International journal of medicinal mushrooms最新文献

{"title":"Lion's Mane Medicinal Mushroom Hericium erinaceus (Agaricomycetes) Polysaccharides for Improved In Vitro Probiotic Growth, Adhesion, Antioxidant Activity, and Cryoprotective Properties.","authors":"Worrapot Pengpa, Tanes Sangsri, Prissana Wiriyajitsomboon, Patcharaporn Siwayaprahm","doi":"10.1615/IntJMedMushrooms.2025061287","DOIUrl":"10.1615/IntJMedMushrooms.2025061287","url":null,"abstract":"<p><p>Hericium erinaceus, commonly known as lion's mane mushroom, is a rare edible and medicinal fungus that is highly valued in both the culinary and medical fields. Its primary bioactive component, polysaccharides, holds significant therapeutic potential, particularly in healthcare. This study investigates the prebiotic effects of crude polysaccharides extracted from H. erinaceus on various probiotic strains. Three distinct fractions of crude polysaccharides, HEP-30, HEP-50, and HEP-80 were obtained through fractional precipitation using 30%, 50%, and 80% (v/v) ethanol concentrations from hot water-soluble extracts. The effects of the polysaccharide extracts on probiotic growth, adhesion, antioxidant activity, and cryoprotective properties were evaluated in vitro. Of the HEP fractions, HEP-80 contains the highest amounts of protein, total sugars, and reducing sugars. Galactose is the predominant monosaccharide in all HEP fractions. The polysaccharide extract exhibited antioxidant activity by inhibiting DPPH free radicals across all three tested concentrations, with an average IC50 value of 10.189 mg/mL. HEP-80 significantly enhanced the growth of all three probiotic strains compared with an unsupplemented control group. HEP-80 at a concentration of 50 μg/mL increased the adhesion of Lactiplantibacillus plantarum strain L47-2 by up to 30%. Furthermore, HEP compounds demonstrated cryoprotective properties, resulting in over 70% survival of L. plantarum strain L47-2 after 90 days of storage at -20°C following freeze-drying. H. erinaceus-derived crude polysaccharides hold promise as both prebiotics and cryoprotectants, making them a potentially valuable functional ingredient for use in food products.</p>","PeriodicalId":94323,"journal":{"name":"International journal of medicinal mushrooms","volume":"28 2","pages":"47-61"},"PeriodicalIF":1.4,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146151606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactives of Lion's Mane Medicinal Mushroom Hericium erinaceus (Agaricomycetes) Targeting PPAR Signaling Pathway: An Experimental and In Silico Investigation.","authors":"S Mahema, Jency Roshni, V Janakiraman, Sheikh F Ahmad, Sabry M Attia, Shiek S S J Ahmed","doi":"10.1615/IntJMedMushrooms.2025062333","DOIUrl":"10.1615/IntJMedMushrooms.2025062333","url":null,"abstract":"<p><p>Hericium erinaceus is a widely consumed edible mushroom with significant ethnopharmacological value. Nevertheless, the pharmacological understanding of H. erinaceus presenting crucial bioactive compounds, their therapeutic targets, and its benefits across diseases in the form of bioactive-target-disease connectivity is yet to be explored. This study evaluates the antioxidant activity and cytoprotective effects of H. erinaceus extract on V79 fibroblast cells in vitro, to profile its bioactive compounds, and to conduct a network pharmacological analysis to identify key bioactives linked to therapeutic target proteins and diseases. A qualitative analysis identified the existence of tannins, alkaloids, steroids, terpenoids, polysaccharides, and phytosterols. Vibrational infrared spectroscopy confirmed the existence of alkanes, alkenes, alkynes, aromatics, aldehydes, esters, alcohols, aliphatics, and primary amines. Cytoprotective investigation demonstrated that the concentrations (10-25 μg/mL) of the extract preserve 95% cell viability and significant antioxidant properties. Gas chromatography-mass spectrometry analysis of the H. erinaceus extract yielded 49 components. The absorption, distribution, metabolism, excretion, and toxicity (ADMET)-filtered compounds were subjected to target prediction and pathway analysis, which revealed PPARG protein and its signaling pathways were the most significant targets. Further, disease association analysis identified PPARG's links to various diseases, which could benefit from using H. erinaceus. Molecular docking (-6.804 kcal/mol), binding free energy (-42.05 kcal/mol), and dynamic simulations confirmed a strong interaction between PPARG and 2-methyl hexadecanoic acid. These findings underscore the antioxidant and cytoprotective capabilities of H. erinaceus phytochemicals, as well as their potential therapeutic safety and efficacy for further investigation.