Cellular and molecular biology最新文献_第7页

Hypoxia-inducible factor-1α/vascular endothelial growth factor signaling pathway-based ulcer-healing mechanism of Astragalus Aqueous extract in diabetic foot rats. 黄芪水提取物基于缺氧诱导因子-1α/血管内皮生长因子信号通路的糖尿病足大鼠溃疡愈合机制

IF 1.5 4区生物学

Cellular and molecular biology Pub Date : 2024-07-28 DOI: 10.14715/cmb/2024.70.7.11

Xuxu Jia, Juan Yang, Qian Guo, Honggang Ni, Yujie Yu, Jingrun Dai, Mei Jiang

{"title":"Hypoxia-inducible factor-1α/vascular endothelial growth factor signaling pathway-based ulcer-healing mechanism of Astragalus Aqueous extract in diabetic foot rats.","authors":"Xuxu Jia, Juan Yang, Qian Guo, Honggang Ni, Yujie Yu, Jingrun Dai, Mei Jiang","doi":"10.14715/cmb/2024.70.7.11","DOIUrl":"10.14715/cmb/2024.70.7.11","url":null,"abstract":"The main objective of this work was to investigate the mechanism of Astragalus aqueous extract ulcer healing in diabetic foot model rats through the hypoxia-inducible factor 1-alpha (HIF-1ɑ)/vascular endothelial growth factor (VEGF) signalling pathway. Fifty specific-pathogen-free male Sprague Dawley rats were divided into blank (A), model control (B), Astragalus extract (C) and mupirocin (D) treatment groups. Group A received a regular diet, whereas the other groups received a high-fat/high-sugar diet and intraperitoneal streptozotocin injections to induce diabetes. Diabetic foot ulcers were created via skin excision. Subsequently, ulcers were debrided daily. Groups B, C and D received wet saline gauze, wet gauze with Astragalus extract and gauze with mupirocin, respectively, on the affected area. Group A received no treatment. After 14 days, the rats were assessed for ulcer healing and general condition. Immunohistochemistry was used to detect HIF-1ɑ and VEGF levels in the dorsalis pedis artery, and ELISA was used to determine serum IL-6 and CRP levels. The results revealed that Groups C and D had significantly faster ulcer healing compared with Group B (p < 0.01), and ulcer healing was faster in Group C than in Group D (p < 0.01). Group C exhibited notably higher HIF-1ɑ and VEGF protein expression levels compared with Groups B and D (p < 0.01). IL-6 and CRP expression levels in Groups C and D were significantly lower than those in Group B (p < 0.01). In summary, Astragalus aqueous extract effectively treats diabetic foot ulcers by up-regulating HIF-1ɑ and VEGF expression, activating the HIF-1ɑ/VEGF pathway, improving local tissue ischaemia and hypoxia, promoting collateral circulation and enhancing dorsalis pedis artery formation, thereby accelerating ulcer repair in diabetic rats.","PeriodicalId":9802,"journal":{"name":"Cellular and molecular biology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141888588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Linggui Zhugan decoction as a potential medicine for neuroprotection in Alzheimer's Disease via AMPK pathway. 灵桂术甘煎剂是通过 AMPK 通路保护阿尔茨海默病神经的潜在药物

IF 1.5 4区生物学

Cellular and molecular biology Pub Date : 2024-07-28 DOI: 10.14715/cmb/2024.70.7.23

