China Journal of Chinese Matera Medica最新文献_第2页

Inhibitory effect of resveratol on ischemia reperfusion-induced cardiocyte apoptosis and its relationship with PI3K-Akt signaling pathway 白藜芦醇对缺血再灌注诱导的心肌细胞凋亡的抑制作用及其与PI3K-Akt信号通路的关系

China Journal of Chinese Matera Medica Pub Date : 2012-08-01 DOI: 10.4268/CJCMM20121529

Dong-wei He, Xin-wei Liu, Y. Pang, Liu Liu

{"title":"Inhibitory effect of resveratol on ischemia reperfusion-induced cardiocyte apoptosis and its relationship with PI3K-Akt signaling pathway","authors":"Dong-wei He, Xin-wei Liu, Y. Pang, Liu Liu","doi":"10.4268/CJCMM20121529","DOIUrl":"https://doi.org/10.4268/CJCMM20121529","url":null,"abstract":"OBJECTIVE To study the effect of resveratol on ischemia reperfusion-induced cardiocyte apoptosis and its relationship with PI3K-Akt signaling pathway. METHOD Fifty male SD rats were divided randomly into five groups: the control group (SH group), the ischemia reperfusion group (I/R group), the resveratol pretreatment group (Res group), the resveratol pretreatment + wortmannin group (Res +Wom group) and the ischemia reperfusion + wortmannin group (I/R + Wom group). The myocardial ischemia model was established by ligating left coronary artery for 45 min followed by 120 min reperfution, in order to observe the contents of NOS and NO. Cardiac myocyte apoptosis was determined by terminal deoxynueleotidyl transferase-mediated dUTP nick end labeling (TUNEL). Bcl-2 and Bax proteins were detected by immunohistochemistry. The t-Akt and p-Akt signaling protein expressions were determined by Western blotting analysis. RESULT Compared with the I/R groups and the Res + Wom group, the Res group showed significant increase in the expressions of NOS, NO, Bcl-2 protein and p-Akt and notable decrease in cardiocyte apoptosis and Bax/Bcl-2. The difference of above indicators showed a statistical significance (P<0.05). Furthermore, above changes can be blocked by wortmannin, a specific blocker of PI3K-Akt signaling pathway, indicating a statistical significance in their changes (P<0.05). CONCLUSION Resveratol can inhibit the ischemia reperfusion-induced cardiocyte apoptosis, in which PI3K-Akt signaling pathway gets involved.","PeriodicalId":9835,"journal":{"name":"China Journal of Chinese Matera Medica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79171096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

[Clinical study on prevention efficacy of Jianpi Huatan Fang in treating non-alcoholic fatty liver disease in children]. 健脾化痰方预防儿童非酒精性脂肪肝疗效的临床研究

China Journal of Chinese Matera Medica Pub Date : 2012-08-01 DOI: 10.4268/CJCMM20121625

W. Fang

{"title":"[Clinical study on prevention efficacy of Jianpi Huatan Fang in treating non-alcoholic fatty liver disease in children].","authors":"W. Fang","doi":"10.4268/CJCMM20121625","DOIUrl":"https://doi.org/10.4268/CJCMM20121625","url":null,"abstract":"OBJECTIVE To verify the clinical efficacy of Jianpi Huatan Fang in treating non-alcoholic fatty liver disease in children. METHOD Sixty children patients with non-alcoholic liver disease were randomly assigned to 2 groups of 30 patients each , being treated with Jianpi Huatan Fang and the Vitamin E Recipe with a span of 3 months, respectively. The changes of the FINS, FPG, AST, ALT, TG, CHO, HDL, LDL, HOMA-IR, IAI after and before the treatments in both groups as well as the ultrasound imaging changes of liver were observed. RESULT The Chinese medicine group saw higher total effective rate (83.3%) than the Vitamin E group (46.7%) with statistic difference. In addition, after the treatments, both groups saw the significant decrease of the levels of serum lipid metabolic indexes and insulin resistance indexes. Both treatments could effectively enhance the function of liver, but Chinese medicine showed better efficacy. The Chinese medicine group also saw the decrease of ATC, TG, LDL and increase of HDL-C (P < 0.05 (see character in text) P <0.01) after the treatment, showing that the Chinese medicine could effectively enhance blood fat; while the Vit E group did not showed improvement in TC, LDL-C, HDL-C. CONCLUSION Jianpi Huatan Fang showed good efficacy in treating non-alcoholic fatty liver in children with no observable toxic side effects, thus clinic use shall be recommended.","PeriodicalId":9835,"journal":{"name":"China Journal of Chinese Matera Medica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81991955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Study on reference extracts for quality assessment on traditional Chinese medicines based on akebiae saponin 以苦参皂苷为基础的中药质量评价参比提取物的研究

China Journal of Chinese Matera Medica Pub Date : 2012-08-01 DOI: 10.4268/CJCMM20121614

Yu Xianda

引用次数: 1

Effects of different light quality on growth,active ingredients and enzymes activities of Salvia miltiorrhiza 不同光质对丹参生长、活性成分及酶活性的影响

