China Journal of Chinese Matera Medica最新文献

Studies on sexual organs and embryological development morphology of Pterocypsela formosana 台湾翼柏性器官及胚胎发育形态之研究

China Journal of Chinese Matera Medica Pub Date : 2015-01-01 DOI: 10.4268/CJCMM20150207

黄衡宇, 龙华, 马雪妮

引用次数: 0

LC-MS/MS测定6种丹参主要有效成分及其脑靶向分布研究 LC-MS/MS测定6种丹参主要有效成分及其脑靶向分布研究

China Journal of Chinese Matera Medica Pub Date : 2014-01-01 DOI: 10.4268/CJCMM20140931

刘胜敏, 孙晓波, 杨志宏

引用次数: 1

Effect of Angelica sinensis polysaccharide on expression of telomere,telomerase and P53 in mice aging hematopoietic stem cells 当归多糖对小鼠衰老造血干细胞端粒、端粒酶及P53表达的影响

China Journal of Chinese Matera Medica Pub Date : 2013-01-01 DOI: 10.4268/CJCMM20131428

王璐, 王建伟, 张先平, 李静, 刘俊, 魏强, 王亚平, 徐春燕

引用次数: 3

Determination of endogenous amino Acids in brain tissues after cerebral ischemia by RRLC-QQQ RRLC-QQQ法测定脑缺血后脑组织内源氨基酸

China Journal of Chinese Matera Medica Pub Date : 2013-01-01 DOI: 10.4268/CJCMM20130526

杨洪军, 梁日欣, 陈畅, 朱丽, 吴宏伟, 李韶菁, 高健, 杨更亮, 吴传鸿

引用次数: 0

Angelica sinensis polysaccharides delay aging of hematopoietic stem cells through inhibitting oxidative damge 当归多糖通过抑制氧化损伤延缓造血干细胞衰老

China Journal of Chinese Matera Medica Pub Date : 2013-01-01 DOI: 10.4268/CJCMM20130323

王建伟, 张先平, 姜蓉, 魏强, 王乾兴, 王亚平, 徐春燕, 陈斌

引用次数: 2

Effect of Qingxin Kaiqiao formula and saponin on learning and memory abilities and expression of apoptosis signal transducers Aβ and βAPP in AD rat brain 清心开翘方和皂苷对AD大鼠脑学习记忆能力及凋亡信号转导因子Aβ和βAPP表达的影响

China Journal of Chinese Matera Medica Pub Date : 2012-10-01 DOI: 10.4268/CJCMM20121923

Sheng-hui Chu, Hai-Yan Hu, Zhan-wang Tan, Xiang Chen, Wen-hua Wang, Xiao-yan Zhang

{"title":"Effect of Qingxin Kaiqiao formula and saponin on learning and memory abilities and expression of apoptosis signal transducers Aβ and βAPP in AD rat brain","authors":"Sheng-hui Chu, Hai-Yan Hu, Zhan-wang Tan, Xiang Chen, Wen-hua Wang, Xiao-yan Zhang","doi":"10.4268/CJCMM20121923","DOIUrl":"https://doi.org/10.4268/CJCMM20121923","url":null,"abstract":"OBJECTIVE To study the effect of qingxin kaiqiao formula and saponin on the learning and memory ability and the expression of the apoptosis signal transducers Abeta and betaAPP in AD rat brain. METHOD The comparative observation method was adopted for the animal test. Forty male SD rats were randomly divided into five groups, namely the normal group, the model group, the aricept group, the qingxin kaiqiao formula group and the saponin group, with eight rats in each group. Abeta(25-35) (10 g x L(-1)) was injected into their bilateral amygdala to establish the AD rat model. Since the next day, they were intragastrically administered with Aricept (1.67 mg x kg(-1)), Qingxin Kaiqiao decoction (12.67 mL x kg(-1)), saponin (6.30 mg x kg(-1)) and double distilled water filling for 2 weeks to observe their spatial memory ability in a Morris water maze and study the expression of Caspase-3, Abeta and betaAPP in brain tissues by immunohistochemistry. RESULT Each traditional Chinese medicine groups showed significant improvement in the learning and memory ability of AD rats and notable differences (P < 0.05, P < 0.01) compared with the control group. The qingxin kaiqiao formula group and the saponin group showed a decrease in the expressions of Caspase-3, Abeta and betaAPP in cerebral cortex and hippocampus area, displaying notable differences (P < 0.01, P < 0.05) compared with the control group. CONCLUSION qingxin kaiqiao formula and saponin can obviously improve the learning and memory ability of AD rats with by decreasing the expression of Caspase-3, Abeta and betaAPP in cortex and hippocampus.","PeriodicalId":9835,"journal":{"name":"China Journal of Chinese Matera Medica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80468930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

[Drug releasing characteristics evaluation to multi-drug delivery system of tongmai pellets]. 【通脉微丸多药给药系统的释药特性评价】。

China Journal of Chinese Matera Medica Pub Date : 2012-10-01 DOI: 10.4268/CJCMM20121911

