IF 8.3 2区生物学

Cells Pub Date : 2024-10-23 DOI: 10.3390/cells13211748

Hans Knecht, Tina Petrogiannis-Haliotis, Sherif Louis, Sabine Mai

{"title":"3D-Q-FISH/Telomere/TRF2 Nanotechnology Identifies a Progressively Disturbed Telomere/Shelterin/Lamin AC Complex as the Common Pathogenic, Molecular/Spatial Denominator of Classical Hodgkin Lymphoma.","authors":"Hans Knecht, Tina Petrogiannis-Haliotis, Sherif Louis, Sabine Mai","doi":"10.3390/cells13211748","DOIUrl":"10.3390/cells13211748","url":null,"abstract":"The bi- or multinucleated Reed-Sternberg cell (RS) is the diagnostic cornerstone of Epstein-Barr Virus (EBV)-positive and EBV-negative classical Hodgkin lymphoma (cHL). cHL is a germinal center (GC)-derived B-cell disease. Hodgkin cells (H) are the mononuclear precursors of RS. An experimental model has to fulfill three conditions to qualify as common pathogenic denominator: (i) to be of GC-derived B-cell origin, (ii) to be EBV-negative to avoid EBV latency III expression and (iii) to support permanent EBV-encoded oncogenic latent membrane protein (LMP1) expression upon induction. These conditions are unified in the EBV-, diffuse large B-Cell lymphoma (DLBCL) cell line BJAB-tTA-LMP1. 3D reconstructive nanotechnology revealed spatial, quantitative and qualitative disturbance of telomere/shelterin interactions in mononuclear H-like cells, with further progression during transition to RS-like cells, including progressive complexity of the karyotype with every mitotic cycle, due to BBF (breakage/bridge/fusion) events. The findings of this model were confirmed in diagnostic patient samples and correlate with clinical outcomes. Moreover, in vitro, significant disturbance of the lamin AC/telomere interaction progressively occurred. In summary, our research over the past three decades identified cHL as the first lymphoid malignancy driven by a disturbed telomere/shelterin/lamin AC interaction, generating the diagnostic RS. Our findings may act as trailblazer for tailored therapies in refractory cHL.","PeriodicalId":9743,"journal":{"name":"Cells","volume":"13 21","pages":""},"PeriodicalIF":8.3,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11545283/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142602995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Atomic Force Microscopy and Scanning Ion-Conductance Microscopy for Investigation of Biomechanical Characteristics of Neutrophils. 原子力显微镜和扫描离子电导显微镜用于研究中性粒细胞的生物力学特征

IF 8.3 2区生物学

Cells Pub Date : 2024-10-23 DOI: 10.3390/cells13211757

Mikhail Shvedov, Ekaterina Sherstyukova, Snezhanna Kandrashina, Vladimir Inozemtsev, Viktoria Sergunova

{"title":"Atomic Force Microscopy and Scanning Ion-Conductance Microscopy for Investigation of Biomechanical Characteristics of Neutrophils.","authors":"Mikhail Shvedov, Ekaterina Sherstyukova, Snezhanna Kandrashina, Vladimir Inozemtsev, Viktoria Sergunova","doi":"10.3390/cells13211757","DOIUrl":"10.3390/cells13211757","url":null,"abstract":"Scanning probe microscopy (SPM) is a versatile tool for studying a wide range of materials. It is well suited for investigating living matter, for example, in single-cell neutrophil studies. SPM has been extensively utilized to analyze cell physical properties, providing detailed insights into their structural and functional characteristics at the nanoscale. Its long-standing application in this field highlights its essential role in cell biology and immunology research, significantly contributing to understanding cellular mechanics and interactions. In this review, we discuss the application of SPM techniques, specifically atomic force microscopy (AFM) and scanning ion-conductance microscopy (SICM), to study the fundamental functions of neutrophils. In addition, recent advances in the application of SPM in single-cell immunology are discussed. The application of these techniques allows for obtaining data on the morphology, topography, and mechanical and electrochemical properties of neutrophils with high accuracy.","PeriodicalId":9743,"journal":{"name":"Cells","volume":"13 21","pages":""},"PeriodicalIF":8.3,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11545488/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142602993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Using Zebrafish to Study Multiciliated Cell Development and Disease States. 利用斑马鱼研究多纤毛细胞发育和疾病状态。