China Journal of Chinese Matera Medica Pub Date : 2012-07-01 DOI: 10.4268/CJCMM20121405

Zongsuo Liang, Qian Li, Wen-Hui Xu

{"title":"Effects of different light quality on growth,active ingredients and enzymes activities of Salvia miltiorrhiza","authors":"Zongsuo Liang, Qian Li, Wen-Hui Xu","doi":"10.4268/CJCMM20121405","DOIUrl":"https://doi.org/10.4268/CJCMM20121405","url":null,"abstract":"OBJECTIVE This study aimed to investigate the effects of different light quality on the growth, accumulation of active ingredients and enzymes activities of Salvia miltiorrhiza. METHOD The seedlings of S. miltiorrhiza were treated by different light quality, and relative parameters were measured. The data was statistically processed. RESULT Plant height was significantly decreased with supplemental blue light (WB), and the root length, root diameter, root fresh weight and root dry weight were significantly increased with supplemental red light (WR). Salvianolic acid B concentration in S. miltiorrhiza was highly increased by supplemental blue and red light, but tanshinone IIA concentration was not significantly affected by supplemental blue and red light. Enzymes activities of SOD, POD, PAL, TAT and PPO in S. miltiorrhiza were significant increased by supplemental blue light, and enzymes activities of POD, TAT and PPO were significant increased by supplemental red light. CONCLUSION The root growth of S. miltiorrhiza was greatly promoted by supplemental red light (WR). Salvianolic acid B concentration in S. miltiorrhiza was highly increased by supplemental blue and red light. Enzymes activities of TAT and PPO in S. miltiorrhiza were significant increased by supplemental blue light and red light.","PeriodicalId":9835,"journal":{"name":"China Journal of Chinese Matera Medica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91215499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Effect of icariin on learning and memory abilities and activity of cholinergic system of senescence-accelerated mice SAMP10]. [淫羊藿苷对衰老加速小鼠SAMP10学习记忆能力及胆碱能系统活性的影响]。

China Journal of Chinese Matera Medica Pub Date : 2012-07-01 DOI: 10.4268/CJCMM20121418

Gao Lin-na

{"title":"[Effect of icariin on learning and memory abilities and activity of cholinergic system of senescence-accelerated mice SAMP10].","authors":"Gao Lin-na","doi":"10.4268/CJCMM20121418","DOIUrl":"https://doi.org/10.4268/CJCMM20121418","url":null,"abstract":"OBJECTIVE To investigate the effect of icariin (ICA) on learning and memory abilities and cholinergic system in senescence-accelerated mice SAMP10. METHOD The 8-month-old senescence-accelerated mice were randomly divided into the model SAMP10 group and the positive Donepezil group (1 mg x kg(-1)) and ICA groups (50, 100, 200 mg x kg(-1)), with 12 mice in each group. Another 12 8-month-old mice SAMR1 were selected as the normal control group. After 30 days of oral administration, Morris water maze, SMG-2 water maze and experimental platform were used to test the effects of ICA on learning and memory abilities of SAMP10 groups. By colorimetric determination of AChE activity in the cortex, enzyme-linked immunosorbent assay detection of ACh, ChAT, MCBC of the cerebral cortex, the effect of ICA on the cholinergic system of SAMP10 was observed. RESULT ICA could improve the abilities of space exploration and positioning navigation of SAMP10, shorten the latency in SMG-2 water maze, enhance their jumping ability in response to the passive test, and increase levels of ACh, ChAT, MCBC in the cerebral cortex of SAMP10. But its active effect on AChE in SAMP10 cortex was not obvious. CONCLUSION Different doses of icariin can improve learning and memory abilities of SAMP10 to varying degrees, which may be related to its effect on the cholinergic system.","PeriodicalId":9835,"journal":{"name":"China Journal of Chinese Matera Medica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80140985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Experimental study on anaphylactoid reactions induced by different components of Shengmai injection on Beagle dogs 生脉注射液不同成分致Beagle犬类过敏反应的实验研究

China Journal of Chinese Matera Medica Pub Date : 2012-07-01 DOI: 10.4268/CJCMM20121304

H. Ping

引用次数: 1

[Distribution of puerarin contained in Zige freeze-dried powder injection in rat liver and kidney]. [紫葛粉冻干粉注射液葛根素在大鼠肝、肾中的分布]。

China Journal of Chinese Matera Medica Pub Date : 2012-06-01 DOI: 10.4268/CJCMM20121137

