Cell reports最新文献

Time-resolved single-cell atlas identifies the spatiotemporal transcription dynamics in vernalization response in Brassica rapa. 时间分辨单细胞图谱鉴定了油菜春化反应的时空转录动力学。

IF 7.5 1区生物学

Cell reports Pub Date : 2025-05-15 DOI: 10.1016/j.celrep.2025.115725

Zhicheng Zhang, Xu Cai, Jianli Liang, Jiahe Liu, Jing Guo, Wencai Yang, Xiaowu Wang, Jian Wu

{"title":"Time-resolved single-cell atlas identifies the spatiotemporal transcription dynamics in vernalization response in Brassica rapa.","authors":"Zhicheng Zhang, Xu Cai, Jianli Liang, Jiahe Liu, Jing Guo, Wencai Yang, Xiaowu Wang, Jian Wu","doi":"10.1016/j.celrep.2025.115725","DOIUrl":"https://doi.org/10.1016/j.celrep.2025.115725","url":null,"abstract":"Many temperate plants require vernalization, a prolonged low-temperature period, to accelerate flowering. Vernalization is a quantitative process whereby extended cold exposure establishes a stable transcriptional repression, with the degree of silencing correlating with the length of cold treatment. While much is known about the genes regulating this process, the expression dynamics at the single-cell level remain elusive. Using single-cell RNA sequencing, we analyze the vernalization response in Brassica rapa. Our data show that mesophyll cells exhibit the most significant changes in gene expression at low temperatures, whereas vasculature exhibits higher expression levels of flowering-related genes. Mesophyll trajectory analyses suggest that B. rapa plants undergo a biphasic response to chill stress during vernalization. Tissue-wide BrFLC expression changes result from variations in the proportion of expressing cells, supporting the quantitative nature of vernalization through digital cell responses. This study provides valuable resources and insights into the spatiotemporal regulation of flowering during vernalization.","PeriodicalId":9798,"journal":{"name":"Cell reports","volume":"44 5","pages":"115725"},"PeriodicalIF":7.5,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144076285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vaccination against helminth IL-33 modulators permits immune-mediated parasite ejection. 针对蠕虫IL-33调节剂的疫苗接种允许免疫介导的寄生虫喷射。

IF 7.5 1区生物学

Cell reports Pub Date : 2025-05-15 DOI: 10.1016/j.celrep.2025.115721

Danielle J Smyth, Suzanne H Hodge, Nicole W P Ong, Josh Richards, Florent Colomb, Samuele Di Carmine, Vivien Shek, Tania Frangova, Marta Campillo Poveda, Rick M Maizels, Henry J McSorley

{"title":"Vaccination against helminth IL-33 modulators permits immune-mediated parasite ejection.","authors":"Danielle J Smyth, Suzanne H Hodge, Nicole W P Ong, Josh Richards, Florent Colomb, Samuele Di Carmine, Vivien Shek, Tania Frangova, Marta Campillo Poveda, Rick M Maizels, Henry J McSorley","doi":"10.1016/j.celrep.2025.115721","DOIUrl":"https://doi.org/10.1016/j.celrep.2025.115721","url":null,"abstract":"The murine intestinal nematode Heligmosomoides polygyrus bakeri (Hpb) modulates the host immune response via the Hpb alarmin release inhibitor (HpARI) family (HpARI1/2/3), which acts on interleukin (IL)-33, and the Hpb binds alarmin receptor and inhibits (HpBARI) family (HpBARI and HpBARI_Hom2), which acts on the IL-33 receptor ST2. Here, we find that this immunomodulation is evident only in the first week of infection and affects local and distal tissues. Vaccination with HpARI or HpBARI proteins raises antibody responses that block their immunomodulatory activities: HpARI2 vaccination results in significantly increased type 2 innate lymphoid cells (ILC2s), T helper (Th)2, and serum IL-4 and IL-5 responses, while HpBARI + HpBARI_Hom2 vaccination reverses infection-mediated ST2 suppression and increases Th2 immunity. A cocktail of HpARI2 + HpBARI + HpBARI_Hom2 gives robust protection against infection, associated with stunting of adult parasites, reduced egg burden, increased type 2 immune responses, and intestinal goblet cell expansion. Therefore, vaccination with immunomodulatory proteins can protect the host against infection and can be used as a tool for blocking the effects of specific parasite-derived proteins.","PeriodicalId":9798,"journal":{"name":"Cell reports","volume":"44 5","pages":"115721"},"PeriodicalIF":7.5,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144076288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mapping essential somatic hypermutations in a CD4-binding site bNAb informs HIV-1 vaccine design. 绘制cd4结合位点bNAb的基本体细胞超突变为HIV-1疫苗设计提供信息。

