Cellular and molecular biology最新文献_第9页

Engineering erucic acid biosynthesis in camelina (Camelina sativa) via FAE1 gene cloning and antisense technology. 通过 FAE1 基因克隆和反义技术实现荠菜（Camelina sativa）中芥酸的生物合成。

IF 1.5 4区生物学

Cellular and molecular biology Pub Date : 2024-07-28 DOI: 10.14715/cmb/2024.70.7.35

Hoda Bashiri, Danial Kahrizi, Ali Hatef Salmanian, Hassan Rahnama, Pejman Azadi

{"title":"Engineering erucic acid biosynthesis in camelina (Camelina sativa) via FAE1 gene cloning and antisense technology.","authors":"Hoda Bashiri, Danial Kahrizi, Ali Hatef Salmanian, Hassan Rahnama, Pejman Azadi","doi":"10.14715/cmb/2024.70.7.35","DOIUrl":"10.14715/cmb/2024.70.7.35","url":null,"abstract":"Oil seeds now make up the world's second-largest food source after cereals. In recent years, the medicinal- oil plant Camelina sativa has attracted much attention for its high levels of unsaturated fatty acids and low levels of saturated fatty acids as well as its resistance to abiotic stresses. Improvement of oil quality is considered an important trait in this plant. Erucic acid is one of the fatty acids affecting the quality of camelina oil. Altering the fatty acid composition in camelina oil through genetic manipulation requires the identification, isolation, and cloning of genes involved in fatty acid biosynthesis. The Fatty Acid Elongase 1 (FAE1) gene encodes the enzyme β-ketoacyl CoA synthase (KCS), a crucial enzyme in the biosynthesis of erucic acid. In this study, the isolation and cloning of the FAE1 gene from Camelina sativa were conducted to construct an antisense structure. The molecular homology modeling of DFAE1 proteins using the SWISS-MODEL server on ExPASy led to the generation of the 3D structures of FAE1 and DFAE1 proteins. The GMQE values of 0.44 for FAE1 and 0.08 for DFAE1 suggest high accuracy in the structural estimation of these genes. The fragments were isolated from the DNA source of the genomic Soheil cultivar with an erucic acid content of about 3% (in matured seeds) using PCR. After cloning the FAE1 gene into the Bluescript II SK+ vector and sequencing, the resulting fragments were utilized to construct the antisense structure in the pBI121 plant expression vector. The approved antisense structure was introduced into the Camelina plant using the Agrobacterium-mediated method, with optimization of tissue culture and gene transfer conditions. This approach holds potential to advance our knowledge of fat biosynthesis, leading to potential improvements in oil quality in Camelina sativa.","PeriodicalId":9802,"journal":{"name":"Cellular and molecular biology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141888577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prenatal methamphetamine hydrochloride exposure downregulates miRNA-151-3p and CACNA1C in testis rats' offspring. 产前接触盐酸甲基苯丙胺会下调大鼠后代睾丸中的 miRNA-151-3p 和 CACNA1C。

IF 1.5 4区生物学

Cellular and molecular biology Pub Date : 2024-07-28 DOI: 10.14715/cmb/2024.70.7.31

Romina Bakhshaei, Nayere Zare, Zahra Baghi Zadeh, Batool Ghorbani Yekta

引用次数: 0

Pan-cancer analysis of TMEM45A and exploration of its prognostic value and mechanism in gastric cancer. TMEM45A的泛癌分析及其在胃癌中的预后价值和机制探讨

IF 1.5 4区生物学

Cellular and molecular biology Pub Date : 2024-07-28 DOI: 10.14715/cmb/2024.70.7.32

