Canadian journal of biochemistry and cell biology = Revue canadienne de biochimie et biologie cellulaire最新文献_第8页

Method comparison for analyzing wound healing rates. 方法比较分析创面愈合率。

Canadian journal of biochemistry and cell biology = Revue canadienne de biochimie et biologie cellulaire Pub Date : 2017-01-30 DOI: 10.1139/bcb-2016-0163

Prabhpreet K Dhillon, Xinyin Li, J. Sanes, Oluwafemi Akintola, Bingyun Sun

引用次数: 2

Differential effect of hypoxia and acidity on lung cancer cell and fibroblast metabolism. 缺氧和酸性对肺癌细胞和成纤维细胞代谢的差异影响。

Canadian journal of biochemistry and cell biology = Revue canadienne de biochimie et biologie cellulaire Pub Date : 2017-01-24 DOI: 10.1139/bcb-2016-0197

A. Giatromanolaki, Maria Liousia, Stella Arelaki, D. Kalamida, S. Pouliliou, A. Mitrakas, A. Tsolou, E. Sivridis, M. Koukourakis

{"title":"Differential effect of hypoxia and acidity on lung cancer cell and fibroblast metabolism.","authors":"A. Giatromanolaki, Maria Liousia, Stella Arelaki, D. Kalamida, S. Pouliliou, A. Mitrakas, A. Tsolou, E. Sivridis, M. Koukourakis","doi":"10.1139/bcb-2016-0197","DOIUrl":"https://doi.org/10.1139/bcb-2016-0197","url":null,"abstract":"This study examined the metabolic response of lung cancer cells and normal lung fibroblasts to hypoxia and acidity. GLUT1 and HXKII mRNA/protein expression was up-regulated under hypoxia in the MRC5 fibroblasts and in the A549 and H1299 lung cancer cell lines, indicating intensified glucose absorption and glycolysis. Under hypoxia, the LDHA mRNA and LDH5 protein levels increased in the cancer cells but not in the fibroblasts. Acidity suppressed the above-mentioned hypoxia effect. PDH-kinase-1 (PDK1 mRNA and protein) and inactive phosphorylated-PDH protein levels were induced under hypoxia in the cancer cells, whereas these were reduced in the MRC5 lung fibroblasts. In human tissue sections, the prevalent expression patterns supported the contrasting metabolic behavior of cancer cells vs. tumor fibroblasts. The monocarboxylate/lactate transporter 1 (MCT1) was up-regulated in all the cell lines under hypoxic conditions, but it was suppressed under acidic conditions. The mitochondrial DNA (mtDNA) content per cell decreased significantly in the A549 cancer cell line under hypoxia, but it increased in the MRC5 fibroblasts. Taking into account these findings, we suggest that, under hypoxia, cancer cells intensify the anaerobic direction in glycolysis, while normal fibroblasts prefer to seek energy by intensifying the aerobic use of the available oxygen.","PeriodicalId":9524,"journal":{"name":"Canadian journal of biochemistry and cell biology = Revue canadienne de biochimie et biologie cellulaire","volume":"24 1","pages":"428-436"},"PeriodicalIF":0.0,"publicationDate":"2017-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81756512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Advances in lactoferrin research concerning bovine mastitis. 乳铁蛋白在奶牛乳腺炎中的研究进展。

Canadian journal of biochemistry and cell biology = Revue canadienne de biochimie et biologie cellulaire Pub Date : 2017-01-20 DOI: 10.1139/bcb-2016-0044

