Anti-inflammatory & anti-allergy agents in medicinal chemistry最新文献

{"title":"The Structural Analogy of MASP-2 with Thrombin and MASP-1 Reveals Strong Binding with PAR4.","authors":"Uzma Saqib, Mridul Madhuri, Sumati Hajela, Sadhana Sharma, Krishnan Hajela","doi":"10.2174/0118715230411505251126063432","DOIUrl":"10.2174/0118715230411505251126063432","url":null,"abstract":"<p><strong>Introduction: </strong>The historical discovery that thrombin activates Protease-Activated Receptor 4 (PAR4) has paved the way for several novel findings. Besides thrombin, the complement lectin pathway protease Mannose-Binding Lectin-Associated Serine Protease-1 (MASP-1) also binds to PAR4, albeit with lower affinity. Similar to thrombin, MASP-1 activates Ca². signaling pathways in endothelial cells. MASP-2, a homolog of MASP-1, plays an important role in complement activation; however, its direct interaction with PAR4 has not yet been elucidated. In this study, we performed structural investigations of thrombin, MASP-1, and MASP-2 to evaluate their binding affinities toward the PAR4 peptide.</p><p><strong>Methods: </strong>We employed in silico docking, binding affinity calculations, molecular dynamics simulations, and mutagenesis studies to test our hypothesis.</p><p><strong>Results: </strong>For the first time, we demonstrate that, like thrombin and MASP-1, MASP-2 binds to PAR4 with appreciable affinity and could serve as a potential agonist of the PAR4 receptor and its associated inflammatory signaling pathways.</p><p><strong>Discussion: </strong>The high sequence similarity of MASP-2 with MASP-1 and thrombin is an important factor in attaining comparable binding with the PAR4 peptide.</p><p><strong>Conclusion: </strong>Our findings may pave the way for future investigations into the signaling mechanisms and therapeutic potential of PAR4-mediated inflammatory diseases.</p>","PeriodicalId":94368,"journal":{"name":"Anti-inflammatory & anti-allergy agents in medicinal chemistry","volume":" ","pages":"129-142"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146055681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and Evaluation of Antifungal Activity of Carbopol and Neem Seed Oil-Based Organogels.","authors":"Piyush Jain, Shashikant Chandrakar, Pushpa Prasad Gupta, Bina Gidwani","doi":"10.2174/0118715230381640250905045919","DOIUrl":"10.2174/0118715230381640250905045919","url":null,"abstract":"<p><strong>Introduction: </strong>The present study focused on the formulation of a Sertaconazole Nitrate (SN) organogel with neem seed oil through microwave irradiation, optimizing the formulation by Box-Behnken Design (BBD) and Response Surface Methodology (RSM). The research explored the effect of Neem Seed Oil (NSO), Carbopol-934 (CP), and Polyethylene glycol-400 (PEG) on viscosity (VS), spreadability (SP), and drug content (DC).</p><p><strong>Methods: </strong>A 15-run BBD was utilized to investigate the impact of the independent variables. The organogels obtained were analyzed for viscosity, spreadability, and drug content. The optimized formulation was also characterized for homogeneity, pH, swelling index, extrudability, drugexcipient compatibility, moisture content, and gel-sol transition temperature (GSTT). In-vitro and ex vivo release studies and antifungal activity against Candida albicans and Trichophyton rubrum were also carried out.</p><p><strong>Results: </strong>The measured viscosity (0.40±11 Pa.s), spreadability (14.98 ± 74 gm.cm/sec), and drug content (97.11 ± 27 %) of the optimized formulation were very close to the calculated values. The optimized organogel had a skin-compatible pH (6-7), high in-vitro (98.55±0.32%) and exvivo (88.95 ± 1.55 %) drug release, and a wider zone of inhibition (22 mm) against Candida albicans and Trichophyton rubrum than a commercial product.</p><p><strong>Discussion: </strong>The microwave-irradiation-synthesized BBD-optimized SN organogel with neem oil showed excellent drug release (>98%), skin-friendly pH, and improved antifungal activity (22 mm inhibition) compared to commercial preparations in line with green pharmaceutical trends. The scalability of microwave procedures, stability over extended periods, and sparse excipient screening require investigation to extend laboratory success to clinical application.</p><p><strong>Conclusion: </strong>The research was able to successfully develop and optimize a Sertaconazole Nitrate organogel through a microwave-assisted process with a natural oil. The optimized formulation was found to have good physicochemical parameters, drug release behavior, skin compatibility, and improved antifungal activity, which implies its suitability for effective topical drug delivery.