The international journal of lower extremity wounds最新文献_第6页

Antimicrobial Peptide Buforin-I Facilitates the Healing of Foot Wounds in Diabetic Mice. 抗菌肽buforin - 1促进糖尿病小鼠足部伤口愈合。

The international journal of lower extremity wounds Pub Date : 2025-05-30 DOI: 10.1177/15347346251336569

Huang Hu, Hong Zhang, Meixian He, Jingjing Tai

{"title":"Antimicrobial Peptide Buforin-I Facilitates the Healing of Foot Wounds in Diabetic Mice.","authors":"Huang Hu, Hong Zhang, Meixian He, Jingjing Tai","doi":"10.1177/15347346251336569","DOIUrl":"https://doi.org/10.1177/15347346251336569","url":null,"abstract":"BackgroundDiabetic wounds are difficult to heal due to long-term chronic ulcers, thus lacking effective treatment methods. Some antimicrobial peptides are known to have biological functions that promote wound healing, but the effects of the antimicrobial peptide Buforin-I on diabetic wound healing and the mechanisms underlying these effects are not yet elucidated.MethodsThis project investigated the effects of Buforin-I on the healing rate of foot wounds in streptozotocin (STZ)-induced diabetic mice and the antioxidant reactions in wound tissues. Histological staining with HE and Masson, as well as biochemical assays with immunohistochemistry (IHC), were applied to assess tissue chemistry markers. The activity levels of SOD, GSH, and reactive oxygen species (ROS) were measured to evaluate oxidative stress levels. TUNEL analysis was carried out to detect the apoptosis rate. The levels of VEGF, TGF-β1, and Nrf2 were measured by enzyme-linked immunosorbent assay (ELISA) and western blot (WB). In addition, the in vitro effects of Buforin-I on cell proliferation, apoptosis, migration, and oxidative stress levels under high glucose conditions were analyzed on HaCaT cells. Cell viability was measured using an MTT assay, and cell migration was assessed through the wound healing experiment.ResultsBuforin-I treatment accelerated the healing of foot wounds in STZ-induced diabetic mice, with increased fibroblast proliferation and collagen deposition in the wound tissue and decreased Nrf2-mediated oxidative stress levels. Furthermore, Buforin-I facilitated the proliferation and migration as well as reduced apoptosis of HaCaT cells under high glucose conditions, thus enhancing the antioxidant capacity of HaCaT cells.ConclusionIn conclusion, this study confirmed the promoting effect of Buforin-I on diabetic wound healing through the regulation of oxidative stress, highlighting the potential application of Buforin-I in diabetic wound healing.","PeriodicalId":94229,"journal":{"name":"The international journal of lower extremity wounds","volume":" ","pages":"15347346251336569"},"PeriodicalIF":0.0,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144192633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlation of Wound Prognosis with serum IL-6, ICAM-1 and sST2 in Patients with Diabetic Foot and Construction of a Nomogram Model. 糖尿病足患者伤口预后与血清IL-6、ICAM-1、sST2的相关性及Nomogram模型的建立

The international journal of lower extremity wounds Pub Date : 2025-05-30 DOI: 10.1177/15347346251345262

Yali Zhou, Wenhu Zhou, Yu Guo, Changping Hu

{"title":"Correlation of Wound Prognosis with serum IL-6, ICAM-1 and sST2 in Patients with Diabetic Foot and Construction of a Nomogram Model.","authors":"Yali Zhou, Wenhu Zhou, Yu Guo, Changping Hu","doi":"10.1177/15347346251345262","DOIUrl":"https://doi.org/10.1177/15347346251345262","url":null,"abstract":"Diabetes foot (DF) is one of the most serious chronic complications of diabetes. This study explored the relationship between serum interleukin-6 (IL-6), intercellular adhesion molecule-1 (ICAM-1) and soluble suppression of tumorigenicity 2 (sST2) and wound prognosis in 210 DF patients between January 2019 and January 2024. 210 DF patients were divided into the good prognosis (n = 147) and poor prognosis (n = 63) group according to the prognosis. Comparative analysis revealed that levels of serum IL-6, ICAM-1 and sST2 in the poor prognosis group were all higher than those in the good prognosis group significantly (P < .05). Multivariate logistic regression identified these 3 biomarkers as independent risk factors for poor wound healing (P < .05). Positive correlations between serum IL-6 (r = 0.269), ICAM-1 (r = 0.302), sST2 (r = 0.289) levels and poor prognosis were confirmed through Pearson's correlation analysis. A prediction model was established to analyse their predictive value. The training and validation sets ROC curves had AUCs of 0.79 (0.71-0.87) and 0.75 (0.59-0.91) respectively. Calibration curves were plotted to evaluate the consistency of the model, and the results showed that the predictive value of the nomogram model was similar to that of the actual one. Decision curves were plotted, which showed that the nomogram had higher positive net benefit in the range of 20% to 60%. This study suggest that serum IL-6, ICAM-1, and sST2 levels may serve as valuable prognostic indicators for wound healing progression in DF patients, with combined biomarker assessment showing potential clinical utility for outcome prediction. The total sample size (n = 210), with validation set (n = 63) of this study are relatively limited and the representativeness is restricted, which may affect the universality of the research conclusions.","PeriodicalId":94229,"journal":{"name":"The international journal of lower extremity wounds","volume":" ","pages":"15347346251345262"},"PeriodicalIF":0.0,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144192634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel Tissue-Engineered Amniotic Dressing for Chronic Wound Healing. 用于慢性伤口愈合的新型组织工程羊膜敷料。

The international journal of lower extremity wounds Pub Date : 2025-05-29 DOI: 10.1177/15347346241308087