</p>","PeriodicalId":94323,"journal":{"name":"International journal of medicinal mushrooms","volume":"28 4","pages":"21-39"},"PeriodicalIF":1.4,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147597411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Network Pharmacology, Molecular Docking, and Molecular Dynamics Studies to Predict the Molecular Mechanisms of Cyclocybe cylindracea Mushroom (Agaricomycetes) Active Compounds for the Treatment of Breast Cancer.","authors":"Redouane Rebai, Mohammed Esseddik Toumi, Imene Derardja, Fethi Farouk Kebaili","doi":"10.1615/IntJMedMushrooms.2026064629","DOIUrl":"10.1615/IntJMedMushrooms.2026064629","url":null,"abstract":"<p><p>The study aimed to elucidate the molecular mechanisms by which active compounds from the mushroom Cyclocybe cylindracea exert anti-breast cancer effects, using network pharmacology and computational simulation. We identified potential mushroom compounds from the literature for target protein prediction. Several online tools and databases were used to collect the data on mushroom ingredient-related targets and their potential breast cancer-related genetic targets. Protein-protein interaction (PPI) networks and pathway enrichment analyses were performed. Finally, molecular docking and dynamic simulations were used to validate the binding affinity of the compounds to their protein targets. As a result, five potential compounds were identified, corresponding to 440 predicted targets. PPI network revealed potential targets, including AKT1, TP53, MAPK1, ESR1, HSP90AA1, MAPK3, and TNF. Furthermore, the functional enrichment analysis showed that the core targets were mainly enriched in the microRNA cancer pathway, the MAPK pathway, and the PI3K/AKT signaling pathway. Molecular docking analysis demonstrated that the breast cancer-related targets AKT1, MAPK1, and MAPK3 exhibited stronger binding affinity with rosmarinic acid than the other selected compounds. Additionally, molecular dynamics simulation revealed good stability for the best MAPK1-rosmarinic acid complex. In conclusion, our findings suggest that the active compounds of C. cylindracea may have anticancer potential by interfering with multiple signaling pathways.</p>","PeriodicalId":94323,"journal":{"name":"International journal of medicinal mushrooms","volume":"28 8","pages":"19-34"},"PeriodicalIF":1.4,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149187320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of a Tiger Milk Medicinal Mushroom (Lignosus rhinocerus TM02®)-Based Oral Gel for Pain Reduction and Healing in Minor Aphthous Stomatitis.","authors":"Aliza Nur Shuhaili Azlan, Ellysa Teng-Shan Leong, Janet Pei-Shan Chai, Betsy Sara Thomas, Nuruljannah Nor Azmi, Song-Haw Chong, Szu-Ting Ng, Chon-Seng Tan, Shin-Yee Fung, Yeannie Hui-Yeng Yap","doi":"10.1615/IntJMedMushrooms.2026064928","DOIUrl":"10.1615/IntJMedMushrooms.2026064928","url":null,"abstract":"<p><p>Recurrent aphthous stomatitis (RAS) is a common oral mucosal condition characterized by painful ulcerations with no consistently effective treatment. Lignosus rhinocerus (tiger milk mushroom) has been traditionally used in Southeast Asia for its wound-healing and anti-inflammatory properties, yet its clinical application for RAS remains insufficiently studied. This double-blind, randomized clinical trial evaluated the efficacy and safety of an oral gel containing L. rhinocerus TM02® (xLr®) extract in adults with minor RAS. Twenty-one participants aged 20 to 50 years were allocated to placebo gel (n = 6), xLr® gel (n = 7), or Kanolone Oral Base Paste (triamcinolone acetonide 0.1%) (n = 5). All gels were applied three times daily for 7 d. Pain intensity was recorded daily using a visual analog scale (VAS), ulcer diameter was measured on days 1, 3, 5, and 7, and epithelialization was assessed using the 3% hydrogen peroxide test. Kanolone produced the greatest reduction in pain (mean VAS: 2.40) and ulcer size (P ® (mean VAS: 3.10) and placebo (mean VAS: 3.93). The xLr® group demonstrated faster epithelialization (5.4 ± 0.61 d) compared with placebo (6.8 ± 0.40 d) and Kanolone (6.0 ± 0.63 d), although intergroup differences were insignificant. No adverse events were reported across all groups. These findings suggest that xLr® oral gel is safe and exhibits favorable trends in pain reduction and wound epithelialization, supporting ethnomedicinal claims of L. rhinocerus and indicating the need for larger confirmatory trials.