Qixin Xie, Tao Guo, Hong Deng, Changjun Yu, Changyi Fang

{"title":"Pan-cancer analysis of TMEM45A and exploration of its prognostic value and mechanism in gastric cancer.","authors":"Qixin Xie, Tao Guo, Hong Deng, Changjun Yu, Changyi Fang","doi":"10.14715/cmb/2024.70.7.32","DOIUrl":"10.14715/cmb/2024.70.7.32","url":null,"abstract":"Cancer is a major category of diseases that need to be addressed urgently, bringing a huge burden to the world. Gastric cancer (GC) is a frequent malignant tumor of the digestive system with the highest incidence and mortality rate among all tumors. The purpose of this study was to explore the mechanism of action of TMEM45A in pan-cancer and gastric cancer. First, GEO and TCGA database were employed to analyze the expression of TMEM45A in GC patients. Then, we determined the association between TMEM45A expression and survival of GC patients using the Kaplan-Meier Plotter database and TCGA database and verified the accuracy of TMEM45A in predicting prognosis. Next, we analyzed the effect of CTHRC expression on TIICs in GC tissues. A prognostic model was constructed using immunomodulatory genes associated with TMEM45A. The specificity and accuracy of the model were verified. TMEM45A expression was markedly higher in GC tissue than in normal tissue. GC patients with TMEM45A overexpression had a poor prognosis. The AUC value of 5-year survival on the ROC curve was 0.705, indicating that TMEM45A is a reliable prognostic factor and can be used as a clinicopathological indicator alone to predict patient prognosis. Three high-risk immunomodulatory genes (CXCR4 and TGFB1) and one low-risk immunomodulatory gene (PDCD1) were obtained using both univariate and multivariate COX methods. These three immunomodulatory molecules were used to construct prognostic models. GC patients with TMEM45A overexpression have a poor prognosis and are associated with immune cell infiltration. Hence, TMEM45A is a fairly reliable independent prognostic marker.","PeriodicalId":9802,"journal":{"name":"Cellular and molecular biology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141888616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Huangbaiye Tuji combined with Longdan Xiegan Decoction for anal cryptitis: Analysis of clinical efficacy and its influence on disease recurrence. 黄柏叶土茯苓汤联合龙胆泻肝汤治疗肛隐窝炎：临床疗效分析及其对疾病复发的影响

IF 1.5 4区生物学

Cellular and molecular biology Pub Date : 2024-07-28 DOI: 10.14715/cmb/2024.70.7.17

Lin Yang, Jiazhen Liu, Jingyang Liang, Yan Wang, Lu Liu

引用次数: 0

Cytogenetic study of Meriz goat breeds in Iraqi Kurdistan region. 伊拉克库尔德斯坦地区梅里兹山羊品种的细胞遗传学研究。

IF 1.5 4区生物学

Cellular and molecular biology Pub Date : 2024-07-28 DOI: 10.14715/cmb/2024.70.7.22

Dilger Maghded Khdhr, Karim Jalal Karim

{"title":"Cytogenetic study of Meriz goat breeds in Iraqi Kurdistan region.","authors":"Dilger Maghded Khdhr, Karim Jalal Karim","doi":"10.14715/cmb/2024.70.7.22","DOIUrl":"https://doi.org/10.14715/cmb/2024.70.7.22","url":null,"abstract":"Goats are considered the leading farm animal that has a substantial role in the agricultural sector in the Kurdistan Region of Iraq. No cytological examination has been carried out on them. This experiment aims to identify the Karyotype of the local breeds of domestic goats. This experiment was conducted on the Karyotype and prepared the ideogram of Meriz goats. The determination of the relative length and centromeric index arm ratio of the chromosomes in the breed was achieved by the production of karyotypes. A total of (30)Meriz goats, consisting of (10) males and (20) females, were selected to collect blood samples for a short-term lymphocyte culture. The diploid chromosome count was observed to be (60), consisting of (29) pairs of acrocentric autosomes and one pair of allosomes, specifically the X and Y chromosomes. The acrocentric nature of the X-chromosome and the sub-metacentric nature of the Y-chromosome were identified through scientific investigation. The study observed a variation in the relative length of autosomal chromosomes in Meriz goats, with females ranging from 4.49% to 1.89% and males ranging from (4.53%) to (1.75%). The X-chromosome had a relative length of 3.96 in females, while the Y-chromosome displayed a relative length of (5.05). The findings of this karyological investigation suggest that the chromosomal composition seen in the Meriz goats under examination was within the expected range of normalcy. It is recommended that more cytogenetic analyses be conducted at the population level in order to identify individuals within the Meriz breed population who possesses numerical and/or structural chromosome abnormalities. This research is crucial for enhancing the efficiency of production and reproduction in this breed.","PeriodicalId":9802,"journal":{"name":"Cellular and molecular biology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141888549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic and morphological diversity of introduced cultivars of almonds (Prunus amygdalus L.) in Bosnia and Herzegovina. 波斯尼亚和黑塞哥维那引进的杏仁（Prunus amygdalus L.）栽培品种的遗传和形态多样性。