IF 7.5 1区生物学

Cell reports Pub Date : 2025-05-15 DOI: 10.1016/j.celrep.2025.115713

Kim-Marie A Dam, Harry B Gristick, Yancheng E Li, Zhi Yang, Priyanthi N P Gnanapragasam, Anthony P West, Michael S Seaman, Pamela J Bjorkman

{"title":"Mapping essential somatic hypermutations in a CD4-binding site bNAb informs HIV-1 vaccine design.","authors":"Kim-Marie A Dam, Harry B Gristick, Yancheng E Li, Zhi Yang, Priyanthi N P Gnanapragasam, Anthony P West, Michael S Seaman, Pamela J Bjorkman","doi":"10.1016/j.celrep.2025.115713","DOIUrl":"https://doi.org/10.1016/j.celrep.2025.115713","url":null,"abstract":"HIV-1 broadly neutralizing antibodies (bNAbs) targeting the CD4-binding site (CD4bs) contain rare features that pose challenges to elicit these bNAbs through vaccination. The IOMA class of CD4bs bNAbs includes fewer rare features and somatic hypermutations (SHMs) to achieve broad neutralization, thus presenting a potentially accessible pathway for vaccine-induced bNAb development. Here, we created a library of IOMA variants in which each SHM was individually reverted to the inferred germline counterpart to investigate the roles of SHMs in conferring IOMA's neutralization potency and breadth. Impacts on neutralization for each variant were evaluated, and this information was used to design minimally mutated IOMA-class variants (IOMAmin) that incorporated the fewest SHMs required for achieving IOMA's neutralization breadth. A cryoelectron microscopy (cryo-EM) structure of an IOMAmin variant bound to Env was used to further interpret characteristics of IOMA variants to elucidate how IOMA's structural features correlate with its neutralization mechanism, informing the design of IOMA-targeting immunogens.","PeriodicalId":9798,"journal":{"name":"Cell reports","volume":"44 5","pages":"115713"},"PeriodicalIF":7.5,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144076277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

OsHYPK/NatA-mediated N-terminal acetylation regulates the homeostasis of NLR immune protein to fine-tune rice immune responses and growth. OsHYPK/ nata介导的n端乙酰化调节NLR免疫蛋白的稳态，调控水稻的免疫应答和生长。

IF 7.5 1区生物学

Cell reports Pub Date : 2025-05-15 DOI: 10.1016/j.celrep.2025.115719

Yaqian Huang, Xiaodi Gong, Hui Shi, Peiyi Wang, Yundong Yuan, Cuilian Kong, Jie Zhou, Dianxing Wu, Yan Liang, Yonghong Wang, Jing Wang