Yun Fan, Yun Ling, Jiulve Hu, Zijun Hou, Runpeng Dou, Chunxiang Zhou

{"title":"Linggui Zhugan decoction as a potential medicine for neuroprotection in Alzheimer's Disease via AMPK pathway.","authors":"Yun Fan, Yun Ling, Jiulve Hu, Zijun Hou, Runpeng Dou, Chunxiang Zhou","doi":"10.14715/cmb/2024.70.7.23","DOIUrl":"10.14715/cmb/2024.70.7.23","url":null,"abstract":"Alzheimer's disease (AD) is a degenerative dementia illness that causes atrophy of the temporal and frontal lobes of the cerebral cortex. Linggui Zhugan (LGZG), a classic Chinese herbal formula, was initially recognized as a safe and effective treatment of cardiovascular diseases for long history. This study intended to assess the effects and the molecular mechanism of LGZG on AD progress. C57BL/6 mice were divided into six groups: normal mice, amyloid precursor protein/presenilin 1 (APP/PS1) mice (model group), positive control group (model mice treated with donepezil), high, medium and low LGZG group (model mice treated with 7g/kg/d, 3.5g/kg/d or 1.75g/kg/d LGZG respectively). Water maze results showed that the escape latency and path length of high and medium LGZG groups declined compared to the model mice, the decline degree was dose-dependent. The hippocampal slices of six groups were analyzed by Nissl-staining, Perls' iron staining and immunofluorescence assay. The results indicated LGZG could restore morphological anomalies and alleviate iron deposition of AD mice, and the GXP4 positive cells increased significantly. The MDA, Fe2+ and GSH were measured by biochemical testing, whose results illustrated that LGZG could normalize MDA, Fe2+ and GSH levels in AD model compared to un-treated APP/PS1 model. The higher dose of LGZG the mice received, the more intensive effects on those levels of molecules. Western blot results showed that LGZG could affect NeuN, AMPK, p53, SLC7A11 and GPX4 levels in the hippocampus of AD model, which was all proteins related to AMPK pathway. In conclusion, LGZG has a neuroprotective effect on AD through AMPK pathway by alleviating oxidative stress and ferroptosis.","PeriodicalId":9802,"journal":{"name":"Cellular and molecular biology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141888610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MiR-3195 inhibits non-small cell lung cancer malignant behaviors along with cisplatin resistance through targeting PFKFB4. MiR-3195 通过靶向 PFKFB4 抑制非小细胞肺癌的恶性行为和顺铂耐药性。

IF 1.5 4区生物学

Cellular and molecular biology Pub Date : 2024-07-28 DOI: 10.14715/cmb/2024.70.7.10

Rong Chen, Xiaoyan Gao, Yahui Shen

{"title":"MiR-3195 inhibits non-small cell lung cancer malignant behaviors along with cisplatin resistance through targeting PFKFB4.","authors":"Rong Chen, Xiaoyan Gao, Yahui Shen","doi":"10.14715/cmb/2024.70.7.10","DOIUrl":"10.14715/cmb/2024.70.7.10","url":null,"abstract":"Chemotherapy presents the main therapy of non-small cell lung cancer (NSCLC). Nevertheless, cisplatin-based therapy can be limited by drug resistance. MicroRNA (miRNA) possesses a vital regulatory function in modulating the progression as well as cisplatin resistance of NSCLC, but how miR-3195 influences NSCLC is obscure. In this work, it was discovered that miR-3195 presented definite down-regulation in NSCLC cells. Gain-of function assays revealed that overexpressing miR-3195 hindered NSCLC cell proliferation together with migration whereas induced cell apoptosis. Mechanically, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 (PFKFB4) presented the target gene of miR-3195 and was high-expressed in NSCLC cells. The repressive impacts of overexpressing miR-3195 on NSCLC cells malignant behaviors were reversed via PFKFB4 elevation. Additionally, elevated miR-3195 expression reduced cisplatin resistance of NSCLC both in vitro as well as in vivo. PFKFB4 elevation could offset the reduced cisplatin resistance caused by miR-3195 overexpression in NSCLC cells. In conclusion, this work clarified miR-3195 repressed NSCLC cell proliferation, migration, as well as cisplatin resistance by modulating PFKFB4. Our study might provide a promising clue to promote the anti-tumor effects of chemotherapy.","PeriodicalId":9802,"journal":{"name":"Cellular and molecular biology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141888611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pulsatillic acid as a potential inhibitor of protein kinase C-alpha in non-small cell lung cancer. 作为非小细胞肺癌蛋白激酶 C-α 潜在抑制剂的普鲁卡因酸

