Cell reports最新文献_第4页

Noncanonical phagocytosis-like SEAL establishes mammalian fertilization. 非典范吞噬样 SEAL 建立了哺乳动物的受精过程。

IF 7.5 1区生物学

Cell reports Pub Date : 2025-03-25 DOI: 10.1016/j.celrep.2025.115463

Naokazu Inoue, Takako Saito, Ikuo Wada

{"title":"Noncanonical phagocytosis-like SEAL establishes mammalian fertilization.","authors":"Naokazu Inoue, Takako Saito, Ikuo Wada","doi":"10.1016/j.celrep.2025.115463","DOIUrl":"https://doi.org/10.1016/j.celrep.2025.115463","url":null,"abstract":"In many forms of sexual reproduction, only the most robust spermatozoa, which overcome multiple physiological challenges, reach the oocyte. However, the exact mechanisms of gamete recognition and fusion are unknown. In the present study, we demonstrated that with the onset of gamete recognition, oocyte microvilli form lamellipodium-like structures, activate actin polymerization, and subsequently engulf spermatozoa to initiate gamete fusion. Gamete fusion occurred via a phagocytosis-like process we termed \"sperm engulfment activated by IZUMO1-JUNO linkage and gamete fusion-related factors\" (SEAL). Gamete adhesion was strictly regulated by binding of sperm IZUMO1 to oocyte JUNO, while SEAL was primarily mediated by sperm DCST1/2, SPACA6, TMEM95, FIMP, and TMEM81, the essential factors for gamete fusion. Interestingly, JUNO was almost depleted from oocyte surfaces in the region where SEAL enveloped spermatozoa by microvilli without actin polymerization. SEAL formation was recapitulated using JUNO-expressing K562 lymphocytic cells rather than oocytes. Together, these findings suggest that dynamic rearrangement of membrane components facilitates SEAL prior to successful fertilization.","PeriodicalId":9798,"journal":{"name":"Cell reports","volume":"44 4","pages":"115463"},"PeriodicalIF":7.5,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143728980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NUCLEAR RNA POLYMERASE D1 is essential for tomato embryogenesis and desiccation tolerance in seeds.

IF 7.5 1区生物学

Cell reports Pub Date : 2025-03-25 Epub Date: 2025-02-20 DOI: 10.1016/j.celrep.2025.115345

Yixuan Feng, Yiming Wang, Tai Wang, Lingtong Liu

引用次数: 0

Hexokinase 2-mediated metabolic stress and inflammation burden of liver macrophages via histone lactylation in MASLD.

IF 7.5 1区生物学

Cell reports Pub Date : 2025-03-25 Epub Date: 2025-02-25 DOI: 10.1016/j.celrep.2025.115350

Jinyang Li, Xiancheng Chen, Shiyu Song, Wangjie Jiang, Tianjiao Geng, Tiantian Wang, Yan Xu, Yongqiang Zhu, Jun Lu, Yongxiang Xia, Rong Wang

{"title":"Hexokinase 2-mediated metabolic stress and inflammation burden of liver macrophages via histone lactylation in MASLD.","authors":"Jinyang Li, Xiancheng Chen, Shiyu Song, Wangjie Jiang, Tianjiao Geng, Tiantian Wang, Yan Xu, Yongqiang Zhu, Jun Lu, Yongxiang Xia, Rong Wang","doi":"10.1016/j.celrep.2025.115350","DOIUrl":"10.1016/j.celrep.2025.115350","url":null,"abstract":"Metabolic dysfunction-associated steatotic liver disease (MASLD) is characterized by metabolic dysfunction and inflammation burden, involving a significant enhancement of cellular glycolytic activity. Here, we elucidate how a positive feedback loop in liver macrophages drives MASLD pathogenesis and demonstrate that disrupting this cycle mitigates metabolic stress and macrophage M1 activation during MASLD. We detect elevated expression of hexokinase 2 (HK2) and H3K18la in liver macrophages from patients with MASLD and MASLD mice. This lactate-dependent histone lactylation promotes glycolysis and liver macrophage M1 polarization by enriching the promoters of glycolytic genes and activating transcription. Ultimately, the HK2/glycolysis/H3K18la positive feedback loop exacerbates the vicious cycle of enhancing metabolic dysregulation and histone lactylation and the inflammatory phenotype of liver macrophages. Myeloid-specific deletion of Hk2 or pharmacological inhibition of the transcription factor HIF-1α significantly disrupts this deleterious cycle. Therefore, our study illustrates that targeting this amplified pathogenic loop may offer a promising therapeutic strategy for MASLD.","PeriodicalId":9798,"journal":{"name":"Cell reports","volume":"44 3","pages":"115350"},"PeriodicalIF":7.5,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143522678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The balance between IFN-γ and ERK/MAPK signaling activities ensures lifelong maintenance of intestinal stem cells.