{"title":"OsHYPK/NatA-mediated N-terminal acetylation regulates the homeostasis of NLR immune protein to fine-tune rice immune responses and growth.","authors":"Yaqian Huang, Xiaodi Gong, Hui Shi, Peiyi Wang, Yundong Yuan, Cuilian Kong, Jie Zhou, Dianxing Wu, Yan Liang, Yonghong Wang, Jing Wang","doi":"10.1016/j.celrep.2025.115719","DOIUrl":"https://doi.org/10.1016/j.celrep.2025.115719","url":null,"abstract":"Keeping nucleotide-binding leucine-rich repeat (NLR) protein at appropriate levels is critical for plant survival. Huntingtin Yeast Partner K (OsHYPK) was previously identified as a positive regulator of N-terminal acetyltransferase A (NatA) activity in rice. Here, we find that oshypk shows enhanced resistance to Magnaporthe oryzae (M. oryzae). Through screening for suppressors of oshypk (soh), we identify suppressor soh74, which contains a mutation in RESISTANCE TO P. SYRINGAE PV MACULICOLA1 (RPM1)-like NLR protein (RPM1-L1) and exhibits compromised resistance to M. oryzae. Mechanistically, declined N-terminal acetylation (NTA) degree in oshypk leads to protein accumulation of RPM1-L1, contributing to enhanced disease resistance. To restrict RPM1-L1 accumulation, OsHYPK is expressed at high levels under normal conditions. However, pathogen infection reduces OsHYPK level to release the inhibition on RPM1-L1, leading to immune activation. This study reveals a vital pathway in which OsHYPK/NatA-mediated NTA rapidly fine-tunes NLR-mediated immune response.","PeriodicalId":9798,"journal":{"name":"Cell reports","volume":"44 5","pages":"115719"},"PeriodicalIF":7.5,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144076278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cas12h is a crRNA-guided DNA nickase that can be utilized for precise gene editing. Cas12h是一种crrna引导的DNA缺口酶，可用于精确的基因编辑。

IF 7.5 1区生物学

Cell reports Pub Date : 2025-05-14 DOI: 10.1016/j.celrep.2025.115718

Wenwen Xiang, Xiaofeng Lin, Yunqian Yang, Linglong Huang, Ying Chen, Jiyun Chen, Liang Liu

引用次数: 0

Adipocyte metabolic state regulates glial phagocytic function. 脂肪细胞代谢状态调节胶质细胞吞噬功能。

IF 7.5 1区生物学

Cell reports Pub Date : 2025-05-14 DOI: 10.1016/j.celrep.2025.115704

Mroj Alassaf, Aditi Madan, Sunidhi Ranganathan, Shannon Marschall, Jordan J Wong, Zachary H Goldberg, Ava E Brent, Akhila Rajan

{"title":"Adipocyte metabolic state regulates glial phagocytic function.","authors":"Mroj Alassaf, Aditi Madan, Sunidhi Ranganathan, Shannon Marschall, Jordan J Wong, Zachary H Goldberg, Ava E Brent, Akhila Rajan","doi":"10.1016/j.celrep.2025.115704","DOIUrl":"https://doi.org/10.1016/j.celrep.2025.115704","url":null,"abstract":"Excess dietary sugar profoundly impacts organismal metabolism and health, yet it remains unclear how metabolic adaptations in adipose tissue influence other organs, including the brain. Here, we show that a high-sugar diet (HSD) in Drosophila reduces adipocyte glycolysis and mitochondrial pyruvate uptake, shifting metabolism toward fatty acid oxidation and ketogenesis. These metabolic changes trigger mitochondrial oxidation and elevate antioxidant responses. Adipocyte-specific manipulations of glycolysis, lipid metabolism, or mitochondrial dynamics non-autonomously modulate Draper expression in brain ensheathing glia, key cells responsible for neuronal debris clearance. Adipocyte-derived ApoB-containing lipoproteins maintain basal Draper levels in glia via LpR1, critical for effective glial phagocytic activity. Accordingly, reducing ApoB or LpR1 impairs glial clearance of degenerating neuronal debris after injury. Collectively, our findings demonstrate that dietary sugar-induced shifts in adipocyte metabolism substantially influence brain health by modulating glial phagocytosis, identifying adipocyte-derived ApoB lipoproteins as essential systemic mediators linking metabolic state with neuroprotective functions.","PeriodicalId":9798,"journal":{"name":"Cell reports","volume":"44 5","pages":"115704"},"PeriodicalIF":7.5,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144076145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The impact of breast radiotherapy on the tumor genome and immune ecosystem. 乳腺放疗对肿瘤基因组和免疫生态系统的影响。

