发布求助
文献互助 智能选刊 最新文献

Canadian journal of biochemistry最新文献

筛选
英文 中文
Effects of membrane fluidity and identification of the rate-limiting step in the protein-mediated phosphatidylcholine exchange reaction. 膜流动性对蛋白介导的磷脂酰胆碱交换反应的影响及限速步骤的鉴定。
Canadian journal of biochemistry Pub Date : 1982-04-01 DOI: 10.1139/o82-048
R P Bozzato, D O Tinker
{"title":"Effects of membrane fluidity and identification of the rate-limiting step in the protein-mediated phosphatidylcholine exchange reaction.","authors":"R P Bozzato, D O Tinker","doi":"10.1139/o82-048","DOIUrl":"https://doi.org/10.1139/o82-048","url":null,"abstract":"","PeriodicalId":9508,"journal":{"name":"Canadian journal of biochemistry","volume":" ","pages":"409-18"},"PeriodicalIF":0.0,"publicationDate":"1982-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1139/o82-048","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40718568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 22
Comparative sequence analysis as an approach to evaluating structure, function, and evolution of 5S and 5.8S ribosomal RNAs. 比较序列分析作为评价5S和5.8S核糖体rna结构、功能和进化的方法。
Canadian journal of biochemistry Pub Date : 1982-04-01 DOI: 10.1139/o82-057
R M MacKay, D F Spencer, M N Schnare, W F Doolittle, M W Gray
{"title":"Comparative sequence analysis as an approach to evaluating structure, function, and evolution of 5S and 5.8S ribosomal RNAs.","authors":"R M MacKay,&nbsp;D F Spencer,&nbsp;M N Schnare,&nbsp;W F Doolittle,&nbsp;M W Gray","doi":"10.1139/o82-057","DOIUrl":"https://doi.org/10.1139/o82-057","url":null,"abstract":"<p><p>Nucleotide sequences of nine eukaryotic and nine eubacterial 5S rRNAs have been selected for their diversity and subjected to analysis of primary and potential secondary structure. This analysis has allowed the quantitative confirmation of several previously made observations concerning 5S rRNA structure: (i) these two 5S rRNAs are derived from a common ancestor and probably perform essentially the same function in protein synthesis; (ii) one domain of 5S rRNA has undergone considerable divergence of structure (and presumably function) since the separation of the eukaryotic and eubacterial lineages; and (iii) single-stranded regions are more highly conserved than double-stranded regions. In addition, this analysis leads us to propose that (i) some of the highly conserved nucleotide residues in single-stranded regions interact in a specific manner with protein components of the translational apparatus, and (ii) repetitive folding and unfolding of helical regions occurs in two regions of eukaryotic 5S rRNA and one region of eubacterial 5S rRNA. In the context of these observations and propositions we also consider the potential secondary structure of plant mitochondrial 5S rRNA. Nucleotide sequences of 5.85 rRNAs have yielded less information about secondary structure and possible functional interactions. However, we have identified highly conserved and variable regions within this molecule and we show (in contrast to the situation with 5S rRNA) that these do not correlate well with proposed single-stranded and helical regions in a current model of 5.8S secondary structure.</p>","PeriodicalId":9508,"journal":{"name":"Canadian journal of biochemistry","volume":" ","pages":"480-9"},"PeriodicalIF":0.0,"publicationDate":"1982-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1139/o82-057","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40718375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 16
Rat liver ribonucleotide reductase: separation, purification, and properties of two nonidentical subunits. 大鼠肝核糖核苷酸还原酶:两个不同亚基的分离、纯化和性质。
Canadian journal of biochemistry Pub Date : 1982-04-01 DOI: 10.1139/o82-054
T Youdale, J P MacManus, J F Whitfield