</p>","PeriodicalId":94368,"journal":{"name":"Anti-inflammatory & anti-allergy agents in medicinal chemistry","volume":" ","pages":"44-61"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145484613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resveratrol as a Regulator of Inflammation, Oxidative Stress, and Glucose Stability in Diabetes.","authors":"Abdullah M Hasbullah, Ambika Binesh, Kaliyamurthi Venkatachalam","doi":"10.2174/0118715230414089250926071435","DOIUrl":"10.2174/0118715230414089250926071435","url":null,"abstract":"<p><p>Diabetes mellitus, a complex metabolic disorder, proves to be a challenge for the population around the world, as it includes chronic inflammation, oxidative stress, and glucose dysregulation. Resveratrol is a naturally occurring compound that acts as a diabetes complication- mitigating agent through complex cellular maneuvers and acts specifically to remedy diabetes complications. This review focuses on the action of resveratrol, underlining its vital impact in the alteration of significant pathophysiological pathways in diabetes. Besides, the activation of the AMPK and SIRT1 pathways also greatly improved cellular stress responses as well as insulin sensitivity. Numerous clinical studies have validated that the implementation of natural polyphenols' supplementation enhances glucose control, diabetes, inflammation, and insulin sensitivity, which supports these study findings. Its clear safety profile and flexible bioavailability also enhance its position as an adjunct diabetes therapy. As promising as that is, some issues still need to be addressed, including tackling standardized challenges like dosage issues and bioavailability.</p>","PeriodicalId":94368,"journal":{"name":"Anti-inflammatory & anti-allergy agents in medicinal chemistry","volume":" ","pages":"1-14"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145411166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microsponge Drug Delivery Systems: Advancing the Therapeutic Efficacy of Anti-Inflammatory Medications.","authors":"Akshay Kumar, Rakesh, Rajesh Gautam, Vir Vikram","doi":"10.2174/0118715230401202251106104630","DOIUrl":"10.2174/0118715230401202251106104630","url":null,"abstract":"<p><p>Microsponge drug delivery systems represent an innovative approach to enhancing the therapeutic efficacy of anti-inflammatory drugs through controlled release, improved bioavailability, and targeted delivery. These porous polymeric microspheres, ranging from 5 to 300 μm, encapsulate active pharmaceutical ingredients (APIs) to achieve sustained drug release, minimizing systemic side effects and dosing frequency. Chronic inflammatory conditions, such as rheumatoid arthritis, inflammatory bowel disease (IBD), and psoriasis, often require prolonged treatment, but conventional therapies such as nonsteroidal anti-inflammatory drugs (NSAIDs) and corticosteroids are limited by systemic toxicity and frequent administration. Microsponges address these challenges by enhancing drug stability, increasing retention at the target site, and reducing systemic exposure. Advanced fabrication techniques, including emulsion solvent diffusion, liquid-liquid suspension polymerization, and electrohydrodynamic atomization, allow precise control over microsponge properties, optimizing drug loading and release kinetics. In arthritis, microsponges extend anti-inflammatory activity; in IBD, they enable colon-specific delivery; and in psoriasis, they enhance drug penetration through keratinized plaques. These systems improve patient compliance by reducing dosing frequency and minimizing adverse effects. Despite their promise, further research is needed to optimize formulations, evaluate long-term safety, and fully explore their potential in managing chronic inflammatory diseases. Microsponge technology offers a transformative platform for improving therapeutic outcomes, paving the way for innovative treatments in pharmaceutical and clinical applications.