IF 8.3 2区生物学

Cells Pub Date : 2024-10-23 DOI: 10.3390/cells13211749

Thanh Khoa Nguyen, Sophia Baker, John-Michael Rodriguez, Liana Arceri, Rebecca A Wingert

{"title":"Using Zebrafish to Study Multiciliated Cell Development and Disease States.","authors":"Thanh Khoa Nguyen, Sophia Baker, John-Michael Rodriguez, Liana Arceri, Rebecca A Wingert","doi":"10.3390/cells13211749","DOIUrl":"10.3390/cells13211749","url":null,"abstract":"Multiciliated cells (MCCs) serve many important functions, including fluid propulsion and chemo- and mechanosensing. Diseases ranging from rare conditions to the recent COVID-19 global health pandemic have been linked to MCC defects. In recent years, the zebrafish has emerged as a model to investigate the biology of MCCs. Here, we review the major events in MCC formation including centriole biogenesis and basal body docking. Then, we discuss studies on the role of MCCs in diseases of the brain, respiratory, kidney and reproductive systems, as well as recent findings about the link between MCCs and SARS-CoV-2. Next, we explore why the zebrafish is a useful model to study MCCs and provide a comprehensive overview of previous studies of genetic components essential for MCC development and motility across three major tissues in the zebrafish: the pronephros, brain ependymal cells and nasal placode. Taken together, here we provide a cohesive summary of MCC research using the zebrafish and its future potential for expanding our understanding of MCC-related disease states.","PeriodicalId":9743,"journal":{"name":"Cells","volume":"13 21","pages":""},"PeriodicalIF":8.3,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11545745/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142603470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ketone Esters Partially and Selectively Rescue Mitochondrial Bioenergetics After Acute Cervical Spinal Cord Injury in Rats: A Time-Course. 酮酯类物质可选择性地部分修复大鼠急性颈脊髓损伤后的线粒体生物能：时间历程

IF 8.3 2区生物学

Cells Pub Date : 2024-10-22 DOI: 10.3390/cells13211746

Oscar Seira, HyoJoon David Park, Jie Liu, Michelle Poovathukaran, Kieran Clarke, Robert Boushel, Wolfram Tetzlaff

{"title":"Ketone Esters Partially and Selectively Rescue Mitochondrial Bioenergetics After Acute Cervical Spinal Cord Injury in Rats: A Time-Course.","authors":"Oscar Seira, HyoJoon David Park, Jie Liu, Michelle Poovathukaran, Kieran Clarke, Robert Boushel, Wolfram Tetzlaff","doi":"10.3390/cells13211746","DOIUrl":"10.3390/cells13211746","url":null,"abstract":"Spinal cord injury (SCI) pathology and pathophysiology can be attributed to both primary physical injury and secondary injury cascades. Secondary injury cascades involve dysregulated metabolism and energetic deficits directly linked to compromised mitochondrial bioenergetics. Rescuing mitochondrial function and reducing oxidative stress are associated with neuroprotection. In this regard, ketosis after traumatic brain injury (TBI), or after SCI, improves secondary neuropathology by decreasing oxidative stress, increasing antioxidants, reducing inflammation, and improving mitochondrial bioenergetics. Here, we follow up on our previous study and have used an exogenous ketone monoester, (R)-3-hydroxybutyl (R)-3-hydroxybutyrate (KE), as an alternative to a ketogenic diet, focusing on mitochondrial function between 1 and 14 days after injury. Starting 3 h following a cervical level 5 (C5) hemi-contusion injury, animals were fed either a standard control diet (SD) or a ketone ester diet (KED) combined with KE administered orally (OKE). We found that mitochondrial function was reduced after SCI at all times post-SCI, accompanied by reduced expression of most of the components of the electron transport chain (ETC). The KE rescued some of the bioenergetic parameters 1 day after SCI when D-β-Hydroxybutyrate (BHB) concentrations were ~2 mM. Still, most of the beneficial effects were observed 14 days after injury, with BHB concentrations reaching values of 4-6 mM. To our knowledge, this is the first report to show the beneficial effects of KE in rescuing mitochondrial function after SCI and demonstrates the suitability of KE in ameliorating the metabolic dysregulation that occurs after traumatic SCI without requiring a restrictive dietary regime.","PeriodicalId":9743,"journal":{"name":"Cells","volume":"13 21","pages":""},"PeriodicalIF":8.3,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11545339/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142603159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Generation and Characterization of Three Novel Mouse Mutant Strains Susceptible to Audiogenic Seizures. 三种易受听源性癫痫发作影响的新型小鼠突变品系的产生和特征。

