Cellular and molecular biology最新文献_第10页

The role of remifentanil in regulating mitochondrial autophagy in osteoclasts was investigated based on PINK1/Parkin pathway. 基于 PINK1/Parkin 通路，研究了瑞芬太尼在调节破骨细胞线粒体自噬中的作用。

IF 1.5 4区生物学

Cellular and molecular biology Pub Date : 2024-07-28 DOI: 10.14715/cmb/2024.70.7.27

Pufeng Ye, Guifeng Pan, Yuanfeng Li, Aobo Li, Jianbin Zhang, Mingxiu Xin, Zhenjiang Mai

{"title":"The role of remifentanil in regulating mitochondrial autophagy in osteoclasts was investigated based on PINK1/Parkin pathway.","authors":"Pufeng Ye, Guifeng Pan, Yuanfeng Li, Aobo Li, Jianbin Zhang, Mingxiu Xin, Zhenjiang Mai","doi":"10.14715/cmb/2024.70.7.27","DOIUrl":"10.14715/cmb/2024.70.7.27","url":null,"abstract":"This study aimed to explore the regulatory effect of remifentanil-mediated mitochondrial autophagy on osteoclast formation and further investigate its mechanism. Macrophage cell line RAW264.7 was taken and induced to differentiate into mature osteoclasts using nuclear factor kB receptor activating factor ligand (RANKL). The cell model was treated with different concentrations of remifentanil or down-regulated expression of mitochondrial autophagy-related gene PINK1. The survival, death and ROS production of osteoclasts were detected by CCK8 kit and flow cytometry, MMP level was detected by JC-1 method, mitochondrial morphology and autophagy were observed by transmission electron microscopy, and mitochondrial autophagy-related protein expression was detected by Western blot. The number of osteoclasts in the remifentanil-treated group was significantly reduced compared to the control group, accompanied by a reduction in reactive oxygen species (ROS) and mitochondrial membrane potential levels (MMP). Further results showed that remifentanil could significantly up-regulate the activity of PINK1/Parkin pathway, promote the occurrence of mitochondrial autophagy, and damaged mitochondria, and inhibit the formation of osteoclasts. Remifentanil successfully inhibited osteoclast formation by regulating mitochondrial autophagy mediated by PINK1/Parkin pathway. The results of this study revealed that remifentanil plays an important role in the physiology and pathology of osteoclasts, which may provide new ideas and strategies for the clinical treatment of remifentanil in tibial fractures.","PeriodicalId":9802,"journal":{"name":"Cellular and molecular biology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141888589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Goosecoid promotes pancreatic adenocarcinoma metastasis through TGF-β signaling. 鹅膏通过TGF-β信号促进胰腺癌转移

IF 1.5 4区生物学

Cellular and molecular biology Pub Date : 2024-07-28 DOI: 10.14715/cmb/2024.70.7.14

Yong Meng, Rui Li, Weirong Jiang, Wenhao Chen, Zhiwen Li, Zhen Xu, Shuaiming Geng

{"title":"Goosecoid promotes pancreatic adenocarcinoma metastasis through TGF-β signaling.","authors":"Yong Meng, Rui Li, Weirong Jiang, Wenhao Chen, Zhiwen Li, Zhen Xu, Shuaiming Geng","doi":"10.14715/cmb/2024.70.7.14","DOIUrl":"10.14715/cmb/2024.70.7.14","url":null,"abstract":"Goosecoid (GSC), translated from a homeobox gene, is a protein that participates in metastasis of various cancers. Pancreatic adenocarcinoma (PAAD) is one of the deadliest malignancies associated with a poor diagnosis and prognosis. To develop new treatment target or biomarker for PAAD, this study intended to assess the effects and the molecular mechanism of GSC on PAAD metastasis. The expressive discrepancy of GSC in PAAD and normal tissues/cells was compared by both the quantitative PCR and western blot. The effects of GSC silencing and GSC over-expression on PAAD cells and TGF-β signaling were proved by wound-healing assay, cell counting kit-8, Transwell assay and western blot. From the results, GSC mRNA and protein levels were enriched in PAAD cancer tissues and cells. GSC silencing prohibited metastasis of PAAD cells including the ability to invade, migrate and epithelial-mesenchymal transition (EMT), whereas GSC upregulation stimulated these cells behaviors above. GSC silencing reversed the effects on cellular processes induced by activation of the TGF-β pathway. Furthermore, silencing of GSC postponed tumor growth in xenograft model. In summary, GSC was abundantly expressed in PAAD, which activated the TGF-β pathway to enhance cell metastasis and tumor development.","PeriodicalId":9802,"journal":{"name":"Cellular and molecular biology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141888585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing rooting tobacco (Nicotiana tabacum) plant by loaded indole-3-butyric acid in alginate/chitosan nanocapsule. 在海藻酸/壳聚糖纳米胶囊中添加吲哚-3-丁酸，促进烟草（Nicotiana tabacum）植株生根。

