相干相互作用下的涡旋响应。

IF 2.7 3区化学

Photochemical & Photobiological Sciences Pub Date : 2025-01-01 Epub Date: 2024-12-10 DOI: 10.1007/s43630-024-00667-7

Tomoya Oshikiri, Yasutaka Matsuo, Hiromasa Niinomi, Masaru Nakagawa

{"title":"Chiroptical response of an array of isotropic plasmonic particles having a chiral arrangement under coherent interaction.","authors":"Tomoya Oshikiri, Yasutaka Matsuo, Hiromasa Niinomi, Masaru Nakagawa","doi":"10.1007/s43630-024-00667-7","DOIUrl":"10.1007/s43630-024-00667-7","url":null,"abstract":"The chirality and chiroptical response of materials have attracted significant attention for their potential to introduce the new science of light-matter interactions. We demonstrate that collective mode formation under modal coupling between localized surface plasmon resonances (LSPRs) with a chiral arrangement and Fabry-Pérot (FP) nanocavity modes can induce chiroptical responses. We fabricated a cluster of isotropic gold nanodisks with a chiral arrangement (gold nano-windmills, Au-NWs) on the FP nanocavities of TiO2 and Au film. The differential absorption of the Au-NWs coupled with the FP nanocavities under left- and right-handed circularly polarized light irradiations in the far field was significantly enhanced compared with the differential absorption without the FP nanocavities. Far- and near-field analyses by numerical simulation revealed that the Au-NWs coupled with the FP nanocavities formed a collective mode in the near field, and the collective mode represented the chiroptical response in the far field. The light field with the large helicity, can be used in chiral light-matter interactions. The concept of collective mode formation using isotropic metal nanodisks coupled with FP nanocavities provides a platform for controlling complex light fields.","PeriodicalId":98,"journal":{"name":"Photochemical & Photobiological Sciences","volume":" ","pages":"13-21"},"PeriodicalIF":2.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142798740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Balance between photoreduction efficiency, cofactor affinity, and allosteric coupling of halogenase flavoenzymes. 卤素酶黄酶的光还原效率、辅因子亲和力和变构偶联之间的平衡。

IF 2.7 3区化学

Photochemical & Photobiological Sciences Pub Date : 2025-01-01 Epub Date: 2024-12-30 DOI: 10.1007/s43630-024-00670-y

Niklas Diepold, Friederike Reese, Tina Prior, Christian Schnepel, Norbert Sewald, Tilman Kottke

{"title":"Balance between photoreduction efficiency, cofactor affinity, and allosteric coupling of halogenase flavoenzymes.","authors":"Niklas Diepold, Friederike Reese, Tina Prior, Christian Schnepel, Norbert Sewald, Tilman Kottke","doi":"10.1007/s43630-024-00670-y","DOIUrl":"10.1007/s43630-024-00670-y","url":null,"abstract":"Flavin-dependent halogenases (FDHs) are promising candidates for the sustainable production of halogenated organic molecules by biocatalysis. FDHs require only oxygen, halide and a fully reduced flavin adenine dinucleotide (FADH-) cofactor to generate the reactive HOX that diffuses 10 Å to the substrate binding pocket and enables regioselective oxidative halogenation. A key challenge for the application of FDHs is the regeneration of the FADH-. In vitro, FADH- can be regenerated by photoreduction of the oxidized FAD inside the protein using blue light, turning the halogenase into an inefficient artificial photoenzyme. We aimed to improve the photochemical properties of the tryptophan 5-halogenase PyrH from Streptomyces rugosporus by structure-guided mutagenesis. W279 and W281 of the conserved WxWxIP-motif close to FAD were exchanged against phenylalanine. Time-resolved UV-vis spectroscopy showed that the W281F exchange indeed increased the quantum yield of the one- and two-electron reduction, respectively. The cofactor binding affinity decreased slightly with dissociation constants rising from 31 to 74 μM, as examined by fluorescence anisotropy. FTIR difference spectroscopy demonstrated that the allosteric coupling between the FAD and substrate binding sites was mostly preserved. In contrast, the double mutant did not improve the yield further, while negatively affecting binding affinity and structural coupling. The distal W279F exchange was less effective in all parameters. Photoreductions were additionally delayed by a reversible inactive conformation. We conclude that there is a delicate balance to be considered for screening of FDHs for biocatalysis. Variant PyrH-W281F was found to be the most promising candidate for the application as artificial photoenzyme.","PeriodicalId":98,"journal":{"name":"Photochemical & Photobiological Sciences","volume":" ","pages":"37-51"},"PeriodicalIF":2.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142908831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Divulging the potency of naturally derived photosensitizers in green PDT: an inclusive review Of mechanisms, advantages, and future prospects. 揭示天然衍生光敏剂在绿色PDT中的效力：对机制，优势和未来前景的全面回顾。

IF 2.7 3区化学

Photochemical & Photobiological Sciences Pub Date : 2025-01-01 Epub Date: 2024-12-10 DOI: 10.1007/s43630-024-00669-5