IF 1.5 4区生物学

Cellular and molecular biology Pub Date : 2024-07-28 DOI: 10.14715/cmb/2024.70.7.15

Jasna Hasanbegovic, Semina Hadizabulic, Mirsad Kurtović, Fuad Gaši, Sezai Ercisli, Boris Dorbić, Melekber Sulusoglu Durul

{"title":"Genetic and morphological diversity of introduced cultivars of almonds (Prunus amygdalus L.) in Bosnia and Herzegovina.","authors":"Jasna Hasanbegovic, Semina Hadizabulic, Mirsad Kurtović, Fuad Gaši, Sezai Ercisli, Boris Dorbić, Melekber Sulusoglu Durul","doi":"10.14715/cmb/2024.70.7.15","DOIUrl":"https://doi.org/10.14715/cmb/2024.70.7.15","url":null,"abstract":"The main morphological and genetic characterization of seven introduced almond cultivars in Bosnia & Herzegovina was conducted. The almond cultivars included three from Italy (Tuono, Genco, Supernova), two from France (Ferragnes and Ferraduel), and two from the USA (Texas and Nonpareil). Genetic characterization was utilized by using 10 microsatellite markers, with nine markers from Prunus persicae and one from Prunus armeniaca. The results of genetic characterization revealed an average of 5.40 alleles per primer per locus. The average number of effective alleles for the 10 SSR loci of introduced cultivars was 3.92. The Shannon Information Index averaged 1.41. The observed heterozygosity (Ho) and expected heterozygosity (He) averaged 0.53 and 0.69, respectively. Morphological analyses of the fruit of introduced almond cultivars in Bosnia & Herzegovina indicated favorable agroecological conditions for their cultivation and spread. The results suggest that these introduced almond cultivars could be utilized in breeding programs to enhance the genetic diversity of the local almond population in Bosnia & Herzegovina.","PeriodicalId":9802,"journal":{"name":"Cellular and molecular biology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141888583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Idebenone protects the kidneys of rats with renovascular arterial hypertension by inhibiting oxidative stress and apoptosis. 艾地苯醌可通过抑制氧化应激和细胞凋亡来保护新血管性动脉高血压大鼠的肾脏。

IF 1.5 4区生物学

Cellular and molecular biology Pub Date : 2024-07-28 DOI: 10.14715/cmb/2024.70.7.26

Jinsen He, Lixin Huang, Linqi Lu

{"title":"Idebenone protects the kidneys of rats with renovascular arterial hypertension by inhibiting oxidative stress and apoptosis.","authors":"Jinsen He, Lixin Huang, Linqi Lu","doi":"10.14715/cmb/2024.70.7.26","DOIUrl":"https://doi.org/10.14715/cmb/2024.70.7.26","url":null,"abstract":"Here, the protective effect of antioxidant Idebenone (IDB) on renovascular hypertension was studied. The two-kidney one-clip (2K-1C) model of renal hypertension was established. The rats were divided into 3 groups: sham-operation group, 2K-1C renal hypertensive rats' model group and model treated with IDB group. The mean arterial blood pressure (MBP) of rats was measured and pathological condition of kidney was observed by H&E staining. The change of renal damage biomarkers (Cre, BUN, urine proteins), inflammatory factors (IL-6, IL-1β and TNF-α), oxidative stress ratio and key factors (MDA, SOD and CAT) were assessed by kits. The apoptosis key proteins (BAD, BAX, Caspase9, GSK-3β) were detected via Western blot. The 2K-1C model of renal hypertension was established. IDB reduced the MBP, Cre, BUN, urine proteins and improved the pathological condition of 2K-1C kidney. IDB restrained the inflammation factors (IL-6, IL-1β and TNF-α) and oxidative stress in kidney of renal hypertensive rats' model. Besides, IDB suppressed the expression of apoptosis key factors (BAD, BAX, Caspase9, GSK-3β) in kidney of renal hypertensive rats' model. IDB protects the kidneys of rats with renovascular arterial hypertension by inhibiting inflammation, oxidative stress, and apoptosis. These findings might provide medication guidance for IDB in renovascular arterial hypertension.","PeriodicalId":9802,"journal":{"name":"Cellular and molecular biology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141888609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