{"title":"Rat liver ribonucleotide reductase: separation, purification, and properties of two nonidentical subunits.","authors":"T Youdale,&nbsp;J P MacManus,&nbsp;J F Whitfield","doi":"10.1139/o82-054","DOIUrl":"https://doi.org/10.1139/o82-054","url":null,"abstract":"<p><p>Two nonidentical subunits of mammalian ribonucleotide reductase, L1 and L2, from regenerating rat liver have been extensively purified for the first time. They were separated by dATP-Sepharose affinity chromatography. Subunit L1, which bound to dATP-Sepharose, was eluted with 50 mM ATP and purified to homogeneity (as demonstrated by sodium dodecyl sulfate (SDS) - polyacrylamide gel electrophoresis) by molecular exclusion high-pressure liquid chromatography (HPLC). This subunit had an apparent relative mass (Mr) of 45 000 and a Km of 0.9 X 10(-4) for CDP. Subunit L2, which did not bind to dATP-Sepharose, was purified by pH 5.2 precipitation followed by chromatography on CM-Sephadex, molecular exclusion HPLC, and DEAE-cellulose. This subunit contained iron and had an apparent Mr of 120 000 by HPLC molecular exclusion chromatography, but showed two bands (Mr 75 000 and Mr 47 000) on SDS-polyacrylamide gel electrophoresis. Neither L1 nor L2 separately had any enzyme activity but when combined they reduced CDP to dCDP.</p>","PeriodicalId":9508,"journal":{"name":"Canadian journal of biochemistry","volume":"60 4","pages":"463-70"},"PeriodicalIF":0.0,"publicationDate":"1982-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1139/o82-054","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18096964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 24
Proteins and enzymes of the brush-border membrane of mouse intestine: influence of organ culture on gel electrophoretic patterns. 小鼠肠刷缘膜的蛋白质和酶:器官培养对凝胶电泳模式的影响。
Canadian journal of biochemistry Pub Date : 1982-04-01 DOI: 10.1139/o82-051
A Berteloot, J S Hugon
{"title":"Proteins and enzymes of the brush-border membrane of mouse intestine: influence of organ culture on gel electrophoretic patterns.","authors":"A Berteloot,&nbsp;J S Hugon","doi":"10.1139/o82-051","DOIUrl":"https://doi.org/10.1139/o82-051","url":null,"abstract":"<p><p>Purifications of mouse intestinal brush-border membranes from control explants and scrapings of intestinal mucosa have been compared. Based on the specific activity of sucrase used as a specific marker of these membranes, higher purification factors were obtained with control explants (24.7 +/- 0.9) as compared with scrapings of intestinal mucosa (14.8 +/- 0.9). However, similar patterns of proteins and enzymes were obtained by sodium dodecyl sulfate (SDS) - polyacrylamide gel electrophoresis after membrane solubilization by 2% SDS at room temperature. After 24 h of culture, higher molecular weight species of maltase-glucoamylase-isomaltase (band 4), alkaline phosphatase (bands 9-10), and trehalase (band 17) have been observed. Enzyme species appearing in the particulate fraction of culture media were, however, identical with those found at the brush-border membrane level in control explants, except for trehalase. These results are interpreted by considering the possible adsorption of serum components to brush-border membrane proteins. It thus appears that the membrane proteins and enzymes released in the media during organ culture are identical with those synthesized in the tissue in vitro or in vivo.</p>","PeriodicalId":9508,"journal":{"name":"Canadian journal of biochemistry","volume":" ","pages":"434-43"},"PeriodicalIF":0.0,"publicationDate":"1982-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1139/o82-051","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40718571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Expansion of phospholipid pool size of rat intestinal villus cells during fat absorption. 脂肪吸收过程中大鼠肠绒毛细胞磷脂池大小的扩大。
Canadian journal of biochemistry Pub Date : 1982-04-01 DOI: 10.1139/o82-052
N A Shaikh, A Kuksis
{"title":"Expansion of phospholipid pool size of rat intestinal villus cells during fat absorption.","authors":"N A Shaikh,&nbsp;A Kuksis","doi":"10.1139/o82-052","DOIUrl":"https://doi.org/10.1139/o82-052","url":null,"abstract":"<p><p>The effect of fat absorption upon the phospholipid pool size of the intestinal mucosal cells was determined in rats receiving fatty emulsions as a bolus by stomach tube or as multiple meals in the form of fat-laden laboratory chow. The phospholipid content of the mucosal scrapings and of the isolated villus cells was determined 3 to 34 h after the meals and was compared with the phospholipid content of cells from similar animals receiving water alone or 10% sucrose in water. It was shown that continuously fed animals averaged 5-10% and single meal fed animals up to 40% higher phospholipid content in their mucosal cells than the corresponding controls, when compared per milligram cell protein. The expansion of the phospholipid pool involved all phospholipid classes and correlated well with the phospholipid composition of prechylomicrons and of microsomal membranes, which undergo a significant proliferation during fat absorption. The apparent lower expansion of the phospholipid pool in the continuously fed animals correlated with the lower triacylglycerol content of the lumen and of the cells at these times.