IF 1.5 4区生物学

Cellular and molecular biology Pub Date : 2024-07-28 DOI: 10.14715/cmb/2024.70.7.34

Masoumeh Ghorbani, Danial Kahrizi, Elham Arkan, Farank Aghaz, Alireza Zebarjadi

{"title":"Enhancing rooting tobacco (Nicotiana tabacum) plant by loaded indole-3-butyric acid in alginate/chitosan nanocapsule.","authors":"Masoumeh Ghorbani, Danial Kahrizi, Elham Arkan, Farank Aghaz, Alireza Zebarjadi","doi":"10.14715/cmb/2024.70.7.34","DOIUrl":"https://doi.org/10.14715/cmb/2024.70.7.34","url":null,"abstract":"Recently, nanocarriers have been utilized for encapsulating and sustained release of agrochemicals specifically auxins. Due to their potential applications such as increased bioavailability and improved crop yield and nutritional quality. Herein, the efficacy of alginate/chitosan nanocapsules as a nanocarrier for the hormone indole-3-butyric acid (IBA) loading and its effect on rooting tobacco plants has been carried out in the present study. The average particle size of IBA-alginate/chitosan nanocapsules was measured by Dynamic light scattering analysis at 321 nm. Scanning electron microscope studies revealed the spherical shape of nanoparticles with an average size of 97 nm. The average particle size of IBA-alginate/chitosan nanocapsules was measured by Dynamic light scattering analysis at 321 nm. The characteristic peaks of IBA on alginate/chitosan nanocapsules were identified by Fourier transform infrared spectroscopic analysis. Also, high efficiency (35%) of IBA hormone loading was observed. The findings indicated that the concentration of 3 mgL-1 of IBA-alginate/chitosan nanocapsules has the highest efficiency in increasing the rooting in tobacco (Nicotiana tabacum) plants compared to other treatments. According to our results, we can introduce alginate/chitosan nanocapsules as an efficient nanocarrier in IBA hormone transfer applications and their use in agriculture.","PeriodicalId":9802,"journal":{"name":"Cellular and molecular biology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141888578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CRISPR-Cas9 Mediated AGO2 Knockout inhibits tumorigenesis in human colorectal cancer cells. CRISPR-Cas9 介导的 AGO2 基因敲除抑制了人类结直肠癌细胞的肿瘤发生。

IF 1.5 4区生物学

Cellular and molecular biology Pub Date : 2024-07-28 DOI: 10.14715/cmb/2024.70.7.25

Yizhi Zhu, Gengfang Wang, Haoran Xu, Yuan Guo

{"title":"CRISPR-Cas9 Mediated AGO2 Knockout inhibits tumorigenesis in human colorectal cancer cells.","authors":"Yizhi Zhu, Gengfang Wang, Haoran Xu, Yuan Guo","doi":"10.14715/cmb/2024.70.7.25","DOIUrl":"10.14715/cmb/2024.70.7.25","url":null,"abstract":"AGO2 plays a vital role in small RNA-guided gene silencing, which has been implied in the tumorigenesis of different types of tumors. Fundamentally, increased expression of AGO2 protein is associated with cancer progression and metastasis. This study aims to investigate the molecular mechanism by which AGO2 promotes tumorigenesis in colorectal cancer (CRC). Databases were used to analyze the expression levels of AGO2 in CRC and confirmed by a quantitative reverse transcriptase-PCR (qRT-PCR) assay in CRC tissues and normal adjacent tissues collected from 25 CRC patients. CRISPR/Cas9-mediated genome editing was used to knockout the AGO2 in HCT116 cells as a model system for colorectal cancers. The cell proliferation, migration and invasion ability of HCT116 cells were detected by CCK-8 assay, Wound scratch assay and Transwell assay. Moreover, the quantities of miRNA binding with AGO2 were detected by RNA-Binding Protein Immunoprecipitation (RIP-Assay). We demonstrated that AGO2 was aberrantly high-expressed in 25 matched-tissue pairs of colorectal cancer and para-carcinoma tissue. The following functional experiments verified that knockout of AGO2 suppressed cell proliferation, migration and tumorigenesis to hamper the aggressiveness of CRC. Our study also suggests a possible link between AGO2 and miRNA in RISC. AGO2 was elevated in CRC and knockout of AGO2 suppressed proliferation and tumorigenicity of CRC cells. Moreover, RISC formation and the function of miRNAs are also subject to AGO2. AGO2 may be a meaningful target for CRC therapy.","PeriodicalId":9802,"journal":{"name":"Cellular and molecular biology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141888548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The fibroblast heterogeneity across keloid, normal and tumor samples from single-cell resolution. 从单细胞分辨率观察瘢痕疙瘩、正常和肿瘤样本中成纤维细胞的异质性。