IF 1.5 4区生物学

Cellular and molecular biology Pub Date : 2024-07-28 DOI: 10.14715/cmb/2024.70.7.7

Basiouny Basiouny El-Gamal, Thoraya A Elgader, Mohamed Abd Ellatif, Safaa Omer, Marwa Saeed, Muniera Mohieldeen, Ayyub A Patel, Arshi Malik, Refaat A Eid, Mohammed Amanullah, Awad S Alsamghan, Marya Ahsan, Ayaz Khurram Mallick

{"title":"Pulsatillic acid as a potential inhibitor of protein kinase C-alpha in non-small cell lung cancer.","authors":"Basiouny Basiouny El-Gamal, Thoraya A Elgader, Mohamed Abd Ellatif, Safaa Omer, Marwa Saeed, Muniera Mohieldeen, Ayyub A Patel, Arshi Malik, Refaat A Eid, Mohammed Amanullah, Awad S Alsamghan, Marya Ahsan, Ayaz Khurram Mallick","doi":"10.14715/cmb/2024.70.7.7","DOIUrl":"10.14715/cmb/2024.70.7.7","url":null,"abstract":"Non-small cell lung cancer (NSCLC) is a global health concern with a significant impact on morbidity and mortality. Small molecule inhibitors targeting genetic mutations like EGFR and ALK have shown promise in NSCLC treatment. This study focuses on Protein Kinase C-alpha (PKCα), implicated in NSCLC pathogenesis. Overexpression of PKCα correlates with advanced disease stages. Preclinical studies suggest its inhibition can suppress NSCLC cell growth. The research employs molecular docking to identify Pulsatillic acid (PA) as a potential PKCα inhibitor. ADMET predictions support PA's candidacy and PASS analysis and Swiss Target Prediction reveal its biological properties. Fluorescence-based binding assays demonstrate PA's inhibitory potency on PKCα, aligning with molecular docking findings. Cytotoxicity assays show PA's minimal impact on HEK-293 cell viability, with an IC50 of 21.03 μM in A549 cells. mRNA expression analysis in A549 cells indicates PA's potential inhibitory effect on PKCα. In conclusion, this study highlights that PA may emerge as a promising therapeutic candidate for NSCLC, emphasizing the need for further research, validation, and exploration of its translational potential. The study contributes valuable insights into NSCLC treatment strategies, emphasizing the significance of targeting PKCα.","PeriodicalId":9802,"journal":{"name":"Cellular and molecular biology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141888619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioinformatics analysis of high-intensity intermittent exercise for prevention of myocardial infarction. 高强度间歇运动预防心肌梗死的生物信息学分析。

IF 1.5 4区生物学

Cellular and molecular biology Pub Date : 2024-07-28 DOI: 10.14715/cmb/2024.70.7.13

