The protein journal最新文献

Utilisation of Carbon Quantum Dots from Hazelnut Husk for Folic Acid (FA) Detection: An Innovative Approach.

The protein journal Pub Date : 2025-03-08 DOI: 10.1007/s10930-025-10249-w

Ali Arda Ciritcioğlu, Erdem Elibol, Zehra Günaydın, Tuna Demirci

{"title":"Utilisation of Carbon Quantum Dots from Hazelnut Husk for Folic Acid (FA) Detection: An Innovative Approach.","authors":"Ali Arda Ciritcioğlu, Erdem Elibol, Zehra Günaydın, Tuna Demirci","doi":"10.1007/s10930-025-10249-w","DOIUrl":"10.1007/s10930-025-10249-w","url":null,"abstract":"This study presents the development of a carbon quantum dot (CQD)-based fluorescence sensor for the accurate quantification of Folic Acid (FA). CQDs were synthesized from hazelnut husk using a solvothermal method and functionalized with silver ions to create an \"off-state\" fluorescence system. Upon mixing FA solutions, prepared from pure water and pharmaceutical tablets, with phosphate-buffered saline (PBS) and \"off-state\" CQDs, fluorescence emission was restored (\"on-state\") in a concentration-dependent manner when excited at 360 nm. A strong linear relationship was observed between FA concentration and fluorescence intensity, with an R² value of ≈ 0.994. The samples were categorized into low (0.0376-0.7533 µM) and high (0.7533-7.533 µM) concentration groups for improved accuracy, achieving mean percentage errors of 0.70% and 1.85%, respectively, at concentrations as low as 0.565 µM. This CQD-based sensor demonstrated rapid, cost-effective, and highly sensitive detection capabilities, making it a promising alternative for FA quantification in biomedical and nutritional applications. Furthermore, the use of sustainable raw materials, such as hazelnut husk, highlights the eco-friendly and practical advantages of this method over conventional techniques.","PeriodicalId":94249,"journal":{"name":"The protein journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143582388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Phosphodiesterase Type-5 (PDE-5) Inhibitor, Sildenafil, Ameliorates the NEC Induced Inflammation.

The protein journal Pub Date : 2025-03-08 DOI: 10.1007/s10930-025-10263-y

Mehmet Akif Ovalı, Özlem Öztopuz, İhsan Karaboğa

{"title":"A Phosphodiesterase Type-5 (PDE-5) Inhibitor, Sildenafil, Ameliorates the NEC Induced Inflammation.","authors":"Mehmet Akif Ovalı, Özlem Öztopuz, İhsan Karaboğa","doi":"10.1007/s10930-025-10263-y","DOIUrl":"10.1007/s10930-025-10263-y","url":null,"abstract":"The connection between intestine microbiota and lung disease is described as the gut-lung axis, these organ systems are somehow interrelated in both homeostasis and disease development. In newborns, the most important gastrointestinal complications are necrotizing enterocolitis (NEC), and the pulmonary complication both cause significant systemic morbidity. In this study, sildenafil administered at varying doses in neonatal rat model of experimental necrotizing enterocolitis and focused on both mRNA expression and histopathological alterations. 15-day-old Wistar Albino rat pups were randomly divided into six groups; Control, NEC, DMSO, Sil_1mg, Sil_5mg, Sil_10mg (n = 5). NEC induction was performed using hypoxia/asphyxia and cold stress. At the end of the experiment, lung tissues were harvested, molecular and histopathological alterations were analysed. Histopathological examination was performed with hematoxylin&eosin and masson trichrome staining in lung samples of neonatal rats and the mRNA expression levels of TNF-α, IL-6 and HSPa5 genes were analyzed. The mRNA expression levels of TNF-α, IL-6 and HSPa5 were increased in the NEC group compared to the control group and sildenafil treatment could significantly reduced the levels of the genes and inflammation (*p < 0.05 and **p ≤ 0.0001). Alveolar edema and hemorrhage findings were observed in the lung tissue of the NEC group. Interstitial edema and hemorrhage findings were reduced in the groups treated with sildenafil compared to the NEC group. The data we obtained indicate that sildenafil administering at different doses has therapeutic effect on NEC induced lung tissue inflammation both at the mRNA expression and tissue levels.","PeriodicalId":94249,"journal":{"name":"The protein journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143582392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibitor Action of Unsaturated Fatty Acids on Equine Serum Butyrylcholinesterase.

