{"title":"Psilocybin Mushrooms and Public Health in Brazil: Insights from a Retrospective Analysis of Adverse Events and Their Implications for Regulatory Discussions.","authors":"Marcel Nogueira, Solimary García-Hernández, Gleicy Sotéro Roberto, Leonardo Zanella Marques","doi":"10.1615/IntJMedMushrooms.2024057053","DOIUrl":"10.1615/IntJMedMushrooms.2024057053","url":null,"abstract":"<p><p>Current drug policy classifies psilocybin, a compound found in psychoactive mushrooms, as having high abuse potential while overlooking its therapeutic properties. We evaluated the risk of psilocybin mushrooms to Brazilian public health compared to other toxic agents and assessed the need for regulatory discussions. This retrospective cross-sectional study followed STROBE guidelines, using data from the Notifiable Diseases Information System (SINAN) on adverse events reported from 2007 to 2022. Participants were categorized into a general drug abuse group, which was further divided into psilocybin and unknown mushroom subgroups. Clinical outcomes included non-hospitalization, hospitalization, and death, with associations analyzed via the Chi-square test. Out of 112,451 individuals seeking medical attention for drug abuse-related events, men predominated (n = 79,514; 70.7%), with alcohol being the primary agent (n = 71,824; 49.2%). The psilocybin mushroom group included 13 participants, and the unknown mushroom group included 51. Hospitalization rates were 19.5% (n = 21,923) for drug abuse, 46.2% (n = 6) for psilocybin mushrooms (0.02% of all hospitalizations) (99% CI: 10.6%-81.6%), and 23.5% (n = 12) for unknown mushrooms (99% CI: 8.3%-38.7%). Mortality was 1.8% (n = 2035) for drug abuse group, with no fatal events in the psilocybin or unknown mushroom groups. Deaths were mainly linked to cocaine (33.3%). These findings suggest a low risk for psilocybin mushrooms, though underreporting may be a factor. This study underscores the need for evidence-based regulatory discussions to ensure safe access to psilocybin for clinical and ceremonial use.</p>","PeriodicalId":94323,"journal":{"name":"International journal of medicinal mushrooms","volume":"27 2","pages":"13-37"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143653086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Şanlı Kabaktepe, Celal Bal, Emre Cem Eraslan, Ayşenur Gürgen, Ilgaz Akata, Mustafa Sevindik
{"title":"Evaluation of Bioactive Potential of the Ruby Bolete Hortiboletus rubellus (Agaricomycetes): Antioxidant, Enzyme Inhibition, and Antiproliferative Effects.","authors":"Şanlı Kabaktepe, Celal Bal, Emre Cem Eraslan, Ayşenur Gürgen, Ilgaz Akata, Mustafa Sevindik","doi":"10.1615/IntJMedMushrooms.2025059728","DOIUrl":"https://doi.org/10.1615/IntJMedMushrooms.2025059728","url":null,"abstract":"<p><p>This study comprehensively evaluates the antioxidant, enzyme inhibition, antiproliferative and phenolic compound analyses of the mushroom species Hortiboletus rubellus. Samples were collected from oak forests in Yalova province of Turkey and extracted under appropriate laboratory conditions. Antioxidant capacity assessments were carried out using total antioxidant status (TAS), total oxidant status (TOS), DPPH radical scavenging activity and ferric reducing antioxidant power (FRAP) tests. The results show that H. rubellus has a significant antioxidant potential. Within the scope of enzyme inhibition analyses, the inhibition potential on acetylcholinesterase (AChE), butyrylcholinesterase (BChE), α-amylase and α-glucosidase enzymes was evaluated and it was determined that the mushroom showed promise in terms of neuroprotective and antidiabetic effects. In antiproliferative activity tests, H. rubellus was found to have dose-dependent cytotoxic effect on A549 lung cancer cell line. In addition, phenolic component analyses were performed by LC-MS/MS method and it was determined that the mushroom contains phenolic components important for health such as gallic acid, protocatechuic acid, quercetin. The findings show that H. rubellus has the potential to be used in functional food and pharmaceutical areas.</p>","PeriodicalId":94323,"journal":{"name":"International journal of medicinal mushrooms","volume":"27 10","pages":"21-31"},"PeriodicalIF":1.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144765964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kumar Katragunta, Bharathi Avula, Amar G Chittiboyina, Hemant Lata, Ikhlas A Khan
