Canadian journal of biochemistry最新文献

Analysis of the expression of cloned genes using an Escherichia coli cell-free system. 利用无细胞大肠杆菌系统分析克隆基因的表达。

Canadian journal of biochemistry Pub Date : 1982-12-01 DOI: 10.1139/o82-140

Y P See, B R Glick

引用次数: 6

Large-scale production of citrate synthase from a cloned gene. 利用克隆基因大规模生产柠檬酸合成酶。

Canadian journal of biochemistry Pub Date : 1982-12-01 DOI: 10.1139/o82-146

H W Duckworth, A W Bell

引用次数: 15

Phosphorylated and unphosphorylated forms of cardiac tropomyosin. 磷酸化和非磷酸化形式的心肌原肌球蛋白。

Canadian journal of biochemistry Pub Date : 1982-12-01 DOI: 10.1139/o82-143

M Segura, E Palmer, J L Saborío

引用次数: 2

Hyper(ADP-ribosyl)ation of histone H1. 组蛋白H1的超(adp -核糖基)化。

Canadian journal of biochemistry Pub Date : 1982-12-01 DOI: 10.1139/o82-139

R J Aubin, V T Dam, J Miclette, Y Brousseau, A Huletsky, G G Poirier

{"title":"Hyper(ADP-ribosyl)ation of histone H1.","authors":"R J Aubin, V T Dam, J Miclette, Y Brousseau, A Huletsky, G G Poirier","doi":"10.1139/o82-139","DOIUrl":"https://doi.org/10.1139/o82-139","url":null,"abstract":"Nucleosomal chains of various repeat unit lengths were generated by a mild micrococcal nuclease digestion of purified pancreatic nuclei. Maximal nucleosome associated poly(ADP-ribose) polymerase activity was recovered in trimeric to tetrameric chromatin fragments, after which the enzyme activity gradually decreased and stabilized towards oligomeric periodicities of 11 to 16 nucleosomes. Electrophoresis of [32P]ADP-ribosylated histones on first-dimension acid-urea or acid-urea-Triton gels and on second-dimension acid--urea--cetyltriammonium bromide gels revealed that, of all histones, only histone H1 could be significantly poly(ADP-ribosyl)ated while only minimal modification could be recovered with histone H1(0). Furthermore, the extent of ADP-ribosylation present on pancreatic histone H1 is shown to proportionally retard this protein's electrophoretic mobility in all gel systems and to consist of a distinct series of at least 12 modification intermediates which can be evidenced, in nuclei or nucleosomes, and fully recovered along with histone H1 upon its selective extraction with 5% perchloric acid. The generation of these increasingly ADP-ribosylated forms of histone H1 is also demonstrated to be time dependent and the more complex ADP-ribosylated forms of this histone are favored at high NAD+ concentrations. Moreover, the electrophoretic mobilities of all intermediates are unaffected by the presence of the nonionic detergent Triton X-100.","PeriodicalId":9508,"journal":{"name":"Canadian journal of biochemistry","volume":"60 12","pages":"1085-94"},"PeriodicalIF":0.0,"publicationDate":"1982-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1139/o82-139","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17361615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

Structure of the glycopeptides of a human gamma 1-immunoglobulin G (Tem) myeloma protein as determined by 360-megahertz nuclear magnetic resonance spectroscopy. 人γ - 1免疫球蛋白G (Tem)骨髓瘤蛋白的糖肽结构，由360兆赫核磁共振波谱测定。

Canadian journal of biochemistry Pub Date : 1982-12-01 DOI: 10.1139/o82-144

A A Grey, S Narasimhan, J R Brisson, H Schachter, J P Carver

引用次数: 34

Purification and characterization of dihydrofolate reductase from Walker 256 carcinosarcoma. Walker 256癌肉瘤中二氢叶酸还原酶的纯化及特性研究。