The protein journal Pub Date : 2025-02-22 DOI: 10.1007/s10930-025-10259-8

Mehmet Berk Akay, Kubra Sener, Suat Sari, Ebru Bodur

{"title":"Inhibitor Action of Unsaturated Fatty Acids on Equine Serum Butyrylcholinesterase.","authors":"Mehmet Berk Akay, Kubra Sener, Suat Sari, Ebru Bodur","doi":"10.1007/s10930-025-10259-8","DOIUrl":"https://doi.org/10.1007/s10930-025-10259-8","url":null,"abstract":"Butyrylcholinesterase (BChE; EC 3.1.1.8), a serine hydrolase found in various tissues, hydrolyses choline esters such as acetylcholine and succinylcholine, as well as other esters such as heroin and acetylsalicylic acid. It is considered to play a role in lipid metabolism as it belongs to the same enzyme group as lipases and its catalytic subunits are similar. In this study, the effects of unsaturated fatty acids, namely arachidonic (AA), linoleic (LA), alpha-linolenic (ALA) and oleic acid (OA), on equine serum BChE (EqBChE) were investigated. Enzyme activity was measured by the modified Ellman method. When the activity results were evaluated, the IC50 values were found 45.49, 8.465, 1556, and 56.57 μM; while the Ki values were 63.92, 11.46, 1800, and 15.24 μM for AA, ALA, LA, and OA, respectively. Analysis of the kinetic results showed that ALA was compatible with mixed inhibition and other fatty acids were compatible with non-competitive inhibition, a special type of mixed inhibition. Molecular docking predicted binding of the fatty acids to the active site, as well as to predicted allosteric sites. The results of this study provide another support to the hypothesis that cholinesterases are associated with lipid metabolism.","PeriodicalId":94249,"journal":{"name":"The protein journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143476520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Heavy Metal Stress on Seedling Growth and Antioxidant System in Sorghum (Sorghum Bicolor (L.) Moench).

The protein journal Pub Date : 2025-02-22 DOI: 10.1007/s10930-025-10258-9

Büşra Çevık, Hakan Arslan, Deniz Ekinci

{"title":"Effects of Heavy Metal Stress on Seedling Growth and Antioxidant System in Sorghum (Sorghum Bicolor (L.) Moench).","authors":"Büşra Çevık, Hakan Arslan, Deniz Ekinci","doi":"10.1007/s10930-025-10258-9","DOIUrl":"https://doi.org/10.1007/s10930-025-10258-9","url":null,"abstract":"Factors that impede the normal growth and development of plants are termed 'stress factors' and result in yield loss. Exposure to elevated concentrations of heavy metals has been demonstrated to induce the production of reactive oxygen species (ROS), which can affect physiological and biochemical processes. The present study investigated the effects of two common heavy metals (Hg2+ and Cd2+) on specific physiological and biochemical parameters of sorghum (Sorghum bicolor (L.) Moench). The study utilized doses ranging from 3 to 15 ppm for the application of heavy metals. The study focused on the effects of these metals on the activities of enzymes (superoxide dismutase and peroxidase), chlorophyll content, fresh weight, dry weight, and proline content in sorghum plants. The study found that applying mercury at 9 ppm resulted in the highest activity of peroxidase (POX), with an observed increase of 69.57% compared to the control. Conversely, cadmium application at 12 ppm elicited the highest activity, increasing by 102.17% compared to the control. For superoxide dismutase (SOD), the peak activity was observed at 6 ppm for both applications, with an increase of 84.16% in the mercury application control group and 121.08% in the cadmium application group compared to the control group. A similar pattern was observed in the chlorophyll content, which initially increased and then decreased. Declines in fresh weight, dry weight, and proline accumulation were also documented.","PeriodicalId":94249,"journal":{"name":"The protein journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143476552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Low-dose Bee Venom as a Potential Therapeutic Agent Against Human Chronic Myeloid Leukaemia Cells.

