IF 2.7 3区 化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Paloma Lizondo-Aranda, Gemma M Rodríguez-Muñiz, Miguel A Miranda, Belinda Heyne, Virginie Lhiaubet-Vallet
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引用次数: 0

摘要

研究人员进行了光物理和光化学研究,以检验 1,N6-乙烯腺嘌呤(εdA)和 1,N2-乙烯鸟嘌呤(εdG)这两种乙烯加合物在两种著名的光敏剂(分别为 4-羧基二苯甲酮(CBP)和玫瑰红(RB))作用下的光反应活性。稳态光解实验结合高效液相色谱法(HPLC)和质谱测量法得出了与修复的核苷相对应的光反应产物。为了确定这种光氧化过程的机理,研究人员进行了磷光光谱分析、直接检测单线态氧发光和激光闪烁光解。这项工作证实,εdG 和 εdA 对两种类型的过程(I 型和 II 型)都很敏感。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Type I and Type II photosensitization of DNA etheno adducts.

Photophysical and photochemical studies were carried out to examine the photoreactivity of etheno adducts, 1,N6-ethenoadenine (εdA) and 1,N2-ethenoguanine (εdG), in the presence of two well-known photosensitizers acting by Type I and/or Type II mechanisms such as 4-carboxybenzophenone (CBP) and rose Bengal (RB), respectively. Steady-state photolysis experiments combined with HPLC and mass spectroscopy measurements lead to photoproducts that correspond to the repaired nucleosides. To determine the mechanism of this photooxidation processes, phosphorescence spectroscopy, direct detection of singlet oxygen luminescence and laser flash photolysis were carried out. This work establishes that εdG and εdA are sensitive to both types of processes (Type I and II).

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来源期刊
Photochemical & Photobiological Sciences
Photochemical & Photobiological Sciences 生物-生化与分子生物学
CiteScore
5.60
自引率
6.50%
发文量
201
审稿时长
2.3 months
期刊介绍: A society-owned journal publishing high quality research on all aspects of photochemistry and photobiology.
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