Virology最新文献_第7页

Epidemiological characteristics of non-polio enteroviruses in respiratory infections: An Italian multicentre retrospective study, 2022-2023. 非脊髓灰质炎肠道病毒在呼吸道感染中的流行病学特征：一项意大利多中心回顾性研究，2022-2023 年。

Virology Pub Date : 2024-12-16 DOI: 10.1016/j.virol.2024.110302

Laura Pellegrinelli, Federica A M Giardina, Federica Novazzi, Elisa Vian, Valeria Biscaro, Cristina Russo, Stefania Ranno, Sara Uceda Renteria, Annapaola Callegaro, Elisabetta Pagani, Elisa Masi, Claudia Tiberio, Martina Esposito, Katia Marinelli, Stefano Menzo, Sandro Binda, Francesca Rovida, Nicasio Mancini, Anna Maria Colacicco, Maria Scarasciulli, Eleonora Lalle, Fabrizio Maggi, Giulia Piccirilli, Tiziana Lazzarotto, Antonio Piralla, Fausto Baldanti, Elena Pariani

引用次数: 0

Advances in the diagnostic techniques of African swine fever. 非洲猪瘟诊断技术进展。

Virology Pub Date : 2024-12-15 DOI: 10.1016/j.virol.2024.110351

Xin Zhang, Lei Zhou, Xinna Ge, Peng Gao, Qiongqiong Zhou, Jun Han, Xin Guo, Yongning Zhang, Hanchun Yang

{"title":"Advances in the diagnostic techniques of African swine fever.","authors":"Xin Zhang, Lei Zhou, Xinna Ge, Peng Gao, Qiongqiong Zhou, Jun Han, Xin Guo, Yongning Zhang, Hanchun Yang","doi":"10.1016/j.virol.2024.110351","DOIUrl":"https://doi.org/10.1016/j.virol.2024.110351","url":null,"abstract":"African swine fever (ASF) is a highly contagious disease of pigs caused by African swine fever virus, which poses a huge threat to the global swine industry and is therefore listed as a notifiable disease by the World Organization for Animal Health. Due to the global lack of safe and efficacious vaccines and therapeutic drugs, early diagnosis of cases, whether on-site or laboratory, are crucial for the prevention and control of ASF. Therefore, rapid and reliable diagnosis and detection have become the main means to combat ASF. In this paper, various diagnostic techniques developed globally for ASF diagnosis, including etiological, molecular biological and serological diagnostic techniques, as well as conventional and novel diagnostic techniques, were comprehensively reviewed, and the main advantages and disadvantages of currently commonly used diagnostic techniques were introduced. It is expected that this paper will provide references for selecting appropriate ASF diagnostic techniques in different application scenarios, and also provide directions for the development of innovative diagnostic techniques for ASF in the future.","PeriodicalId":94266,"journal":{"name":"Virology","volume":"603 ","pages":"110351"},"PeriodicalIF":0.0,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142857412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative analyses of three grapevine Pinot gris virus cDNA clones reveal insights into the pathological properties of different phylogroups. 三个葡萄灰比诺病毒cDNA克隆的比较分析揭示了不同系统群的病理特性。

Virology Pub Date : 2024-12-15 DOI: 10.1016/j.virol.2024.110360

Dipendra Karki, Rita Musetti, Baozhong Meng

{"title":"Comparative analyses of three grapevine Pinot gris virus cDNA clones reveal insights into the pathological properties of different phylogroups.","authors":"Dipendra Karki, Rita Musetti, Baozhong Meng","doi":"10.1016/j.virol.2024.110360","DOIUrl":"https://doi.org/10.1016/j.virol.2024.110360","url":null,"abstract":"Grapevine Pinot gris virus (GPGV) is an emerging grapevine virus associated with grapevine leaf mottling and deformation (GLMD) disease. Being a recently identified virus, the molecular biology, pathological properties, and etiological complexity of GPGV remain poorly studied. Previous research revealed that GPGV comprises genetically different variants, some encoding a larger movement protein (MP) and others a shorter MP due to a C/T polymorphic site in ORF2 encoding MP. Variants that encode the shorter MP are associated with severe disease, whereas variants encoding the longer MP are associated with mild or no symptoms. However, this has yet to be demonstrated experimentally. Here, we report the construction of a wildtype cDNA clone, pGPGV-SY, based on ON93-12, a local isolate from Syrah closely related to the variants encoding the larger MP. Surprisingly, our clone exhibited significantly faster replication and caused more severe disease symptoms than pRI::GPGV-lat, an Italian GPGV clone, with a longer MP and demonstrated similar efficacies with that of pRI::GPGV-vir, another Italian clone with a shorter MP. A single C to T mutation at the polymorphic site of pGPGV-SY resulted in a two-fold higher RNA accumulation in the grapevine. Findings from this work constitute a leap toward the long-standing and complex question pertaining to the relationship between GPGV variant groups and GLMD. Integrating findings from this work and those by others, we propose an updated model to explain the complex relationship between GPGV variants and GLMD.","PeriodicalId":94266,"journal":{"name":"Virology","volume":"603 ","pages":"110360"},"PeriodicalIF":0.0,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142873806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serologic differentiation between wild-type and cell-adapted African swine fever virus infections: A novel DIVA strategy using the MGF100-1L protein. 野生型和细胞适应型非洲猪瘟病毒感染的血清学分化：使用MGF100-1L蛋白的一种新的DIVA策略