Muskan Gupta, Anwesha Sahu, Tuhin Mukherjee, Satyajit Mohanty, Priyamjeet Das, Nikita Nayak, Shivangi Kumari, Ravi Pratap Singh, Ashok Pattnaik

{"title":"Divulging the potency of naturally derived photosensitizers in green PDT: an inclusive review Of mechanisms, advantages, and future prospects.","authors":"Muskan Gupta, Anwesha Sahu, Tuhin Mukherjee, Satyajit Mohanty, Priyamjeet Das, Nikita Nayak, Shivangi Kumari, Ravi Pratap Singh, Ashok Pattnaik","doi":"10.1007/s43630-024-00669-5","DOIUrl":"10.1007/s43630-024-00669-5","url":null,"abstract":"Photodynamic Therapy (PDT) offers a minimally invasive approach for treating various health conditions, employing a photosensitizer (PS) and specific light. Recent enhancements make PDT outpatient-friendly and less discomforting. Effectiveness hinges on selecting the appropriate PS. This article delves into natural and synthetic PSs, emphasizing the rising interest in natural alternatives for their safety. It explores their mechanisms, characteristics, and applications, offering insights into their potential contributions to advancing PDT. This extensive review delves into the preclinical and clinical landscape of natural PSs for PDT, shedding light on their diverse applications and promising outcomes. Compounds like curcumin, piperine, riboflavin, psoralen, hypericin, and others show significant potential in preclinical in vitro studies across various cell lines. In vivo, these photosensitizers prove effective against skin tumors, carcinomas, and sarcomas, inducing apoptosis, autophagy, and ROS generation for therapeutic efficacy. The review underscores the critical role of proper dosing and monitoring in balancing therapeutic benefits and risks. It highlights the advantages and limitations of natural PSs, emphasizing their specific targeting, bioavailability, and limited side effects. The future of PDT holds promising breakthroughs, taking from some evidence like Bergamot oil in nanostructured lipid carriers for dermatological conditions. Second-generation photosensitizer Tookad shows potential in prostate cancer treatment, while Tripterygium wilfordii Hook. F. emerges as an antimicrobial PDT source etc. Thus, environmental concerns in PDT prompt a shift to plant extracts for PS purification. The evidence-supported focus on natural PSs establishes this article as a key resource for advancing natural compounds in PDT and their therapeutic applications.","PeriodicalId":98,"journal":{"name":"Photochemical & Photobiological Sciences","volume":" ","pages":"191-214"},"PeriodicalIF":2.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142798744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Twenty-first century surface UV radiation changes deduced from CMIP6 models. Part I: evolution of major influencing factors. 更正：根据CMIP6模式推断的21世纪地表紫外线辐射变化。第一部分：主要影响因素的演变。

IF 2.7 3区化学

Photochemical & Photobiological Sciences Pub Date : 2025-01-01 DOI: 10.1007/s43630-025-00685-z

A Chatzopoulou, K Tourpali, A F Bais, P Braesicke

引用次数: 0

Type I and Type II photosensitization of DNA etheno adducts. DNA乙二烯加合物的I型和II型光敏性。

IF 2.7 3区化学

Photochemical & Photobiological Sciences Pub Date : 2025-01-01 Epub Date: 2024-12-19 DOI: 10.1007/s43630-024-00663-x

Paloma Lizondo-Aranda, Gemma M Rodríguez-Muñiz, Miguel A Miranda, Belinda Heyne, Virginie Lhiaubet-Vallet

引用次数: 0

Unravelling the modes of phototoxicity of NIR absorbing chlorophyll derivative in cancer cells under normoxic and hypoxic conditions. 揭示近红外吸收叶绿素衍生物在常氧和缺氧条件下对癌细胞的光毒性模式。

IF 2.7 3区化学

Photochemical & Photobiological Sciences Pub Date : 2025-01-01 Epub Date: 2025-01-18 DOI: 10.1007/s43630-024-00680-w