Canadian journal of biochemistry Pub Date : 1982-12-01 DOI: 10.1139/o82-145

S J Johnson, S V Gupta, K J Stevenson, J H Freisheim

{"title":"Purification and characterization of dihydrofolate reductase from Walker 256 carcinosarcoma.","authors":"S J Johnson, S V Gupta, K J Stevenson, J H Freisheim","doi":"10.1139/o82-145","DOIUrl":"https://doi.org/10.1139/o82-145","url":null,"abstract":"Dihydrofolate reductase of Walker 256 carcinosarcoma has been purified to homogeneity by affinity chromatography. The enzyme reduced 28 mumol dihydrofolate (FAH2) . min-1 . mg protein-1 at 22 degrees C and pH 7.3. Km values with respect to FAH2 and NADPH were 21 and 29 mM, respectively. The IC50 (amount of inhibitor required for 50% loss of enzyme activity) values were 0.2 nM for MTX and aminopterin and 50 and 67 nM, respectively, for N10-formyl FA and triazinate (NSC-139105). The pH maximum is around pH 7.0 and the isoelectric point is 6.8. This reductase has an apparent molecular weight of 21 500. The N-terminal amino acid is valine and the comparison of the N-terminal 20 residues of this reductase shows very high sequence homology with other mammalian reductases. The enzyme contains two cysteine residues and one of these residues is not involved in catalysis. This reductase has four tryptophan residues and modification of one of these residues leads to loss of activity. The intrinsic circular dichroic (CD) spectrum of this reductase is very different from the CD spectra of reductase of Escherichia coli B and L1210/MTX. However, the CD spectra of the enzyme--substrate and enzyme--inhibitor complexes are very similar to that of the L1210/MTX enzyme. This suggests that the ligands may be constrained in similar conformation on the two enzymes. The fluorescence emission maximum at 314 nM when activated at 286 nM is considerably lower than other mammalian enzymes.","PeriodicalId":9508,"journal":{"name":"Canadian journal of biochemistry","volume":"60 12","pages":"1132-42"},"PeriodicalIF":0.0,"publicationDate":"1982-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1139/o82-145","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18180898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Glycerol-induced adenine nucleotide catabolism in rat liver cells. 甘油诱导大鼠肝细胞腺嘌呤核苷酸分解代谢。

Canadian journal of biochemistry Pub Date : 1982-12-01 DOI: 10.1139/o82-141

C Des Rosiers, M Lalanne, J Willemot

引用次数: 1

Effects of glycerol and fructose on purine synthesis de novo and on PP-ribose-P availability in rat liver cells. 甘油和果糖对大鼠肝细胞嘌呤新生合成和pp -核糖- p可利用性的影响。

Canadian journal of biochemistry Pub Date : 1982-12-01 DOI: 10.1139/o82-142

C Des Rosiers, M Lalanne, J Willemot

引用次数: 3

Cytochrome c554 as a possible electron donor in the hydroxylation of ammonia and carbon monoxide in Nitrosomonas europaea. 细胞色素c554在欧洲亚硝化单胞菌氨和一氧化碳羟基化反应中可能的电子供体。

Canadian journal of biochemistry Pub Date : 1982-11-01 DOI: 10.1139/o82-131

D C Tsang, I Suzuki

引用次数: 51

Soluble neutral maltase--glucoamylase from the small intestine: separation and characterization of components with differing affinity for concanavalin A. 来自小肠的可溶性中性麦芽糖酶-葡萄糖淀粉酶:对豆豆蛋白A具有不同亲和力的组分的分离和表征。

Canadian journal of biochemistry Pub Date : 1982-11-01 DOI: 10.1139/o82-129

G Forstner, A Salvatore, L Lee, J Forstner

{"title":"Soluble neutral maltase--glucoamylase from the small intestine: separation and characterization of components with differing affinity for concanavalin A.","authors":"G Forstner, A Salvatore, L Lee, J Forstner","doi":"10.1139/o82-129","DOIUrl":"https://doi.org/10.1139/o82-129","url":null,"abstract":"Intestinal maltase with a neutral pH optimum exists in both a brush border membrane-bound form and a soluble form in suckling rat intestine. Previous experiments in our laboratory have shown that the soluble enzyme contains a component which binds much more tightly to concanavalin A (ConA) than solubilized forms of the membrane enzyme. We studied the origin of this component by subjecting neutral, soluble maltase activity to chromatography on Sepharose 4B at age 13, 18 (preweaning), and 25 (postweaning) days. At 13 days, two maltase peaks were obtained with approximate molecular weights of 400 000 (peak I) and 150 000 (peak II). Peak II was less prominent at 18 days and was absent at 25 days. At 13 days, the majority of peak I consisted of material which was bound between 0.025 and 0.05 M alpha-methyl mannoside on gradient elution chromatography of ConA-Sepharose. Peak II contained material which eluted between 0.075 and 0.3 M alpha-methyl mannoside. At 25 days, all of the soluble maltase eluted between 0.025 and 0.04 M alpha-methyl mannoside. Peak I and peak II maltases had similar pH optima and Km's for maltase. Peak II maltase had a fourfold greater activity toward glycogen than peak I maltase with approximately the same activity for palatinose, turanose, and trehalose. Both maltases were precipitated by an antibody raised against adult membrane-bound maltase. Soluble maltase with neutral pH activity in the suckling rat intestine, therefore, consists of two immunologically related isozymes which differ in their molecular weight, their binding by ConA, and their specificity for glycogen. The small isozyme disappears at or about the time of weaning.","PeriodicalId":9508,"journal":{"name":"Canadian journal of biochemistry","volume":"60 11","pages":"1007-13"},"PeriodicalIF":0.0,"publicationDate":"1982-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1139/o82-129","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17868735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2