Shihua Tan, Chen Lin, Huarui Li, Fenglin Peng

{"title":"Bioinformatics analysis of high-intensity intermittent exercise for prevention of myocardial infarction.","authors":"Shihua Tan, Chen Lin, Huarui Li, Fenglin Peng","doi":"10.14715/cmb/2024.70.7.13","DOIUrl":"https://doi.org/10.14715/cmb/2024.70.7.13","url":null,"abstract":"The mechanism of target interaction involving high-intensity interval training (HIIT) in improving prognosis of myocardial infarction (MI) remains unclear. This study aimed to establish a visual network of \"HIIT-target-disease\" by referring to the methods of pharmacological disease and drug bioinformatic analysis, to explore the potential targets, and key targets and predict the potential biological mechanism of high-intensity intermittent exercise in preventing and treating myocardial infarction. Public data resources such as OMIM, NCBI and GeneCards were used to find potential targets of high-intensity intermittent exercise and myocardial infarction. Key targets of overlap between exercise and disease were determined according to the Relevance score values analyzed by GeneCards. The visual network diagram of \"HIIT - Multi-target-disease\" was constructed by Cytoscape. A total of 4820 disease targets and 528 high-intensity intermittent exercise targets were screened out, and 444 overlapped targets were obtained, including 425 protein targets. Five core protein targets were selected: IL10, PPARA, TNF, IL6, and STAT3. It may pass PI3K-AKT signaling pathway, Insulin resistance pathway, T-cell signaling pathway, TNF signaling pathway, and JAX-STAT signaling pathway and other pathways play a role. Our study comprehensively elucidated the potential targets, key targets and molecular mechanisms of high-intensity intermittent exercise in improving the prognosis of myocardial infarction, and proved that high-intensity intermittent exercise can act on multiple targets and multiple pathways to play a good preventive and therapeutic effect on myocardial infarction, providing scientific theoretical basis for revealing the mechanism of high-intensity intermittent exercise in the prevention and treatment of cardiovascular disease.","PeriodicalId":9802,"journal":{"name":"Cellular and molecular biology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141888546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epigenetics modification among vitrified oocytes and early embryos derived from them: a narrative review. 玻璃化卵母细胞及其衍生早期胚胎的表观遗传学改变：综述。

IF 1.5 4区生物学

Cellular and molecular biology Pub Date : 2024-07-28 DOI: 10.14715/cmb/2024.70.7.4

Mostafa Saeedinia, Jiayi Chen, Omid Kohandel Gargari, Kyana Jafarabady, Majid Zaki-Dizaji, Alireza Ghorbankhanloo, Zohreh Heidary

引用次数: 0

Exploring the In Vitro and In Vivo Therapeutic Efficacy of Juniperus oxycedrus Cade Oil: Antioxidant, Anti-inflammatory, and Anti-asthmatic Effects in an Allergic Asthma Model. 探索桧油的体外和体内疗效：过敏性哮喘模型中的抗氧化、抗炎和抗哮喘效果。

IF 1.5 4区生物学

Cellular and molecular biology Pub Date : 2024-07-28 DOI: 10.14715/cmb/2024.70.7.8

Meryem Ahmida, Mustapha Hichem Zadam, Nesrine Djaber, Taha Khaldi, Chawki Bensouici, Latifa Khattabi, Hichem Amara, Moncef Zaafour, Amel Boumendjel, Mahfoud Messarah, Mahieddine Boumendjel

{"title":"Exploring the In Vitro and In Vivo Therapeutic Efficacy of Juniperus oxycedrus Cade Oil: Antioxidant, Anti-inflammatory, and Anti-asthmatic Effects in an Allergic Asthma Model.","authors":"Meryem Ahmida, Mustapha Hichem Zadam, Nesrine Djaber, Taha Khaldi, Chawki Bensouici, Latifa Khattabi, Hichem Amara, Moncef Zaafour, Amel Boumendjel, Mahfoud Messarah, Mahieddine Boumendjel","doi":"10.14715/cmb/2024.70.7.8","DOIUrl":"https://doi.org/10.14715/cmb/2024.70.7.8","url":null,"abstract":"This investigation aimed to explore the antioxidant, anti-inflammatory effects of Cade oil and its efficacy within a Wistar allergic asthma model. The antioxidant activity was assessed through various in vitro tests using chain-breaking antioxidant effects (radical scavenging and reducing abilities assays). In vivo experiments involved Wistar rats categorized into four groups: negative control group, Ovalbumin-sensitised/challenged group, Cade oil-treated group, and Ovalbumin-sensitised/challenged Cade oil-treated group. These experiments aimed to evaluate oxidative stress parameters in the lungs and erythrocytes. The results indicated that the Cade oil exhibited significant antioxidant capabilities, evidenced by its radical scavenging activity against DPPH, ABTS, and Galvinoxyl radicals, with IC50 values ranging from 21.92 to 24.44 µg/mL. Besides, the reducing abilities methods showed A0,5 value ranging from 11.51 to 30.40 µg/mL for reducing power, Cupric ion reducing antioxidant capacity, and O-phenanthroline assays. Additionally, the IC50 value for β-carotene scavenging was found to be (8.2 ± 0.25 µg/ml). Analysis revealed high levels of polyphenols and flavonoids in Cade oil, indicating rich polyphenol (275.21 ± 3.14 mg GAE/g DW) and flavonoid (28.23 ± 1.91 µg QE/mg) content. In vivo findings highlighted Cade oil's efficacy in reducing inflammatory cell recruitment, enhancing antioxidant status, reducing lipid peroxidation, and improving histopathological alterations within the allergic asthma model. These results demonstrated that Cade oil has a potent antioxidant, anti-inflammatory, and anti-asthmatic properties, suggesting its potential therapeutic application in asthma treatment.","PeriodicalId":9802,"journal":{"name":"Cellular and molecular biology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141888581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Network preservation analysis to identify transcriptional biomarkers related to flowering in Crocus sativus. 通过网络保存分析确定与番红花开花相关的转录生物标记。