The protein journal Pub Date : 2025-02-09 DOI: 10.1007/s10930-025-10251-2

Hamza Halici, Harun Un, Saffet Celik, Zeynep Karakoy, Zafer Bayraktutan, Can Ozlu, Elif Cadirci, Zekai Halici, Alptug Atila, Filiz Mercantepe

{"title":"Low-dose Bee Venom as a Potential Therapeutic Agent Against Human Chronic Myeloid Leukaemia Cells.","authors":"Hamza Halici, Harun Un, Saffet Celik, Zeynep Karakoy, Zafer Bayraktutan, Can Ozlu, Elif Cadirci, Zekai Halici, Alptug Atila, Filiz Mercantepe","doi":"10.1007/s10930-025-10251-2","DOIUrl":"https://doi.org/10.1007/s10930-025-10251-2","url":null,"abstract":"Bee venom is secreted by a gland in the abdominal cavity of bees. The venom, especially that of honeybees, contains certain enzymes and peptides that, when administered in high doses, are effective against various diseases. Peptides such as melittin and phospholipase A2 can target various cancer cells. In this study, we investigated the antiproliferative effects of administering low-dose bee venom in K-562 chronic myeloid leukaemia cells. Our proteomic study revealed regional variation of the content of bee venom and high levels of melittin, apamin and secapin, as well as phospholipase A2 and hyaluronidase. In addition, eight new, previously unidentified proteins were identified. The effects of bee venom on cell viability and drug-cell interaction were investigated at 24, 48 and 72 h. According to the MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) results, the bee venom decreased K-562 cell viability dose-dependently at all time points. Cell viability decreased 48 and 72 h after bee venom administration but increased in the control group left untreated for 72 h. The inhibition percentages for the highest bee venom concentration (0.4 µM) at 24, 48 and 72 h were 55%, 80% and 92%, respectively. The cell-drug interactions indicated that the cell surfaces, which were smooth and clear before drug application, gradually deteriorated and started to shrink after the application. In conclusion, at increasing doses, bee venom was found to have a strong antiproliferative effect in K-562 chronic myeloid leukaemia cell lines.","PeriodicalId":94249,"journal":{"name":"The protein journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143384625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effect of Inhaled Ozone Therapy in Two-Hit Rat Model of Lipopolysaccharides-Induced Acute Lung Injury and Bleomycin-Induced Pulmonary Fibrosis.

The protein journal Pub Date : 2025-02-07 DOI: 10.1007/s10930-024-10247-4

Mine Celik, Mehmet Koca, Zekai Halici, Taha Tavaci, Hamza Halici, Mustafa Ozkaraca, Zeynep Karakoy, Zafer Bayraktutan

