Cereal Research Communications最新文献

{"title":"Genome-wide identification, expression profiling, and network analysis of calcium and cadmium transporters in rice (Oryza sativa L.)","authors":"Shubham Kothari, V. K. Sharma, Ashutosh Singh, Sumeet Kumar Singh, Sarita Kumari","doi":"10.1007/s42976-024-00492-9","DOIUrl":"https://doi.org/10.1007/s42976-024-00492-9","url":null,"abstract":"<p>Calcium (Ca) and cadmium (Cd) are transition metals coexisting in the ecosystem. Ca is indispensable for the growth and development of plants as well as animals, while Cd is regarded as a toxic heavy metal for the living system. The transportation of Cd in the biological systems often used the pathways of Ca because of chemical similarities. High concentrations of cadmium replace Ca, Mn, and Zn from their respective metalloprotein sites and strongly associated with them. Replaced minerals from their metalloprotein sites are often released as an oxidative ion that is detrimental to it. The common transportation mechanism of Ca and Cd is implicit in the role of common and similar transporters for transporting them in plants. Thus, our study was done to identify the transporters for Ca and Cd and characterize them for similarity in terms of cotransportation system. A profile-based search program identified 44 transporters genes for Ca transportation and 70 genes for cadmium transportation. They were categorized into different groups based on the presence of signature motifs and domains. Identified transporters were characterized for genomic distribution, gene structure, annotation, conserved signature motifs, and domain. Further, <i>cis</i> motif analysis, heat map, gene ontology, and protein–protein interaction were conducted for Ca and Cd transporter genes. In silico expression showed Os05g0319800-1304 and Os0319800-6065 transporter genes were overexpressed for Ca and Os07g00232800-40298 and Os07g00384500-25924 transporter genes overexpressed for Cd transporter. These genes could be used as a candidate genes for enhancing the Ca concentration with reduced Cd content in rice using biotechnological approaches. Twenty-seven genes were found as the common transporters for Ca and Cd. Both active and passive transporter mechanisms act as cotransporters for Ca and Cd. The common signature motifs and domains can be targeted for the characterization of cotransporters of different minerals.</p>","PeriodicalId":9841,"journal":{"name":"Cereal Research Communications","volume":"61 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139757384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Root architecture traits and genotypic responses of wheat at seedling stage to water-deficit stress","authors":"Afrasyab Rahnama, Bahare Hosseinalipour, Ahmad Farrokhian Firouzi, Matthew Tom Harrison, Mansour Ghorbanpour","doi":"10.1007/s42976-023-00481-4","DOIUrl":"https://doi.org/10.1007/s42976-023-00481-4","url":null,"abstract":"<p>This study was aimed to identify genotypic differences in shoot growth rate and root architecture traits in response to water-deficit stress at early growth stages of wheat seedling, and to quantify relationships between genotypic variation in shoot physiological traits and root system architecture with a drought tolerance at several development stages. Eight bread wheat cultivars, namely Bam, Mahooti, Roshan, Tabbasi, Atrak, Falat, Shiraz and Qods, were grown in polyvinyl chloride tubes filled with soil in the greenhouse under well-watered and water-deficit stress conditions. Water stress elicited genotypic variation in root traits and shoot growth across cultivars. Drought stress decreased root architecture traits, with greater effects in drought-sensitive cultivars compared with those that were drought-tolerant. Branch root length was less influenced compared with seminal root length. We showed that cultivars Roshan and Bam were most tolerant to drought due to their shorter distance between the first branch root and the root tip, higher branch root length, longer seminal roots and higher stomatal conductance compared to the other cultivars. Positive relationship between root growth and shoot physiological responses was quantified upon drought stress, highlighting the role of leveraging more efficient root systems as a strategy to enhance resource uptake under water-deficit conditions. Thus, root growth responses can be used as a drought tolerance selection criterion at the seedling stage of employed genotypes.