IF 1.5 4区生物学

Cellular and molecular biology Pub Date : 2024-07-28 DOI: 10.14715/cmb/2024.70.7.29

Xia Wang, Weihu Huang, Yongtie Li, Chen Zhu

{"title":"The fibroblast heterogeneity across keloid, normal and tumor samples from single-cell resolution.","authors":"Xia Wang, Weihu Huang, Yongtie Li, Chen Zhu","doi":"10.14715/cmb/2024.70.7.29","DOIUrl":"https://doi.org/10.14715/cmb/2024.70.7.29","url":null,"abstract":"Keloids are defined as a benign dermal fibroproliferative disorder, with excessive fibroblast proliferation, and excessive overproduction of collagen. Although the heterogeneity during keloid development has been extensively studied, the heterogeneity across different skin states is still unclear. So, a global comparison across skin states is needed. In this study, we collected samples from 5 states of skin, including melanoma, cutaneous squamous cell carcinoma, keloid skin, scar skin, and healthy control samples. The heterogeneity of cell types and subtypes was analyzed and compared across 5 states, and we observed significant differences among them. Our results showed a cancer-like fibroblast, which is not in normal samples, may play an important role in antigen processing and presentation. We also noticed that the mesenchymal fibroblast increased in keloid samples, which highly expressed POSTN. And POSTN may participate in epithelial-mesenchymal transition and collagen overexpression to promote keloid growth. These findings help to understand the alteration among different skin states and provide potential genetic basis for keloid therapies.","PeriodicalId":9802,"journal":{"name":"Cellular and molecular biology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141888620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic analysis of SARS-CoV-2 spike gene using Next Generation Sequencing from COVID-19 patients in Erbil/Iraq. 利用新一代测序技术对伊拉克埃尔比勒 COVID-19 患者的 SARS-CoV-2 穗基因进行遗传分析。

IF 1.5 4区生物学

Cellular and molecular biology Pub Date : 2024-06-05 DOI: 10.14715/cmb/2024.70.6.2

Asma Ameen Ghareeb, Sazan Moffaq Abdulaziz

{"title":"Genetic analysis of SARS-CoV-2 spike gene using Next Generation Sequencing from COVID-19 patients in Erbil/Iraq.","authors":"Asma Ameen Ghareeb, Sazan Moffaq Abdulaziz","doi":"10.14715/cmb/2024.70.6.2","DOIUrl":"10.14715/cmb/2024.70.6.2","url":null,"abstract":"SARS-CoV-2 has been identified by the WHO as a new virus causing mild to severe respiratory illnesses that belong to the Coronavirus family. The virus underwent rapid and continuous changes in the genetic material, especially the S gene, during COVID-19 pandemic and generated a number of new variants announced by WHO in late 2020. Mutations in the S gene have greatly affected virus pathogenesis as the spike protein is responsible for many critical processes. Delta and Omicron variants were studied extensively due to increased mortality and morbidity rates associated with their pandemic waves. This study aimed to analyse the S gene through NGS in an attempt to identify and characterize the circulating variants among the infected population in Erbil/Iraq. Nasopharyngeal and throat swab samples were collected from hospitalized and non-hospitalized patients with COVID-19 symptoms in Erbil City/Iraq from the 1st of November 2021 to the 28th of February 2022. Following confirmation of SARS-CoV-2 infection by RT-PCR, 15 samples were selected and sent to Intergen Lab (Ankara/Turkey) for NGS and analysis. Following analysis and alignment of the received sequences with the Wuhan-Hu-1 strain (wild-type), Delta variant was identified in 13 samples, and Omicron in two. On the whole, different mutation classes have been observed including nonsynonymous, synonymous, non-frameshift deletions and a non-frameshift insertion. The Delta-specific set of mutations, L452R, T478K and P681R, was detected in all Delta isolates. Both Omicron variants appeared to have 35 mutations. D614G variation was conserved in both variants.","PeriodicalId":9802,"journal":{"name":"Cellular and molecular biology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141247249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Growth inhibition of subcutaneous tumor with glioma cells in nude mice by silencing TLR4. 通过沉默 TLR4 抑制裸鼠皮下胶质瘤细胞的生长。