IF 7.5 1区生物学

Cell reports Pub Date : 2025-03-25 Epub Date: 2025-02-13 DOI: 10.1016/j.celrep.2025.115286

May Nakajima-Koyama, Mio Kabata, Joonseong Lee, Yuko Sogabe, Satoko Sakurai, Akira Hirota, Mizuki Kimura, Tomonori Nakamura, Yusuke Imoto, Kohei Kometani, Yoko Hamazaki, Yasuaki Hiraoka, Mitinori Saitou, Eisuke Nishida, Takuya Yamamoto

{"title":"The balance between IFN-γ and ERK/MAPK signaling activities ensures lifelong maintenance of intestinal stem cells.","authors":"May Nakajima-Koyama, Mio Kabata, Joonseong Lee, Yuko Sogabe, Satoko Sakurai, Akira Hirota, Mizuki Kimura, Tomonori Nakamura, Yusuke Imoto, Kohei Kometani, Yoko Hamazaki, Yasuaki Hiraoka, Mitinori Saitou, Eisuke Nishida, Takuya Yamamoto","doi":"10.1016/j.celrep.2025.115286","DOIUrl":"10.1016/j.celrep.2025.115286","url":null,"abstract":"While the intestinal epithelium has the highest cellular turnover rates in the mammalian body, it is also considered one of the tissues most resilient to aging-related disorders. Here, we reveal an innate protective mechanism that safeguards intestinal stem cells (ISCs) from environmental conditions in the aged intestine. Using in vivo phenotypic analysis, transcriptomics, and in vitro intestinal organoid studies, we show that age-dependent activation of interferon-γ (IFN-γ) signaling and inactivation of extracellular signal-regulated kinase/mitogen-activated protein kinase (ERK/MAPK) signaling are responsible for establishing an equilibrium of Lgr5+ ISCs-between active and quiescent states-to preserve the ISC pool during aging. Furthermore, we show that differentiated cells have different sensitivities to each of the two signaling pathways, which may induce aging-related, functional, and metabolic changes in the body. Thus, our findings reveal an exquisitely balanced, age-dependent signaling mechanism that preserves stem cells at the expense of differentiated cells.","PeriodicalId":9798,"journal":{"name":"Cell reports","volume":" ","pages":"115286"},"PeriodicalIF":7.5,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143425014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

De-coding the complex role of microbial metabolites in cancer.

IF 7.5 1区生物学

Cell reports Pub Date : 2025-03-25 Epub Date: 2025-03-01 DOI: 10.1016/j.celrep.2025.115358

Pau Pérez Escriva, Catarina Correia Tavares Bernardino, Elisabeth Letellier

引用次数: 0

Functional inhibition of core spliceosomal machinery activates intronic premature cleavage and polyadenylation of pre-mRNAs.

IF 7.5 1区生物学

Cell reports Pub Date : 2025-03-25 Epub Date: 2025-02-27 DOI: 10.1016/j.celrep.2025.115376

Qiumin Feng, Zejin Lin, Danhui Zhao, Mengzhao Li, Sheng Yang, Andy Peng Xiang, Congting Ye, Chengguo Yao

引用次数: 0

Defining the molecular identity and morphology of glia limitans superficialis astrocytes in vertebrates.

IF 7.5 1区生物学

Cell reports Pub Date : 2025-03-25 Epub Date: 2025-02-20 DOI: 10.1016/j.celrep.2025.115344

Philip Hasel, Melissa L Cooper, Anne E Marchildon, Uriel Rufen-Blanchette, Rachel D Kim, Thong C Ma, Adam M R Groh, Emily J Hill, Eleanor M Lewis, Michał Januszewski, Sarah E W Light, Cody J Smith, Jo Anne Stratton, Steven A Sloan, Un Jung Kang, Moses V Chao, Shane A Liddelow

