Bulletin of The Veterinary Institute in Pulawy最新文献_第4页

Optimisation of protocol for Clostridium botulinum detection in mink feed 水貂饲料中肉毒梭菌检测方案的优化

Bulletin of The Veterinary Institute in Pulawy Pub Date : 2015-09-01 DOI: 10.1515/BVIP-2015-0056

T. Grenda, E. Kukier, M. Goldsztejn, K. Kwiatek, N. Kozieł

引用次数: 1

Effects of Sidr honey on second-intention healing of contaminated full-thickness skin wounds in healthy dogs 锡德蜂蜜对健康犬污染全层皮肤创面二次愈合的影响

Bulletin of The Veterinary Institute in Pulawy Pub Date : 2015-09-01 DOI: 10.1515/BVIP-2015-0063

W. Hananeh, Z. Ismail, M. Alshehabat, M. A. Abeeleh, J. H. Ali

{"title":"Effects of Sidr honey on second-intention healing of contaminated full-thickness skin wounds in healthy dogs","authors":"W. Hananeh, Z. Ismail, M. Alshehabat, M. A. Abeeleh, J. H. Ali","doi":"10.1515/BVIP-2015-0063","DOIUrl":"https://doi.org/10.1515/BVIP-2015-0063","url":null,"abstract":"Abstract The therapeutic effects of Sidr honey on second-intention healing of contaminated full-thickness skin wounds in dogs were investigated. Povidone-iodine was used as a standard treatment and served as a control. Healthy adult (two-to-four-year-old) mongrels, comprising six dogs and two bitches, were divided into four equal groups in order to obtain multi-aged wounds at the end of the study. Four 2cm × 2cm full-thickness skin wounds were created on both sides of the back area of each dog under general anaesthesia adhering to aseptic technique. Contaminated wounds were then divided into two treatment groups: Group 1, Sidr honey treated (right side wounds) and group 2, povidone-iodine treated (left side wounds). All wounds were evaluated grossly daily at the time of treatment application and digitally photographed once every week. Images were analysed using ImageJ software. The parameters of unhealed wound area and length of advancing epithelium were obtained. The epithelialisation areas, percentage of wound area, and wound contraction rate were then calculated. No significant differences were found between the two treatment groups in any of the parameters studied. Overall, both honey treated and iodine treated wounds healed well within the time period of the study (28 d). However, the study showed a beneficial effect of Sidr honey on second-intention healing of full thickness contaminated wounds in healthy dogs and the effect was comparable to that of Povidone iodine.","PeriodicalId":9462,"journal":{"name":"Bulletin of The Veterinary Institute in Pulawy","volume":"59 1","pages":"433 - 439"},"PeriodicalIF":0.0,"publicationDate":"2015-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/BVIP-2015-0063","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67314610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Review of animal models used to study effects of bee products on wound healing: findings and applications 用于研究蜂产品对伤口愈合影响的动物模型综述:发现和应用

Bulletin of The Veterinary Institute in Pulawy Pub Date : 2015-09-01 DOI: 10.1515/BVIP-2015-0062