IF 1.5 4区生物学

Cellular and molecular biology Pub Date : 2024-06-05 DOI: 10.14715/cmb/2024.70.6.32

Zhansen An, Cuihong Zheng, Liang Liu, Zefeng He, Xuan Yang, Jing Liu, Jing Tong, Yingzi Liu

{"title":"Growth inhibition of subcutaneous tumor with glioma cells in nude mice by silencing TLR4.","authors":"Zhansen An, Cuihong Zheng, Liang Liu, Zefeng He, Xuan Yang, Jing Liu, Jing Tong, Yingzi Liu","doi":"10.14715/cmb/2024.70.6.32","DOIUrl":"10.14715/cmb/2024.70.6.32","url":null,"abstract":"This study investigated the regulatory impact of Toll-like receptor 4 (TLR4) gene on glioma cell proliferation and apoptosis, elucidating the molecular mechanisms underlying TLR4-induced growth inhibition in vivo. U-87MG-Sh and U-87MG-NC cells, with silenced TLR4 and negative control plasmid respectively, were established. Eighteen nude mice, divided into transfection, negative control, and blank control groups, were inoculated with corresponding cells. Over four weeks, the transfection group exhibited significantly reduced tumor growth rates, smaller mass and volume, and lower growth activity compared to controls. Histological analysis revealed sparse tumor cells, increased fibrous connective tissue, and slower angiogenesis in the transfection group. Flow cytometry demonstrated a lower proliferation index and increased G0/1 cell count in the transfection group. mRNA levels of TLR4, NF-κB, and CyclinD1 were significantly lower in the transfection group. TLR4 silencing correlated with U-87MG cell proliferation regulation, growth inhibition, NF-κB and CyclinD1 modulation, and induction of cell cycle arrest and apoptosis. These findings suggest TLR4 as a potential gene therapy target for glioma.","PeriodicalId":9802,"journal":{"name":"Cellular and molecular biology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141247309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of omega-3 fatty acid supplementation on markers of inflammation and endothelial function in patients with chronic heart disease: A systematic review and meta-analysis. 补充欧米伽-3 脂肪酸对慢性心脏病患者炎症指标和内皮功能的影响：系统回顾和荟萃分析。

IF 1.6 4区生物学

Cellular and molecular biology Pub Date : 2024-06-05 DOI: 10.14715/cmb/2024.70.6.26

Mohammed Ibrahim Mohialdeen Gubari

{"title":"Effect of omega-3 fatty acid supplementation on markers of inflammation and endothelial function in patients with chronic heart disease: A systematic review and meta-analysis.","authors":"Mohammed Ibrahim Mohialdeen Gubari","doi":"10.14715/cmb/2024.70.6.26","DOIUrl":"10.14715/cmb/2024.70.6.26","url":null,"abstract":"Chronic heart disease (CHD) is still a major global cause of morbidity and mortality, necessitating effective therapeutic interventions to mitigate its progression. Omega-3 fatty acids (FAs) have garnered attention for their potential anti-inflammatory and endothelial-protective properties in CHD management. The present study aims to assess the efficacy of Omega-3 FA supplementation on markers of inflammation and endothelial function in patients with CHD. To achieve this, we used the relevant keywords to search international databases (Web of Science, PubMed, Embase, and Scopus) and extract publications evaluating the effectiveness of omega-3 FA supplementation on inflammation markers and endothelial function in patients with CHD. STATA (version 15) and the random and fixed-effects models were used to evaluate the collected data. Thirteen clinical trial studies met inclusion criteria, with a total sample size of 853 individuals (406 cases and 447 controls). The cases had a mean age of 58 ± 10.3 years. The pooled results indicated that omega-3 Omega-3 FA supplementation significantly reduced the level of circulating IL-6 (SMD = -0.47, 95% CI -1.29 to 0.35, %, p < 0.001), hs-CRP (SMD = -0.21, 95% CI -0.70 to 0.28, p = 0.01), and TNF-α (SMD = -0.56, 95% CI -1.14 to 0.01, p < 0.001) in patients with CHD. Also, findings revealed that a daily supplement of omega-3 significantly increased FMD by 0.34% (95% CI: 0.14-0.54%, p < 0.001) as compared with placebo by a fixed-effect model in patients with CHD. These findings underscore the potential therapeutic utility of omega-3 fatty acid supplementation in modulating inflammation and endothelial dysfunction in patients with CHD.","PeriodicalId":9802,"journal":{"name":"Cellular and molecular biology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141247210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The impact of laparoscopic radical gastrectomy on the inflammatory response and immune function of patients with gastric cancer. 腹腔镜胃癌根治术对胃癌患者炎症反应和免疫功能的影响。