</p>","PeriodicalId":94323,"journal":{"name":"International journal of medicinal mushrooms","volume":"28 8","pages":"77-87"},"PeriodicalIF":1.4,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149187400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microwave-Assisted Hydrogen Peroxide Synergistic Extraction of Auricularia auricula-judae (Agaricomycetes) Polysaccharides and Their Antioxidant Stress Response.","authors":"Jing Yao, Yuxuan Tang, Can Xiang, Han Shu, Shirong Li, Yingxuan Gu, Liyun Zhu, Yongjun Zhang","doi":"10.1615/IntJMedMushrooms.2026063268","DOIUrl":"https://doi.org/10.1615/IntJMedMushrooms.2026063268","url":null,"abstract":"<p><p>Oxidative stress, as the core pathological mechanism of imbalance in the body's oxidation-antioxidation system, is a key inducer of accelerated aging and degenerative diseases. This study, based on the synergistic principle of microwave energy and free radicals, constructed a microwave-assisted hydrogen peroxide (H2O2) synergistic system to optimize the extraction yield of Auricularia auricula-judae polysaccharide (AAPs-m) using the Box-Behnken design model based on single-factor experiments. A methyl viologen-induced oxidative stress model of Caenorhabditis elegans was established to evaluate the in vivo antioxidant stress capacity of AAPs-m. The results showed that the AAPs-m yield reached 36.76% ± 0.38% under the conditions of a liquid-to-solid ratio of 60.0 mL/g, a H2O2 concentration of 1.5% a microwave power of 400 W, and a temperature of 100°C for 61 min. AAPs-m is a heteropolysaccharide composed of mannose, glucuronic acid, glucose, galactose, and xylose, with a molar ratio of 53.82:6.10:25.50:2.36:11.44. Among them, the molar ratio of mannose is high, which is rare in AAPs extracted by other methods. AAPs-m significantly prolonged the lifespan of C. elegans under oxidative stress conditions (P P 2O2 synergistic technology, possesses antioxidant stress capabilities and has great potential and development value.</p>","PeriodicalId":94323,"journal":{"name":"International journal of medicinal mushrooms","volume":"28 5","pages":"13-27"},"PeriodicalIF":1.4,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149187160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective Effects of Lingzhi or Reishi Medicinal Mushroom Ganoderma lucidum (Agaricomycetes) Polysaccharides Extracted via Free Radical-Assisted Method on DSS-Induced Ulcerative Colitis in Mice.","authors":"Han Shu, Xuyang Liu, Fen Hang, Yingxuan Gu, Jiale Liang, Yike Wu, Lijun Chen, Yi Ji, Yongjun Zhang","doi":"10.1615/IntJMedMushrooms.2026063846","DOIUrl":"https://doi.org/10.1615/IntJMedMushrooms.2026063846","url":null,"abstract":"<p><p>This study investigated the protective effects of Ganoderma lucidum polysaccharides against ulcerative colitis (UC), addressing the challenges posed by the rigid cell wall structure and low solubility of its polysaccharides, as well as the unclear anti-ulcerative colitis potential. G. lucidum polysaccharides were extracted using a free radical-assisted extraction method, followed by ethanol precipitation and deproteinization. Monosaccharide composition and molecular weight were then analyzed. A dextran sulfate sodium (DSS)-induced UC mouse model was established to evaluate the protective effects of G. lucidum polysaccharides. Monosaccharide composition analysis revealed that G. lucidum polysaccharides primarily consisted of D-mannose, D-glucuronic acid, and D-glucose, with molar percentages of 2.49:2.36:88.16, respectively. The weight-average and number-average molecular weights were 50.017 and 22.851 kDa, respectively. Compared with the negative control group, G. lucidum polysaccharides mitigated DSS-induced weight loss and reduced the disease activity index (DAI) score in mice. G. lucidum polysaccharides significantly enhanced the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in serum (P < 0.05). Furthermore, G. lucidum polysaccharides significantly reduced the levels of inflammatory cytokines tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) in colonic tissues (P < 0.05) and alleviated the DSS-induced increase in colonic permeability. 16S rRNA sequencing of gut microbiota revealed that G. lucidum polysaccharides reduced the Firmicutes/Bacteroidetes (F/B) ratio elevated by DSS at the phylum level. At the genus level, G. lucidum polysaccharides intervention increased the abundance of beneficial bacteria such as Muribaculum. In conclusion, G. lucidum polysaccharides extracted using the free radical-assisted extraction method exhibit a protective effect against UC and holds significant potential for development.