IF 8.3 2区生物学

Cells Pub Date : 2024-10-22 DOI: 10.3390/cells13211747

Elena G Varlamova, Vera P Kuldaeva, Natalia N Mitina, Maria S Gavrish, Elena V Kondakova, Victor S Tarabykin, Alexei A Babaev, Egor A Turovsky

{"title":"Generation and Characterization of Three Novel Mouse Mutant Strains Susceptible to Audiogenic Seizures.","authors":"Elena G Varlamova, Vera P Kuldaeva, Natalia N Mitina, Maria S Gavrish, Elena V Kondakova, Victor S Tarabykin, Alexei A Babaev, Egor A Turovsky","doi":"10.3390/cells13211747","DOIUrl":"10.3390/cells13211747","url":null,"abstract":"The mechanisms of epileptogenesis after brain injury, ischemic stroke, or brain tumors have been extensively studied. As a result, many effective antiseizure drugs have been developed. However, there are still many patients who are resistant to therapy. The molecular and genetic bases regarding such drug-resistant seizures have been poorly elucidated. In many cases, heavy seizures are instigated by brain development malformations and often caused by gene mutations. Such malformations can be demonstrated in mouse models by generating mutant strains. One of the most potent mutagens is ENU (N-ethyl-N-nitrosourea). In the present study, we describe three novel mutant strains generated by ENU-directed mutagenesis. Two of these strains present a very strong epileptic phenotype triggered by audiogenic stimuli (G9-1 and S5-1 strains). The third mouse strain is characterized by behavioral disorders and hyperexcitation of neuronal networks. We identified changes in the expression of those genes encoding neurotransmission proteins in the cerebral cortexes of these mice. It turned out that the G9-1 strain demonstrated the strongest disruptions in the expression of those genes encoding plasma membrane channels, excitatory glutamate receptors, and protein kinases. On the other hand, the number of GABAergic neurons was also affected by the mutation. All three lines are characterized by increased anxiety, excitability, and suppressed motor and orientational-exploratory activities. On the other hand, the strains with an epileptic phenotype-G9-1 and S5-1ave reduced learning ability, and the A9-2 mice line retains high learning ability.","PeriodicalId":9743,"journal":{"name":"Cells","volume":"13 21","pages":""},"PeriodicalIF":8.3,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11545774/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142603125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Analysis of Extracellular Vesicles from Cytotoxic CD8⁺ αβ T Cells and γδ T Cells. 细胞毒性 CD8+ αβ T 细胞和 γδ T 细胞胞外囊泡的比较分析

IF 5.1 2区生物学

Cells Pub Date : 2024-10-21 DOI: 10.3390/cells13201745

Lisa Griesel, Patrick Kaleja, Andreas Tholey, Marcus Lettau, Ottmar Janssen

{"title":"Comparative Analysis of Extracellular Vesicles from Cytotoxic CD8+ αβ T Cells and γδ T Cells.","authors":"Lisa Griesel, Patrick Kaleja, Andreas Tholey, Marcus Lettau, Ottmar Janssen","doi":"10.3390/cells13201745","DOIUrl":"https://doi.org/10.3390/cells13201745","url":null,"abstract":"Background: Although belonging to different branches of the immune system, cytotoxic CD8+ αβ T cells and γδ T cells utilize common cytolytic effectors including FasL, granzymes, perforin and granulysin. The effector proteins are stored in different subsets of lysosome-related effector vesicles (LREVs) and released to the immunological synapse upon target cell encounter. Notably, in activated cells, LREVs and potentially other vesicles are continuously produced and released as extracellular vesicles (EVs). Presumably, EVs serve as mediators of intercellular communication in the local microenvironment or at distant sites.Methods: EVs of activated and expanded cytotoxic CD8+ αβ T cells or γδ T cells were enriched from culture supernatants by differential and ultracentrifugation and characterized by nanoparticle tracking analyses and Western blotting. For a comparative proteomic profiling, EV preparations from both cell types were isobaric labeled with tandem mass tags (TMT10plex) and subjected to mass spectrometry analysis.Results: 686 proteins were quantified in EV preparations of cytotoxic CD8+ αβ T cells and γδ T cells. Both populations shared a major set of similarly abundant proteins, while much fewer proteins presented higher abundance levels in either CD8+ αβ T cells or γδ T cells. To our knowledge, we provide the first comparative analysis of EVs from cytotoxic CD8+ αβ T cells and γδ T cells.","PeriodicalId":9743,"journal":{"name":"Cells","volume":"13 20","pages":""},"PeriodicalIF":5.1,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11506423/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142495933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Szymanska et al. The Effect of Visfatin on the Functioning of the Porcine Pituitary Gland: An In Vitro Study. Cells 2023, 12, 2835. 更正：Szymanska et al.《Visfatin 对猪垂体功能的影响》：体外研究。细胞 2023，12，2835。