{"title":"Quantitative LC-QToF-MS Analysis of Mycochemicals in Amanita muscaria, Psilocybe spp. (Agaricomycetes), and Consumer Products.","authors":"Kumar Katragunta, Bharathi Avula, Amar G Chittiboyina, Hemant Lata, Ikhlas A Khan","doi":"10.1615/IntJMedMushrooms.2024056373","DOIUrl":"10.1615/IntJMedMushrooms.2024056373","url":null,"abstract":"<p><p>The psychedelic mushroom market has expanded rapidly due to changing regulations and increasing consumer demand. Product diversity now extends beyond traditional capsules and tablets to include gummies, powders, and confectionery items, complicating quality control efforts. To assess the quality and potential adulteration of Amanita musca-ria and Psilocybe cubensis-based products, a validated LC-QToF-MS method was developed. This method focused on five characteristic compounds: ibotenic acid (IBA), muscimol (MUS), muscarine, psilocin, and psilocybin that are constituents of A. muscaria and P. cubensis mushrooms. Method validation demonstrated satisfactory linearity, precision, and recovery of all five analytes. Psilocin and psilocybin levels ranged from 0.001-1.6% and 9.9-19.3%, respectively, in five Psilocybe species samples, while IBA, MUS, and muscarine levels in two samples of Amanita muscaria were 0.03-0.04%, 0.01- 0.02%, and 0.01-0.02%, respectively. By comparing commercial products to authentic samples, we evaluated the overall quality of 27 across various formulations. Our analysis included 14 gummies, three chocolates, six capsules, one tablet, and three powders. Although 11 of 14 gummies claimed to contain Amanita mushroom extracts, only MUS and muscarine were detected, without IBA. Interestingly, one gummy product indicated the presence of psilocin and psilocybin despite the labeling that claimed, \"no psilocybin.\" Eleven products contained psilocin and psilocybin as anticipated, but five products lacked all target compounds. These findings underscore the need for standardized product specifications. Nevertheless, the established LC-QToF-MS approach could serve as a valuable tool for evaluating the quality of magic mushroom-based consumer products.</p>","PeriodicalId":94323,"journal":{"name":"International journal of medicinal mushrooms","volume":"27 1","pages":"29-39"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142883833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A Tribute to Professor Shu-Ting Chang on his 95th Birthday.","authors":"Solomon P Wasser","doi":"10.1615/IntJMedMushrooms.v27.i10.10","DOIUrl":"https://doi.org/10.1615/IntJMedMushrooms.v27.i10.10","url":null,"abstract":"","PeriodicalId":94323,"journal":{"name":"International journal of medicinal mushrooms","volume":"27 10","pages":"1-5"},"PeriodicalIF":1.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144765945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yang Liu, Cong Xia, Bo Jie Chen, Xia Li, Xiaojian Wu, Amin Ismail, Xinhong Dong, Hock Eng Khoo
{"title":"Identification of Selected Flavonoids Extracted from Cap and Stem of Wild and Cultivated Ganoderma Species (Agaricomycetes) and Bioactivities.","authors":"Yang Liu, Cong Xia, Bo Jie Chen, Xia Li, Xiaojian Wu, Amin Ismail, Xinhong Dong, Hock Eng Khoo","doi":"10.1615/IntJMedMushrooms.2025058111","DOIUrl":"10.1615/IntJMedMushrooms.2025058111","url":null,"abstract":"<p><p>The objective of this study was to assess the bioactivities of flavonoids extracted from two distinct sections of the fruiting bodies of Ganoderma species. The flavonoids were extracted from the cap and stem of four Ganoderma species using a methanolic extraction method. The extraction rate, total flavonoid content, antioxidant activities, in vitro hypoglycemic effects, and antimicrobial activity of flavonoids derived from the wild and cultivated Ganoderma extracts were determined and analyzed. The findings indicated that the extract derived from cultivated black Ganoderma stem exhibited the most favorable outcomes among the four sample extracts. The lowest EC50 value of the DPPH radical scavenging assay was 36.9 μg/mL, and the extract demonstrated the highest inhibitory activity of α-glucosidase (35.88 μg/mL). However, the cap extract of the cultivated black Ganoderma demonstrated the highest inhibitory activity of α-amylase, with the IC50 value of 34.69 μg/mL. The cap extracts of the cultivated red Ganoderma exhibited the most pronounced antimicrobial activity. These findings indicate notable differences in the bioactivities of flavonoids extracted from the caps and stems of the four Ganoderma species. Consequently, the Ganoderma flavonoids have the potential to serve as potent bioactive ingredients with disease-prevention properties.</p>","PeriodicalId":94323,"journal":{"name":"International journal of medicinal mushrooms","volume":"27 6","pages":"61-79"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143660273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Larissa Trierveiler-Pereira, Amanda Prado-Elias, Juliano M Baltazar