IF 7.5 1区生物学

Cell reports Pub Date : 2025-05-14 DOI: 10.1016/j.celrep.2025.115703

Aislyn Schalck, Tuan Tran, Jianzhuo Li, Emi Sei, Shanshan Bai, Min Hu, Jerome Lin, Scott J Bright, Samuel Reddick, Fei Yang, Harsh Batra, Alejandro Contreras, Maria Gabriela Raso, Michael C Stauder, Karen E Hoffman, Jay P Reddy, Kevin T Nead, Benjamin D Smith, Gabriel O Sawakuchi, Wendy A Woodward, Stephanie S Watowich, Jennifer K Litton, Isabelle Bedrosian, Elizabeth A Mittendorf, Huong Le-Petross, Nicholas E Navin, Simona F Shaitelman

{"title":"The impact of breast radiotherapy on the tumor genome and immune ecosystem.","authors":"Aislyn Schalck, Tuan Tran, Jianzhuo Li, Emi Sei, Shanshan Bai, Min Hu, Jerome Lin, Scott J Bright, Samuel Reddick, Fei Yang, Harsh Batra, Alejandro Contreras, Maria Gabriela Raso, Michael C Stauder, Karen E Hoffman, Jay P Reddy, Kevin T Nead, Benjamin D Smith, Gabriel O Sawakuchi, Wendy A Woodward, Stephanie S Watowich, Jennifer K Litton, Isabelle Bedrosian, Elizabeth A Mittendorf, Huong Le-Petross, Nicholas E Navin, Simona F Shaitelman","doi":"10.1016/j.celrep.2025.115703","DOIUrl":"https://doi.org/10.1016/j.celrep.2025.115703","url":null,"abstract":"Radiotherapy is a pillar of breast cancer treatment; however, it remains unclear how radiotherapy modulates the tumor microenvironment. We investigated this question in a cohort of 20 patients with estrogen-receptor positive (ER+) breast tumors who received neoadjuvant radiotherapy. Tumor biopsies were collected before and 7 days postradiation. Single-cell DNA sequencing (scDNA-seq) and scRNA-seq were conducted on 8 and 11 patients, respectively, at these two time points. The scRNA data showed increased infiltration of naive-like CD4 T cells and an early, activated CD8 T cell population following radiotherapy. Radiotherapy also eliminated existing cytotoxic T cells and resulted in myeloid cell increases. In tumor cells, the scDNA-seq data showed a high genomic selection of subclones in half of the patients with high ER expression, while the remaining number had low genomic selection and an interferon response. Collectively, these data provide insight into the impact of radiotherapy in ER+ breast cancer patients.","PeriodicalId":9798,"journal":{"name":"Cell reports","volume":"44 5","pages":"115703"},"PeriodicalIF":7.5,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144076282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HSPA9 contributes to tumor progression and ferroptosis resistance by enhancing USP14-driven SLC7A11 deubiquitination in multiple myeloma. HSPA9通过增强多发性骨髓瘤中usp14驱动的SLC7A11去泛素化，促进肿瘤进展和铁凋亡抵抗。