</p>","PeriodicalId":94323,"journal":{"name":"International journal of medicinal mushrooms","volume":"28 6","pages":"53-68"},"PeriodicalIF":1.4,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149187235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Scientometrics of Edible Medicinal Mushrooms and Fungi Topic Research Based on Web of Science.","authors":"Jie Sun, Bao-Zhong Yuan","doi":"10.1615/IntJMedMushrooms.2026066356","DOIUrl":"https://doi.org/10.1615/IntJMedMushrooms.2026066356","url":null,"abstract":"<p><p>Mushrooms possess high nutritional and medicinal values, are playing an increasingly important role in promoting human health worldwide. This study analyzed 9035 papers of medicinal or edible mushrooms and fungi topic research from 1907 to March 20, 2026 based on the Web of Science (WoS), papers mainly written in English (97.809%), were from 148 countries or regions, 6,142 organizations and published 1,375 journals and book series. International Journal of Medicinal Mushrooms published 1,358 (15.03%) papers. Top 5 countries or regions were P.R. China, India, Japan, US, Poland, each published more than 463 papers. Among top 20 countries and regions, Portugal, Czech Re- public, France, Spain and Italy, show the higher citations per paper which are more than 33.43 times per paper. The top five affiliations were Chinese Academy of Sciences, Chinese University of Hong Kong, Universiti Malaya, Shanghai Academy of Agricultural Sciences, University of Belgrade, each with more than 139 papers. Based on Essential Science Indicators (ESI) database, there are sixty top papers of all highly cited papers. All key words of the medicinal or edible mushrooms and fungi topic research were separated into six clusters for different research topic. The papers covered 15 Sustainable Development Goals (SDG), and the most papers are focused on five SDG of 03 Good Health and Well Being (6,770, 74.931%), 02 Zero Hunger (1018, 11.267%), 15 Life On Land (803, 8.888%), 06 Clean Water and Sanitation (281, 3.11%), 12 Responsible Consumption and Production (179, 1.981%). For the medicinal or Edible Mushrooms topic research, there is one new Emerging Topics by Horizon Navigator. The results will help researchers clarify the current situation but also provide guidance for future research.</p>","PeriodicalId":94323,"journal":{"name":"International journal of medicinal mushrooms","volume":"28 10","pages":"1-17"},"PeriodicalIF":1.4,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149303483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detrimental, Essential, and Nutritionally Nonessential Elements in Fruiting Bodies of Auricularia auricula-judae (Agaricomycetes) Collected in Localities with Different Environmental Burdens.","authors":"Jan Šíma, Martin Křížek, Jiří Krejsa, Martin Šeda, Barbora Vyhlídková, Lukáš Rokos, Lubomír Svoboda","doi":"10.1615/IntJMedMushrooms.2025061255","DOIUrl":"10.1615/IntJMedMushrooms.2025061255","url":null,"abstract":"<p><p>Selected detrimental, essential, and nutritionally nonessential elements (total of 26 elements) were determined in fruiting bodies of Auricularia auricula-judae taken from three localities with different environmental burdens: a sampling site with no direct sources of contamination, a site located near a heavily trafficked road, and a site located near an amphibolite quarry. The analysis revealed that proximity of the road led to increased contents (mg/kg of dry matter; compared with the site without any direct contamination) of Al (140 vs. 40), As (0.14 vs. 0.06), Ba (17 vs. 11), Ca (5600 vs. 2600), Co (0.17 vs. 0.07), Cr (0.51 vs. 0.28), Cu (4.6 vs. 3.4), Fe (200 vs. 120), Mn (32 vs. 21), Mo (0.31 vs. 0.15), Na (420 vs. 170), Ni (1.4 vs. 0.72), Pb (0.14 vs. 0.08), Sr (15 vs. 9.7), and V (0.33 vs. 0.12). Similarly, the amphibolite quarrying led to increased contents of Al (590 vs. 40), As (0.15 vs. 0.06), Ca (9300 vs. 2600), Co (0.27 vs. 0.07), Cr (2.8 vs. 0.28), Cu (6.2 vs. 3.4), Fe (400 vs. 120), Mg (2400 vs. 1800), Mo (0.60 vs. 0.15), Na (370 vs. 170), Ni (1.6 vs. 0.72), Pb (0.18 vs. 0.08), Sr (17 vs. 9.7), and V (0.80 vs. 0.12) in comparison with the site without direct contamination. The studied sources of contamination may substantially affect the element levels in A. auricula-judae fruiting bodies, and this must be considered if this mushroom is collected for culinary or alternative medicine purposes.