IF 1.6 4区生物学

Cellular and molecular biology Pub Date : 2024-06-05 DOI: 10.14715/cmb/2024.70.6.15

Jie Ling, Xiangjun Wang, Junfeng Ying

{"title":"The impact of laparoscopic radical gastrectomy on the inflammatory response and immune function of patients with gastric cancer.","authors":"Jie Ling, Xiangjun Wang, Junfeng Ying","doi":"10.14715/cmb/2024.70.6.15","DOIUrl":"https://doi.org/10.14715/cmb/2024.70.6.15","url":null,"abstract":"The currest study aimed to measure the effects of laparoscopic radical gastrectomy on inflammatory response along with immune function in gastric cancer (GC) patients. Seventy patients with GC in our hospital were retrospectively chosen to be the study objects and separated into control group (CG, 35 cases) and observation group (OG, 35 cases). Patients in the OG received radical laparotomy. Patients in the OG received laparoscopic radical gastrectomy. The surgical indicators, postoperative recovery indicators, inflammatory factors, immune function, incidence of adverse reactions along with quality of life of patients in both groups were compared. In contrast to the CG, the operation time of the OG presented as shorter (P<0.05), and the amount of intraoperative blood loss together with postoperative VAS score in the OG presented lower (P<0.05), but the number of lymph nodes dissection presented not statistically significant between 2 groups (P>0.05). The postoperative exhaust time, feeding time as well as hospital stay in the OG presented shorter relative to the CG (P<0.05). The serum levels of CRP, and IL-6 together with TNF-α presented elevated in both groups after surgery, and those in the OG presented lower when compared with the CG (P<0.05). The serum levels of IgA, and IgG together with IgM presented declined in both groups after surgery, and those in the OG presented higher when compared with the CG (P<0.05). The incidence of postoperative complications in the OG presented reduction relative to the CG (P<0.05). The GLQI scores of the OG presented significantly higher relative to the CG at discharge (P<0.05). Compared with radical gastrectomy, laparoscopic radical gastrectomy is more suitable for the treatment of GC, which can reduce the inflammatory response and promote the immune function of GC patients.","PeriodicalId":9802,"journal":{"name":"Cellular and molecular biology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141247600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunohistochemical co-expression of PAX2 and CAIX predicts better prognosis in clear cell renal cell carcinoma after nephrectomy: A retrospective observational study. PAX2和CAIX的免疫组化共同表达可预测肾切除术后透明细胞肾细胞癌的预后：一项回顾性观察研究。

IF 1.5 4区生物学

Cellular and molecular biology Pub Date : 2024-06-05 DOI: 10.14715/cmb/2024.70.6.20

Honggang Shao, Yougang Liao, Min Xiang, Deng Hu, Sha Liu

{"title":"Immunohistochemical co-expression of PAX2 and CAIX predicts better prognosis in clear cell renal cell carcinoma after nephrectomy: A retrospective observational study.","authors":"Honggang Shao, Yougang Liao, Min Xiang, Deng Hu, Sha Liu","doi":"10.14715/cmb/2024.70.6.20","DOIUrl":"10.14715/cmb/2024.70.6.20","url":null,"abstract":"Clear cell renal cell carcinoma (ccRCC) is a lethal malignancy with high metastatic probability. Paired box 2 gene product (PAX2) carbonic anhydrase IX were biomolecules closely linked with ccRCC development and outcomes of multiple malignancies. We aim to explore the role of immunohistochemical staining of PAX2 and CAIX to predict ccRCC prognosis after nephrectomy. Surgical specimens of patients who were pathologically diagnosed as ccRCC were reviewed. Expression levels of PAX2 and CAIX were assessed via immunohistochemical staining. Recurrence-free survival (RFS) and overall survival were compared among different phenotypes. Inverse probability of treatment weighting (IPTW) was used for adjustment of confounding factors. 56 patients were included. Patients with PAX2 and CAIX high-expression (the two-high group, n=8) had significantly longer RFS and OS than those of simultaneously down-expression (the two-low group, n=31). Median RFS was 38.4 (95% CI: 32.3-NA) for the two-high group and 14.8 (95% CI: 13.4-39.0) months for the two-low group (P=0.043). IPTW confirmed PAX2 and CAIX co-expression is associated with less recurrence risk HR: 0.39, 95% CI: 0.17-0.92, P=0.031). Co-expression of PAX2 and CAIX is associated better prognosis of ccRCC. We are looking for validation by large cohort studies.","PeriodicalId":9802,"journal":{"name":"Cellular and molecular biology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141247320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0