Cell reports最新文献_第2页

Sound preferences in mice are sex dependent. 老鼠的声音偏好与性别有关。

IF 6.9 1区生物学

Cell reports Pub Date : 2025-10-17 DOI: 10.1016/j.celrep.2025.116454

Kamini Sehrawat, Israel Nelken

引用次数: 0

Med20 regulates myelination in the peripheral nervous system by modulating ferroptosis of Schwann cells. Med20通过调节雪旺细胞的铁凋亡来调节周围神经系统的髓鞘形成。

IF 6.9 1区生物学

Cell reports Pub Date : 2025-10-16 DOI: 10.1016/j.celrep.2025.116448

Wenyi Yang, Xiang Chen, Chunyan Yang, Yanqing Mao, Wei Zhuang, Yunshan Xiao, Xueqin Zhang, Zhimin Ou, Ying Chen

{"title":"Med20 regulates myelination in the peripheral nervous system by modulating ferroptosis of Schwann cells.","authors":"Wenyi Yang, Xiang Chen, Chunyan Yang, Yanqing Mao, Wei Zhuang, Yunshan Xiao, Xueqin Zhang, Zhimin Ou, Ying Chen","doi":"10.1016/j.celrep.2025.116448","DOIUrl":"https://doi.org/10.1016/j.celrep.2025.116448","url":null,"abstract":"Myelin sheath is essential for the maintenance of neural system functions. The mediator complex (MED) is associated with neurodegenerative diseases. Mutation of the MED subunit Med20 leads to abnormal brain development, but whether Med20 modulates myelination remains unknown. In this study, we report that Med20 is an important modulator of myelination in the peripheral nervous system. Loss of Med20 in Schwann cells (SCs) induces ferroptosis, thus impairing myelination. The functions of Med20 in SCs are mediated by its downstream target DDB1. DDB1 controls SC ferroptosis by regulating Hmox1 through a \"DDB1-UHRF1-BACH1-Hmox1\" transcription regulation axis and its direct interaction and subsequent simultaneous ubiquitination of the HO-1 protein. HO-1 inhibitor ZnPP or ferroptosis inhibitor Fer-1 is sufficient and efficient for antagonizing ferroptosis in SCs and restoring myelination in Med20-deficient mice. The results of our study reveal that Med20 is an important regulator in the network that controls myelination in the peripheral nervous system.","PeriodicalId":9798,"journal":{"name":"Cell reports","volume":"44 10","pages":"116448"},"PeriodicalIF":6.9,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145318180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing pre-sexual and sexual differentiation of Toxoplasma gondii using retinal epithelial cells and intestinal organoids. 利用视网膜上皮细胞和肠道类器官促进刚地弓形虫的性前分化和性分化。

IF 6.9 1区生物学

Cell reports Pub Date : 2025-10-16 DOI: 10.1016/j.celrep.2025.116451

Saira Cancela, Florencia Sena, Romina Pagotto, Martina Crispo, Maria E Francia, Mariela Bollati-Fogolín

{"title":"Enhancing pre-sexual and sexual differentiation of Toxoplasma gondii using retinal epithelial cells and intestinal organoids.","authors":"Saira Cancela, Florencia Sena, Romina Pagotto, Martina Crispo, Maria E Francia, Mariela Bollati-Fogolín","doi":"10.1016/j.celrep.2025.116451","DOIUrl":"https://doi.org/10.1016/j.celrep.2025.116451","url":null,"abstract":"Toxoplasmosis, caused by Toxoplasma gondii, is a major global health concern due to its high prevalence, zoonotic transmission, and economic impact on livestock. The parasite's life cycle includes asexual, pre-sexual, and sexual stages, the latter responsible for oocyst shedding and genetic recombination. Understanding sexual differentiation is critical, but access has been limited because these stages occur only in the feline intestinal epithelium. Recent studies identify host metabolic cues and the microrchidia (MORC) protein complex as regulators of sexual commitment. We optimize in vitro approaches to enrich pre-sexual and sexual stages by combining a human retinal epithelial cell line and murine intestinal organoids with FELIX medium, which mimics feline intestinal biochemistry, and conditional MORC depletion. This system increases stage-specific gene expression and marker detection, demonstrating synergistic effects of host environment and genetic regulation. Our findings provide accessible models to study T. gondii sexual differentiation, with implications for controlling transmission and genetic diversity.","PeriodicalId":9798,"journal":{"name":"Cell reports","volume":"44 10","pages":"116451"},"PeriodicalIF":6.9,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145318212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heat shock induces silent ribosomes and reorganizes mRNA turnover. 热休克诱导沉默核糖体并重组mRNA周转。

IF 6.9 1区生物学

Cell reports Pub Date : 2025-10-16 DOI: 10.1016/j.celrep.2025.116447

Sayanur Rahaman, Nicole Schiffelholz, Nitish Mittal, Klemens E Fröhlich, Mihaela Zavolan, Attila Becskei