{"title":"Adverse Health Effects Associated with the Consumption of Macrocybe titans (Agaricomycetes) from Brazil: A Case Report.","authors":"Larissa Trierveiler-Pereira, Amanda Prado-Elias, Juliano M Baltazar","doi":"10.1615/IntJMedMushrooms.2025058005","DOIUrl":"10.1615/IntJMedMushrooms.2025058005","url":null,"abstract":"<p><p>Mushrooms are an important source of food for animals. However, some species produce toxic compounds for humans. Moreover, some people can experience adverse health effects even when the consumed species is edible. Macrocybe titans, commonly known as the giant-mushroom, is a Neotropical species eaten by traditional and urban groups of people. In January 2022, a dinner meal was prepared using wild mushrooms of M. titans collected in a lawn in the urban area of Sorocaba (State of São Paulo, Southeastern Brazil). Some of the people who attended the dinner experienced adverse health effects (headache, nausea, abdominal pain, diarrhea, and vomiting) three to eight hours after the consumption of the mushrooms. Fortunately, none of them were hospitalized, and we believe that symptoms were caused due to a combination of factors. This work aims to report for the first time in literature the occurrence of adverse health effects after consuming M. titans.</p>","PeriodicalId":94323,"journal":{"name":"International journal of medicinal mushrooms","volume":"27 5","pages":"83-88"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143653089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ezeibe Chidi Nwaru, Nkechi P Oyeabor-Chinedum, Chinaza Maureen Ngozi, Chikodi Lilian Okechukwu, Prince Onyedinma Ukaogo, Nnamdi Ogwo, Chiemeriem Matthew Ahaiwe, Eziuche Amadike Ugbogu, Onyekachi Fa Ibiam
{"title":"Evaluation of Proximate Compositions, Morphology, and Trace Metal Profile of the Lung Oyster Mushroom Pleurotus pulmonarius (Agaricomycetes) Cultivated on Engine Oil-Polluted Soil Covered with Andropogon gayanus Grass Straw in Nigeria.","authors":"Ezeibe Chidi Nwaru, Nkechi P Oyeabor-Chinedum, Chinaza Maureen Ngozi, Chikodi Lilian Okechukwu, Prince Onyedinma Ukaogo, Nnamdi Ogwo, Chiemeriem Matthew Ahaiwe, Eziuche Amadike Ugbogu, Onyekachi Fa Ibiam","doi":"10.1615/IntJMedMushrooms.2025058657","DOIUrl":"https://doi.org/10.1615/IntJMedMushrooms.2025058657","url":null,"abstract":"<p><p>The growth, proximate composition, and trace metal profile of Pleurotus pulmonarius cultivated on oil-polluted soil covered with Andropogon gayanus grass straw were evaluated. The soil, polluted with 200 g/ wv condemned engine oil (8% pollution), was covered with 5 and 10 cm of grass straw, and the P. pulmonarius spawn inoculated. The mushroom growth was monitored over 21 days, measuring cap size, stipe length, stipe girth, and fresh weight for two harvests. Cap sizes ranged from 4.94 to 7.10 cm and 4.5 to 7.36 cm; stipe lengths, from 2.3 to 5.86 cm and 1.07 to 6.22 cm; stipe girths, from 3.04 to 3.93 cm and 1.77 to 5.08 cm; fresh weights, from 31.69 to 65 g/kg and 40.33 to 55 g/kg. At P ≤ 0.05, there was no significant difference in the growth parameters measured, nor was there any effect of crude oil pollution on the growth of the mushrooms. The mushroom fruiting bodies were also screened for possible presence of trace metals such as Fe (23.29-37.95 mg/kg); Zn (14.12-21.80 mg/kg); Pb (0.03-0.06 mg/kg); Cd (0.02 -0.032 mg/kg); Cr (0-0.05 mg/kg); and As (0-0.03 mg/kg). Comparing the trace metals, especially Pb, Cd, and Cr, which are detrimental to human health, with the provisional tolerable weekly intake (PTWI) that a consumer may take, it was shown that all the trace metals were within the provisional tolerable limit intake.</p>","PeriodicalId":94323,"journal":{"name":"International journal of medicinal mushrooms","volume":"27 7","pages":"85-95"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144061710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Boosting Nutritional Proficiency of Oyster Culinary-Medicinal Mushroom, Pleurotus ostreatus (Agaricomycetes): A Selenium and Zinc Fortification Exploration.","authors":"Riya Dhiman, Deepika Sud, Nidhi Ranaut","doi":"10.1615/IntJMedMushrooms.2024057506","DOIUrl":"10.1615/IntJMedMushrooms.2024057506","url":null,"abstract":"<p><p>The present study was aimed at evaluating the nutritional characteristics of bio-enriched oyster mushrooms. Cereal, leguminous and oilseed waste was used as substrates. Rice, soybean and mustard straw performed the best among all substrate fortified by adding Se and Zn salts individually and in combination at three different doses (25, 50 and 75 mg/kg) for nutrient analysis. The results showed that highest carbohydrate, 54.01 percent and protein content, 29.41 percent after fortification with Se and Zn at 75 mg/kg as compared to other doses on different substrates. Se at 75 mg/kg gave maximum carbohydrate (60.29%) and protein content (32.32%) in soybean straw while Zn supplementation at 50 mg/kg resulted in high fat content (2.74%) while rice straw had maximum fiber content (18.12%). Based on maximum yield, best dose of both the salts (25 mg/ kg of substrate) were combined and evaluated. The results indicated that soybean straw had the highest nutrient contents having 55.00 percent carbohydrate and 27.72 percent protein content. It was concluded that fortification with selenium and zinc increased the nutritional composition of Pleurotus ostreatus in comparison to control.