{"title":"The Effect of Inhaled Ozone Therapy in Two-Hit Rat Model of Lipopolysaccharides-Induced Acute Lung Injury and Bleomycin-Induced Pulmonary Fibrosis.","authors":"Mine Celik, Mehmet Koca, Zekai Halici, Taha Tavaci, Hamza Halici, Mustafa Ozkaraca, Zeynep Karakoy, Zafer Bayraktutan","doi":"10.1007/s10930-024-10247-4","DOIUrl":"https://doi.org/10.1007/s10930-024-10247-4","url":null,"abstract":"Considering the limited treatment options for acute lung injury (ALI) and pulmonary fibrosis (PF), ozone treatment may be promising as a new immunological agent with its ability to modulate cytokines and interferons. We aimed to investigate the effects of inhaled ozone therapy on both ALI and PF in rat models. A total of 48 albino Wistar male rats were included in the study. Lipopolysaccharide (LPS) was used to induce the ALI model, and bleomycin was used for the PF model. The effects of inhaled ozone (O3) were investigated using the ELISA method. Hematoxylin&eosin staining, Masson's trichrome staining, and immunohistochemical methods were used for histopathological evaluation. The Interleukin-1 beta (IL-1β), Interleukin-6 (IL-6), Tumor Necrosis Factor-alpha (TNF-α), and Nuclear Factor kappa B subunit p65 (NF-κB p65) levels in the ALI + 0.08 ppm O3, ALI + 0.12 ppm O3, PF + 0.08 ppm O3, and PF + 0.12 ppm O3 groups statistically decreased to the same extent and approached the levels of control animals. It was observed that IL-1β, IL-6, TNF-α, and NF-κB p65 levels in lung tissues were significantly and dose-dependently decreased compared to the untreated PF and ALI groups, respectively. While fibrosis was severe in the PF + 0.08 ppm O3 group, it decreased to more moderate levels in the PF + 0.12 ppm O3 group. The cytokine levels confirmed that inhaled ozone protected the lungs from both ALI and the development of PF.","PeriodicalId":94249,"journal":{"name":"The protein journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143371416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From Crude Extracts to Purity: A Comparative Study of Butyrylcholinesterase Purification.

The protein journal Pub Date : 2025-02-07 DOI: 10.1007/s10930-025-10248-x

Gamze Sonmez, Bahattin Enes Karatas, Ebru Bodur

引用次数: 0

Expression of Recombinant Stonustoxin Alpha Subunit and Preparation of polyclonal antiserum for Stonustoxin Neutralization Studies. 重组石蒜毒素α亚基的表达和用于石蒜毒素中和研究的多克隆抗血清的制备。

The protein journal Pub Date : 2024-06-01 Epub Date: 2024-05-17 DOI: 10.1007/s10930-024-10203-2

Amir Sajjad Hojjati-Razgi, Shahram Nazarian, Hossein Samiei-Abianeh, Amir Vazirizadeh, Emad Kordbacheh, Seyed Mojtaba Aghaie

{"title":"Expression of Recombinant Stonustoxin Alpha Subunit and Preparation of polyclonal antiserum for Stonustoxin Neutralization Studies.","authors":"Amir Sajjad Hojjati-Razgi, Shahram Nazarian, Hossein Samiei-Abianeh, Amir Vazirizadeh, Emad Kordbacheh, Seyed Mojtaba Aghaie","doi":"10.1007/s10930-024-10203-2","DOIUrl":"10.1007/s10930-024-10203-2","url":null,"abstract":"Stonustoxin (SNTX) is a lethal protein found in stonefish venom, responsible for many of the symptoms associated with stonefish envenomation. To counter stonefish venom challenges, antivenom is a well-established and effective solution. In this study, we aimed to produce the recombinant alpha subunit protein of Stonustoxin from Synanceia horrida and prepare antibodies against it The SNTXα gene sequence was optimized for E. coli BL21 (DE3) expression and cloned into the pET17b vector. Following purification, the recombinant protein was subcutaneously injected into rabbits, and antibodies were extracted from rabbit´s serum using a G protein column As a result of codon optimization, the codon adaptation index for the SNTXα cassette increased to 0.94. SDS-PAGE analysis validated the expression of SNTXα, with a band observed at 73.5 kDa with a yield of 60 mg/l. ELISA results demonstrated rabbits antibody titers were detectable up to a 1:256,000 dilution. The isolated antibody from rabbit´s serum exhibited a concentration of 1.5 mg/ml, and its sensitivity allowed the detection of a minimum protein concentration of 9.7 ng. In the neutralization assay the purified antibody against SNTXα protected mice challenged with 2 LD50. In conclusion, our study successfully expressed the alpha subunit of Stonustoxin in a prokaryotic host, enabling the production of antibodies for potential use in developing stonefish antivenom.","PeriodicalId":94249,"journal":{"name":"The protein journal","volume":" ","pages":"627-638"},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140961268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0