{"title":"Heat shock induces silent ribosomes and reorganizes mRNA turnover.","authors":"Sayanur Rahaman, Nicole Schiffelholz, Nitish Mittal, Klemens E Fröhlich, Mihaela Zavolan, Attila Becskei","doi":"10.1016/j.celrep.2025.116447","DOIUrl":"https://doi.org/10.1016/j.celrep.2025.116447","url":null,"abstract":"mRNAs associate with single or multiple ribosomes; these ribosomal assemblies-monosomes and polysomes-translate the mRNAs before degradation. The impact of heat stress on this mRNA turnover remains unclear. We show that in heat-shocked yeast cells, the proportion of monosomes increases without a corresponding rise in the number of associated mRNAs. Consequently, most monosomes are devoid of mRNAs and silent, lacking translational initiation factors and proteins facilitating posttranslational folding. Such silent monosomes also appear under other stress conditions, with proportions varying according to stress type, suggesting that they represent a general feature of cellular adaptation. In parallel with the induction of silent ribosomes, elevated temperatures reduce the overall rate of mRNA-ribosome association with few exceptions. Notably, heat shock promotes the ribosomal association of transcripts encoding heat shock proteins, without extension of the half-lives of these mRNAs. These mechanisms dynamically reorganize mRNA turnover to prioritize the translation of heat shock proteins over other proteins.","PeriodicalId":9798,"journal":{"name":"Cell reports","volume":"44 10","pages":"116447"},"PeriodicalIF":6.9,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145318154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modulation of speed-dependent acetylcholine release in the hippocampus by spatial task engagement. 空间任务参与对海马速度依赖性乙酰胆碱释放的调节。

IF 6.9 1区生物学

Cell reports Pub Date : 2025-10-16 DOI: 10.1016/j.celrep.2025.116443

Feng Xuan, Guochuan Li, Yulong Li, Daniel A Dombeck

引用次数: 0

Regulation of epigenetics and chromosome structure by human ORC2. 人类ORC2对表观遗传学和染色体结构的调控。

IF 6.9 1区生物学

Cell reports Pub Date : 2025-10-16 DOI: 10.1016/j.celrep.2025.116504

Zhangli Su, Mengxue Tian, Etsuko Shibata, Yoshiyuki Shibata, Tianyi Yang, Zhenjia Wang, Fulai Jin, Chongzhi Zang, Anindya Dutta

引用次数: 0

Multi-environment deep mutational scanning reveals the distribution of temperature-sensitive variants in a bacterial kinase. 多环境深度突变扫描揭示了细菌激酶中温度敏感变异的分布。

IF 6.9 1区生物学

Cell reports Pub Date : 2025-10-16 DOI: 10.1016/j.celrep.2025.116446

Dia A Ghose, Carl B W Soderstrom, Emily M Mahoney, Michael T Laub

{"title":"Multi-environment deep mutational scanning reveals the distribution of temperature-sensitive variants in a bacterial kinase.","authors":"Dia A Ghose, Carl B W Soderstrom, Emily M Mahoney, Michael T Laub","doi":"10.1016/j.celrep.2025.116446","DOIUrl":"https://doi.org/10.1016/j.celrep.2025.116446","url":null,"abstract":"Deep mutational scanning is a powerful technique for determining sequence-function landscapes of biological macromolecules, but it is often performed under a single condition. Thus, how changing environmental conditions affect these landscapes remains opaque. Here, we address this by performing multi-environment deep mutational scanning to characterize the functional landscape of a bacterial kinase at multiple temperatures. By doing so, we systematically identify temperature-sensitive (ts) and temperature-resistant variants, providing a global view of their prevalence and identities. Substitutions leading to temperature-associated changes in activity are rare, reflecting high mutational tolerance across conditions, but ts and temperature-resistant substitutions are identified. In contrast to existing paradigms, we find that substitutions causing temperature sensitivity are prevalent in both the protein core and the surface. Temperature-resistant variants also arise but exhibit increased enzymatic activity, not improved thermal stability. Our results could not be recapitulated by the state-of-the-art computational stability prediction, demonstrating the importance of systematic experimental approaches to identifying condition-dependent mutation effects.","PeriodicalId":9798,"journal":{"name":"Cell reports","volume":"44 10","pages":"116446"},"PeriodicalIF":6.9,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145312528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

STBD1 mediates the crosstalk between glycogen and lipid droplets in clear cell renal cell carcinoma. STBD1介导透明细胞肾细胞癌中糖原和脂滴间的串扰。

IF 6.9 1区生物学

Cell reports Pub Date : 2025-10-15 DOI: 10.1016/j.celrep.2025.116429

Hao Wang, Tong Xiao, Hao Zhuang, Yi Liu, Kexing Jin, Jing Li, Jun Li, Yan Gao, Dan Yue, Wei Cui, Qingxia Xu, Jingxuan Pan, Kangdong Liu, Yong Wang, Ruibing Chen, Hongle Li