</p>","PeriodicalId":9841,"journal":{"name":"Cereal Research Communications","volume":"91 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139757701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the role of green synthesized cerium nanoparticles in enhancing wheat's drought tolerance: a comprehensive study of biochemical parameters and gene expression","authors":"","doi":"10.1007/s42976-024-00493-8","DOIUrl":"https://doi.org/10.1007/s42976-024-00493-8","url":null,"abstract":"<h3>Abstract</h3> <p>One of the primary causes restricting wheat output is drought, which is turning into an increasingly serious issue in many of the world's wheat-growing countries. Through the investigation of biochemical parameters and gene expression analysis, the current study examined the impact of foliar application of green synthesized cerium nanoparticles (Ce NPs) in mitigating drought stress on wheat development. For the experimental investigation, two irrigation regimes (100% SMC and 50% SMC) and four distinct Ce NP concentrations (25 ppm, 50 ppm, 75 ppm, and 100 ppm) were employed. We found that applying Ce NPs topically increased wheat plants' overall capacity to withstand drought. Among the different concentrations used, the foliar application with 50-ppm Ce NPs substantially increased the antioxidant enzyme activity, proline content, relative water content, and all photosynthetic matrices under drought stress conditions, in comparison with the control. Ce NPs treatment also lowered the hydrogen peroxide (50.53%) and malondialdehyde amounts (22.79%) under drought stress. To substantiate the impact of Ce NPs at the molecular level, we also examined the gene expression of five stress-related genes (<em>MYB33, MYB3R,</em> <em>DREB2, ABC1,</em> and <em>SnRK2.4</em>) and observed their upregulated expression after Ce NPs treatment under drought stress conditions. These results demonstrate the use of Ce NPs as a new strategy to mitigate the detrimental effects of drought stress in wheat.</p> <span> <h3>Graphical abstract</h3> <p>Illustrating the role of Ce NPs in mitigating drought stress in plants through biochemical and molecular regulation (created in BioRenders.com)</p> <p> <span> <span> <img alt=\"\" src=\"https://static-content.springer.com/image/MediaObjects/42976_2024_493_Figa_HTML.png\"/> </span> </span></p> </span>","PeriodicalId":9841,"journal":{"name":"Cereal Research Communications","volume":"1 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139677695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of local finger millet (Eleusine coracana (L.) Gaertn.) landraces for salinity tolerance using growth and biochemical traits at the seedling stage","authors":"Sumaiya S. Shaikh, Nitin T. Gore, Ganesh D. Mankar, Rajkumar B. Barmukh, Pankaj S. Mundada, Suraj D. Umdale, Mahendra L. Ahire","doi":"10.1007/s42976-024-00491-w","DOIUrl":"https://doi.org/10.1007/s42976-024-00491-w","url":null,"abstract":"<p>Although finger millet is considered the future food, there are currently very few reports on how it reacts to salinity stress. This study aimed to compare the responses of twenty-seven native finger millet landraces under salt stress (0 mM to 250 mM NaCl in Hoagland solution). The degree of germination, seedling growth, biomass output, carotenoid content, membrane damage, and mineral accumulation were used to assess stress tolerance. With an increase in NaCl concentration, all landraces saw a drop in biomass production, shoot length, root length, and germination percentage. The decline was greater in ST-JA-SM and less in ST-JA-WA among landraces. All of the landraces showed a decrease in carotenoid content (108.76 ± 7.56 to 1.96 ± 0.83 µg mg⁻¹ FW) with increasing salt concentration, whereas malondialdehyde (MDA) content (0.17 ± 0.05 to 8.36 ± 0.53 µM g⁻¹ FW) was found to be increasing due to the stress imposed by salinity. The salt stress-sensitive landraces ST-JA-SM had greater MDA than the salt stress-tolerant ST-JA-WA. NaCl stress increased sodium and potassium levels in the seedlings of all the landraces. The magnitude of Na⁺ and K⁺ ions accumulation was higher in ST-JA-WA and less in ST-JA-SM. The salt tolerance indices for K⁺ ranged from 0.94 to 1.19, and that of Na⁺ ranged from 0.71 to 1.51. Combined these findings suggest that ST-JA-SM is stress-sensitive and ST-JA-WA is a stress-tolerant landrace that can be exploited in crop improvement programs. Additionally, it can be used as a participant in research on stress tolerance alleviation with noble elements like silicon or selenium.</p>","PeriodicalId":9841,"journal":{"name":"Cereal Research Communications","volume":"283 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139645308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Appraisal of genetic variability and detection of sequence polymorphism in the Rc and Rd loci among the pigmented and non-pigmented genotypes of rice","authors":"Durgadatta Meher, Arpita Das, Joydeep Banerjee, Sudip Bhattacharya, Torit Baran Bagchi, Krishnendu Pramanik","doi":"10.1007/s42976-023-00482-3","DOIUrl":"https://doi.org/10.1007/s42976-023-00482-3","url":null,"abstract":"<p>Diverse, traditional landraces of <i>indica</i> rice with varying pigmentations are grown at the foothills of Eastern Himalayas. Interaction between two rice genes <i>Rc</i> and <i>Rd</i> is the major determining factor for diverse pigmentations. <i>Rd</i> regulates the synthesis of anthocyanidin, whereas <i>Rc</i> encodes a transcription factor for the expression of proanthocyanidins in rice. This study involved 24 genotypes of rice with varying pigmentation, collected from the Himalayan region and Eastern India for appraisal of genetic variability, character association, diversity regarding yield and grain-quality traits, and detection of sequence variation concerning <i>Rc</i> and <i>Rd</i> loci. Results revealed substantial genetic variability with significant genotype × year interaction for most of the traits. The tested genotypes were grouped into nine clusters based on Euclidean distance. Analyses of coding DNA and amino acid sequences revealed non-functional <i>Rc</i> gene with 14-bp deletion without or with a single nucleotide addition in the sixth exon, which led to premature stop codon formation or frameshift mutation in most of the black and white rice on the contrary to red rice genotypes. Furthermore, only a single white rice genotype was detected with a premature stop codon formation in the analyzed region of <i>Rd</i>. That mutation might be the probable cause for obstruction in anthocyanin and proanthocyanidin biosynthesis. Further studies are needed to identify the point mutations and premature stop codon (if any) in the <i>Rd</i> locus of various <i>indica</i> rice genotypes from these regions to unveil the evolutionary relevance of the <i>Rd</i> allele for aberrated dihydroflavonol-4-reductase protein formation.</p>","PeriodicalId":9841,"journal":{"name":"Cereal Research Communications","volume":"6 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139583465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Understanding the biochemical, physiological, molecular, and synthetic biology approaches towards the development of C4 rice (Oryza sativa L.)","authors":"Jyoti Prakash Sahoo, Debashis Mahapatra, Manaswini Mahapatra, Tuward J. Dweh, Salma Kayastha, Pranaya Pradhan, Swapan Kumar Tripathy, Kailash Chandra Samal, Abinash Mishra, Manasi Dash, Spandan Nanda","doi":"10.1007/s42976-024-00489-4","DOIUrl":"https://doi.org/10.1007/s42976-024-00489-4","url":null,"abstract":"<p>Crop yields must drastically increase to meet the demands of a rapidly increasing global population. One current option being investigated is the augmentation of photosynthetic capacity by incorporating the C₄ photosynthetic pathway into C₃ crops such as rice to significantly increase production. Crops with improved photosynthetic mechanisms would make better use of the solar radiation that can be converted into yield. This will help to boost grain yield, reduce water loss, and increase nitrogen use efficiency, particularly in hot and dry conditions. Identifying and manipulating the genes required to install C₄ photosynthesis in rice is a big challenge. However, the international C₄ rice collaboration, is formed to test the possibility of incorporating the C₄ mechanism into rice. This article summarizes the parameters that must be changed in rice in order for the C₄ pathway to be successfully introduced. It also examines the biochemistry, physiology, molecular biology, and synthetic biology approaches between the C₃ and C₄ photosynthetic processes.</p>","PeriodicalId":9841,"journal":{"name":"Cereal Research Communications","volume":"13 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139556605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Arsenic stress responses in sensitive and tolerant rice of North-East, India","authors":"Thorny Chanu Thounaojam, Thounaojam Thomas Meetei, Yumnam Bijilaxmi Devi, Bhaben Tanti, Sanjib Kumar Panda, Hrishikesh Upadhyaya","doi":"10.1007/s42976-023-00488-x","DOIUrl":"https://doi.org/10.1007/s42976-023-00488-x","url":null,"abstract":"<p>Arsenic (As) significantly affects production and quality of rice (<i>Oryza sativa L</i>), the global staple food. Therefore, there is a need for As tolerant rice and so, present work is designed to identify the highest As tolerant and sensitive rice cultivars of North-East (NE), India, and the key mechanisms for the sensitivity and tolerance. Hydroponically grown twenty rice cultivars of NE were analyzed for the changes in growth, As content and Arsenic tolerant index % (ATI%) after 7th day of As treatments (0, 100 and 300 µM), where Phougak rice was found to be the highest tolerant having highest ATI% while cultivar with IC no. 200507 with least ATI%, the highly sensitive to As. The sensitivity and tolerance of the rice against As were further analyzed through the evaluation of morphological and histochemical changes, relative water content, chlorophyll content, hydrogen peroxide (H₂O₂) and lipid peroxidation level, and antioxidant metabolites and enzymes activities. Arsenic significantly increased H₂O₂ level, resulted into lipid peroxidation and cell death in sensitive rice, moreover, scanning electron microscopy also revealed damaging effect of As in the plant. However, no damaging effect was observed in the tolerant rice, but considerable increase of ascorbate and glutathione content and activity of antioxidant enzymes were recorded. Hence, high accumulation of As induced ROS in sensitive rice, while constant detoxification of the ROS in tolerant rice by efficient antioxidant defence system could be the reasons for the sensitivity of 200507 and tolerance of Phougak rice to As stress.