</p>","PeriodicalId":9508,"journal":{"name":"Canadian journal of biochemistry","volume":" ","pages":"444-51"},"PeriodicalIF":0.0,"publicationDate":"1982-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1139/o82-052","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40718572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
The 5S RNA - protein complex from yeast: a model for the evolution and structure of the eukaryotic ribosome. 酵母5S RNA -蛋白复合物:真核核糖体的进化和结构模型。
Canadian journal of biochemistry Pub Date : 1982-04-01 DOI: 10.1139/o82-058
R N Nazar, M Yaguchi, G E Willick
{"title":"The 5S RNA - protein complex from yeast: a model for the evolution and structure of the eukaryotic ribosome.","authors":"R N Nazar,&nbsp;M Yaguchi,&nbsp;G E Willick","doi":"10.1139/o82-058","DOIUrl":"https://doi.org/10.1139/o82-058","url":null,"abstract":"<p><p>The ribosomal 5S RNA - protein complex appears to be an excellent model for studies on the evolution and structure of ribosomes. In eukaryotes this complex is composed of two components, the 5S rRNA and a single ribosomal protein which in yeast has a molecular weight of about 38 000. The primary protein-binding site is located in the 3' -end region of the 5S RNA together with a small portion of the 5' end. The primary RNA-binding site appears to be situated in the C-terminal end of the protein (YL3 in yeast) but the binding specificity requires other structural elements in the N-terminal half of the molecule. When compared with prokaryotic 5S RNA - protein complexes, various physical and chemical studies suggest that the basic structure and interactions have been conserved in the course of evolution, but that the single larger eukaryotic 5S RNA binding protein has evolved through a fusion of genes for the multiple 5S RNA binding proteins in prokaryotes.</p>","PeriodicalId":9508,"journal":{"name":"Canadian journal of biochemistry","volume":"60 4","pages":"490-6"},"PeriodicalIF":0.0,"publicationDate":"1982-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1139/o82-058","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17244552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 16
Study of the evolution of the genetic code by comparing the structural and catalytic properties of the aminoacyl-tRNA synthetases. 通过比较氨基酰基- trna合成酶的结构和催化性质来研究遗传密码的进化。
Canadian journal of biochemistry Pub Date : 1982-04-01 DOI: 10.1139/o82-055
J Lapointe
{"title":"Study of the evolution of the genetic code by comparing the structural and catalytic properties of the aminoacyl-tRNA synthetases.","authors":"J Lapointe","doi":"10.1139/o82-055","DOIUrl":"https://doi.org/10.1139/o82-055","url":null,"abstract":"","PeriodicalId":9508,"journal":{"name":"Canadian journal of biochemistry","volume":" ","pages":"471-4"},"PeriodicalIF":0.0,"publicationDate":"1982-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1139/o82-055","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40718573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
A calorimetric study of the thermal transitions of Halobacterium cutirubrum. cutirubrum盐杆菌热转变的量热研究。
Canadian journal of biochemistry Pub Date : 1982-04-01 DOI: 10.1139/o82-049
K C Cho, K C Chow, K K Mark
