Bulletin of The Veterinary Institute in Pulawy最新文献_第5页

Boar sperm quality in relation to presence of sp32-like protein in spermatozoa - preliminary studies 猪精子质量与精子中sp32样蛋白存在的关系——初步研究

Bulletin of The Veterinary Institute in Pulawy Pub Date : 2015-06-01 DOI: 10.1515/BVIP-2015-0041

A. Orzołek, P. Wysocki, J. Strzeżek, M. Koziorowska-Gilun, A. Dziekońska, W. Kordan

{"title":"Boar sperm quality in relation to presence of sp32-like protein in spermatozoa - preliminary studies","authors":"A. Orzołek, P. Wysocki, J. Strzeżek, M. Koziorowska-Gilun, A. Dziekońska, W. Kordan","doi":"10.1515/BVIP-2015-0041","DOIUrl":"https://doi.org/10.1515/BVIP-2015-0041","url":null,"abstract":"Abstract The aim of the study was to analyse sperm proteomes of ejaculates from Polish Large White (PLW) and Polish Landrace (PL) boars and to identify differences which putatively influence semen quality. Spermatozoa protein profiles were analysed by electrophoretic methods followed by selected techniques to evaluate semen quality on the following factors: sperm motility, lipid peroxidation levels (MDA production), ATP content, activities of superoxide dismutase (SOD) and catalase (CAT), total antioxidant status (TAS), and total oxidant status (TOS) of seminal plasma. A protein with an estimated molecular weight of 30 kDa was found in spermatozoa of selected ejaculates. Mass spectrometry demonstrated that this polypeptide is most similar to proacrosin binding protein (sp32). The presence of the protein was more frequently observed in sperm extracts obtained in spring-summer period. Ejaculates containing sp32-like protein demonstrated significantly higher spermatozoa motility, lower inhibition of MDA production by seminal plasma, and higher SOD activity in seminal plasma. Boar semen which included sp32-like protein also demonstrated lower ATP levels in spermatozoa as well as higher TAS and lower TOS of seminal plasma, though the differences were not statistically significant. Ejaculates from PLW boars, with sp32-like protein present in sperm, were characterised by significantly higher sperm motility, lower ATP content in spermatozoa, and higher TAS of seminal plasma. The diminished parameters of semen quality were observed in ejaculates from PL boars that also contained the discussed protein, but the differences were not statistically significant. These findings suggest that the presence of sp32-like protein in boar spermatozoa could influence semen quality","PeriodicalId":9462,"journal":{"name":"Bulletin of The Veterinary Institute in Pulawy","volume":"59 1","pages":"279 - 286"},"PeriodicalIF":0.0,"publicationDate":"2015-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67313882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Antimicrobial resistance of Enterococcus faecalis isolated from meat 从肉类中分离的粪肠球菌的抗微生物药物耐药性

Bulletin of The Veterinary Institute in Pulawy Pub Date : 2015-06-01 DOI: 10.1515/BVIP-2015-0034

H. Różańska, Aleksandra Lewtak-Piłat, J. Osek

引用次数: 14

Effects of flunixin and florfenicol combined with vitamins E and/or C on selected immune mechanisms in cattle under conditions of adaptive stress 氟尼辛和氟苯尼考与维生素E和/或C联合使用对适应性应激条件下牛特定免疫机制的影响

Bulletin of The Veterinary Institute in Pulawy Pub Date : 2015-06-01 DOI: 10.1515/BVIP-2015-0043