K. Shimazaki, K. Kawai

{"title":"Advances in lactoferrin research concerning bovine mastitis.","authors":"K. Shimazaki, K. Kawai","doi":"10.1139/bcb-2016-0044","DOIUrl":"https://doi.org/10.1139/bcb-2016-0044","url":null,"abstract":"Lactoferrin is a multifunctional, iron-binding glycoprotein found in milk and other exocrine secretions. Lactoferrin in milk plays vital roles in the healthy development of newborn mammals, and is also an innate resistance factor involved in the prevention of mammary gland infection by microorganisms. Inflammation of the udder because of bacterial infection is referred to as mastitis. There have been many investigations into the relationships between lactoferrin and mastitis, which fall into several categories. The main categories are fluctuations in the lactoferrin concentration of milk, lactoferrin activity against mastitis pathogens, elucidation of the processes underlying the onset of mastitis, participation of lactoferrin in the immune system, and utilization of lactoferrin in mastitis treatment and prevention. This minireview describes lactoferrin research concerning bovine mastitis. In the 1970s, many researchers reported that the lactoferrin concentration fluctuates in milk from cows with mastitis. From the late 1980s, many studies clarified the infection-defense mechanism in the udder and the contribution of lactoferrin to the immune system. After the year 2000, the processes underlying the onset of mastitis were elucidated in vivo and in vitro, and lactoferrin was applied for the treatment and prevention of mastitis.","PeriodicalId":9524,"journal":{"name":"Canadian journal of biochemistry and cell biology = Revue canadienne de biochimie et biologie cellulaire","volume":"3 1","pages":"69-75"},"PeriodicalIF":0.0,"publicationDate":"2017-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80566177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 33

Lactoferrin inhibits melanogenesis by down-regulating MITF in melanoma cells and normal melanocytes. 乳铁蛋白通过下调黑色素瘤细胞和正常黑色素细胞的MITF抑制黑色素生成。

Canadian journal of biochemistry and cell biology = Revue canadienne de biochimie et biologie cellulaire Pub Date : 2017-01-19 DOI: 10.1139/bcb-2016-0053

Nanase Ishii, M. Ryu, Yasushi A. Suzuki

{"title":"Lactoferrin inhibits melanogenesis by down-regulating MITF in melanoma cells and normal melanocytes.","authors":"Nanase Ishii, M. Ryu, Yasushi A. Suzuki","doi":"10.1139/bcb-2016-0053","DOIUrl":"https://doi.org/10.1139/bcb-2016-0053","url":null,"abstract":"The aim of this study was to evaluate the effect of bovine lactoferrin (bLf) on melanin-producing cells and to elucidate its mechanism of action. We tested the anti-melanogenic effect of bLf on a 3-dimensional cultured pigmentation skin model and confirmed a 20% reduction in pigmentation, suggesting that bLf was transdermally absorbed and it suppressed melanin production. Treatment of human melanoma cells with bLf resulted in a significant, dose-dependent suppression of melanin production. Apo-bLf and holo-bLf suppressed melanogenesis to the same degree as bLf. The key feature behind this anti-melanogenic effect of bLf was the down-regulation of the microphthalmia-associated transcription factor (MITF), leading to the suppression of tyrosinase activity. Treatment with bLf resulted in both decreased expression of MITF mRNA and enhanced degradation of MITF protein. However, the primary effector was enhanced phosphorylation of extracellular signal-regulated kinase (ERK), leading to the phosphorylation and degradation of MITF. Our finding that bLf suppresses melanin production in melanocytes indicates that bLf is a possible candidate for application as a skin-whitening agent.","PeriodicalId":9524,"journal":{"name":"Canadian journal of biochemistry and cell biology = Revue canadienne de biochimie et biologie cellulaire","volume":"C-27 1","pages":"119-125"},"PeriodicalIF":0.0,"publicationDate":"2017-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84433884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Protein-surfactant interactions between bovine lactoferrin and sophorolipids under neutral and acidic conditions. 中性和酸性条件下牛乳铁蛋白与槐磷脂之间的蛋白质-表面活性剂相互作用。

Canadian journal of biochemistry and cell biology = Revue canadienne de biochimie et biologie cellulaire Pub Date : 2017-01-19 DOI: 10.1139/bcb-2016-0057

Kentaro Matsumiya, Yasushi A. Suzuki, Y. Hirata, Yuko Nambu, Y. Matsumura

引用次数: 14

Ceruloplasmin-derived peptide is the strongest regulator of oxidative stress and leukotriene synthesis in neutrophils. 铜蓝蛋白衍生肽是中性粒细胞氧化应激和白三烯合成的最强调节剂。

