中性和酸性条件下牛乳铁蛋白与槐磷脂之间的蛋白质-表面活性剂相互作用。

Canadian journal of biochemistry and cell biology = Revue canadienne de biochimie et biologie cellulaire Pub Date : 2017-01-19 DOI:10.1139/bcb-2016-0057

Kentaro Matsumiya, Yasushi A. Suzuki, Y. Hirata, Yuko Nambu, Y. Matsumura

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引用次数: 14

摘要

为了了解天然衍生的sophorolipids (SLs)和牛乳铁蛋白(bLf)之间的蛋白质-表面活性剂相互作用，我们在弱酸性和中性pH条件下进行了光谱、显微镜和生化实验。粒度分析、显微镜观察和酶解分析表明，在pH 5.0和7.0条件下，bLf和SLs相互作用形成片状和小聚集结构，反映了SLs的原始自组织结构。圆二色性(CD)表明，SLs对bLf的二级结构没有显著影响。

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Protein-surfactant interactions between bovine lactoferrin and sophorolipids under neutral and acidic conditions.

To understand the protein-surfactant interactions between naturally derived sophorolipids (SLs) and bovine lactoferrin (bLf), we carried out spectroscopic, microscopic, and biochemical experiments under weakly acidic and neutral pH conditions. Particle size analysis, microscopy, and enzymatic digestion indicated that bLf and SLs interact with each other to form sheet-like and small aggregated structures reflecting the original self-organization of SLs at pH 5.0 and 7.0, respectively. Circular dichroism (CD) showed that SLs did not significantly affect the secondary structure of bLf.

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Canadian journal of biochemistry and cell biology = Revue canadienne de biochimie et biologie cellulaire

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