R. Urban-Chmiel, Rafał Stachura, P. Hola, A. Puchalski, M. Dec, A. Wernicki

{"title":"Effects of flunixin and florfenicol combined with vitamins E and/or C on selected immune mechanisms in cattle under conditions of adaptive stress","authors":"R. Urban-Chmiel, Rafał Stachura, P. Hola, A. Puchalski, M. Dec, A. Wernicki","doi":"10.1515/BVIP-2015-0043","DOIUrl":"https://doi.org/10.1515/BVIP-2015-0043","url":null,"abstract":"Abstract The aim of the study was to evaluate the effect of flunixin and florfenicol administered in combination with vitamin E or C on selected leukocyte immune mechanisms and on the inflammatory process during the first few weeks in the feedlot. Fifty calves divided into 5 groups (n = 10) received florfenicol and flunixin with vitamin E or C. Blood was collected on the 1st, 3rd, 7th, 14th, 21st, and 28th d of the experiment. Intracellular metabolism (NBT), apoptosis, chemotaxis, susceptibility to M. haemolytica leukotoxin, and expression of β2-integrins were determined in leukocytes. The symptoms of respiratory tract infection were observed in 40% of calves in control group, while in the other groups the morbidity rate ranged from 10% to 20%. Leukocytes showed decreased NBT, and the mean values for apoptosis ranged from 14% to 24%. The lowest percentage of apoptotic cells was observed in the calves that received florfenicol with flunixin and vitamins E and C. The chemotactic activity confirmed the significant inhibitory effect of the preparations on migration of the cells. A significant decrease (P ≤ 0.05) in the susceptibility of leukocytes to leukotoxin was noted in the group that received florfenicol and flunixin with vitamin E. Expression of β2-integrin receptors was the lowest in calves receiving florfenicol with flunixin and vitamin E or C. The application of an antibiotic and a non-steroidal anti-inflammatory drug with antioxidants protected the leukocytes involved in defence against M. haemolytica virulence factors and effectively limited oxidative stress in the calves.","PeriodicalId":9462,"journal":{"name":"Bulletin of The Veterinary Institute in Pulawy","volume":"59 1","pages":"295 - 301"},"PeriodicalIF":0.0,"publicationDate":"2015-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/BVIP-2015-0043","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67313708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Sequence analysis of minor protein genes of equine arteritis virus during persistent infection 马动脉炎病毒持续感染期间次要蛋白基因序列分析

Bulletin of The Veterinary Institute in Pulawy Pub Date : 2015-06-01 DOI: 10.1515/BVIP-2015-0027

J. Rola, W. Socha, J. Żmudziński

{"title":"Sequence analysis of minor protein genes of equine arteritis virus during persistent infection","authors":"J. Rola, W. Socha, J. Żmudziński","doi":"10.1515/BVIP-2015-0027","DOIUrl":"https://doi.org/10.1515/BVIP-2015-0027","url":null,"abstract":"Abstract The variability of the ORF2a, ORF2b, ORF3, and ORF4 genes of the equine arteritis virus (EAV) was analysed during a seven year observation of persistent infection in a stallion of the Malopolska breed. A total of 11 semen samples were collected between 2004 and 2011. RNA of EAV isolates obtained from the semen of the stallion was amplified, sequenced, and compared with the sequences of other strains available in GenBank. Multiple nucleotide substitutions were found in sequences of the analysed regions, however, neither deletion nor insertions were detected. The highest number of point mutations (11-6 synonymous and 5 non-synonymous) were found in the ORF2b gene, and the lowest number of substitutions (6-5 synonymous and one non-synonymous) were found in the ORF2a gene. None of the identified mutations affected any of the glycosylation or phosphorylation sites of the minor EAV protein. Phylogenetic analysis of the ORF3 gene of EAV isolates showed that they grouped together within the cluster of European strains of EAV. Additionally, the ORF3 gene sequences of the isolates showed high (86.4% - 98.3%) similarity to the previously isolated Polish EAV strains.","PeriodicalId":9462,"journal":{"name":"Bulletin of The Veterinary Institute in Pulawy","volume":"59 1","pages":"179 - 184"},"PeriodicalIF":0.0,"publicationDate":"2015-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67311082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Morphometric dimensions of the stallion sperm head depending on the staining method used 种马精子头的形态测量尺寸取决于所使用的染色方法

Bulletin of The Veterinary Institute in Pulawy Pub Date : 2015-06-01 DOI: 10.1515/BVIP-2015-0039

