{"title":"The Response of the Cell Genome of Endometrial Mesenchymal Stem Cells to the Procedure of Long-Term Cyropreservation","authors":"T. M. Grinchuk, M. A. Shorokhova, N. A. Pugovkina","doi":"10.1134/s1990519x2306007x","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Information about the effect of cryopreservation on cellular functions and the genetic apparatus of cells of differing genesis is not unambiguous and is in the process of accumulation. This work is aimed at studying the effect of long-term storage (7 years) in the frozen state of human endometrial mesenchymal stem cells (eMSCs) on the stability of their genome in vitro. The results showed destabilization of the karyotype structure in the descendants of cells after their thawing, namely, aneupolyploidization of the chromosome set; increased fragility of chromosomes, resulting in a huge pool of aberrant chromosomes; and impaired condensation in homologues. Chromosomal breakdowns affecting the centromeric regions were in some cases accompanied by the preservation of genetic material in the form of independent chromosomes. Almost all chromosomes of the set were involved in the process of destabilization of the eMSC cell genome. It has been shown that the procedure of long-term cryopreservation can become an inducer of premature cellular aging of eMSCs after their thawing. Comparison of the data obtained with the results of karyotyping of transformed Chinese hamster cells that underwent a similar procedure led to the conclusion that cryopreservation for biological systems can be a stress that induces heterogeneous genetic defects at the karyotype level. The response of the genome of cells of different origin to the same conditions of cryopreservation may differ.</p>","PeriodicalId":9705,"journal":{"name":"Cell and Tissue Biology","volume":"105 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cell and Tissue Biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1134/s1990519x2306007x","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
引用次数: 0
Abstract
Information about the effect of cryopreservation on cellular functions and the genetic apparatus of cells of differing genesis is not unambiguous and is in the process of accumulation. This work is aimed at studying the effect of long-term storage (7 years) in the frozen state of human endometrial mesenchymal stem cells (eMSCs) on the stability of their genome in vitro. The results showed destabilization of the karyotype structure in the descendants of cells after their thawing, namely, aneupolyploidization of the chromosome set; increased fragility of chromosomes, resulting in a huge pool of aberrant chromosomes; and impaired condensation in homologues. Chromosomal breakdowns affecting the centromeric regions were in some cases accompanied by the preservation of genetic material in the form of independent chromosomes. Almost all chromosomes of the set were involved in the process of destabilization of the eMSC cell genome. It has been shown that the procedure of long-term cryopreservation can become an inducer of premature cellular aging of eMSCs after their thawing. Comparison of the data obtained with the results of karyotyping of transformed Chinese hamster cells that underwent a similar procedure led to the conclusion that cryopreservation for biological systems can be a stress that induces heterogeneous genetic defects at the karyotype level. The response of the genome of cells of different origin to the same conditions of cryopreservation may differ.
期刊介绍：
The journal publishes papers on vast aspects of cell research, including morphology, biochemistry, biophysics, genetics, molecular biology, immunology. The journal accepts original experimental studies, theoretical articles suggesting novel principles and approaches, presentations of new hypotheses, reviews highlighting major developments in cell biology, discussions. The main objective of the journal is to provide a competent representation and integration of research made on cells (animal and plant cells, both in vivo and in cell culture) offering insight into the structure and functions of live cells as a whole. Characteristically, the journal publishes articles on biology of free-living and parasitic protists, which, unlike Metazoa, are eukaryotic organisms at the cellular level of organization.
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