</p>","PeriodicalId":94323,"journal":{"name":"International journal of medicinal mushrooms","volume":"28 2","pages":"83-97"},"PeriodicalIF":1.4,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146151656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenolic Profile and Biological Activities of Stropharia inuncta (Agaricomycetes) Extracts: Insights into Antioxidant, Enzyme Inhibition, and Cytotoxic Effects.","authors":"İskender Karaltı, Celal Bal, Emre Cem Eraslan, Ilgaz Akata, Mustafa Sevindik","doi":"10.1615/IntJMedMushrooms.2025061853","DOIUrl":"10.1615/IntJMedMushrooms.2025061853","url":null,"abstract":"<p><p>The phenolic compound profile of ethanolic extract of Stropharia inuncta (Fr.) Quél., a natural macrofungus species, and the effects of this chemical structure on antioxidant, anticholinesterase and antiproliferative activities were evaluated. Antioxidant capacity was determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), and Rel Assay Diagnostics kits; DPPH: 72.653 ± 1.857 mg Trolox equivalents (TE)/g, FRAP: 86.873 ± 1.125 mg TE/g, otal antioxidant status (TAS): 4.049 ± 0.057 mmol/L, total oxidant status (TOS): 9.642 ± 0.038 μmol/L, oxidative stress index (OSI): 0.238 ± 0.002, respectively. Within the scope of anticholinesterase activity, IC50 values of the extract on acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes were determined as 55.007 ± 1.411 μg/mL and 82.993 ± 1.501 μg/mL, respectively. Antiproliferative effect was evaluated on A549 human lung adenocarcinoma cell line after 24 h of incubation; significant decrease in cell viability was observed especially at concentrations of 100 and 200 μg/mL. Phenolic content was analyzed by LC-MS/MS method and the highest levels of gallic acid (3713.45 ± 12.26 mg/kg), quercetin (2911.40 ± 6.19 mg/kg), and 4-hydroxybenzoic acid (2167.69 ± 4.71 mg/kg) were detected. Also compounds such as vanillic acid, catechinhydrate and acetohydroxamic acid were found at significant levels. The findings show that S. inuncta is a rich natural source of phenolic compounds and this chemical structure contributes to versatile biological activities. The limited information in the literature on the biological activities and phenolic compound profile of this species positions the study as a contribution to the field.</p>","PeriodicalId":94323,"journal":{"name":"International journal of medicinal mushrooms","volume":"28 4","pages":"87-96"},"PeriodicalIF":1.4,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147597170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced Biological Efficiency of Indian Strains of the Culinary-Medicinal Lion's Mane Mushroom Hericium erinaceus (Agaricomycetes) for Health-Promoting Antioxidant Activities.","authors":"Krishna Kant Mishra, Ramesh Singh Pal, Pankaj Kumar Mishra, Gaurav Verma, Lakshmi Kant","doi":"10.1615/IntJMedMushrooms.2026063599","DOIUrl":"https://doi.org/10.1615/IntJMedMushrooms.2026063599","url":null,"abstract":"<p><p>The present investigation was carried out to identify a high yielding strain of Hericium erinaceus for its cultivation on locally available substrate, wheat straw and determination of health promoting antioxidant activities from the fruit bodies. The results showed that the yield was found significantly higher in H. erinaceus strain He 04 (88.89 kg/100 kg dry wheat substrate) followed by strain He 05 (47.22 kg/100 kg dry substrate). The polyphenol content in different strains varied from 1.74-5.21 mg GAE/100 g fresh weight with maximum in strain He 04 (5.21 mg GAE/100g fresh wt), however, flavonoid content was in the range of 2.12-4.31 mg quercitin equivalent/g fresh wt with highest in strain He 05 (4.31 mg quercitin equivalent/g fresh wt). The RSA on 2,2-diphenyl-1-picrylhydrazyl (DPPH) ranged between 21.7 and 43.25% with maximum activity in strain He 05 (43.25%) and 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) scavenging activity ranged between 45.67% and 77.32% with highest in strain He 04 (77.32%). Principal component analysis (PCA) revealed that strain 4 contained higher total phenolics, ABTS activity, total antioxidant activity, ferric reducing antioxidant power (FRAP) value, total number of fruiting bodies and yield while strain 5 shown with higher flavonoids and DPPH activity. Agglomerative hierarchical clustering analysis revealed that studied strains fall into two clusters; cluster I included three strains in two groups, group I included strains 1 and 2 and group II included only strain 3, while cluster II included strains 4 and 5. Out of five H. erinaceous strains studied, strain 4 recorded significantly higher yield as well as antioxidant activities. Commercial mushroom farms may adapt this strain in Indian conditions.</p>","PeriodicalId":94323,"journal":{"name":"International journal of medicinal mushrooms","volume":"28 6","pages":"23-34"},"PeriodicalIF":1.4,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149187227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}