99mTc-HYNIC-Annexin V detection of apoptosis in the rabbit model of liver cancer. 99mTc-HYNIC-Annexin V 检测兔肝癌模型中的细胞凋亡。

IF 1.5 4区生物学

Cellular and molecular biology Pub Date : 2024-07-28 DOI: 10.14715/cmb/2024.70.7.20

Zuoliang Shi, Mengcheng Hu, Jianmin Bian

{"title":"99mTc-HYNIC-Annexin V detection of apoptosis in the rabbit model of liver cancer.","authors":"Zuoliang Shi, Mengcheng Hu, Jianmin Bian","doi":"10.14715/cmb/2024.70.7.20","DOIUrl":"10.14715/cmb/2024.70.7.20","url":null,"abstract":"To investigate the feasibility of detection of apoptosis in vivo by 99mTc-HYNIC-Annexin V, Annexin V was labeled with 99mTc through HYNIC. 18 New Zealand rabbits implanted VX-2 were randomly divided into control (n = 8) and paclitaxel (PAC, n = 10) groups, given 2 mL/kg of normal saline or 2.4 mg/kg of PAC intravenously. The liver tumor imaging was detected by SPECT through intravenous injection of 99mTc-HYNIC-Annexin V before treatment, 24 hours and 48 hours after treatment respectively. Tumor radioactive count proportion to non-tumor sites was calculated. When the last imaging was finished, the rabbits were sacrificed. The tumor was taken out and divided into two pieces, one for TUNEL immunohistochemical analysis and the other for flow cytometry (FCM). We found that the rate of Annexin V labeled with 99mTc through HYNIC was more than 95%, and radiochemical purity was above 95%. The SPECT showed that two groups had no significant tumor imaging before the treatment. There is no significant tumor imaging in control group, while the PAC group 24 h and 48 h after treatment showed significant accumulation. The Tumor/non-Tumor (T/NT) in PAC group at 24 h and 48 h after chemotherapy was significantly different from that in the control group and PAC group prior to treatment. There was no significant difference between 24 h and 48 h in PAC group. The TUNEL-positive cells detected by immunohistochemistry and apoptotic rate detected by FCM in PAC group were significant different from those in control group. The T/NT was significantly correlated to TUNEL-positive cells and apoptotic rate of the tumor. PAC can induce apoptosis of rabbit VX-2 liver cancer cells. 24-48 h after paclitaxel chemotherapy is a window time for apoptosis detection. Apoptotic cells in vivo can be detected by SPECT through 99mTc-HYNIC-Annexin V.","PeriodicalId":9802,"journal":{"name":"Cellular and molecular biology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141888544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression and prognostic analysis of PFKFB4 in oral squamous cell carcinoma. 口腔鳞状细胞癌中 PFKFB4 的表达和预后分析

IF 1.5 4区生物学

Cellular and molecular biology Pub Date : 2024-07-28 DOI: 10.14715/cmb/2024.70.7.28