Virology Pub Date : 2024-12-14 DOI: 10.1016/j.virol.2024.110349

Theeradej Thaweerattanasinp, Janya Saenboonrueng, Asawin Wanitchang, Kanjana Srisutthisamphan, Nathiphat Tanwattana, Ratchanont Viriyakitkosol, Challika Kaewborisuth, Anan Jongkaewwattana

{"title":"Serologic differentiation between wild-type and cell-adapted African swine fever virus infections: A novel DIVA strategy using the MGF100-1L protein.","authors":"Theeradej Thaweerattanasinp, Janya Saenboonrueng, Asawin Wanitchang, Kanjana Srisutthisamphan, Nathiphat Tanwattana, Ratchanont Viriyakitkosol, Challika Kaewborisuth, Anan Jongkaewwattana","doi":"10.1016/j.virol.2024.110349","DOIUrl":"https://doi.org/10.1016/j.virol.2024.110349","url":null,"abstract":"African swine fever virus (ASFV) poses a significant threat to the global swine industry and requires improved control strategies. Here, we developed a Differentiating Infected from Vaccinated Animals (DIVA) assay based on the MGF100-1L protein, which is absent in a cell-adapted ASFV strain lacking several multigene family (MGF) genes. We analyzed seven deleted genes, including MGF genes, from the right variable region of the ASFV genome against sera from convalescent pigs. MGF100-1L showed significant reactivity and was produced as a recombinant protein for use in an enzyme-linked immunosorbent assay (ELISA). The assay, with a cut-off value of 0.284, successfully differentiated between naive and infected pigs with 100% accuracy. More importantly, pigs infected with the cell-adapted ASFV showed no significant change in ELISA readouts after 27 days post-infection. However, when these pigs were subsequently challenged with wild-type virus, MGF100-1L reactivity increased significantly by 21 days post-challenge. This study demonstrates the potential of MGF100-1L as a DIVA marker for ASFV, which offers a promising tool to distinguish between infections with wild-type ASFV and those with cell-adapted variants lacking specific MGF genes, thereby improving ASFV surveillance and control strategies.","PeriodicalId":94266,"journal":{"name":"Virology","volume":"603 ","pages":"110349"},"PeriodicalIF":0.0,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142831488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative RNA sequencing analysis of three Capripoxvirus infections in an immortalized hTERT-bOEC cell model. 永生化hTERT-bOEC细胞模型中三种Capripoxvirus感染的比较RNA测序分析。

Virology Pub Date : 2024-12-14 DOI: 10.1016/j.virol.2024.110352

Hongqiang Zhang, Fangping Wang, Haotai Chen, Shasha Wang, Lina Tong, Huibao Wang, Jiangfeng Fan, Xiangping Yin, Xiangwei Wang, Yuefeng Sun, Xiaolong Gao, Shanhui Ren

{"title":"Comparative RNA sequencing analysis of three Capripoxvirus infections in an immortalized hTERT-bOEC cell model.","authors":"Hongqiang Zhang, Fangping Wang, Haotai Chen, Shasha Wang, Lina Tong, Huibao Wang, Jiangfeng Fan, Xiangping Yin, Xiangwei Wang, Yuefeng Sun, Xiaolong Gao, Shanhui Ren","doi":"10.1016/j.virol.2024.110352","DOIUrl":"https://doi.org/10.1016/j.virol.2024.110352","url":null,"abstract":"Capripoxviruses (CaPVs), such as lumpy skin disease, sheep pox, and goat pox, cause significant production and economic losses and are major constraints to the growth of livestock production in endemic areas. Understanding the pathogenic mechanism of CaPVs and their translation into clinical applications depends on the availability of a suitable cell line. In this study, we used a lentiviral packaging system to establish an immortalized hTERT-bOEC cell line by ectopic introduction of human telomerase reverse transcriptase (hTERT). Western blotting, indirect immunofluorescence, and flow cytometry analyses revealed that hTERT was successfully integrated into the genome of hTERT-bOEC cells. Crucially, this hTERT-bOEC cell line was highly susceptible to LSDV, SPPV, and GTPV infections. Establishing hTERT-bOECs is critical for basic research, clinical application, and vaccine development related to CaPVs. Furthermore, RNA-seq analyses revealed a similar differential expression of genes and enrichment of signaling pathways to CaPV infections in hTERT-bOECs. Real-time quantitative qPCR further confirmed the top five up-regulated and down-regulated differentially expressed genes among the CaPV infections. Transcriptome analyses provide deep insight into the biological characteristics of the replication process in CaPV infections.","PeriodicalId":94266,"journal":{"name":"Virology","volume":"603 ","pages":"110352"},"PeriodicalIF":0.0,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142866955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of African swine fever virus antibodies using p11.5 and p14.5 protein-based indirect ELISA. 基于p11.5和p14.5蛋白的间接ELISA检测非洲猪瘟病毒抗体。