W. Hananeh, Z. Ismail, M. Alshehabat, Jaafar A. Ali

{"title":"Review of animal models used to study effects of bee products on wound healing: findings and applications","authors":"W. Hananeh, Z. Ismail, M. Alshehabat, Jaafar A. Ali","doi":"10.1515/BVIP-2015-0062","DOIUrl":"https://doi.org/10.1515/BVIP-2015-0062","url":null,"abstract":"Abstract Non-healing wounds are associated with high morbidity and might greatly impact a patient’s well-being and economic status. For many years, scientific research has focused on developing and testing several natural and synthetic materials that enhance the rate of wound healing or eliminate healing complications. Honey has been used for thousands of years as a traditional remedy for many ailments. Recently, honey has reemerged as a promising wound care product especially for infected wounds and for wounds in diabetic patients. In addition to its proposed potent broad-spectrum antibacterial properties, honey has been claimed to promote wound healing by reducing wound hyperaemia, oedema, and exudate, and by stimulating angiogenesis, granulation tissue formation and epithelialisation. Several animal models, including large animals, dogs and cats, and different species of laboratory animals have been used to investigate the efficacy and safety of various natural and synthetic agents for wound healing enhancement. Interpreting the results obtained by these studies is, however, rather difficult and usually hampered by many limiting factors including great variation in types and origins of honey, the type of animal species used as models, the type of wounds, the number of animals, the number and type of controls, and variation in treatment protocols. In this article, we provide a comprehensive review of the most recent findings and applications of published experimental and clinical trials using honey as an agent for wound healing enhancement in different animal models.","PeriodicalId":9462,"journal":{"name":"Bulletin of The Veterinary Institute in Pulawy","volume":"59 1","pages":"425 - 431"},"PeriodicalIF":0.0,"publicationDate":"2015-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/BVIP-2015-0062","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67314865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Impact of low-concentrated acidic electrolysed water obtained by membrane electrolysis on the decontamination of meat microbiota 膜电解低浓酸性电解水对肉类微生物群去污的影响

Bulletin of The Veterinary Institute in Pulawy Pub Date : 2015-09-01 DOI: 10.1515/BVIP-2015-0055

Ewa Brychcy, A. Jarmoluk, K. Marycz

{"title":"Impact of low-concentrated acidic electrolysed water obtained by membrane electrolysis on the decontamination of meat microbiota","authors":"Ewa Brychcy, A. Jarmoluk, K. Marycz","doi":"10.1515/BVIP-2015-0055","DOIUrl":"https://doi.org/10.1515/BVIP-2015-0055","url":null,"abstract":"Abstract The influence of acidic electrolysed water (AEW) treatment on inactivation of pure bacterial cultures inoculated onto the surface of agarised media and surface microbiota of pork meat were examined. Low-concentrated AEW (low concentration of sodium chloride and low current electrolysis) was generated by electrolysis (5 or 10 min) of 0.001% or 0.01% NaCl solution. The number of viable microorganisms was determined using a plate count method. The effect of AEW on bacterial cell morphology were investigated using scanning electron microscopy (SEM). After treatment with AEW, a significant, about 3.00 log reduction of Pseudomonas fluorescens, Yersinia enterocolitica, Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Listeria monocytogenes, and Micrococcus luteus populations was observed. In the AEW treatment of pork, the highest reduction of total number of microorganisms (2.1 log reduction), yeast and moulds (2.5-2.6 log reduction), and psychrotrophs (more than 1 log reduction) was observed after spraying with 0.001% NaCl subjected to 10 min electrolysis. SEM revealed disruption and lysis of E. coli and S. aureus cells treated with AEW, suggesting a bactericidal effect. Higher available chlorine concentration (0.37-8.45 mg/L), redox potential (863.1-1049.8 mV), and lower pH (2.73-3.70) had an influence on the shape of bacteria and the number of breaks in the bacterial membrane.","PeriodicalId":9462,"journal":{"name":"Bulletin of The Veterinary Institute in Pulawy","volume":"59 1","pages":"369 - 376"},"PeriodicalIF":0.0,"publicationDate":"2015-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/BVIP-2015-0055","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67314207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Total antioxidative capacity and zinc concentration in dogs suffering from perianal tumours 患肛周肿瘤犬的总抗氧化能力和锌浓度

Bulletin of The Veterinary Institute in Pulawy Pub Date : 2015-09-01 DOI: 10.1515/BVIP-2015-0061

