Cell and Tissue Biology最新文献

{"title":"A Sequential Culturing System for Generating Epithelial-Like Stem Cells from Human Mesenchymal Stem Cells Derived from Adipose Tissue","authors":"Taki Tiraihi, Zeinab Najafi Gravari, Masoud Soleimani","doi":"10.1134/s1990519x23060147","DOIUrl":"https://doi.org/10.1134/s1990519x23060147","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Skin burn repair requires efficient regeneration in order to achieve proper healing. We applied a sequential culturing system 2D1w/3D2w protocol for differentiating human mesenvhymal stem cell derived from adipose tissue (hMSC-AT) into epithelial-like stem cells (ELSC). hMSC-AT were cultured, characterized by immunophenotyping, mesenchymal differentiation and induced in a 2D culture using epithelial differentiation medium containing ATRA, ITS, dexamethasone, EGF and FGF-2 for 7 days (2D1w), followed by seeding in a 3D culture using chitosan hydrogel combined with the same cocktail (3D2w) for 2 weeks. The immunostaining of the cells was done at different stages of induction in order to characterize the differentiated cells using pan cytokeratin and anti-cytokeratins 14 and 18 antibodies. The expression of cytokeratins 5, 10, 14, 18 and 19 was evaluated by RT-PCR. The toxicity of chitosan hydrogel and proliferation of ELSC in 3D chitosan hydrogel were evaluated by MTT test. The viability of ELSC in 3D culture was evaluated by acridine orange/propidium iodide staining. The hMSC-AT were immunopositive to CD90, CD105, CD73, CD45 and CD34. The ELSC in the 2D culture expressed cytokeratin 19 after 7 days, while the other cytokeratins were not expressed. At the second week (3D culture), all of the above markers were expressed except cytokerstin10, moreover, the viability and proliferation were 98.02 ± 1.34 and 5.45 ± 0.36%, respectively. The cytotoxicity assay demonstrates the biocompatibility of chitosan hydrogel. The study reveals that hMSC-AT, seeded on chitosan hydrogel, can be induced into ELSC in the presence of other inducers.</p>","PeriodicalId":9705,"journal":{"name":"Cell and Tissue Biology","volume":"88 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138574691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Features of Matrix Metalloproteinases MMP2, MMP3, and MMP9 of Regulatory-Region Polymorphism in Patients with Uterine Fibroids","authors":"A. V. Shevchenko, V. F. Prokof’ev, V. I. Konenkov, Yu. S. Timofeeva, E. G. Koroleva, I. O. Marinkin, S. V. Aidagulova","doi":"10.1134/s1990519x23060135","DOIUrl":"https://doi.org/10.1134/s1990519x23060135","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Dysregulation of the extracellular matrix components synthesis contributes to the formation and growth of uterine fibroids. Changes of collagen metabolism in connective tissue may be associated with polymorphism of matrix metalloproteinase (MMP) genes. The aim of the study was to analyze of the association of regulatory regions of matrix metalloproteinase <i>MMP2</i> (rs243865), <i>MMP3</i> (rs3025058), and <i>MMP9</i> (rs3918242) genes with the development of leiomyoma, its histological form, and several concomitant gynecological diseases. A clinical study of 69 patients (23–54 years old) with myomas was conducted. According to the anamnesis, 57.9% of patients had given birth, 46.4% of women had had an artificial interruption of pregnancy, and 15.9% of women had had endometriosis. In histological examination, in 48.14% of cases, the nodes corresponded to the phenotype of simple fibroids with a large proportion of fibrous tissue, while 51.6% corresponded to the phenotype of proliferating fibroids. The comparison group is represented by a random population sample of women from Western Siberia. A total of 183 women without pronounced gynecological diseases were examined. <i>MMP2-1306</i> <i>C/T</i> polymorphism was analyzed by TaqMan, <i>MMP3-1171 5A/6A</i>, and <i>MMP9-1562 C/T</i> by restriction fragment length polymorphism method. The genotype frequencies of the analyzed genes did not significantly differ between the groups. The complex genotype <i>MMP2-1306CC:MMP3-11715A6A:MMP9-1562CT</i> was decreased in women with myoma relative to the persons of the comparison group. In endometriosis patients, the <i>MMP9-1562CC</i> genotype was reduced and heterozygosity was increased relative to patients without endometriosis. The frequency of the <i>MMP2-1306CC:MMP9-1562CT</i> complex genotype is significantly higher in women who gave birth than in women who did not give birth. Differences in frequencies of complex genotypes between histological variants of leiomyoma were revealed. The results of the study show the significance of polymorphism effect of the regulatory regions of the <i>MMP</i> genes in the development of leiomyoma and nature of disease progression.