Cell Biology International最新文献

Cancer Classification Through p53 Hotspot Mutations: An Ensemble Learning Approach. 通过p53热点突变进行癌症分类：一种集成学习方法。

IF 3.3 3区生物学

Cell Biology International Pub Date : 2025-05-30 DOI: 10.1002/cbin.70041

Manisha R Patil, Anand Bihari

{"title":"Cancer Classification Through p53 Hotspot Mutations: An Ensemble Learning Approach.","authors":"Manisha R Patil, Anand Bihari","doi":"10.1002/cbin.70041","DOIUrl":"https://doi.org/10.1002/cbin.70041","url":null,"abstract":"Tumor suppressor protein p53 is attracting a lot of attention in cancer research because of its role in both tumor cell survival and apoptosis. The most frequently altered tumor suppressor gene in human cancer is TP53. TP53 mutations affecting residues in the protein's DNA binding domain (102-292) account for 80% of the alterations detected in tumors. These are called hotspot mutations because they lose their wild-type function and acquire oncogenic functions that accelerate cancer progression. These functions include promoting the growth, migration, invasion, and initiation of cancer cells and granting drug resistance to cancer cells. Six residues of the p53 protein (Arg175, Gly245, Arg249, Arg248, Arg273, and Arg282) are often altered in human cancer, known as hotspot mutations. Based on these hotspot codons, we identified the cancer types and stability of protein p53 in this study. This study aims to classify cancer types with a high degree of accuracy and precision. The main contribution of this study is that our work presented mutation data (clinically and biologically meaningful features and the role of hotspot codon of protein p53) to classify types of cancer by learning from the labeled data using an ensemble approach. Our research on the classification of cancer types outperformed using the Extreme Gradient boosting classifier (XGBoost) with an accuracy of 99.85%, precision of 99.80%, area under the curve of 100%, MCC of 99. 85%, and F1 of 99.80%.","PeriodicalId":9806,"journal":{"name":"Cell Biology International","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144181010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harnessing Stem Cells for Pediatric Respiratory Diseases: Potential and Clinical Translation. 利用干细胞治疗儿科呼吸系统疾病：潜力和临床转化。

IF 3.3 3区生物学

Cell Biology International Pub Date : 2025-05-30 DOI: 10.1002/cbin.70042

Fatemeh Alizadeh, Sanaz Yasrebinia

引用次数: 0

Berberine Enhances the Sensitivity of Colorectal Cancer Cells to 5-FU Through Smoothing Endoplasmic Reticulum Stress-Mediated Autophagic Flux. 小檗碱通过平滑内质网应激介导的自噬通量增强结直肠癌细胞对5-FU的敏感性

IF 3.3 3区生物学

Cell Biology International Pub Date : 2025-05-30 DOI: 10.1002/cbin.70038

Junyu Xu, Min Hu, Ying Li, Huiming Gong, Xiaoyan Zhang, Ziyue He, Chi Xiao, Chengzhong Yang, Jun Zeng

{"title":"Berberine Enhances the Sensitivity of Colorectal Cancer Cells to 5-FU Through Smoothing Endoplasmic Reticulum Stress-Mediated Autophagic Flux.","authors":"Junyu Xu, Min Hu, Ying Li, Huiming Gong, Xiaoyan Zhang, Ziyue He, Chi Xiao, Chengzhong Yang, Jun Zeng","doi":"10.1002/cbin.70038","DOIUrl":"https://doi.org/10.1002/cbin.70038","url":null,"abstract":"Berberine (BBR), one of the main active isoquinoline alkaloids in Coptis chinensis, has gradually gained attention for its therapeutic effect on various tumors, including colorectal cancer (CRC). However, the detailed mechanisms underlying remain to be elucidated. The cytotoxic potential of BBR towards CRC cells was examined by MTT. Autophagy was indicated by acidic vesicular organelle formation, LC3 dots accumulation and conversion of LC3I to LC3II, while autophagic flux was monitored by the fusion of autophagosomes and lysosomes based on green fluorescence quenching in cells transfected with mRFP-GFP-LC3 plasmids and P62 degration. Furthermore, endoplasmic reticulum (ER) stress response-associated proteins in CRC cell lines treated with BBR and/or ER stress inhibitor 4-Phenylbutyric acid (4-BPA) were assessed by Western blotting. BBR-induced intracellular ROS accumulation was measured by DCFH-DA. ZIP Synergy scores were calculated using Synergyfinder software to evaluate the synergistic effects of BBR and 5-fluorouracil (5-FU). BBR induced cell death by autophagy-dependent mechanisms and resulted in sustained ER stress and oxidative stress, which contributed to the induction of complete autophagic flux. The inhibition of autophagy by chloroquine (CQ) partially reversed the anticancer effect of BBR, suggesting that BBR induced cytotoxic autophagy. Importantly, BBR enhanced the sensitivity of CRC cells to 5-FU.BBR might be a novel chemotherapy adjuvant drug that targets colorectal cancer by regulating ER stress/oxidative stress/autophagy/apoptosis.","PeriodicalId":9806,"journal":{"name":"Cell Biology International","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144180848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Disruption of Retriever Function Impacts Retrograde Trafficking From Endosomes. 寻回功能的破坏影响内体的逆行运输。