A. Brodzki, P. Brodzki, M. Tatara, K. Kostro

{"title":"Total antioxidative capacity and zinc concentration in dogs suffering from perianal tumours","authors":"A. Brodzki, P. Brodzki, M. Tatara, K. Kostro","doi":"10.1515/BVIP-2015-0061","DOIUrl":"https://doi.org/10.1515/BVIP-2015-0061","url":null,"abstract":"Abstract The aim of the study was to determine total antioxidative capacity (TAC) and zinc concentration in serum of dogs suffering from perianal tumours just before the start of the antihormonal treatment (AHT) and one and six months later. The study was performed on 45 dogs divided into two groups: control group suffering from non-malignant tumours (N = 24) and a group with malignant neoplastic changes (N = 21). Serum TAC and zinc concentrations were measured using photometric and atomic absorption spectrophotometric methods. Six months after the start of the AHT, TAC was significantly lower by 10.6% in dogs with malignant tumours when compared to controls (P = 0.03). In the non-malignant group, serum zinc concentration was higher before the treatment than in the malignant group, while the opposite results were observed six months later (P < 0.001). In the non-malignant group, gradually decreasing values of serum zinc concentration at each stage of the investigation were observed, while the opposite results were obtained in the malignant group (P < 0.05). The obtained results indicate that malignant neoplastic process is associated with significantly reduced TAC. Determination of serum zinc concentration in dogs with non-malignant and malignant perianal tumours may have practical diagnostic and prognostic values and may serve towards increasing the effectiveness of AHT monitoring.","PeriodicalId":9462,"journal":{"name":"Bulletin of The Veterinary Institute in Pulawy","volume":"59 1","pages":"417 - 423"},"PeriodicalIF":0.0,"publicationDate":"2015-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67314714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Impact of the ageing process on the intensity of post mortem proteolysis and tenderness of beef from crossbreeds 老化过程对杂交牛肉死后蛋白水解强度和嫩度的影响

Bulletin of The Veterinary Institute in Pulawy Pub Date : 2015-09-01 DOI: 10.1515/BVIP-2015-0054

M. Moczkowska, A. Półtorak, A. Wierzbicka

{"title":"Impact of the ageing process on the intensity of post mortem proteolysis and tenderness of beef from crossbreeds","authors":"M. Moczkowska, A. Półtorak, A. Wierzbicka","doi":"10.1515/BVIP-2015-0054","DOIUrl":"https://doi.org/10.1515/BVIP-2015-0054","url":null,"abstract":"Abstract The aim of the study was the evaluation of the effect of ageing on the extent of myofibrillar proteins degradation and tenderness of beef in different crossbreeds, BB × HF and SM × HF, from which the musculus semitendinosus was obtained. The pH value, basic composition of meat, and colour parameters were determined on the 3rd d post mortem. The Warner Bratzler shear force and the extent of protein degradation were evaluated in regard to the effect of ageing time. Meat of BB × HF crossbreed had a lower amount of intramuscular fat and higher protein content (P ≤ 0.05). The shear force decreased with ageing time in the case of both crossbreeds. However, the highest values were noted in SM × HF crossbreed on days 3 and 7 of ageing. The differences in proteolysis of myofibrillar proteins and polypeptides, determined by SDS-PAGE electrophoresis, were observed between crossbreeds and the ageing time. A significant decrease in desmin and increased levels of 49-46 kDa and 32-27 kDa polypeptides (products of proteolytic degradation) were observed with an increasing ageing time. In addition, the rate of increase in the amount of 32-27 kDa polypeptides was more significant in BB × HF crossbreed. The data obtained showed that tenderness and the extent of protein degradation are associated with ageing process and animals’ genotype.","PeriodicalId":9462,"journal":{"name":"Bulletin of The Veterinary Institute in Pulawy","volume":"59 1","pages":"361 - 367"},"PeriodicalIF":0.0,"publicationDate":"2015-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/BVIP-2015-0054","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67314165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Selected biochemical and oxidative stress parameters and ceruloplasmin as acute phase protein associated with bovine leukaemia virus infection in dairy cows 选定的生化和氧化应激参数和铜蓝蛋白作为奶牛白血病病毒感染相关的急性期蛋白

Bulletin of The Veterinary Institute in Pulawy Pub Date : 2015-09-01 DOI: 10.1515/BVIP-2015-0048