Sucharita Chatterjee, Alok Dube, Shovan Kumar Majumder

{"title":"Unravelling the modes of phototoxicity of NIR absorbing chlorophyll derivative in cancer cells under normoxic and hypoxic conditions.","authors":"Sucharita Chatterjee, Alok Dube, Shovan Kumar Majumder","doi":"10.1007/s43630-024-00680-w","DOIUrl":"10.1007/s43630-024-00680-w","url":null,"abstract":"The efficacy of photodynamic treatment (PDT) against deep-seated tumor is hindered by low penetration depth of light as well as hypoxic conditions which prevails in tumor. To overcome this limitation, Near-infrared (NIR) absorbing photosensitizers have been investigated actively. In the present study we evaluated the PDT efficacy of an NIR absorbing chlorophyll derivative 'Cycloimide Purpurin-18 (CIPp-18)' in Human Breast carcinoma (MCF-7) and cervical adenocarcinoma (Hela) cells under normoxic and hypoxic conditions. PDT with CIPp-18 (2.0 µM, 3 h) and NIR light (700 ± 25 nm, 0.36-1.4 J /cm2) induced potent phototoxicity in both the cell lines. Under hypoxic conditions, PDT induced ~ 32% and 42% phototoxicity at LD50 and LD70 light dose, respectively, which corresponds to phototoxic dose under normoxia. CIPp-18 in neat buffer (pH 7.4) showed generation of singlet oxygen (1O2) as well as superoxide (O2·-) radicals. Studies on ROS generation in cells using fluorescence probes and the effect of mechanistic probes of 1O2 (Sodium Azide, Histidine, D2O) and free radicals (DMSO, Mannitol, Cyanocobalamin, SOD-PEG) on phototoxicity show that 1O2 plays major role in phototoxicity under normoxia. Whereas, under hypoxic conditions, PDT led to no significant generation of ROS and phototoxicity remained unaffected by cyanocobalamin, a quencher of O2·-. Moreover, CIPp-18 showed localization in cell membrane and PDT led to more pronounced loss of membrane permeability in cells under hypoxia than for normoxia. These results demonstrate that CIPp-18 is suitable for PDT of cancer cells under hypoxia and also suggest that phototoxicity under hypoxia is mediated via ROS-independent contact-dependent mechanism.","PeriodicalId":98,"journal":{"name":"Photochemical & Photobiological Sciences","volume":" ","pages":"149-164"},"PeriodicalIF":2.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142996582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibacterial effect of new photocatalytically active "complex oxides/PMMA" composites under visible-light irradiation. 新型光催化活性“复合氧化物/PMMA”复合材料在可见光照射下的抗菌效果。

IF 2.7 3区化学

Photochemical & Photobiological Sciences Pub Date : 2024-12-01 Epub Date: 2024-11-29 DOI: 10.1007/s43630-024-00664-w

Andrey Yu Shishkin, Diana G Fukina, Victoria O Rumyantseva, Elena V Shilova, Alexander S Ganov, Irina A Shalaginova, Pavel V Kornienko, Evgeny V Suleimanov, Ludmila L Semenycheva, Vasily F Smirnov

{"title":"Antibacterial effect of new photocatalytically active \"complex oxides/PMMA\" composites under visible-light irradiation.","authors":"Andrey Yu Shishkin, Diana G Fukina, Victoria O Rumyantseva, Elena V Shilova, Alexander S Ganov, Irina A Shalaginova, Pavel V Kornienko, Evgeny V Suleimanov, Ludmila L Semenycheva, Vasily F Smirnov","doi":"10.1007/s43630-024-00664-w","DOIUrl":"10.1007/s43630-024-00664-w","url":null,"abstract":"New photocatalytic materials based on complex oxides and a widely used and cheap polymer (PMMA) have been prepared. Among complex oxides previously investigated, the following have been used-RbTe1.5W0.5O6, CsTeMoO6, CsV0.625Te1.375O6, NaVMoO6, KVMoO6. For comparison, the binary oxides TiO2 and WO3 were used. The form of PMMA matrix was used as sponge and glass. The amount of powder in PMMA was selected based on retaining the polymer properties and getting the photocatalytic activity, which corresponds to 1% powder in sponge and 0.5% in glass. The \"photocatalyst/PMMA\" composites decompose the methylene blue under visible and UV light as well as possess antibacterial properties. The high electron-hole recombination was found out for composites photocatalysts, which significantly influences only on organic compounds decomposition in solutions. However, there is no direct dependence between effective photodegradation of simple organic molecules and antimicrobial properties. Inactivation of bacteria is determined by many factors such as active generated radicals, adsorption properties of the surface and the photocatalyst form, which can change the main active radicals. The comparison of photocatalytic action on organic solutions and bacteria of initial powders and PMMA composites allow choosing the most effective combination for further application. The most promising antimicrobial properties for composites have been obtained using compounds with β-pyrochlore structure.","PeriodicalId":98,"journal":{"name":"Photochemical & Photobiological Sciences","volume":" ","pages":"2237-2253"},"PeriodicalIF":2.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142749432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Self-aggregation of 13²,13²-disubstituted bacteriochlorophyll-d analog. 132,132-二取代细菌叶绿素-d 类似物的自聚集。

IF 2.7 3区化学

Photochemical & Photobiological Sciences Pub Date : 2024-12-01 Epub Date: 2024-11-27 DOI: 10.1007/s43630-024-00662-y

Yamato Hashimoto, Toyoho Takeda, Shin Ogasawara, Hitoshi Tamiaki

引用次数: 0

On the application of nanosecond laser flash photolysis to the investigation of the photoreactivity of di and triarylamines. 纳秒激光闪光光解在二胺和三芳胺光反应性研究中的应用。

IF 2.7 3区化学

Photochemical & Photobiological Sciences Pub Date : 2024-12-01 Epub Date: 2024-12-05 DOI: 10.1007/s43630-024-00661-z

Sergio M Bonesi, Stefano Protti

引用次数: 0