Obtaining dihaploid lines of winter bread wheat with complex resistance to rust and common bunt by anther culture in vitro

IF 1.6 4区农林科学 Q2 AGRONOMY

Cereal Research Communications Pub Date : 2024-01-18 DOI:10.1007/s42976-023-00466-3

I. Zambriborshch, O. Shestopal, V. Traskovetskaya, O. Vasiliev, O. Halaiev, M. Halaieva, O. Afinogenov, M. Chekalova

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Abstract

We investigated the anther culture (AC) efficiency of nine genotypes of winter bread wheat (Triticum aestivum L.). The genotype dependency was assessed during the induction of the androgenic callus, i.e., embryo-like structures (ELSs), green-, albino- and acclimatizated plantlets. The highest level of callus formation were shown for samples 120/20 (114.39 ELS per 100 anthers—ELS/100A) and 132/20 (16.26 ELS/100A). The number of green plantlets per 100 anthers (GP/100A) varied from 0 to 3.05 GP/100A with a mean of 0.71 GP/100A. The acclimatized plantlets (ADPs) per 100 regenerated green plantlets ranged in each combination, from 32.00 to 62.50 ADP/100GP with an average value of 35.92. Between 12.50 and 60.00 doubled haploid (DH) plants per 100 acclimatized plantlets (DH/100ADP), depending on the combination, with a mean of 29.41% were recovered. Fertile plants in the anthers culture 5 out of 9 studied genotypes were obtained. Seventeen dihaploid lines with complex rust resistance and common bunt (8–9 points) were obtained. Two clusters of complex resistance genes were identified in the ten studied DH lines: Lr26/Sr31/Pm8/Yr9 and Lr34/Yr18/Sr58/Pm38.

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通过花药离体培养获得具有锈病和普通穗疫病复合抗性的冬季面包小麦二倍体品系

我们研究了九种冬季面包小麦（Triticum aestivum L.）基因型的花药培养（AC）效率。在诱导雄性胼胝体（即胚状结构（ELS）、绿色、白化和适应性小苗）的过程中，对基因型依赖性进行了评估。胼胝体形成水平最高的是 120/20 号样品（每 100 个花药有 114.39 个胚状结构-ELS/100A）和 132/20 号样品（每 100 个花药有 16.26 个胚状结构-ELS/100A）。每 100 个花药中绿色小植株的数量（GP/100A）从 0 到 3.05 GP/100A 不等，平均为 0.71 GP/100A。在每个组合中，每 100 个再生绿色小植株的适应性小植株（ADP）在 32.00 至 62.50 ADP/100GP 之间变化，平均值为 35.92。根据组合的不同，每 100 个适应性小植株中可获得 12.50 到 60.00 个加倍单倍体（DH）植株（DH/100ADP），平均值为 29.41%。在研究的 9 个基因型中，有 5 个在花药培养中获得了可育植株。获得了 17 个具有复合抗锈病和普通钝点（8-9 点）的二倍体品系。在研究的 10 个 DH 株系中发现了两个复合抗性基因簇：Lr26/Sr31/Pm8/Yr9 和 Lr34/Yr18/Sr58/Pm38。

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来源期刊

Cereal Research Communications AGRONOMY-

CiteScore

3.40

自引率

6.20%

发文量

审稿时长

6-12 weeks

期刊介绍： This journal publishes original papers presenting new scientific results on breeding, genetics, physiology, pathology and production of primarily wheat, rye, barley, oats and maize.