</p>","PeriodicalId":9705,"journal":{"name":"Cell and Tissue Biology","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138575021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Response of the Cell Genome of Endometrial Mesenchymal Stem Cells to the Procedure of Long-Term Cyropreservation","authors":"T. M. Grinchuk, M. A. Shorokhova, N. A. Pugovkina","doi":"10.1134/s1990519x2306007x","DOIUrl":"https://doi.org/10.1134/s1990519x2306007x","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Information about the effect of cryopreservation on cellular functions and the genetic apparatus of cells of differing genesis is not unambiguous and is in the process of accumulation. This work is aimed at studying the effect of long-term storage (7 years) in the frozen state of human endometrial mesenchymal stem cells (eMSCs) on the stability of their genome in vitro. The results showed destabilization of the karyotype structure in the descendants of cells after their thawing, namely, aneupolyploidization of the chromosome set; increased fragility of chromosomes, resulting in a huge pool of aberrant chromosomes; and impaired condensation in homologues. Chromosomal breakdowns affecting the centromeric regions were in some cases accompanied by the preservation of genetic material in the form of independent chromosomes. Almost all chromosomes of the set were involved in the process of destabilization of the eMSC cell genome. It has been shown that the procedure of long-term cryopreservation can become an inducer of premature cellular aging of eMSCs after their thawing. Comparison of the data obtained with the results of karyotyping of transformed Chinese hamster cells that underwent a similar procedure led to the conclusion that cryopreservation for biological systems can be a stress that induces heterogeneous genetic defects at the karyotype level. The response of the genome of cells of different origin to the same conditions of cryopreservation may differ.</p>","PeriodicalId":9705,"journal":{"name":"Cell and Tissue Biology","volume":"105 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138574619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Investigation of the Regulatory Relationship of the Keap1/Nrf2/ARE Signaling System and Transcriptional Regulators of Lysosomal Biogenesis","authors":"A. V. Chechushkov, E. B. Menshchikova","doi":"10.1134/s1990519x23060056","DOIUrl":"https://doi.org/10.1134/s1990519x23060056","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Despite the key role of the Keap1/Nrf2/ARE redox-sensitive signaling system in cell metabolism, almost nothing is known about its association with lysosome biogenesis. In the present work, a theoretical and experimental analysis of the possibility of such a relationship is carried out. By forming a position frequency matrix in the genes of the transcription factors TFEB and TFE3, the presence of a large number of ARE-like sequences in noncoding regions was found. The impact in vitro on J774 cells with activators of the Keap1/Nrf2/ARE system (original synthetic monophenol TS-13 and reference drug <i>tert</i>-butylhydroquinone) leads to dose-dependent induction of <i>Tfe3</i> and <i>Tfeb</i> genes accompanied by a gradual increase in the number of lysosomes and the intensity of the autophagosome–lysosome fusion. Thus, it can be assumed that proteins controlling ARE-dependent genes can influence lysosomal biogenesis.</p>","PeriodicalId":9705,"journal":{"name":"Cell and Tissue Biology","volume":"69 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138577155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Mechanisms Underlying Alzheimer’s and Parkinson’s Disease and the Possibility of Their Neutralization","authors":"O. V. Nevzglyadova, E. V. Mikhailova, T. R. Soidla","doi":"10.1134/S1990519X23060093","DOIUrl":"https://doi.org/10.1134/S1990519X23060093","url":null,"abstract":"","PeriodicalId":9705,"journal":{"name":"Cell and Tissue Biology","volume":"26 3‐4","pages":"593 - 607"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138626270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The History of the Study of the Neural Crest (Overview)","authors":"N. Yu. Pakhomova, E. L. Strokova, A. A. Korytkin, V. V. Kozhevnikov, A. F. Gusev, A. M. Zaidman","doi":"10.1134/s1990519x23050097","DOIUrl":"https://doi.org/10.1134/s1990519x23050097","url":null,"abstract":"","PeriodicalId":9705,"journal":{"name":"Cell and Tissue Biology","volume":"118 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136060483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Titanium-Dioxide Nanoparticles Suppress Entosis in MCF-7 Human Breast Adenocarcinoma Culture","authors":"O. P. Kisurina-Evgenieva, M. A. Savitskaya, D. S. Smeshnova, G. E. Onishchenko","doi":"10.1134/s1990519x23050073","DOIUrl":"https://doi.org/10.1134/s1990519x23050073","url":null,"abstract":"","PeriodicalId":9705,"journal":{"name":"Cell and Tissue Biology","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136061677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of 3D Scaffolds in Tissue Engineering","authors":"Seyedeh Mahdieh Khoshnazar, Asadollah Asadi, Saman Roshancheshm, Aida Karimian, Arash Abdolmaleki","doi":"10.1134/s1990519x23050061","DOIUrl":"https://doi.org/10.1134/s1990519x23050061","url":null,"abstract":"","PeriodicalId":9705,"journal":{"name":"Cell and Tissue Biology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136060498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synaptophysin Expression by Supraependymal Structures of Rat Brain","authors":"V. A. Razenkova, O. V. Kirik","doi":"10.1134/s1990519x23050115","DOIUrl":"https://doi.org/10.1134/s1990519x23050115","url":null,"abstract":"","PeriodicalId":9705,"journal":{"name":"Cell and Tissue Biology","volume":"54 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136060819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Role of the Integrated Response of Tumor Cells to Stress, Autophagy, and Chaperones in the Origin of Recurrent Resistant Tumors","authors":"S. G. Zubova, O. O. Gnedina","doi":"10.1134/s1990519x23050139","DOIUrl":"https://doi.org/10.1134/s1990519x23050139","url":null,"abstract":"","PeriodicalId":9705,"journal":{"name":"Cell and Tissue Biology","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136060176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}