IF 5.1 2区生物学

Cells Pub Date : 2024-10-21 DOI: 10.3390/cells13201741

Karolina Szymanska, Edyta Rytelewska, Ewa Zaobidna, Marta Kiezun, Marlena Gudelska, Grzegorz Kopij, Kamil Dobrzyn, Ewa Mlyczynska, Patrycja Kurowska, Barbara Kaminska, Anna Nynca, Nina Smolinska, Agnieszka Rak, Tadeusz Kaminski

引用次数: 0

N⁶-Methyladenosine RNA Modification Regulates the Differential Muscle Development in Large White and Ningxiang Pigs. N6-甲基腺苷RNA修饰调控大白猪和宁乡猪的肌肉发育差异

IF 5.1 2区生物学

Cells Pub Date : 2024-10-21 DOI: 10.3390/cells13201744

Hao Gu, Kang Xu, Zhao Yu, Zufeng Ren, Fan Chen, Changfan Zhou, Wei Zeng, Hongyan Ren, Yulong Yin, Yanzhen Bi

{"title":"N6-Methyladenosine RNA Modification Regulates the Differential Muscle Development in Large White and Ningxiang Pigs.","authors":"Hao Gu, Kang Xu, Zhao Yu, Zufeng Ren, Fan Chen, Changfan Zhou, Wei Zeng, Hongyan Ren, Yulong Yin, Yanzhen Bi","doi":"10.3390/cells13201744","DOIUrl":"https://doi.org/10.3390/cells13201744","url":null,"abstract":"N6-methyladenosine (m6A) is the most common modification in eukaryotic RNAs. Growing research indicates that m6A methylation is crucial for a multitude of biological processes. However, research on the m6A modifications in the regulation of porcine muscle growth is lacking. In this study, we identified differentially expressed genes in the neonatal period of muscle development between Large White (LW) and NingXiang (NX) pigs and further reported m6A methylation patterns via MeRIP-seq. We found that m6A modification regulates muscle cell development, myofibrils, cell cycle, and phosphatase regulator activity during the neonatal phase of muscle development. Interestingly, differentially expressed genes in LW and NX pigs were mainly enriched in pathways involved in protein synthesis. Furthermore, we performed a conjoint analysis of MeRIP-seq and RNA-seq data and identified 27 differentially expressed and m6A-modified genes. Notably, a typical muscle-specific envelope transmembrane protein, WFS1, was differentially regulated by m6A modifications in LW and NX pigs. We further revealed that the m6A modification accelerated the degradation of WFS1 in a YTHDF2-dependent manner. Noteworthy, we identified a single nucleotide polymorphism (C21551T) within the last exon of WFS1 that resulted in variable m6A methylation, contributing to the differing WFS1 expression levels observed in LW and NX pigs. Our study conducted a comprehensive analysis of the m6A modification on NX and LW pigs during the neonatal period of muscle development, and elucidated the mechanism by which m6A regulates the differential expression of WFS1 in the two breeds.","PeriodicalId":9743,"journal":{"name":"Cells","volume":"13 20","pages":""},"PeriodicalIF":5.1,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11506082/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142495925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Asparagine614 Determines the Transport and Function of the Murine Anti-Aging Protein Klotho. 天冬酰胺 614 决定小鼠抗衰老蛋白 Klotho 的运输和功能