Canadian journal of biochemistry and cell biology = Revue canadienne de biochimie et biologie cellulaire Pub Date : 2017-01-10 DOI: 10.1139/bcb-2016-0180

E. Golenkina, Alexey Livenskyi, G. Viryasova, Y. Romanova, G. Sud’ina, A. Sokolov

{"title":"Ceruloplasmin-derived peptide is the strongest regulator of oxidative stress and leukotriene synthesis in neutrophils.","authors":"E. Golenkina, Alexey Livenskyi, G. Viryasova, Y. Romanova, G. Sud’ina, A. Sokolov","doi":"10.1139/bcb-2016-0180","DOIUrl":"https://doi.org/10.1139/bcb-2016-0180","url":null,"abstract":"Ceruloplasmin, an acute-phase protein, can affect the activity of leukocytes through its various enzymatic activities and protein-protein interactions (with lactoferrin, myeloperoxidase, eosinophil peroxidase, serprocidins, and 5-lipoxygenase (5-LOX), among others). However, the molecular mechanisms of ceruloplasmin activity are not clearly understood. In this study, we tested the ability of two synthetic peptides, RPYLKVFNPR (883-892) (P1) and RRPYLKVFNPRR (882-893) (P2), corresponding to the indicated fragments of the ceruloplasmin sequence, to affect neutrophil activation. Leukotriene (LT) B4 is the primary eicosanoid product of polymorphonuclear leukocytes (PMNLs, neutrophils). We studied leukotriene synthesis in PMNLs upon interaction with Salmonella enterica serovar Typhimurium. Priming of neutrophils with phorbol 12-myristate 13-acetate (PMA) elicited the strong regulatory function of P2 peptide as a superoxide formation inducer and leukotriene synthesis inhibitor. Ceruloplasmin-derived P2 peptide appeared to be a strong inhibitor of 5-LOX product synthesis under conditions of oxidative stress.","PeriodicalId":9524,"journal":{"name":"Canadian journal of biochemistry and cell biology = Revue canadienne de biochimie et biologie cellulaire","volume":"100 1","pages":"445-449"},"PeriodicalIF":0.0,"publicationDate":"2017-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89922072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Use of substitute Nonidet P-40 nonionic detergents in intracellular tubulin polymerization assays for screening of microtubule targeting agents. 使用替代Nonidet P-40非离子洗涤剂在细胞内微管聚合试验中筛选微管靶向剂。

Canadian journal of biochemistry and cell biology = Revue canadienne de biochimie et biologie cellulaire Pub Date : 2017-01-10 DOI: 10.1139/bcb-2016-0141

S. Sinha, J. J. Field, J. H. Miller

引用次数: 12

Regular papers / Articles ordinaires. 普通报纸/普通物品。

Canadian journal of biochemistry and cell biology = Revue canadienne de biochimie et biologie cellulaire Pub Date : 2017-01-01 DOI: 10.1139/bcb-2017-0051

引用次数: 0

Special issue: Highlights of the 2015 Asilomar Chromatin, Chromosomes, and Epigenetics Conference / Numéro spécial : Points saillants de la Conférence 2015 d'Asilomar sur la chromatine, les chromosomes et l'épigénétique. 特刊:2015年Asilomar染色质、染色体和表观遗传学会议亮点/特刊:2015年Asilomar染色质、染色体和表观遗传学会议亮点。

Canadian journal of biochemistry and cell biology = Revue canadienne de biochimie et biologie cellulaire Pub Date : 2017-01-01 DOI: 10.1139/bcb-2017-0049

引用次数: 0

Special issue: Discovery and development of the CRISPR-Cas9 system / Numéro spécial : Découverte et mise au point du système CRISPR-Cas9. 特刊:CRISPR-Cas9系统的发现与发展/特刊:CRISPR-Cas9系统的发现与发展。

Canadian journal of biochemistry and cell biology = Revue canadienne de biochimie et biologie cellulaire Pub Date : 2017-01-01 DOI: 10.1139/bcb-2017-0050

引用次数: 0