</p>","PeriodicalId":94368,"journal":{"name":"Anti-inflammatory & anti-allergy agents in medicinal chemistry","volume":" ","pages":"79-90"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145703584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation, Characterization, Molecular Docking, and Antioxidant Evaluation of Esculetin Derived from <i>Cichorium intybus</i> Seeds.","authors":"Naina, Phool Chandra","doi":"10.2174/0118715230393322250911114129","DOIUrl":"10.2174/0118715230393322250911114129","url":null,"abstract":"<p><strong>Introduction: </strong>Cichorium intybus, a biennial plant belonging to the Asteraceae family, has been widely utilized in traditional Indian medicine for its tonic, anti-acne, anti-inflammatory, antioxidant, and hepatoprotective properties. Despite its known medicinal benefits, the bioactive compounds responsible for these activities require further exploration to validate their therapeutic potential. Our aim is to investigate the molecular docking interactions and antioxidant potential of an isolated bioactive compound from Cichorium intybus seeds, with a focus on its role in mitigating oxidative stress and inflammation in the liver.</p><p><strong>Methods: </strong>The compound was isolated using ethanol extraction, followed by phytochemical screening, TLC, column chromatography, and identification through FTIR, NMR, and mass spectroscopy. Molecular docking studies were conducted using Schrödinger Suite to analyze interactions with PPARα. Antioxidant activity was evaluated using DPPH and ABTS radical scavenging assays, with results compared through Trolox Equivalent Antioxidant Capacity (TEAC) values.</p><p><strong>Results: </strong>Esculetin, the isolated compound, exhibited strong binding affinity with PPARα (XP GScore: -7.0 kcal/mol). Antioxidant assays showed moderate activity, with DPPH radical scavenging activity (RSA) of 10.37% and ABTS RSA of 7.445%. The TEAC values were 13.23 μmol/mg and 21.930 μmol/mg, respectively, indicating its potential antioxidant efficacy.</p><p><strong>Discussion: </strong>Esculetin from Cichorium intybus showed moderate antioxidant activity and strong PPARα binding, indicating its potential as a therapeutic agent. These findings align with existing research but require validation through in vivo studies to confirm efficacy and elucidate biological mechanisms.</p><p><strong>Conclusion: </strong>Esculetin demonstrates significant potential as a bioactive antioxidant and antiinflammatory agent, supporting its relevance for further pharmacological and therapeutic investigations.</p>","PeriodicalId":94368,"journal":{"name":"Anti-inflammatory & anti-allergy agents in medicinal chemistry","volume":" ","pages":"62-72"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145234727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis and Biological Activity of Guanidine Scaffold: A Comprehensive Review.","authors":"Surendra Kumar Verma, Shweta Singh Verma, Amit Verma, Manshi Saxsena, Ajay Kumar, Vishnu Dev Verma","doi":"10.2174/0118715230387213251208182129","DOIUrl":"10.2174/0118715230387213251208182129","url":null,"abstract":"<p><p>Derivatives of guanidine are found to be highly informative for their design in antibacterial and anticancer applications, thus positioning them as promising future drug candidates. This study evaluates and synthesizes a diverse group of guanidine derivatives, with a focus on those bearing cyclic aryl and acyl groups. The prepared compounds showed strong antifungal and antibacterial activities with selectivity against Candida albicans and Gram-positive bacteria. The Structure-Activity Relationship analysis showed that substituent groups are important for biological activity, and n-propyl linkages achieved an optimal balance between hemolytic and antibacterial properties. Additionally, the antiproliferative activities of all synthesized compounds (110a-110v) were assessed in Michigan Cancer Foundation-7 (MCF-7) human breast cancer cells. The Structure Activity Relationship study results suggest that planned substitutions can significantly enhance biological activity, offering potential approaches to counter the growing threat of antibiotic resistance.</p>","PeriodicalId":94368,"journal":{"name":"Anti-inflammatory & anti-allergy agents in medicinal chemistry","volume":" ","pages":"143-176"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147944321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Next-Generation Probiotics in Allergy Therapy: Scientific Evidence and Clinical Applications.","authors":"Vatan Chaudhary, Atul Pratap Singh, Himanchal Sharma, Dhananjay Taumar","doi":"10.2174/0118715230407403251105101126","DOIUrl":"10.2174/0118715230407403251105101126","url":null,"abstract":"<p><p>The global increase in allergic diseases, such as atopic dermatitis, allergic rhinitis, asthma, and food allergies, has become a major public health issue. These diseases typically involve immune dysregulation, including a Th1/Th2 imbalance, increased IgE levels, regulatory T cell (Treg) dysfunction, and epithelial barrier dysfunction. New research