IF 3.3 3区生物学

Cell Biology International Pub Date : 2025-05-29 DOI: 10.1002/cbin.70037

Zebin Li, Zhe Yang, Rohan D Teasdale

{"title":"Disruption of Retriever Function Impacts Retrograde Trafficking From Endosomes.","authors":"Zebin Li, Zhe Yang, Rohan D Teasdale","doi":"10.1002/cbin.70037","DOIUrl":"https://doi.org/10.1002/cbin.70037","url":null,"abstract":"Within endosomes cargo proteins are sorted and packaged into endosomal-transport carriers (ETCs) enabling their delivery to other intracellular compartments. Retromer, a conserved multimeric protein complex, has defined functions in sorting cargo mediating formation of ETCs for both retrograde trafficking back to the trans-Golgi network (TGN) and recycling of cargo to the cell surface. Recent studies have identified the retriever complex, which is structurally like retromer, that also can function in the recycling of cargo from endosomes. However, retriever's function in retrograde trafficking from endosomes has not been investigated. CrispR mediated knock-out cell models for retromer and retriever in A549 lung carcinoma epithelial cells were generated. Retriever's role in recycling of established cargo Integrin β1 in A549 cells was confirmed. Cation-independent mannose 6-phosphate receptor and TGN46, two well-established retrograde cargos showed a redistribution from the TGN to early endosomes in retromer knockout (KO) and retriever KO cells which is consistent with decreased retrograde trafficking. Application of a ETC redirection assay in A549 cells identified that ETCs dependent on either retromer or retriever can be tethered by Golgin97 and Golgin245, but not GCC88. Overall, the presence of retriever is required for efficient endosomal retrograde trafficking. Within A549 cells, the requirement of retromer for Wiskott-Aldrich Syndrome Protein and SCAR Homolog (WASH) endosomal recruitment was confirmed while recruitment of WASH in the absence of retriever was not reduced. Furthermore, the efficient recruitment of retriever to endosomes was dependent on retromer. The underlying mechanism by which these complexes initiate the formation of retrograde ETCs therefore seems distinct.","PeriodicalId":9806,"journal":{"name":"Cell Biology International","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144172931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flavokawain A Attenuated Chronic Kidney Disease: Evidence From Network Pharmacology and Experimental Verification. 黄卡温A减毒慢性肾病：来自网络药理学和实验验证的证据。

IF 3.3 3区生物学

Cell Biology International Pub Date : 2025-05-26 DOI: 10.1002/cbin.70036

Yugant Krishnakumar Talati, Neha Dagar, Anil Bhanudas Gaikwad

{"title":"Flavokawain A Attenuated Chronic Kidney Disease: Evidence From Network Pharmacology and Experimental Verification.","authors":"Yugant Krishnakumar Talati, Neha Dagar, Anil Bhanudas Gaikwad","doi":"10.1002/cbin.70036","DOIUrl":"https://doi.org/10.1002/cbin.70036","url":null,"abstract":"Chronic kidney disease (CKD) is a silent global epidemic affecting ~700 million people worldwide, contributing to rising mortality rates. Despite the variety of underlying causes, renal fibrosis is the key pathological feature of CKD. Flavokawain A (FKA), a natural chalcone, is thought to offer protective effects against CKD through its anti-inflammatory, antioxidant, and anti-fibrotic properties. This study aims to investigate the therapeutic potential of FKA against CKD, using network pharmacology (NP), molecular docking analysis, and In Vivo validation. GeneCards, SwissTargetPrediction, and SuperPred databases were utilized to identify therapeutic targets related to CKD and FKA. Protein-protein interactions (PPIs) were performed using the STRING database. Gene ontology and pathway enrichment analyses were performed with DAVID databases, followed by network construction in Cytoscape. For validation, molecular docking studies were performed using PyRx and tested at doses of 50 mg/kg and 100 mg/kg (p.o.) for 21 days using a unilateral ureteral obstruction (UUO) rat model. The study identified 109 therapeutic targets for FKA in relation to CKD, highlighting 11 hub targets and 78 potential pathways. Molecular docking showed strong binding efficacy with nuclear factor κB subunit 1 (NF-κB1) and matrix metallopeptidase 9 (MMP9). In vivo validation supported these findings, as FKA administration showed protective effects on kidney function and histology with the downregulation of extracellular matrix (ECM) markers, such as fibronectin (FN) and transforming growth factor β1 (TGF-β1), along with reduced expression of NF-κB1 and MMP9. These findings indicate that FKA could be a valuable therapeutic candidate for managing CKD by targeting NF-κB1 and MMP9.","PeriodicalId":9806,"journal":{"name":"Cell Biology International","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144149457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

