{"title":"Detection of African swine fever virus antibodies using p11.5 and p14.5 protein-based indirect ELISA.","authors":"Aiping Wang, Hehe Zhao, Hongliang Liu, Yumei Chen, Jingming Zhou, Xifang Zhu, Chao Liang, Peiyang Ding, Enping Liu, Gaiping Zhang","doi":"10.1016/j.virol.2024.110335","DOIUrl":null,"url":null,"abstract":"<p><p>African swine fever (ASF), which is caused by the African swine fever virus (ASFV). There are currently no effective vaccines or therapies for ASF; rapid diagnosis has become an important way to control the disease. The p11.5 and p14.5 proteins are structural proteins of ASFV that have immunogenicity and potential as diagnostic antigens. In this study, p11.5 and p14.5 proteins were used as diagnostic antigens in an indirect ELISA examine to detect ASFV antibodies. The p11.5 and p14.5-iELISA methods showed no cross-reaction with other swine virus positive sera. The lowest detection limit of positive serum was 1: 6400, and the coefficients of variation (CV) of intra - and inter-assay were less than 7%. By comparing 71 serum samples, the coincidence rate of p11.5 and p14.5-iELISA with commercial ELISA kits was 91.55%. Taken together, these results showed that there is a reliable method of detecting ASFV antibodies in clinical pig serum.</p>","PeriodicalId":94266,"journal":{"name":"Virology","volume":"603 ","pages":"110335"},"PeriodicalIF":0.0000,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Virology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1016/j.virol.2024.110335","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
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Abstract
African swine fever (ASF), which is caused by the African swine fever virus (ASFV). There are currently no effective vaccines or therapies for ASF; rapid diagnosis has become an important way to control the disease. The p11.5 and p14.5 proteins are structural proteins of ASFV that have immunogenicity and potential as diagnostic antigens. In this study, p11.5 and p14.5 proteins were used as diagnostic antigens in an indirect ELISA examine to detect ASFV antibodies. The p11.5 and p14.5-iELISA methods showed no cross-reaction with other swine virus positive sera. The lowest detection limit of positive serum was 1: 6400, and the coefficients of variation (CV) of intra - and inter-assay were less than 7%. By comparing 71 serum samples, the coincidence rate of p11.5 and p14.5-iELISA with commercial ELISA kits was 91.55%. Taken together, these results showed that there is a reliable method of detecting ASFV antibodies in clinical pig serum.
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