IF 1.5 4区生物学

Cellular and molecular biology Pub Date : 2024-07-28 DOI: 10.14715/cmb/2024.70.7.9

Mahsa Eshaghi, Sajad Rashidi-Monfared

{"title":"Network preservation analysis to identify transcriptional biomarkers related to flowering in Crocus sativus.","authors":"Mahsa Eshaghi, Sajad Rashidi-Monfared","doi":"10.14715/cmb/2024.70.7.9","DOIUrl":"https://doi.org/10.14715/cmb/2024.70.7.9","url":null,"abstract":"Crocus sativus L. is known as an ornamental geophyte and a source of valuable spice and secondary metabolites. Network preservation module analysis is one of the best approaches to revealing special features of different conditions. It can determine patterns of divergence and conservation between transcriptome data. Herein, we explored the regulatory genes of the flowering process by RNA-Seq data containing flowering and non-flowering samples in gene expression profiles. Persevered module analysis revealed three significant non-persevered modules related to the flowering process, namely pink, green, and blue. Several hub genes associated with non-preserved modules such as PIA1, NAC90, ALY3, Sus3, MYB31, ARF5/MP, MYB31, HD-ZIP, SEP3d, OR_B, AGL6a, bZIP(TGA1) and GRAS were identified. These candidate genes can be considered key diagnostic biomarkers for the flowering process. Here, we also compared two approaches, WGCNA and NetRep for module preservation analysis. The results of these methods were consistent with non-preserved modules. NetRep was a faster (11 times) and more efficient (run more than 10000 permutations for each comparison) method than WGCNA module preservation. Differential expression genes (DEGs) screening showed that many hub genes were downregulated in non-flowering than flowering samples. Our finding revealed regulatory mechanisms of the flowering process in C. sativus as can be developed transcriptional biomarkers which could pave the way for promoting saffron yield via flowering induction.","PeriodicalId":9802,"journal":{"name":"Cellular and molecular biology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141888614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitofusin 1 and 2 overexpression reduces AβO-mediated ER stress and apoptosis in N2a APPswe cells. 过表达 Mitofusin 1 和 2 可减少 AβO 介导的 N2a APPswe 细胞ER应激和细胞凋亡。

IF 1.5 4区生物学

Cellular and molecular biology Pub Date : 2024-07-28 DOI: 10.14715/cmb/2024.70.7.2

Min Kyoung Kam, Su-Min Jung, Ga Eun Lee, Sung Woo Lee, Hong Jun Lee, Young-Ho Park, Dong-Seok Lee