USP39 Depletion Plays Repressive Roles in Laryngeal Squamous Cell Carcinoma Development. USP39缺失在喉部鳞状细胞癌发展中起抑制作用

IF 3.3 3区生物学

Cell Biology International Pub Date : 2025-05-22 DOI: 10.1002/cbin.70035

Wen Xie, Lei Zhang, Xiaoyan Hu, Yahua Zhong, Zheng Li

{"title":"USP39 Depletion Plays Repressive Roles in Laryngeal Squamous Cell Carcinoma Development.","authors":"Wen Xie, Lei Zhang, Xiaoyan Hu, Yahua Zhong, Zheng Li","doi":"10.1002/cbin.70035","DOIUrl":"https://doi.org/10.1002/cbin.70035","url":null,"abstract":"Laryngeal squamous cell carcinoma (LSCC) is a prevalent malignant tumor of the upper respiratory tract. Ubiquitin-specific protease 39 (USP39) has been identified as an oncogenic regulator in various malignant tumors; however, its specific roles in LSCC remain unexplored. In this study, immunohistochemistry was employed to assess USP39 expression in LSCC tissues and adjacent normal tissues. Subsequently, a USP39 knockdown cellular model was established to investigate its effects on cell proliferation, apoptosis, and migration through Celigo cell counting, colony formation, flow cytometry, and transwell assays, respectively. A tumor-bearing animal model was established to verify the impact of USP39 on LSCC In Vivo. Co-immunoprecipitation (Co-IP) assay was used to validate protein-protein interaction. Our results suggested that USP39 was highly expressed in laryngeal cancer tissues, which exhibited a correlation with lymphatic metastasis. In Vitro loss-of-function experiments revealed that depletion of USP39 suppressed cell proliferation and migration, and induced apoptosis in LSCC cells. Furthermore, silencing USP39 restrained tumor growth silencing USP39 In Vivo. Mechanistically, USP39 was found to interact with and upregulated Aurora kinase B (AURKB). AURKB depletion attenuated the protumorigenic effects of USP39 overexpression. Additionally, USP39 enhanced ERK1/2 phosphorylation, and pharmacological inhibition of ERK1/2 abrogated USP39-driven proliferative and clonogenic capacities. In summary, this study underscores the significance of USP39 in the development of LSCC, positioning it as a promising therapeutic target for LSCC treatment.","PeriodicalId":9806,"journal":{"name":"Cell Biology International","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cyclophosphamide Induces Glioblastoma Tumor Cell Death and Oxidative Stress Through the Increase of TRPM2 Channel Stimulation: The Role of Carvacrol. 环磷酰胺通过增加TRPM2通道刺激诱导胶质母细胞瘤细胞死亡和氧化应激：香芹酚的作用。