P. Akalın, V. Ataseven, Dogan Fırat, Y. Ergün, N. Başpınar, O. Özcan

{"title":"Selected biochemical and oxidative stress parameters and ceruloplasmin as acute phase protein associated with bovine leukaemia virus infection in dairy cows","authors":"P. Akalın, V. Ataseven, Dogan Fırat, Y. Ergün, N. Başpınar, O. Özcan","doi":"10.1515/BVIP-2015-0048","DOIUrl":"https://doi.org/10.1515/BVIP-2015-0048","url":null,"abstract":"Abstract The aim of this study was to determine the ceruloplasmin (Cp) and vitamin C concentrations, the total antioxidant status (TAS), and selected biochemical parameters in dairy cows spontaneously infected with bovine leukaemia virus (BLV). Of the 27 cows included in the study, 18 animals were seropositive for enzootic bovine leukosis (EBL), whereas nine cows were seronegative and were used as controls. The serum aspartate aminotransferase (AST) (P = 0.003) and Cp concentrations (P = 0.03) decreased (65.17 ± 5.03 and 7.70 ± 0.72 respectively) in BLV-infected cows, as compared to healthy animals (100.67 ± 11.50 and 10.40 ± 0.70 respectively). A slight insignificant increase in alkaline phosphatase activity and unchanged levels of alanine aminotransferase, lactate dehydrogenase, calcium, magnesium, and TAS were demonstrated in EBL cows. As the TAS and vitamin C levels remained unchanged in EBL cows, it may be suggested that ruminants may compensate for the impaired oxidative/antioxidative balance. The results obtained also indicate that BLV may suppress AST and Cp synthesis or secretion in the liver through an unknown mechanism. The mechanism of action of BLV in hepatocytes, especially on AST and Cp, requires further investigation to elucidate the immune suppression caused by oncogenic retroviruses.","PeriodicalId":9462,"journal":{"name":"Bulletin of The Veterinary Institute in Pulawy","volume":"59 1","pages":"327 - 330"},"PeriodicalIF":0.0,"publicationDate":"2015-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/BVIP-2015-0048","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67314215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Indirect relationship between lipophilicity and maximum residue limit of drugs determined for fatty tissue 脂肪组织中药物亲脂性与最大残留限量的间接关系

Bulletin of The Veterinary Institute in Pulawy Pub Date : 2015-09-01 DOI: 10.1515/BVIP-2015-0057

M. Marczak, K. Okoniewska, J. Okoniewski, T. Grabowski, J. Jaroszewski

{"title":"Indirect relationship between lipophilicity and maximum residue limit of drugs determined for fatty tissue","authors":"M. Marczak, K. Okoniewska, J. Okoniewski, T. Grabowski, J. Jaroszewski","doi":"10.1515/BVIP-2015-0057","DOIUrl":"https://doi.org/10.1515/BVIP-2015-0057","url":null,"abstract":"Abstract The aim of this study was to determine the correlation between lipophilicity and maximum residue limit (MRL) value specified for veterinary drugs in the fatty tissue of various animal species. The analysis was performed on a group of 73 compounds with different modes of action and MRL values determined for the fatty tissue of animals. Additionally, the logarithm of water/organic phase partition ratio (LogP) and the ratio of ionised and unionised substance in buffer with pH 7.4 (LogD7.4) were calculated. The main analysis was performed after the division of the whole group into six fractions. The linear correlation and regression analysis were used to determine the indirect relationship between the mean arithmetic value of LogP or LogD7.4 in selected fractions and related LogMRL of the drugs tested. The calculations revealed a linear correlation between fractioned lipophilicity and LogMRL values for the analysed compounds. The existence of indirect relationship between lipophilicity and MRL values determined for fatty tissue was confirmed.","PeriodicalId":9462,"journal":{"name":"Bulletin of The Veterinary Institute in Pulawy","volume":"205 1","pages":"383 - 391"},"PeriodicalIF":0.0,"publicationDate":"2015-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/BVIP-2015-0057","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67314688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Occurrence and characterisation of MRSA and extended-spectrum ß-lactamases producing Escherichia coli isolated from mastitic cows’ milk 乳牛乳中MRSA和产ß-内酰胺酶大肠埃希菌分离株的发生及特征分析

Bulletin of The Veterinary Institute in Pulawy Pub Date : 2015-06-01 DOI: 10.1515/BVIP-2015-0029