Virology Pub Date : 2024-12-13 DOI: 10.1016/j.virol.2024.110335

Aiping Wang, Hehe Zhao, Hongliang Liu, Yumei Chen, Jingming Zhou, Xifang Zhu, Chao Liang, Peiyang Ding, Enping Liu, Gaiping Zhang

引用次数: 0

Distinct genomic features of Transeurasian strains of Epstein-Barr virus in East Asia. 东亚跨欧亚爱泼斯坦-巴氏病毒毒株的基因组特征。

Virology Pub Date : 2024-12-12 DOI: 10.1016/j.virol.2024.110359

Hiroshi Kitamura, Iwao Kukimoto, Misako Yajima, Kazufumi Ikuta, Kenroh Sasaki, Teru Kanda

引用次数: 0

Development of RNA reference materials for norovirus GI and GII using digital PCR. 利用数字PCR技术制备诺如病毒GI和GII的RNA对照物。

Virology Pub Date : 2024-12-11 DOI: 10.1016/j.virol.2024.110358

Da-Hye Lee, Hyo Jung Ju, Yoojin Lee, Young-Kyung Bae

引用次数: 0

Editorial: Viruses and cancer. 社论：病毒和癌症。

Virology Pub Date : 2024-12-09 DOI: 10.1016/j.virol.2024.110353

Takayuki Murata, Yoshihiro Izumiya

引用次数: 0

Previously unreported Arg594Lys in EBNA-1 and Leu212 in EBNA-2 among patients with EBV-associated infectious mononucleosis in Croatia. 克罗地亚ebv相关传染性单核细胞增多症患者EBNA-1中的Arg594Lys和EBNA-2中的Leu212先前未报道。

Virology Pub Date : 2024-12-07 DOI: 10.1016/j.virol.2024.110340

Marija Rozman, Laura Prtorić, Ante Šokota, Kristian Bodulić, Goran Tešović, Snjezana Zidovec-Lepej

{"title":"Previously unreported Arg594Lys in EBNA-1 and Leu212 in EBNA-2 among patients with EBV-associated infectious mononucleosis in Croatia.","authors":"Marija Rozman, Laura Prtorić, Ante Šokota, Kristian Bodulić, Goran Tešović, Snjezana Zidovec-Lepej","doi":"10.1016/j.virol.2024.110340","DOIUrl":"https://doi.org/10.1016/j.virol.2024.110340","url":null,"abstract":"The molecular diversity of Epstein-Barr virus (EBV) is defined by mutations in specific EBV genes and has been insufficiently studied in infectious mononucleosis (IM). The aim of this study was to determine all variations of the EBV latency genes EBNA-1, EBNA-2 and LMP-1 in pediatric patients with EBV-associated IM in Croatia, including previously defined SNPs and indels as well as previously undocumented polymorphisms. The vast majority of EBV isolates (71/72) were determined as EBV type 1 while EBNA-1 genes were classified exclusively as previously defined EBNA-1 prototypes, with 22/72 sequences categorized as P-Ala and 50/72 sequences as P-Thr. The most common LMP-1 variants included wild type (B95-8, 20/72), China1 (19/72) and recombinants (10/72). This study also described a previously undocumented polymorphism in the Arg594Lys substitution that is present in all EBNA-1 sequences examined. In addition, we found a Leu212 insertion in the EBNA-2 sequences of 50/72 isolates compared to the wild type. These polymorphisms were described for the first time in this geographic region and were not mentioned in previous studies on EBV diversity in IM. We also concluded mutual variant association between the variants using a chi-square test, in which the LMP-1 North Carolina variant was significantly more likely to appear with the EBNA-1 P-Ala prototype, while the B95-8 LMP-1 variant was significantly more likely to appear with the EBNA-1 P-Thr prototype (p < 0.05). Furthermore, leucine addition in EBNA-2 sequences is more likely to appear with LMP-1 wild type and EBNA-1 P-Thr prototype while EBV type 1 identical to the reference sequence is more likely to appear with North Carolina LMP-1 variant and EBNA-1 P-Ala prototype (p < 0.05).","PeriodicalId":94266,"journal":{"name":"Virology","volume":"603 ","pages":"110340"},"PeriodicalIF":0.0,"publicationDate":"2024-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142796751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0