{"title":"STBD1 mediates the crosstalk between glycogen and lipid droplets in clear cell renal cell carcinoma.","authors":"Hao Wang, Tong Xiao, Hao Zhuang, Yi Liu, Kexing Jin, Jing Li, Jun Li, Yan Gao, Dan Yue, Wei Cui, Qingxia Xu, Jingxuan Pan, Kangdong Liu, Yong Wang, Ruibing Chen, Hongle Li","doi":"10.1016/j.celrep.2025.116429","DOIUrl":"https://doi.org/10.1016/j.celrep.2025.116429","url":null,"abstract":"The accumulation of lipid droplets (LDs) and glycogen is a major hallmark of clear cell renal cell carcinoma (ccRCC), yet their interplay remains unclear. By proteomic profiling of 50 ccRCC tumors, we observe activation of glycogen- and LD-related pathways. Using proximity labeling of the LD proteome, we identify starch-binding domain-containing protein 1 (STBD1), a glycogen-binding protein involved in glycophagy, as a novel LD component. Further mechanistic investigation shows that STBD1 targets LDs via N-terminal myristoylation and mediates glycogen-LD colocalization. Its depletion decreases LD abundance and impairs both glycophagy and lipophagy, suggesting a critical role of STBD1 in both the biogenesis and autophagic degradation of LDs. Furthermore, STBD1 knockdown alters lipid composition, enhances ferroptosis sensitivity, and suppresses tumor growth both in vitro and in vivo. Collectively, our findings establish STBD1 as a critical mediator of glycogen-LD crosstalk and highlight its potential as a therapeutic target in ccRCC.","PeriodicalId":9798,"journal":{"name":"Cell reports","volume":"44 10","pages":"116429"},"PeriodicalIF":6.9,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145312587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adipose m⁶A reader YTHDF2 promotes obesity, insulin resistance, and liver steatosis by suppressing β-adrenergic signaling and lipolysis. 脂肪m6A读取器YTHDF2通过抑制β-肾上腺素能信号和脂肪分解促进肥胖、胰岛素抵抗和肝脏脂肪变性。

IF 6.9 1区生物学

Cell reports Pub Date : 2025-10-15 DOI: 10.1016/j.celrep.2025.116445

Ruoyu Zhou, Liping Ju, Qianqian Kang, Qiantao Zheng, Decheng Ren, Liangyou Rui

{"title":"Adipose m6A reader YTHDF2 promotes obesity, insulin resistance, and liver steatosis by suppressing β-adrenergic signaling and lipolysis.","authors":"Ruoyu Zhou, Liping Ju, Qianqian Kang, Qiantao Zheng, Decheng Ren, Liangyou Rui","doi":"10.1016/j.celrep.2025.116445","DOIUrl":"https://doi.org/10.1016/j.celrep.2025.116445","url":null,"abstract":"RNA N6-methyladenosine (m6A) modification induces catecholamine resistance and lipolysis inhibition in white adipose tissue (WAT), contributing to obesity pathogenesis; however, the responsible m6A readers remain elusive. Here, we identify YTHDF2 as a key m6A reader governing both β-adrenergic signaling and lipolytic machinery. YTHDF2 binds to m6A-marked mRNAs encoding β3-adrenergic receptor (Adrb3), adipose triacylglycerol lipase (Atgl), and comparative gene identification-58 (Cgi-58), promoting their degradation and thereby suppressing β-adrenergic signaling and lipolysis. Deletion of adipose Ythdf2 enhances lipolysis in vivo, in WAT explants ex vivo, and in cultured adipocytes. Conversely, YTHDF2 overexpression suppresses adipocyte lipolysis. High-fat diet feeding upregulates adipose YTHDF2 and increases its binding to Adrb3, Atgl, and Cgi-58 mRNAs. Adipocyte-specific deletion of Ythdf2 protects against diet-induced obesity, insulin resistance, and liver steatosis. Moreover, deletion of adipose Mettl14-but not Ythdf2-disrupts brown adipose tissue development. These results unveil an adipose-intrinsic METTL3/METTL14/m6A/YTHDF2 pathway that drives catecholamine resistance and lipolysis suppression in obesity.","PeriodicalId":9798,"journal":{"name":"Cell reports","volume":"44 10","pages":"116445"},"PeriodicalIF":6.9,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145306898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The m⁶A reader IGF2BP2 promotes homologous recombination-mediated DNA repair by eliminating DNA-RNA hybrids. m6A读取器IGF2BP2通过消除DNA- rna杂交促进同源重组介导的DNA修复。

IF 6.9 1区生物学

Cell reports Pub Date : 2025-10-15 DOI: 10.1016/j.celrep.2025.116438

Yating Sun, Haojie Zhang, Shiwei Li, Jinzhi Zhang, Bin Lyu, Liping Kang, Xinkun Teng, Mingwei Yin, Weidong Peng, Jingjing Wang, Zhimin Chu, Chengying Cui, Dejie Kong, Mingqing Wu, Yongqiang Wang, Jiadong Wang, Yang Li

引用次数: 0