has recognised an important role for the gut and mucosal microbiome in regulating immune responses and has prompted interest in the therapeutic utility of probiotics. Probiotics are live microbes that, when given in adequate amounts, confer health benefits, generally such as immunomodulation or restoration of gut barrier function. Traditional probiotics (i.e., Lactobacillus and Bifidobacterium species) reduce allergic inflammation through promotion of Treg differentiation, increases in antiinflammatory cytokines (e.g., IL-10), suppression of Th2 cytokines (e.g., IL-4), and modification of IFNγ. Traditional probiotics also support mucosal barrier function and restore microbial composition by producing short-chain fatty acids (SCFAs), like butyrate, which act directly on Gprotein- coupled receptors and histone deacetylases to suppress inflammation. Next-generation probiotics (NGPs), such as Akkermansia muciniphila, Faecalibacterium prausnitzii, Bacteroides fragilis, and some clusters of Clostridia, can provide more targeted effects. These NGPs can secrete anti-inflammatory metabolite compounds, such as polysaccharide A (PSA), which modulate dendritic cells and increase Treg activity, and can promote mucin production to improve gut barrier function. Overall, there are key issues with strain specificity, dose, safety in immunocompromised individuals, and possible regulatory classification issues. Future opportunities may include precision microbiome profiling, synthetic biology, and artificial intelligence-driven strain discovery to develop personalised approaches to allergy immunotherapy.</p>","PeriodicalId":94368,"journal":{"name":"Anti-inflammatory & anti-allergy agents in medicinal chemistry","volume":" ","pages":"177-188"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145703534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated Virtual Screening Approaches to Identify Novel N- Substituted Heterocyclic Compounds as MAPK Inhibitors for Neuro Inflammation.","authors":"Aravind Kanniappan, Annapoorna Vadivelu","doi":"10.2174/0118715230406228251127094543","DOIUrl":"10.2174/0118715230406228251127094543","url":null,"abstract":"<p><strong>Introduction: </strong>The Mitogen-Activated Protein Kinase (MAPK) is an intricately linked signaling cascade that frequently contributes to drug resistance in neurodegenerative diseases. This study presents an integrated approach combining in silico molecular docking, ADMETbased screening, and evaluation to identify novel N-substituted heterocyclic compounds as MAPK inhibitors for neuroinflammation.</p><p><strong>Methods: </strong>The molecular docking of the designed 135 nitrogen-substituted 5-, 6-, and 7- membered derivatives was carried out using Flare GUI software, docked into the MAPK (p38, ERK, JNK) binding site with PDB code 3LN1. The interaction was evaluated based on the reranked score comparison between the designed derivatives and the co-crystallized ligand. Trametinib was used as the reference, and 8 selected compounds were evaluated for their ADMET profiling using Swiss ADME, Molinspiration, OSIRIS, and Protox II. The molecules were filtered for their drug-like properties.</p><p><strong>Results: </strong>The docking results showed that more than 6% of the designed compounds displayed a good binding score compared with the reference Trametinib. The three targets used for screening are P38-alpha MAP kinase (2BAL), mitogen-activated protein kinase 1 ERK (3I5Z), and JNKinteracting protein 1 (7NYK). A maximum binding score of -8 kcal/mol was found for P38-alpha MAP kinase (2BAL).</p><p><strong>Discussion: </strong>ADMET screening gave 13 selected compounds that displayed affirmative pharmacokinetic and pharmacodynamic profiles, paving the way for many new drugs for development.</p><p><strong>Conclusion: </strong>There is a large scope for the optimization of the core, suggesting that the screened 8 molecules might be promising targets as inhibitors of P38-alpha MAP kinase in the MAPK pathway and are feasible to be synthesized.