Wang Qin, X. Mao, Deng Lijuan, Wang Yongsheng, Mukaram Kipaytul, Xu Xiaoyu

{"title":"[Distribution of puerarin contained in Zige freeze-dried powder injection in rat liver and kidney].","authors":"Wang Qin, X. Mao, Deng Lijuan, Wang Yongsheng, Mukaram Kipaytul, Xu Xiaoyu","doi":"10.4268/CJCMM20121137","DOIUrl":"https://doi.org/10.4268/CJCMM20121137","url":null,"abstract":"OBJECTIVE To investigate the distribution process of puerarin contained in Zige freeze-dried powder injection in rat liver and kidney and the safety of Zige freeze-dried powder injection. METHOD Rats were divided into the Zige freeze-dried powder injection group and the puerarin freeze-dried powder injection control group randomly. The liver and kidney samples were collected at 5, 10, 20, 30, 45, 60 and 120 min after intravenous administration of puerarin (26.7 microg x g(-1)) through caudal vein and detected by HPLC. RESULT The concentration of puerarin in kidney reached the max value of 58.12 microg x g(-1) for the Zige freeze-dried powder injection group and 71.28 microg x g(-1) for the Puerarin freeze-dried powder injection group. The value of AUC(0-2h) was 26.24 microg x h x g(-1) for the Zige freeze-dried powder injection group and 35.24 microg x h x g(-1) for the puerarin freeze-dried powder injection group, MRT(0-2h) was 0. 39 h for the Zige freeze-dried powder injection group and 0. 42 h for the puerarin freeze-dried powder injection control group. Compared with the control group, the Zige freeze-dried powder injection group showed a significant decrease in Cmax and AUC(0-2h) (P < 0.05), with no notable difference in peak time tmax and MRT(0-2h). The two groups showed no obvious difference in tmax Cmax, AUC(0-2h) and MRT(0-2h) of puerarin in rat kidney. CONCLUSION Compared with Zige freeze-dried powder injection, Zige freeze-dried powder injection can reduce the distribution of puerarin in rat kidney, with no obvious change in the elimination of puerarin in rat kidney. It also showed no significant change in distribution and elimination in liver. This indicates that Zige freeze-dried powder injection is safer to kidney than puerarin freeze-dried powder injection.","PeriodicalId":9835,"journal":{"name":"China Journal of Chinese Matera Medica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88383044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Determination of α-pinene and octyl acetate contents in Boswellia serrata 锯齿乳香中α-蒎烯和乙酸辛酯含量的测定

China Journal of Chinese Matera Medica Pub Date : 2012-05-01 DOI: 10.4268/CJCMM20121018

Z. Song, L. Xia, Z. Wei, Y. Cao, Li Zhang, Z. Liu

{"title":"Determination of α-pinene and octyl acetate contents in Boswellia serrata","authors":"Z. Song, L. Xia, Z. Wei, Y. Cao, Li Zhang, Z. Liu","doi":"10.4268/CJCMM20121018","DOIUrl":"https://doi.org/10.4268/CJCMM20121018","url":null,"abstract":"OBJECTIVE: To establish method for determining the contents of alpha-pinene and octyl acetate in Boswellia serrata, in order to provide preference for making quality standards for B. serrata and processed B. serrata. METHOD: Application of orthogonal design was employed to optimize the solvent, solvent quantity and extraction time. The GC-MS analysis was performed on a Rxi-5ms silica capillary column, running in the electron impact (EI) mode, with ion trap and injector temperature of 200 degrees C and 250 degrees C, respectively. The column oven was initially 50 degrees C and was held for 1 min after injection, followed by temperature ramping at 5 degrees C x min(-1) up to 130 degrees C, holding for 1 min. 1 microL of samples solution were injected in the split mode (1:60). Helium was the carrier gas. The mass spectrometer was set to scan m/z 45450 with an ionizing voltage at 70 eV. RESULT: Sample solutions were prepared for 50-fold dose by ultrasonic extraction with hexane for 30 min. The content of alpha-pinene and octyl acetate in 10 batches of B. serrata were 0.021 3-0.149 5, 2.519 6-9.098 0 mg x g(-1), respectively. And, those of alpha-pinene and octyl acetate in processed B. serrata were 0.015 9-0.065 9, 0.801 0-12.812 2 mg x g(-1). CONCLUSION: The method is a stable and reliable for determining the contents of alpha-pinene and octyl acetate in B. serrata.","PeriodicalId":9835,"journal":{"name":"China Journal of Chinese Matera Medica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86822010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Grey relational analysis on fingerprint characteristics of different eluted parts of Euphorbia humifusa and antifungal effect 葎草不同洗脱部位指纹特征与抑菌效果的灰色关联分析

China Journal of Chinese Matera Medica Pub Date : 2012-03-01 DOI: 10.4268/CJCMM20120508

L. Zhijian

引用次数: 2

Effect of Euphorbia kansui on urination and kidney AQP2, IL-1β and TNF-α mRNA expression of mice injected with normal saline 大麻黄对生理盐水注射小鼠排尿及肾脏AQP2、IL-1β、TNF-α mRNA表达的影响

China Journal of Chinese Matera Medica Pub Date : 2012-03-01 DOI: 10.4268/CJCMM20120514

Huiyu Li, F. Lei, Yugang Wang, Xin-yue Xiao, Jun Hu, Xianlong Cheng, Dongming Xing, Lei Hua, Ruichao Lin, L. Du

引用次数: 2