</p>","PeriodicalId":9841,"journal":{"name":"Cereal Research Communications","volume":"106 1S 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139556602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Drought-induced variations in nutritional composition of grain and bran of contrasting rice genotypes","authors":"","doi":"10.1007/s42976-023-00485-0","DOIUrl":"https://doi.org/10.1007/s42976-023-00485-0","url":null,"abstract":"<h3>Abstract</h3> <p>Drought is a key environmental element that affects rice (<em>Oryza sativa</em> L.) productivity and quality. Control and drought-treated rice grain (milled) and bran fractions of two contrasting rice genotypes (N22-drought-tolerant and IR64-drought-sensitive) were evaluated based on proximate composition, vitamins, minerals, and nutritional quality indices. Except for carbohydrates and amylose content, bran from both genotypes had higher concentrations of all parameters (proximate, minerals, vitamins, phenols, amino acids, and phytic acid) analyzed in this study. Protein level ranged from 9–11% in grain to 13–15% in bran. Macronutrients (Na, Ca, K, Mg, P) increased after drought in both genotypes. Under drought, thiamine level ranged from 0.07 to 1.97 mg/100 g, riboflavin level from 0.02 to 0.43 mg/100 g, and <em>α</em>-tocopherol from 1.67 to 8.34 mg/100 g in both fractions. N22 showed higher amylose (20.44% and 4.84%) and phenol (0.46 mg/GAE and 2.54 mg/GAE) content in both grain and bran fractions under drought condition. Proline accumulated at higher concentration (2.732 g/100 g) than other amino acids in N22 under drought. Phytic acid ranged from 0.213 to 2.752 g/100 g in drought. To the best of our knowledge, this is the first preliminary report on nutritional quality analysis of rice grain and bran under drought stress. The current findings may help breeders in development of high-quality, drought-tolerant rice varieties, and will also provide a strong foundation for utilizing bran as an essential food ingredient to improve human nutrition and may help breeders to use this tolerant genotype as better parents in further crossings.</p>","PeriodicalId":9841,"journal":{"name":"Cereal Research Communications","volume":"56 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139515939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of Tos17 LTR retrotransposon movements in rice (Oryza sativa L.) under nickel and boron stress","authors":"Sinan Meriç, Alp Ayan, Burcu Gündüz, Can Özpirinçci, Özge Çelik, Çimen Atak","doi":"10.1007/s42976-023-00486-z","DOIUrl":"https://doi.org/10.1007/s42976-023-00486-z","url":null,"abstract":"<p>Heavy metal and metalloid pollution caused by the industrialization became a leading stress factors for agricultural plants. The increase in the amount of nickel and boron in agricultural areas due to mining and increasing industrial activity is an important agricultural constraint. The difference between deficiency and toxicity levels of these heavy metal and metalloid is extremely critical. Nickel and boron are important micronutrients for plant growth, while they become toxic at critical densities. Plants exhibit different responses to these pollutants. It is essential to find specific biomarkers to discriminate the tolerant varieties to develop elite varieties. Transposable elements are known to have an efficient role against environmental stress factors. In this research, we evaluated the potential use of Tos17 retrotransposon movement as a molecular marker to identify the stress tolerances of two <i>Oryza sativa</i> L. varieties against nickel and boron pollutants.</p>","PeriodicalId":9841,"journal":{"name":"Cereal Research Communications","volume":"15 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139500420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Obtaining dihaploid lines of winter bread wheat with complex resistance to rust and common bunt by anther culture in vitro","authors":"I. Zambriborshch, O. Shestopal, V. Traskovetskaya, O. Vasiliev, O. Halaiev, M. Halaieva, O. Afinogenov, M. Chekalova","doi":"10.1007/s42976-023-00466-3","DOIUrl":"https://doi.org/10.1007/s42976-023-00466-3","url":null,"abstract":"<p>We investigated the anther culture (AC) efficiency of nine genotypes of winter bread wheat (<i>Triticum aestivum</i> L.). The genotype dependency was assessed during the induction of the androgenic callus, i.e., embryo-like structures (ELSs), green-, albino- and acclimatizated plantlets. The highest level of callus formation were shown for samples 120/20 (114.39 ELS per 100 anthers—ELS/100A) and 132/20 (16.26 ELS/100A). The number of green plantlets per 100 anthers (GP/100A) varied from 0 to 3.05 GP/100A with a mean of 0.71 GP/100A. The acclimatized plantlets (ADPs) per 100 regenerated green plantlets ranged in each combination, from 32.00 to 62.50 ADP/100GP with an average value of 35.92. Between 12.50 and 60.00 doubled haploid (DH) plants per 100 acclimatized plantlets (DH/100ADP), depending on the combination, with a mean of 29.41% were recovered. Fertile plants in the anthers culture 5 out of 9 studied genotypes were obtained. Seventeen dihaploid lines with complex rust resistance and common bunt (8–9 points) were obtained. Two clusters of complex resistance genes were identified in the ten studied DH lines: Lr26/Sr31/Pm8/Yr9 and Lr34/Yr18/Sr58/Pm38.</p>","PeriodicalId":9841,"journal":{"name":"Cereal Research Communications","volume":"36 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139501731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}