IF 7.5 1区生物学

Cell reports Pub Date : 2025-05-14 DOI: 10.1016/j.celrep.2025.115720

Na Shen, Yuan Xia, Xuxing Shen, Wei Hua, Min Shi, Lijuan Chen

{"title":"HSPA9 contributes to tumor progression and ferroptosis resistance by enhancing USP14-driven SLC7A11 deubiquitination in multiple myeloma.","authors":"Na Shen, Yuan Xia, Xuxing Shen, Wei Hua, Min Shi, Lijuan Chen","doi":"10.1016/j.celrep.2025.115720","DOIUrl":"https://doi.org/10.1016/j.celrep.2025.115720","url":null,"abstract":"Ferroptosis, a regulated cell death triggered by overload-dependent lipid peroxidation, is implicated in multiple human cancers. The mechanisms underlying ferroptosis in multiple myeloma (MM) remain enigmatic. Here, we confirmed that HSPA9 is overexpressed in MM samples and correlates with unfavorable outcomes. Functionally, HSPA9 enhances MM cell viability, ferroptosis resistance, and tumorigenicity, suggesting its oncogenic role. Proteomics screening identified SLC7A11, a key ferroptosis suppressor, as a HSPA9 interactor. Mechanistically, HSPA9 serves as a bridge to strengthen the interaction between USP14 and SLC7A11, modulating USP14-mediated SLC7A11 deubiquitination. Furthermore, the inhibition of USP14 with IU1 enhances the SLC7A11 ubiquitination and degradation, promoting ferroptosis and showing therapeutic efficacy in MM xenograft models. Clinically, HSPA9, USP14, and SLC7A11 expression are positively correlated in MM samples, which have a prognostic value. Our study reveals HSPA9-USP14-SLC7A11 axis as a key regulator of ferroptosis in MM and a potential therapeutic target.","PeriodicalId":9798,"journal":{"name":"Cell reports","volume":"44 5","pages":"115720"},"PeriodicalIF":7.5,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144076273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Septo-subicular cholinergic circuit promotes seizure development via astrocytic inflammation. 间隔-丘下胆碱能回路通过星形细胞炎症促进癫痫发作的发展。

IF 7.5 1区生物学

Cell reports Pub Date : 2025-05-14 DOI: 10.1016/j.celrep.2025.115712

Yu Wang, Qingyang Zhang, Fan Fei, Keyu Hu, Fei Wang, Heming Cheng, Cenglin Xu, Lingyu Xu, Jiannong Wu, Vladimir Parpura, Zhong Chen, Yi Wang

{"title":"Septo-subicular cholinergic circuit promotes seizure development via astrocytic inflammation.","authors":"Yu Wang, Qingyang Zhang, Fan Fei, Keyu Hu, Fei Wang, Heming Cheng, Cenglin Xu, Lingyu Xu, Jiannong Wu, Vladimir Parpura, Zhong Chen, Yi Wang","doi":"10.1016/j.celrep.2025.115712","DOIUrl":"https://doi.org/10.1016/j.celrep.2025.115712","url":null,"abstract":"The central dogma explaining epileptic seizures largely revolves around the classic theory of \"excitability-inhibition\" imbalance between glutamatergic and GABAergic transmission. Cholinergic neurons play a significant role in epilepsy; however, these neuronal populations are molecularly and structurally heterogeneous. Here, we show a subpopulation of subiculum-projecting septal cholinergic neurons that promote seizure development. Functionally, this subpopulation is suppressed during seizures. Selective manipulation of the septo-subicular cholinergic circuit bidirectionally regulates the development of hippocampal seizures. Notably, cholinergic signaling enhances subicular astrocytic caspase-1-mediated neuroinflammation via M3 muscarinic receptors, increasing excitatory synaptic transmission and promoting seizure development. Together, these results demonstrate that activation of the septo-subicular cholinergic circuits facilitates seizure development via astrocytic inflammation. Our findings provide insight into the cholinergic mechanism involved in epilepsy and suggest targeted therapeutic strategies for epilepsy treatment, focusing on the specific cholinergic neuronal subpopulation.","PeriodicalId":9798,"journal":{"name":"Cell reports","volume":"44 5","pages":"115712"},"PeriodicalIF":7.5,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144076280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bisphenol S induces brown adipose tissue whitening and aggravates diet-induced obesity in an estrogen-dependent manner. 双酚S诱导棕色脂肪组织变白，并以雌激素依赖的方式加重饮食引起的肥胖。

IF 7.5 1区生物学

Cell reports Pub Date : 2025-05-14 DOI: 10.1016/j.celrep.2025.115744

Xue Wen, Yang Xiao, Haitao Xiao, Xueqin Tan, Beiyi Wu, Zehua Li, Ru Wang, Xuewen Xu, Tao Li

引用次数: 0