D. Banaszewska, Magdalena Czubaszek, R. Walczak-Jędrzejowska, K. Andraszek

{"title":"Morphometric dimensions of the stallion sperm head depending on the staining method used","authors":"D. Banaszewska, Magdalena Czubaszek, R. Walczak-Jędrzejowska, K. Andraszek","doi":"10.1515/BVIP-2015-0039","DOIUrl":"https://doi.org/10.1515/BVIP-2015-0039","url":null,"abstract":"Abstract Semen was collected from Polish Halfbred stallions. Twenty individuals from 3 to 4 years of age were selected for the study. At least one ejaculate from each stallion was collected and assessed. Sperm morphology was evaluated using Papanicolaou stain, SpermBlue®, and a complex of eosin and gentian stain, whereas unstained slides were used as control samples. Morphometric measurements were performed on 100 randomly selected sperm heads in each ejaculate. The length, width, perimeter, and surface area of the sperm head were measured. The frequency of nuclear vacuoles was determined as well. Tygerberg’s strict criteria, which most precisely characterise the sperm head, were used in the morphological evaluation of the sperm. The results obtained indicate that in the case of staining with SpermBlue® and with eosin+gentian complex, the morphometry of the sperm head seems to be most similar to that observed in unstained smears. It also seems that neither shrinking nor swelling is uniform in the various staining techniques (Papanicolaou stain). Moreover, it appears that in comparison with unstained sperm, all methods caused the width of the head to increase as a result of swelling.","PeriodicalId":9462,"journal":{"name":"Bulletin of The Veterinary Institute in Pulawy","volume":"30 1","pages":"263 - 270"},"PeriodicalIF":0.0,"publicationDate":"2015-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/BVIP-2015-0039","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67313126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Polymorphism of the PrP prion protein gene in Polish Merino and old-type Polish Merino from Brylewo flock 波兰美利奴羊和老式波兰美利奴羊PrP朊蛋白基因多态性分析

Bulletin of The Veterinary Institute in Pulawy Pub Date : 2015-06-01 DOI: 10.1515/BVIP-2015-0047

R. Niżnikowski, A. Oprządek, G. Czub, M. Świątek, Dariusz Kubasik, Paweł Brzoza, K. Głowacz, M. Ślęzak

{"title":"Polymorphism of the PrP prion protein gene in Polish Merino and old-type Polish Merino from Brylewo flock","authors":"R. Niżnikowski, A. Oprządek, G. Czub, M. Świątek, Dariusz Kubasik, Paweł Brzoza, K. Głowacz, M. Ślęzak","doi":"10.1515/BVIP-2015-0047","DOIUrl":"https://doi.org/10.1515/BVIP-2015-0047","url":null,"abstract":"Abstract The study was conducted on Polish Merino (143♀ and 39♂) and old-type Polish Merino sheep (176♀ and 61♂) in Brylewo flock (Wielkopolskie Province). The examined sheep were at the age of one year. Prion protein (PrP) genotype was determined in all animals. In both breeds four alleles (ARR, ARQ, AHQ, VRQ) were found. Highly significant effect of breed and insignificant impact of gender within breed was observed in regard to the frequency of occurrence of alleles and genotypes susceptible to classical scrapie in the sheep. Eight different PrP genotypes in Polish Merino and nine genotypes in old-type Polish Merino were identified. Very high frequency of ARR/ARR genotype in old-type Polish Merino and high frequency of ARR/ARQ genotype in Polish Merino were found, with relatively significant frequency of occurrence of the genotypes containing VRQ allele. In old-type Polish Merino, three animals (of both genders) had VRQ/VRQ genotype. Breeding work involving elimination of animals encoding valine at codon 136, and introduction of rams with ARR allele to the population increased the frequency of occurrence of ARR/ARR genotype and ARR allele in the population of old-type Polish Merino. To improve the distribution of the genotypes genetically resistant to scrapie in the flock of Polish Merino only rams with ARR/ARR genotype were left. This guarantees an increase in the frequency of occurrence of genotypes genetically resistant to scrapie in the offspring.","PeriodicalId":9462,"journal":{"name":"Bulletin of The Veterinary Institute in Pulawy","volume":"59 1","pages":"321 - 326"},"PeriodicalIF":0.0,"publicationDate":"2015-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/BVIP-2015-0047","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67313665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunohistochemical detection of tumour cell proliferation and intratumoural microvessel density in canine malignant mammary tumours 犬乳腺恶性肿瘤肿瘤细胞增殖及瘤内微血管密度的免疫组化检测

Bulletin of The Veterinary Institute in Pulawy Pub Date : 2015-06-01 DOI: 10.1515/BVIP-2015-0038