IF 3.3 3区生物学

Cell Biology International Pub Date : 2025-05-22 DOI: 10.1002/cbin.70039

Kemal Ertilav, Mustafa Nazıroğlu

{"title":"Cyclophosphamide Induces Glioblastoma Tumor Cell Death and Oxidative Stress Through the Increase of TRPM2 Channel Stimulation: The Role of Carvacrol.","authors":"Kemal Ertilav, Mustafa Nazıroğlu","doi":"10.1002/cbin.70039","DOIUrl":"https://doi.org/10.1002/cbin.70039","url":null,"abstract":"Cyclophosphamide (CP) damages glioblastoma cells by producing an excessive amount of intracellular (iROS) and mitochondrial (mROS) reactive oxygen species. Both iROS and mROS are produced when TRPM2 is activated, but they are decreased when carvacrol (CAR) and N-(p-amylcinnamoyl) anthranilic acid (ACA) inhibit it. Therefore, iROS, and mROS via upregulating Ca2+ influx and apoptosis in glioblastoma (DBTRG-05MG) cells, CP-mediated TRPM2 stimulation may cause oxidant and apoptotic activities. We investigated how TRPM2 activation not only promotes DBTRG-05MG death but also modifies oxidative damage and apoptosis to counteract the effects of ACA and CAR. The groups of control (CN), CAR (200 μM for 24 h), CP (2 mM for 24 h), and CP + CAR were induced in the DBTRG-05MG. While cytosolic free Ca2+ levels decreased in the cells as a result of the CAR and ACA treatments, they were further elevated in the CP group by the stimulation of TRPM2 (H2O2). The cells in the CP group had higher levels of dead cell percentage, apoptosis, mitochondrial membrane dysfunction, mROS, iROS, and caspases -3, -8, and -9 than the CN and CAR cells, although their levels were lower in the CP + CAR than in the CP only. CAR incubation increased the CP-induced glutathione concentration and cell viability percentage declines. In summary, the anticancer effect of CP was enhanced by TRPM2 stimulation, while CP-induced oxidative stress and DBTRG-05MG death were reduced by TRPM2 suppression when CAR was treated. TRPM2 activation may be a possible tumor killer channel due to oxidative glioma damage caused by CP.","PeriodicalId":9806,"journal":{"name":"Cell Biology International","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144118959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Ultrastructure of the Apical Organ of the Goette's Larvae of the Polyclad Flatworm Stylochus pilidium Indicates Homology Between Polyclad Larvae. 多包覆扁虫毛茎柱头幼虫顶端器官超微结构的研究表明多包覆幼虫具有同源性。

IF 3.3 3区生物学

Cell Biology International Pub Date : 2025-05-15 DOI: 10.1002/cbin.70034

Davina Düngler, Clemens Gotsis, Isabel L Dittmann, Stefan Redl, Michael W Hess, Bernhard Egger

{"title":"The Ultrastructure of the Apical Organ of the Goette's Larvae of the Polyclad Flatworm Stylochus pilidium Indicates Homology Between Polyclad Larvae.","authors":"Davina Düngler, Clemens Gotsis, Isabel L Dittmann, Stefan Redl, Michael W Hess, Bernhard Egger","doi":"10.1002/cbin.70034","DOIUrl":"https://doi.org/10.1002/cbin.70034","url":null,"abstract":"Polyclad flatworms exhibit both direct and indirect development, with various larval types observed, including Müller's larva, Kato's larva, Goette's larva and Curini-Galletti's larva. The different larval types are distinguished by shape, number of eyes and number of lobes. The Goette's larva of Stylochus pilidium exhibits a distinct six-lobed morphology, with one cerebral and one epidermal eye. The posterior half of the larva features a posterior tuft and four lobes, two of which are ventrolateral and two of which are lateral. Anteriorly, a larger lobe called the oral hood is situated ventrally, opposite a smaller dorsal lobe. The larval types share a distinct feature, known as the apical organ, which is located at the anterior tip of the larvae. Here, we investigated the ultrastructure of the apical organ in Goette's larvae of the polyclad S. pilidium. Six apical tuft sensory (ATS) cells are at the centre of the apical organ, encircled by a ring of apical tuft gland (ATG) cell type 1 necks. These cell necks merge into two distinct gland cells that extend dorsoposteriorly and terminate posterior to the brain. Two epidermal apical tuft anchor (ATAn) cells encircle the ATS cells and the central gland cell necks. Additionally, four ATG cell type 2 necks, which are distributed symmetrically around the ATAn cells, merge into a single cell and extend ventrally to the level of the cerebral eye. The third type of ATG cells is in a circular pattern around the anchor cells, with necks in the epidermal layer. The ultrastructural arrangements of the apical organ in Goette's larva of S. pilidium are very similar to those of previously studied polyclad larvae, supporting the hypothesis of a common origin of larvae within Polycladida.","PeriodicalId":9806,"journal":{"name":"Cell Biology International","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144076293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulatory T Cells in Tumor Microenvironment: Therapeutic Approaches and Clinical Implications. 肿瘤微环境中的调节性T细胞：治疗方法和临床意义。

IF 3.3 3区生物学

Cell Biology International Pub Date : 2025-05-14 DOI: 10.1002/cbin.70031

Niti Sureka, Sufian Zaheer

引用次数: 0

ω-3 PUFAs Alleviate GJ-Cx43 Uncoupling Induced by Long-Term Isoflurane Exposure by Activating the Wnt/β-catenin Signaling Pathway in Astrocytes. ω-3 PUFAs通过激活星形胶质细胞Wnt/β-catenin信号通路缓解长期异氟醚暴露诱导的GJ-Cx43解偶联

IF 3.3 3区生物学

Cell Biology International Pub Date : 2025-05-13 DOI: 10.1002/cbin.70028

Zimo Wang, Rui Dong, Yuqiang Han, Liangyu Peng, Shuai Liu, Zhengliang Ma, Tianjiao Xia, Xiaoping Gu

引用次数: 0