M. Weiner, H. Różańska, Maria Kubajka, K. Szulowski, M. Krajewska, B. Wasiński

{"title":"Occurrence and characterisation of MRSA and extended-spectrum ß-lactamases producing Escherichia coli isolated from mastitic cows’ milk","authors":"M. Weiner, H. Różańska, Maria Kubajka, K. Szulowski, M. Krajewska, B. Wasiński","doi":"10.1515/BVIP-2015-0029","DOIUrl":"https://doi.org/10.1515/BVIP-2015-0029","url":null,"abstract":"Abstract The aim of study was the preliminary evaluation of the occurrence of methicillin-resistant Staphylococcus aureus (MRSA) and extended spectrum ß-lactamases (ESBL) - producing Escherichia coli in 650 milk and inflammatory secretions from cows with clinical or subclinical mastitis. One millilitre of the sample was added to Mueller-Hinton broth supplemented with 6.5% NaCl, Tryptone Soya Broth with cefoxitin and aztreonam, and then to MRSA ID agar. Presumptive MRSA colonies were analysed for the presence of mecA gene. Parallel to MRSA identification, the samples were incubated in buffered peptone water, lauryl tryptose broth and McConkey agar supplemented with cefotaxim for ESBL-producing E. coli isolation. These bacteria were identified using API Rapid 32 E and the ability of ESBL production was initially established using disc test D68C and confirmed by MIC technique using Sensititre ESBL plates. The primers (blaCTX, blaTEM, blaSHV, and blaCMY-2-group) for the detection of some of the genes encoding ESBL production were used. The 45 strains of S. aureus with mecA gene and 41 strains of E. coli with blaTEM gene were detected.","PeriodicalId":9462,"journal":{"name":"Bulletin of The Veterinary Institute in Pulawy","volume":"59 1","pages":"191 - 195"},"PeriodicalIF":0.0,"publicationDate":"2015-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/BVIP-2015-0029","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67311368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Effect of therapeutic doses of enrofloxacin on circulating lymphocyte subpopulations in pigs 恩诺沙星治疗剂量对猪循环淋巴细胞亚群的影响

Bulletin of The Veterinary Institute in Pulawy Pub Date : 2015-06-01 DOI: 10.1515/BVIP-2015-0042

M. Pomorska-Mól, Z. Pejsak

{"title":"Effect of therapeutic doses of enrofloxacin on circulating lymphocyte subpopulations in pigs","authors":"M. Pomorska-Mól, Z. Pejsak","doi":"10.1515/BVIP-2015-0042","DOIUrl":"https://doi.org/10.1515/BVIP-2015-0042","url":null,"abstract":"Abstract Twenty pigs of similar genetics (PIC) were used. Pigs were randomly divided into two groups: experimental (ENRO, n = 10) and control (C, n = 10). From day 0 to day 4, pigs from ENRO group received enrofloxacin at the recommended therapeutic dose. Pigs from C group received PBS instead of enrofloxacin. Blood samples were collected on days 0 (before antibiotic administration), 2, 4 (during antibiotic therapy), 9, and 13 of the study (after enrofloxacin administration). Haematological examination and flow cytometry were used to establish the relative and absolute counts of various leukocyte subsets. Lymphocyte subpopulations were measured by fluorochrom-labelled antibodies according to following definitions: CD3+ (T cells), CD21+ (B cells), CD4+CD8- (helper T cells, Th), CD4-CD8+ (cytolytic T cells, CLT), CD4+CD8+ (cytolytic and memory T cells). The present study revealed the modulating effect of enrofloxacin on the composition of circulating lymphocytes in pigs. Concentration and percentage of CD8+ cells decreased significantly after treatment with enrofloxacin and as a result the absolute CD4/CD8 ratio increased significantly as compared to control group (P < 0.05).These findings should prompt further studies on the practical significance of the results obtained in terms of clinical implications. In view of the results, it cannot be excluded that enrofloxacin may also have immunomodulatory effects on host response to infection.","PeriodicalId":9462,"journal":{"name":"Bulletin of The Veterinary Institute in Pulawy","volume":"59 1","pages":"287 - 293"},"PeriodicalIF":0.0,"publicationDate":"2015-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67313636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4