G. Sennazli, F. Yıldırım, Seckin Serdar Arun, A. Gurel, K. Sonmez

{"title":"Immunohistochemical detection of tumour cell proliferation and intratumoural microvessel density in canine malignant mammary tumours","authors":"G. Sennazli, F. Yıldırım, Seckin Serdar Arun, A. Gurel, K. Sonmez","doi":"10.1515/BVIP-2015-0038","DOIUrl":"https://doi.org/10.1515/BVIP-2015-0038","url":null,"abstract":"Abstract The objective of this study was to investigate the correlation between different histological types and grades of canine malignant mammary tumours, tumour cell proliferation and their angiogenic activity using immunohistochemical markers. Mammary tissue samples from 47 bitches with mammary cancer were evaluated. The expression of cellular proliferation marker Ki-67 and endothelial marker Von Willebrand’s factor (vWF) were immunohistochemically demonstrated. The tumours with the highest Ki-67 and vWF expressions were found to share similar histomorphological features. Simple solid carcinoma had the highest levels of Ki-67, vWF, and higher histological grade while complex carcinomas, osteosarcomas, and carcinosarcomas had the lowest ones. The differences between the expressions of Ki-67 and vWF in different tumour types were considered to be of great importance in determination of biological behaviour and prognosis of these tumours. This study is one of the few studies that evaluate these differences among the subtypes of malignant canine mammary tumours","PeriodicalId":9462,"journal":{"name":"Bulletin of The Veterinary Institute in Pulawy","volume":"163 1","pages":"255 - 261"},"PeriodicalIF":0.0,"publicationDate":"2015-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/BVIP-2015-0038","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67313245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Evaluation of the proliferative activity of immunocompetent cells in the jejunal and iliac lymph nodes of prepubertal female wild boars diagnosed with mixed mycotoxicosis 诊断为混合性真菌中毒的青春期前雌性野猪空肠和髂淋巴结免疫活性细胞增殖活性的评价

Bulletin of The Veterinary Institute in Pulawy Pub Date : 2015-06-01 DOI: 10.1515/BVIP-2015-0030

Ł. Zielonka, E. Jakimiuk, K. Obremski, M. Gajęcka, M. Da̧browski, M. Gajęcki

引用次数: 7

Identification of strains with phenotypes similar to those of Staphylococcus aureus isolated from table chicken eggs using MALDI-TOF MS and genotyping methods 用MALDI-TOF质谱和基因分型方法鉴定与食用鸡蛋中金黄色葡萄球菌表型相似的菌株

Bulletin of The Veterinary Institute in Pulawy Pub Date : 2015-06-01 DOI: 10.1515/BVIP-2015-0035

A. Marek, E. Pyzik, D. Stępień-Pyśniak, T. Hauschild, T. Banach

{"title":"Identification of strains with phenotypes similar to those of Staphylococcus aureus isolated from table chicken eggs using MALDI-TOF MS and genotyping methods","authors":"A. Marek, E. Pyzik, D. Stępień-Pyśniak, T. Hauschild, T. Banach","doi":"10.1515/BVIP-2015-0035","DOIUrl":"https://doi.org/10.1515/BVIP-2015-0035","url":null,"abstract":"Abstract The aim of the study was to identify the affinity of 10 Staphylococcus strains isolated from table chicken eggs to specific species. Preliminary analysis performed by API ID32 Staph test identified these strains as S. aureus, but they exhibited a negative reaction in the tube coagulase test. Thus, the analysed strains were initially characterised as Staphylococcus aureus-like (SAL). Further characterisation was performed by genotypic methods, using restriction fragment length polymorphism (RFLP) of the coagulase gene (coa) and sequencing of the gene rpoB. An attempt was also made to identify the isolated Staphylococcus strains by MALDI-TOF mass spectrometry. The results indicated that none of the strains tested belonged to the species S. aureus. The rpoB sequences of five isolates showed the highest sequence similarity to S. haemolyticus, three isolates to S. chromogenes, and one isolate to S. epidermidis. One strain (SAL4) remained unidentified in this analysis. The results obtained using mass spectrometry were comparable to those based on gene sequence analysis. Strain SAL4, which could not be identified by sequencing, was identified by MALDI-TOF as Staphylococcus chromogenes.","PeriodicalId":9462,"journal":{"name":"Bulletin of The Veterinary Institute in Pulawy","volume":"59 1","pages":"235 - 239"},"PeriodicalIF":0.0,"publicationDate":"2015-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/BVIP-2015-0035","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67312266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Immunohistochemical evaluation of neoangiogenesis in canine mast cell tumours 犬肥大细胞肿瘤新生血管生成的免疫组化评价

Bulletin of The Veterinary Institute in Pulawy Pub Date : 2015-06-01 DOI: 10.1515/BVIP-2015-0037

M. Kandefer-Gola, J. Madej, S. Dzimira, I. Janus, M. Nowak, R. Ciaputa

引用次数: 2