</p>","PeriodicalId":94368,"journal":{"name":"Anti-inflammatory & anti-allergy agents in medicinal chemistry","volume":" ","pages":"204-217"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147985043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Visfatin as a Potential Inflammatory Biomarker and Therapeutic Target in Osteoarthritis: A Narrative Review.","authors":"Utpal Bhui, Ashutosh Sengar, Bimlesh Kumar, Shantanu Bandopadhyay","doi":"10.2174/0118715230409497251105102359","DOIUrl":"https://doi.org/10.2174/0118715230409497251105102359","url":null,"abstract":"<p><p>Osteoarthritis (OA) is a common, chronic degenerative joint disease that leads to the progressive degeneration of articular cartilage, subchondral bone, and synovium. In short, it is characterized primarily by inflammation, cartilage breakdown, and subchondral bone remodeling leading to joint pain, stiffness, and severe functional limitations. OA pathogenesis results from complex reciprocal interactions between genetic, mechanical, and environmental factors that culminate in the activation of pro-inflammatory mediators such as Interleukin 1β (IL 1β) and Tumor Necrosis Factor alpha (TNF α), which stimulate Matrix Metalloproteinases (MMPs) and break down the cartilage extracellular matrix. Additionally, oxidative stress, mitochondrial dysfunction, and chondrocyte senescence play crucial roles in disease progression. Consequently, adipokines have become important contributors to OA pathophysiology, with special emphasis on visfatin. These molecules released from adipose tissue also represent a systemic proinflammatory signal, found at higher concentrations in OA synovial fluid, and contribute to cartilage degradation and worsening of clinical symptoms. Visfatin participates in various signaling pathways to further amplify inflammatory cascades and cartilage destruction. In this review, we explore the role of visfatin and its possible mechanisms contributing to the progression of OA.</p>","PeriodicalId":94368,"journal":{"name":"Anti-inflammatory & anti-allergy agents in medicinal chemistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145703563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>In Vitro</i> Evaluation of the Synergistic Anti-inflammatory Activities of the Combined Hydroalcoholic Extracts from <i>Mentha pulegium</i> L. and <i>Pimpinella anisum</i> L.","authors":"Amina Tabet Zatla, Amina Hammoudi, Mohammed El Amine Dib, Joëlle Perard","doi":"10.2174/0118715230363843250122051332","DOIUrl":"10.2174/0118715230363843250122051332","url":null,"abstract":"<p><strong>Background: </strong>Chronic inflammatory diseases are rising, driving the search for effective natural treatments. <i>Mentha pulegium</i> L. and Pimpinella anisum L. (anise) exhibit notable anti-inflammatory properties individually, but their combined effects are less studied. This research evaluates the in vitro synergistic anti-inflammatory activities of their hydroalcoholic extracts. Phytochemical analysis confirmed the presence of flavonoids, tannins, and polyphenols in both extracts. Individually, they demonstrated significant activity (78.5% and 72.3%, respectively, at 10 g/L) compared to Diclofenac (62.3%). Their combination achieved 88.6% inhibition at the same concentration. These findings highlight their potential as natural anti-inflammatory agents.</p><p><strong>Introduction: </strong>The rise in chronic inflammatory diseases has increased interest in natural anti-inflammatory treatments. <i>Mentha pulegium</i> L. and Pimpinella anisum L. are wellknown for their anti-inflammatory potential, attributed to their bioactive compounds like flavonoids and polyphenols. While the individual effects of these plants are established, their combined use is underexplored. This study evaluates the in vitro synergistic antiinflammatory activity of hydroalcoholic extracts from these plants, aiming to offer an effective natural therapeutic alternative.</p><p><strong>Methods: </strong>Hydroalcoholic extracts were prepared by maceration of both plants. Anti-inflammatory activity was assessed using the protein denaturation method with Diclofenac as a standard.</p><p><strong>Results: </strong>Phytochemical analysis identified flavonoids, alkaloids, tannins, glycosides, and polyphenols in <i>Mentha pulegium</i>, with an absence of saponins. Pimpinella anisum contained flavonoids, tannins, heterosides, and polyphenols. The extracts exhibited strong anti-inflammatory activity individually (78.5% and 72.3%, respectively, at 10 g/L) and even higher inhibition (88.6%) when combined, surpassing Diclofenac (62.3%).</p><p><strong>Conclusion: </strong>The combination of <i>Mentha pulegium</i> and <i>Pimpinella anisum</i> extracts significantly enhanced anti-inflammatory activity compared to individual extracts, underscoring their potential as natural therapeutic alternatives to conventional treatments.</p>","PeriodicalId":94368,"journal":{"name":"Anti-inflammatory & anti-allergy agents in medicinal chemistry","volume":" ","pages":"215-220"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143461549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}