{"title":"A calorimetric study of the thermal transitions of Halobacterium cutirubrum.","authors":"K C Cho,&nbsp;K C Chow,&nbsp;K K Mark","doi":"10.1139/o82-049","DOIUrl":"https://doi.org/10.1139/o82-049","url":null,"abstract":"<p><p>The thermal transitions of Halobacterium cutirubrum have been examined by differential scanning calorimetry. Two distinct peaks corresponding to the denaturation of two major protein components were observed in the heating curves. One of the peaks has been assigned to the denaturation of the envelope glycoprotein. The variations of the denaturation temperatures with the addition of glucose, glycerol, NaNO3, and NaSCN are consistent with the previous proposal that hydrophobic interactions are essential in stabilizing the glycoprotein.</p>","PeriodicalId":9508,"journal":{"name":"Canadian journal of biochemistry","volume":" ","pages":"419-21"},"PeriodicalIF":0.0,"publicationDate":"1982-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1139/o82-049","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40718569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Comparison of purine and pyrimidine metabolism in G1 and S phases of HeLa and Chinese hamster ovary cells. HeLa和中国仓鼠卵巢细胞G1期和S期嘌呤和嘧啶代谢的比较。
Canadian journal of biochemistry Pub Date : 1982-04-01 DOI: 10.1139/o82-050
J Hordern, J F Henderson
{"title":"Comparison of purine and pyrimidine metabolism in G1 and S phases of HeLa and Chinese hamster ovary cells.","authors":"J Hordern,&nbsp;J F Henderson","doi":"10.1139/o82-050","DOIUrl":"https://doi.org/10.1139/o82-050","url":null,"abstract":"<p><p>Detailed quantitative studies of purine and pyrimidine metabolism in G1 and S phases of synchronized HeLa and Chinese hamster ovary cells have been carried out. Concentrations of ribonucleoside triphosphates increased approximately in proportion to the increase in cell size as cells moved from G1 to S phase. Deoxyribonucleoside triphosphate concentrations were low in G1 phase and increased 2.5- to 10-fold in S phase. Pathways and rates of metabolism of radioactive adenine, guanosine, deoxyadenosine, deoxyguanosine, uridine, cytidine, deoxyuridine, deoxycytidine, and thymidine were determined by measuring the incorporation of each precursor into individual acid-soluble nucleotides and RNA and DNA bases. Cell-cycle or size-dependent differences were detected in many of the parameters studied.</p>","PeriodicalId":9508,"journal":{"name":"Canadian journal of biochemistry","volume":" ","pages":"422-33"},"PeriodicalIF":0.0,"publicationDate":"1982-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1139/o82-050","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40718570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 22
DNA organization in nucleosomes. 核小体中的DNA组织。
Canadian journal of biochemistry Pub Date : 1982-03-01 DOI: 10.1139/o82-043
D P Bazett-Jones, F P Ottensmeyer
{"title":"DNA organization in nucleosomes.","authors":"D P Bazett-Jones,&nbsp;F P Ottensmeyer","doi":"10.1139/o82-043","DOIUrl":"https://doi.org/10.1139/o82-043","url":null,"abstract":"<p><p>A new technique known as electron spectroscopic imaging has allowed the direct visualization of DNA within the nucleosomes of chromatin. The results presented here confirm the model which suggests that approximately two supercoil turns of DNA are wound about the nucleosome core. The structure of nucleosomes from putative transcriptionally active genes, fractionated by preferential sensitivity to DNAase II and solubility in 2 mM MgCl2, has been examined using both dark field electron microscopy and electron spectroscopic imaging. Oligomeric strands of nucleosomes in this fraction have a less distinct beaded appearance than those of bulk chromatin. The phosphorus distribution in this chromatin suggests that the DNA has a less recognizable organization, lacking a two-turn supercoil per subunit. The unique appearance of this fraction in 30 mM NaCl is reversibly changed to the classical beaded appearance when dialyzed into 0.4 M NaCl.</p>","PeriodicalId":9508,"journal":{"name":"Canadian journal of biochemistry","volume":"60 3","pages":"364-70"},"PeriodicalIF":0.0,"publicationDate":"1982-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1139/o82-043","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17345052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
首页 上一页 下一页 尾页
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整，为了账户安全，请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们：info@booksci.cn Book学术提供免费学术资源搜索服务，方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号：481959085
Book学术官方微信