</p>","PeriodicalId":94323,"journal":{"name":"International journal of medicinal mushrooms","volume":"27 3","pages":"23-33"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143019471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tomaž Trobec, Ema Kurešepi, Larisa Lara Popošek, Luka Šparl, Robert Frangež, Matej Skočaj, Kristina Sepčić
{"title":"Wild Mushrooms as a Source of Cholinesterase and Glutathione S-Transferase Inhibitors.","authors":"Tomaž Trobec, Ema Kurešepi, Larisa Lara Popošek, Luka Šparl, Robert Frangež, Matej Skočaj, Kristina Sepčić","doi":"10.1615/IntJMedMushrooms.2025058221","DOIUrl":"10.1615/IntJMedMushrooms.2025058221","url":null,"abstract":"<p><p>Several fungal species, including mushrooms, produce bioactive and toxic compounds that exert many activities, such as direct action on DNA, inhibition of protein synthesis, damage to cell membranes, or direct suppression of certain enzymatic activities. This is particularly important as mushrooms can play an important role in human nutrition and/or are used as dietary supplements. In this context, we screened ethanolic extracts of 64 wild mushrooms for their potential to inhibit cholinesterases and glutathione S-transferase. Seven of the 64 extracts showed an inhibitory effect on cholinesterases, while 24 inhibited glutathione S-transferase. These results suggest that the toxic effects of some mushrooms may be directly linked to a specific class of compounds that act as inhibitors of physiologically relevant enzymes. Most of the identified activities are described for the first time and call for caution, especially when the human diet is rich in mushrooms. Conversely, these bioactive molecules may serve as inspiration for chemical synthesis and the development of potential drugs to combat neurodegenerative diseases or cancer.</p>","PeriodicalId":94323,"journal":{"name":"International journal of medicinal mushrooms","volume":"27 6","pages":"23-34"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143660276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Sanguinoderma rugosum (Agaricomycetes), a Wild Malaysian Medicinal Mushroom, Triggers Anti-Neuroinflammatory Genes Expression in LPS-Stimulated BV-2 Microglial Cells.","authors":"Pui-Mun Chan, Yee-Shin Tan, Kek-Heng Chua, Vikineswary Sabaratnam, Umah Rani Kuppusamy","doi":"10.1615/IntJMedMushrooms.2025058986","DOIUrl":"https://doi.org/10.1615/IntJMedMushrooms.2025058986","url":null,"abstract":"<p><p>Sanguinoderma rugosum (synonym: Amauroderma rugosum) is a wild medicinal mushroom reported to prevent epileptic episodes and constant crying in babies within indigenous communities in Malaysia. It also has potential applications in the management of oxidative-related diseases. The aim of this study was to reveal the potential candidate genes in understanding the neuroinflammatory signaling pathways modulated by S. rugosum in LPS-stimulated BV-2 cells using microarray technology. This study showed that the hexane fraction (HF) of S. rugosum regulated 10 signaling pathways such as macrophage markers, MAPK, IL-1, oxidative damage, cytokines and inflammatory response, toll-like receptor, p38 MAPK, complement activation classical pathway, complement and coagulation cascades, and TNF-α NF-κB. HF downregulated the gene expression of inflammatory cytokines such as IL-1β, IL-1α, and IL-6, as well as other pro-inflammatory signaling intermediates such as Traf1, Traf2, Cd14. Conversely, it upregulated the anti-inflammatory genes such as Nfkbia and Nfkbie. Besides, HF reduced the nitric oxide (NO) levels in LPS-stimulated BV-2 cells. Taken together, these findings showed that HF of S. rugosum has anti-neuroinflammatory properties and could serve as baseline study for future investigation on the gene expression validation.</p>","PeriodicalId":94323,"journal":{"name":"International journal of medicinal mushrooms","volume":"27 8","pages":"1-12"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144218090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}