{"title":"Mitofusin 1 and 2 overexpression reduces AβO-mediated ER stress and apoptosis in N2a APPswe cells.","authors":"Min Kyoung Kam, Su-Min Jung, Ga Eun Lee, Sung Woo Lee, Hong Jun Lee, Young-Ho Park, Dong-Seok Lee","doi":"10.14715/cmb/2024.70.7.2","DOIUrl":"10.14715/cmb/2024.70.7.2","url":null,"abstract":"Alzheimer's disease (AD) is the most common neurodegenerative disorder, and amyloid beta oligomers (AβO), which are pathological markers of AD, are known to be highly toxic. AβO increase mitochondrial dysfunction, which is accompanied by a decrease in mitochondrial fusion. Although mitofusin (Mfn) 1 and Mfn2 are mitochondrial fusion proteins, Mfn2 is known to regulate endoplasmic reticulum (ER) function, as it is located in the ER. Several studies have shown that AβO exacerbates ER stress, however, the exact mechanism requires further elucidation. In this study, we used mouse neuroblastoma cells stably overexpressing the amyloid precursor protein (APP) with the Swedish mutation (N2a APPswe cells) to investigate the role of Mfn in ER stress. Our results revealed that amyloid beta (Aβ) caused cellular toxicity in N2a APPswe cells, upregulated ER stress-related proteins, and promoted ER expansion. The AβO-mediated ER stress was reduced when Mfn1 and Mfn2 were overexpressed. Moreover, Mfn1 and Mfn2 overexpressed resulted in reduced apoptosis of N2a APPswe cells. In conclusion, our results indicate that both Mfn1 and Mfn2 reduce ER stress and apoptosis. Our data provide a foundation for future studies on the roles of Mfn1 and Mfn2 in the molecular mechanisms underlying AβO-mediated ER stress and the pathogenesis of AD.","PeriodicalId":9802,"journal":{"name":"Cellular and molecular biology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141888612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of XRCC2 with breast cancer, a multi-omics analysis at genomic, transcriptomic, and epigenomic level. 从基因组、转录组和表观基因组水平对 XRCC2 与乳腺癌的关系进行多组学分析。

IF 1.5 4区生物学

Cellular and molecular biology Pub Date : 2024-07-28 DOI: 10.14715/cmb/2024.70.7.36

Naser Gilani, Mehmet Ozaslan

{"title":"Association of XRCC2 with breast cancer, a multi-omics analysis at genomic, transcriptomic, and epigenomic level.","authors":"Naser Gilani, Mehmet Ozaslan","doi":"10.14715/cmb/2024.70.7.36","DOIUrl":"10.14715/cmb/2024.70.7.36","url":null,"abstract":"One of the main causes of cancer-related mortality for women worldwide is breast cancer (BC). The XRCC2 gene, essential for DNA repair, has been implicated in cancer susceptibility. This study aims to evaluate the association between XRCC2 and BC risk. The study was conducted at Zheen International Hospital in Erbil, Iraq, between 2021 and 2024 with a total of 88 samples, including 44 paired normal and cancer tissue samples. Mutation analysis was performed using Next-Generation Sequencing, coupled with in silico tools for variant impact prediction. Expression levels were assessed through RT-PCR, and methylation status was determined using methylation-sensitive restriction enzyme digestion PCR. The study identified seven inherited germline variants in the XRCC2 gene, with five of these mutations being Uncertain Significance, one being Likely Pathogenic, and one being Likely benign. RNA purity was found high with mean A260/280 ratios of 1.986 ± 0.097 in normal (N) and 1.963 ± 0.092 in tumor (T) samples. Tumor samples exhibited a higher RNA concentration (78.56 ± 40.87 ng/µL) than normal samples (71.44 ± 40.79 ng/µL). XRCC2 gene expression was significantly upregulated in tumor tissue, with marked increases in patients aged 40-55 and >56 years and in higher cancer grades (II and III) and invasive ductal carcinoma (p-values ranging from <0.0001 to 0.0392). DNA methylation rates in tumor tissues were low (7%), suggesting limited regulation by methylation. The study suggests that XRCC2 can be classified as an oncogene and that its structural investigation by targeted NGS and expression evaluation can be used as a potential biomarker in BC.","PeriodicalId":9802,"journal":{"name":"Cellular and molecular biology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141888545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0