{"title":"Defining the molecular identity and morphology of glia limitans superficialis astrocytes in vertebrates.","authors":"Philip Hasel, Melissa L Cooper, Anne E Marchildon, Uriel Rufen-Blanchette, Rachel D Kim, Thong C Ma, Adam M R Groh, Emily J Hill, Eleanor M Lewis, Michał Januszewski, Sarah E W Light, Cody J Smith, Jo Anne Stratton, Steven A Sloan, Un Jung Kang, Moses V Chao, Shane A Liddelow","doi":"10.1016/j.celrep.2025.115344","DOIUrl":"10.1016/j.celrep.2025.115344","url":null,"abstract":"Astrocytes are a highly abundant glial cell type and perform critical homeostatic functions in the central nervous system. Like neurons, astrocytes have many discrete heterogeneous subtypes. The subtype identity and functions are, at least in part, associated with their anatomical location and can be highly restricted to strategically important anatomical domains. Here, we report that astrocytes forming the glia limitans superficialis, the outermost border of the brain and spinal cord, are a highly specialized astrocyte subtype and can be identified by a single marker: myocilin (Myoc). We show that glia limitans superficialis astrocytes cover the entire brain and spinal cord surface, exhibit an atypical morphology, and are evolutionarily conserved from zebrafish, rodents, and non-human primates to humans. Identification of this highly specialized astrocyte subtype will advance our understanding of CNS homeostasis and potentially be targeted for therapeutic intervention to combat peripheral inflammatory effects on the CNS.","PeriodicalId":9798,"journal":{"name":"Cell reports","volume":"44 3","pages":"115344"},"PeriodicalIF":7.5,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143472327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DA-DRD5 signaling reprograms B cells to promote CD8⁺ T cell-mediated antitumor immunity.

IF 7.5 1区生物学

Cell reports Pub Date : 2025-03-25 Epub Date: 2025-03-01 DOI: 10.1016/j.celrep.2025.115364

Yuqing Wu, Lei Zhu, Sheng Li, Lu Liu, Yaman Wang, Yongbing Yang, Yuan Mu, Qiuying Zhu, Yuying Jiang, Chunyan Wu, Peiwen Xi, Chunmei Ma, Lijun Liang, Min Gao, Yingchao Hu, Qiang Ding, Shiyang Pan

引用次数: 0

RNF10 and RIOK3 facilitate 40S ribosomal subunit degradation upon 60S biogenesis disruption or amino acid starvation.

IF 7.5 1区生物学

Cell reports Pub Date : 2025-03-25 Epub Date: 2025-02-28 DOI: 10.1016/j.celrep.2025.115371

Pierce W Ford, Danielle M Garshott, Mythreyi Narasimhan, Xuezhen Ge, Eric M Jordahl, Shubha Subramanya, Eric J Bennett

{"title":"RNF10 and RIOK3 facilitate 40S ribosomal subunit degradation upon 60S biogenesis disruption or amino acid starvation.","authors":"Pierce W Ford, Danielle M Garshott, Mythreyi Narasimhan, Xuezhen Ge, Eric M Jordahl, Shubha Subramanya, Eric J Bennett","doi":"10.1016/j.celrep.2025.115371","DOIUrl":"10.1016/j.celrep.2025.115371","url":null,"abstract":"The initiation-specific ribosome-associated quality control pathway (iRQC) is activated when translation initiation complexes fail to transition to elongation-competent 80S ribosomes. Upon iRQC activation, RNF10 ubiquitylates the 40S proteins uS3 and uS5, which leads to 40S decay. How iRQC is activated in the absence of pharmacological translation inhibitors and what mechanisms govern iRQC capacity and activity remain unanswered questions. Here, we demonstrate that altering 60S:40S stoichiometry by disrupting 60S biogenesis triggers iRQC activation and 40S decay. Depleting the critical scanning helicase eIF4A1 impairs 40S ubiquitylation and degradation, indicating mRNA engagement is required for iRQC. We show that amino acid starvation conditions also stimulate iRQC-dependent 40S decay. We identify RIOK3 as a crucial iRQC factor that interacts with ubiquitylated 40S subunits to mediate degradation. Both RNF10 and RIOK3 protein levels increase upon iRQC pathway activation, establishing a feedforward mechanism that regulates iRQC capacity and subsequent 40S decay.","PeriodicalId":9798,"journal":{"name":"Cell reports","volume":"44 3","pages":"115371"},"PeriodicalIF":7.5,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143536758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An IL-27-Driven Transcriptional Network Identifies Regulators of IL-10 Expression across T Helper Cell Subsets.

IF 7.5 1区生物学

Cell reports Pub Date : 2025-03-25 Epub Date: 2025-03-08 DOI: 10.1016/j.celrep.2025.115455

Huiyuan Zhang, Asaf Madi, Nir Yosef, Norio Chihara, Amit Awasthi, Caroline Pot, Conner Lambden, Amitabh Srivastava, Patrick R Burkett, Jackson Nyman, Elena Christian, Yasaman Etminan, Annika Lee, Helene Stroh, Junrong Xia, Katarzyna Karwacz, Pratiksha I Thakore, Nandini Acharya, Alexandra Schnell, Chao Wang, Lionel Apetoh, Orit Rozenblatt-Rosen, Ana C Anderson, Aviv Regev, Vijay K Kuchroo

引用次数: 0