Shuting Chen, Jiusong Han, Wei Wang, Zhengzheng Wu, Juan Wang, Bin Cheng

{"title":"Expression and prognostic analysis of PFKFB4 in oral squamous cell carcinoma.","authors":"Shuting Chen, Jiusong Han, Wei Wang, Zhengzheng Wu, Juan Wang, Bin Cheng","doi":"10.14715/cmb/2024.70.7.28","DOIUrl":"10.14715/cmb/2024.70.7.28","url":null,"abstract":"Fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase 4 (PFKFB4) is a crucial enzyme in the glycolysis pathway, possessing both kinase and phosphatase capabilities. Although it has emerged as an important oncogene in various cancer types, its function in oral squamous cell carcinoma (OSCC) is still not well understood. In our research, PFKFB4 expression was assessed via immunohistochemical (IHC) staining of tissue microarrays and OSCC patient specimens. The transcriptional expression of PFKFB4 in OSCC was analyzed by utilizing The Cancer Genome Atlas (TCGA) dataset. Correlation between PFKFB4 expression and clinicopathological features was examined using the χ2 test. Prognostic investigation of PFKFB4 was conducted via Kaplan-Meier and Cox analyses. PFKFB4 levels were notably elevated in OSCC samples in comparison to adjacent normal tissues (P < 0.001). Elevated PFKFB4 expression was associated with higher histologic grade (P = 0.0438), higher T stage (P = 0.031), and more advanced clinical stage (P = 0.0063). The ROC curve demonstrated the diagnostic potential of PFKFB4 (AUC = 0.827). Increased levels of PFKFB4 were linked to decreased overall survival (OS) (P = 0.04), poorer disease-specific survival (DSS) (P = 0.04), and shorter progression-free interval (PFI) (P < 0.001). PFKFB4 expression was identified as an independent risk factor for OS based on Cox regression analysis [hazard ratio (HR) = 1.517, P = 0.044)]. An OS nomogram was constructed with a concordance index of 0.690. Our findings reveal that upregulated PFKFB4 expression in OSCC tissues could serve as a potential prognostic biomarker.","PeriodicalId":9802,"journal":{"name":"Cellular and molecular biology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141888582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heterozygous variants in transmembrane channel-like 1 gene cause autosomal recessive nonsyndromic hearing loss. 跨膜通道样 1 基因的杂合子变异导致常染色体隐性非综合征性听力损失。

IF 1.5 4区生物学

Cellular and molecular biology Pub Date : 2024-07-28 DOI: 10.14715/cmb/2024.70.7.19

Lan Zhang, Qingwen Zhu, Yanzhao Wu, Ping Shi

{"title":"Heterozygous variants in transmembrane channel-like 1 gene cause autosomal recessive nonsyndromic hearing loss.","authors":"Lan Zhang, Qingwen Zhu, Yanzhao Wu, Ping Shi","doi":"10.14715/cmb/2024.70.7.19","DOIUrl":"10.14715/cmb/2024.70.7.19","url":null,"abstract":"Autosomal recessive non-syndromic hearing loss (ARNSHL) can cause severe or very severe pre-speech hearing loss. Transmembrane channel-like 1 (TMC1) gene is the sixth deafness gene discovered, but the precise extent of its protein structure and function is unknown. First, history collection, audiology examination and imaging examination were performed on the proband and his family members. Peripheral blood of proband and family members was collected, genomic DNA was extracted, exon high-throughput sequencing technology was used to detect the deafness gene mutation of the proband, and Sanger sequencing was performed to verify the TMC1 gene of the proband's parents. The proband was born with hearing impairment, normal tympanic function, inability to induce acoustic reflex in both ears (acoustic reflex threshold is 100 dBHL), and severe sensorineural deafness. One of his sisters has severe sensorineural hearing loss, and neither his parents nor his other sister is hearing impaired. High-throughput sequencing of the proband identified mutations at c.741+3_741+6delAAGT (splicing) and c.884C>T (p.A295V) of the TMC1 gene, two of which were heterozygous mutations. Sanger sequencing confirmed that the c.884C > T mutation was inherited from the mother, while the c.741+3_741+6delAAGT mutation was derived from the father. Prediction of amino acid function suggested that both mutations were pathogenic mutations. In conclusion, we found a new pathogenic complex heterozygous mutation of the TMC1 gene, which enriched the mutation spectrum of the TMC1 gene and provided a basis for genetic counseling and prenatal diagnosis of ARNSHL.","PeriodicalId":9802,"journal":{"name":"Cellular and molecular biology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141888586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0