Yuan Dan-hong

引用次数: 0

[Metabolomic study of flower buds of Tussilago farfara in different development stages by GC-MS]. [用气相色谱-质谱法研究柞蚕不同发育阶段花蕾的代谢组学]。

China Journal of Chinese Matera Medica Pub Date : 2012-10-01 DOI: 10.4268/CJCMM20121906

Xue Shui-yu

{"title":"[Metabolomic study of flower buds of Tussilago farfara in different development stages by GC-MS].","authors":"Xue Shui-yu","doi":"10.4268/CJCMM20121906","DOIUrl":"https://doi.org/10.4268/CJCMM20121906","url":null,"abstract":"OBJECTIVE: Plant metabolomics combined with GC-MS was used to investigate metabolic fingerprinting of Tussilago farfara at different growth stages. METHOD: Dried Samples were extracted by two-phase solvent system to obtain polar and nonpolar parts, which were subjected to GC-MS analysis. Metabolites were identified by NIST data base search and comparison with the authentic standards. The data were introduced into SIMCA-P 11.0 software package for multivariate analysis after pretreatment. RESULT: Fifty-four metabolites were identified, including 35 polar metabolites and 19 nonpolar compounds. The score plot for PCA showed clear separation of the different development stages of flower buds of T. farfara, showing a trend of gradual change. Samples of October, November, December were in close proximity on the plot, indicating that the metabolome of these three periods was similar, samples from September (early development) and March (after flowering) were far away, showing big chemical differences. Content comparison results of some representative metabolites reveals that, the content of proline, lysine and linoleic acid increased gradually to the highest in the medium term, but sharply decreased to the lowest after flowering; the content of malic acid and citric acid were the lowest in the medium term; sucrose content decreased gradually, and then reached the lowest level after blooming. CONCLUSION: It is obvious that metabolites of the early development and flowering stage were quite different with those of the traditional harvest time, suggesting that they can not be used as traditional medicine. This study will provide a research basis for harvest time determination and bioactive compounds of T. farfara.","PeriodicalId":9835,"journal":{"name":"China Journal of Chinese Matera Medica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77168464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Design technology of traditional Chinese medicine component and formulation 中药成分及配方设计技术

China Journal of Chinese Matera Medica Pub Date : 2012-10-01 DOI: 10.4268/CJCMM20121933

Yuan Dan-hong

引用次数: 1

Effect of sophoridine and TLR4/MD-2 blocking agent on pathway of LPS-induced RAW264.7 macrophage TLR4-NF-κB-TNF-α 槐定与TLR4/MD-2阻断剂对lps诱导RAW264.7巨噬细胞TLR4- nf -κB-TNF-α通路的影响

China Journal of Chinese Matera Medica Pub Date : 2012-10-01 DOI: 10.4268/CJCMM20122022

Yan-Rong Wang, Feng Yang, Jing Liu, Ya Zhou

{"title":"Effect of sophoridine and TLR4/MD-2 blocking agent on pathway of LPS-induced RAW264.7 macrophage TLR4-NF-κB-TNF-α","authors":"Yan-Rong Wang, Feng Yang, Jing Liu, Ya Zhou","doi":"10.4268/CJCMM20122022","DOIUrl":"https://doi.org/10.4268/CJCMM20122022","url":null,"abstract":"OBJECTIVE: To study the effect of sophoridine and TLR4/MD-2 blocking agent on pathway of LPS-induced RAW264. 7 macrophage TLR4-NF-kappaB-TNF-alpha in and its pharmacological mechanism of antiendotoxin. METHOD: RAW264. 7 macrophages were cultured and divided into 5 groups, namely the blank control group, the LPS model group, the sophoridine + LPS group, the TLR4/MD-2 blocking agent + LPS group and the anti-TLR4/MD-2 + sophoridine + LPS group. Cells and cell culture fluids were collected at 120 min after the each group was processed. The expression of TLR4 protein was measured by western blot, the distribution and expression of NF-kappaB protein were measured by immunocytochemistry, and the expression of NF-kappaB and TNF-alpha mRNA were measured by western blot and reverse transcriptase polymerase chain reaction (RT-PCR). The content of TNF-alpha in the cell supernatant was detected by using radioimmunoassay. RESULT: Compared with the LPS group, the expression of TLR4 protein, NF-kappaB mRNA, the rate of NF-kappaB entry the nucleus, TNF-alpha mRNA and TNF-alpha content in the cell supernatant were significantly decreased in the sophoridine + LPS group (P < 0.01). The rate of NF-kappaB entry the nucleus and TNF-alpha in the TLR4/MD-2 blocking agent + LPS group and the TLR4/MD-2 blocking agent + sophoridine + LPS group were notablly lower than that of the LPS model group (P < 0.01), close to that of the blank control group. However, there was no statistical significance between the two groups. CONCLUSION: TLR4/MD-2 may be one of sophoridine's targets. Sophordine's inhibitory effect on pathway activity of TLR4-NF-kappaB-TNF-alpha may be one of its antiendotoxin mechanisms.","PeriodicalId":9835,"journal":{"name":"China Journal of Chinese Matera Medica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75803098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1