IF 5.1 2区生物学

Cells Pub Date : 2024-10-21 DOI: 10.3390/cells13201743

Zahra Fanaei-Kahrani, Christoph Kaether

{"title":"Asparagine614 Determines the Transport and Function of the Murine Anti-Aging Protein Klotho.","authors":"Zahra Fanaei-Kahrani, Christoph Kaether","doi":"10.3390/cells13201743","DOIUrl":"https://doi.org/10.3390/cells13201743","url":null,"abstract":"Klotho is an anti-aging protein whose deletion significantly reduces lifespan in mice, while its over-expression increases lifespan. Klotho is a type-I transmembrane protein that is N-glycosylated at eight positions within its ectodomain. Our study demonstrates that N-glycosylation or mutation at position N614, but not at N161, N285, or N346 in mouse Klotho, is critically involved in the transport of Klotho out of the endoplasmic reticulum (ER). Consequently, while wild-type Klotho-EGFP as well as the N-glycosylation mutants N161Q, N285Q, and N346Q were present at the plasma membrane (PM), only small amounts of the N614Q Klotho-EGFP were present at the PM, with most of the protein accumulating in the ER. Protein interactome analysis of Klotho-EGFP N614Q revealed increased interactions with proteasome-related proteins and proteins involved in ER protein processing, like heat shock proteins and protein disulfide isomerases, indicative of impaired protein folding. Co-immunoprecipitation experiments confirmed the interaction of Klotho-EGFP N614Q with ER chaperons. Interestingly, despite the low amounts of Klotho-EGFP N614Q at the PM, it efficiently induced FGF receptor-mediated ERK activation in the presence of FGF23, highlighting its efficacy in triggering downstream signaling, even in limited quantities at the PM.","PeriodicalId":9743,"journal":{"name":"Cells","volume":"13 20","pages":""},"PeriodicalIF":5.1,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11506777/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142495912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DNA-Binding Protein A Is Actively Secreted in a Calcium-and Inflammasome-Dependent Manner and Negatively Influences Tubular Cell Survival. DNA 结合蛋白 A 以钙和炎症依赖的方式主动分泌，并对肾小管细胞的存活产生负面影响。

IF 5.1 2区生物学

Cells Pub Date : 2024-10-21 DOI: 10.3390/cells13201742

Gregor Hoppstock, Jonathan A Lindquist, Antonia Willems, Annika Becker, Charlotte Reichardt, Ronnie Morgenroth, Saskia Stolze, Cheng Zhu, Sabine Brandt, Peter R Mertens

{"title":"DNA-Binding Protein A Is Actively Secreted in a Calcium-and Inflammasome-Dependent Manner and Negatively Influences Tubular Cell Survival.","authors":"Gregor Hoppstock, Jonathan A Lindquist, Antonia Willems, Annika Becker, Charlotte Reichardt, Ronnie Morgenroth, Saskia Stolze, Cheng Zhu, Sabine Brandt, Peter R Mertens","doi":"10.3390/cells13201742","DOIUrl":"https://doi.org/10.3390/cells13201742","url":null,"abstract":"DNA-binding protein A (DbpA) belongs to the Y-box family of cold shock domain (CSD) proteins that bind RNA/DNA and exert intracellular functions in cell stress, proliferation, and differentiation. Given the pattern of DbpA staining in inflammatory glomerular diseases, without adherence to cell boundaries, we hypothesized extracellular protein occurrence and specific functions. Lipopolysaccharide and ionomycin induce DbpA expression and secretion from melanoma and mesangial cells. Unlike its homologue Y-box-binding protein 1 (YB-1), DbpA secretion requires inflammasome activation, as secretion is blocked upon the addition of a NOD-like receptor protein-3 (NLRP3) inhibitor. The addition of recombinant DbpA enhances melanoma cell proliferation, migration, and competes with tumor necrosis factor (TNF) binding to its receptor (TNFR1). In TNF-induced cell death assays, rDbpA initially blocks TNF-induced apoptosis, whereas at later time points (>24 h), cells are more prone to die. Given that CSD proteins YB-1 and DbpA fulfill the criteria of alarmins, we propose that their release signals an inherent danger to the host. Some data hint at an extracellular complex formation at a ratio of 10:1 (DbpA:YB-1) of both proteins.","PeriodicalId":9743,"journal":{"name":"Cells","volume":"13 20","pages":""},"PeriodicalIF":5.1,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11506473/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142516268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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