Pengwen Ni, Kefeng Zhang, Xin Wu, Zhaoqi Jiang, Yao Huang, Beiqian Mao, Bowen Liu, Ting Xie

引用次数: 0

Advancements in Deep Wounds Therapies: The Synergistic Application of Laser Therapy and Nanomaterials. 深度伤口治疗的进展：激光治疗和纳米材料的协同应用。

The international journal of lower extremity wounds Pub Date : 2025-05-27 DOI: 10.1177/15347346251345255

Zahra Al Timimi, Diwan Mohsen

引用次数: 0

Jean-Martin Charcot: 200 years after his birth, still a paragon in the diabetic foot. Jean-Martin Charcot：在他出生200年后，仍然是糖尿病足的典范。

The international journal of lower extremity wounds Pub Date : 2025-05-25 DOI: 10.1177/15347346251343915

Nina L Petrova, Michael E Edmonds, Nikolaos Papanas

引用次数: 0

Efficacy of Topical Timolol in Chronic Unhealed Ulcers: A Systematic Review and Meta-Analysis. 局部替莫洛尔治疗慢性未愈合溃疡的疗效：一项系统综述和荟萃分析。

The international journal of lower extremity wounds Pub Date : 2025-05-23 DOI: 10.1177/15347346251345249

Muhammad M Elsharkawy, Eslam Mohammed Rabea, Anas Ashraf Elassal, Anan Maher Salaimeh, Adam Naji Zaro, Saad Mohamed Saad, Mohamed Eldesouki

{"title":"Efficacy of Topical Timolol in Chronic Unhealed Ulcers: A Systematic Review and Meta-Analysis.","authors":"Muhammad M Elsharkawy, Eslam Mohammed Rabea, Anas Ashraf Elassal, Anan Maher Salaimeh, Adam Naji Zaro, Saad Mohamed Saad, Mohamed Eldesouki","doi":"10.1177/15347346251345249","DOIUrl":"https://doi.org/10.1177/15347346251345249","url":null,"abstract":"BackgroundTopical timolol appears to provide a curative effect in managing chronic ulcers. However, the pooled evidence of its effect hasn't been established yet. This is the first Meta-analysis to evaluate and assess the efficacy of topical timolol in accelerating wound healing in patients with chronic refractory ulcers.MethodsWe comprehensively searched different electronic databases; PubMed, Scopus, Web of Science, Cochrane library and ClinicalTrials.gov from their inception to November 2024. The search was conducted without imposing any restrictions on publication date or study design. The ROB-II tool was used to assess the quality of included RCTs. NIH tools were used to evaluate the quality of included cohort and single arm studies. Risk ratios (RR) and mean differences (MD), with 95% confidence intervals (CIs), were used as effect estimates.ResultsSix studies were included in this systematic review, comprising two single-arm studies and four double-arm studies (3 RCTs and 1 cohort study). Data from the four double-arm studies on venous ulcers were pooled for meta-analysis. The results showed that topical timolol significantly reduced ulcer area compared to control at 2 weeks (-23.63%; 95% CI: -29.26 to -18.00; p < 0.00001), 4 weeks (-41.40%; 95% CI: -49.57 to -33.23; p < 0.00001), and 12 weeks (-23.38%; 95% CI: -39.43 to -7.33; p = 0.004). Complete ulcer healing was observed at 4 weeks (RR: 12.00; 95% CI: 1.68 to 85.84; p = 0.01) and 12 weeks (RR: 3.79; 95% CI: 0.44 to 32.54; p = 0.22).ConclusionTopical timolol has the potential to promote healing in chronic unhealed ulcers. This meta-analysis, focusing on chronic venous ulcers, consistently showed favorable results across different time points. However, the small sample sizes, lack of blinding, and inclusion of only four studies limit the generalizability of the findings.","PeriodicalId":94229,"journal":{"name":"The international journal of lower extremity wounds","volume":" ","pages":"15347346251345249"},"PeriodicalIF":0.0,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144129802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chronic Kidney Disease as an Independent Risk Factor for Chronic Lower Extremity Ulcers: A Cross-Sectional Analysis of US Adults. 慢性肾脏疾病是慢性下肢溃疡的独立危险因素：美国成年人的横断面分析

The international journal of lower extremity wounds Pub Date : 2025-05-22 DOI: 10.1177/15347346251343707

Hai Gao, Juanmin Yang, Zhaohui Wang, Shu Wu, Yangyan Yi

{"title":"Chronic Kidney Disease as an Independent Risk Factor for Chronic Lower Extremity Ulcers: A Cross-Sectional Analysis of US Adults.","authors":"Hai Gao, Juanmin Yang, Zhaohui Wang, Shu Wu, Yangyan Yi","doi":"10.1177/15347346251343707","DOIUrl":"https://doi.org/10.1177/15347346251343707","url":null,"abstract":"BackgroundChronic lower extremity ulcers (CLEU) are a significant health burden, often linked to complications such as diabetes and vascular diseases. Chronic kidney disease (CKD), a prevalent global health issue, has been increasingly associated with CLEU, though the nature of this relationship remains poorly understood. This study aimed to explore the association between CKD and CLEU in the US adult population.MethodsWe analyzed data from the National Health and Nutrition Examination Survey (NHANES) between 1999 and 2004. The study included adults aged 20 years and older who completed interviews and examinations. CLEU was defined as self-reported ulcers or sores on the leg or foot lasting more than four weeks. CKD was diagnosed based on the 2012 KDIGO guidelines, using estimated glomerular filtration rate (eGFR) and urinary albumin-to-creatinine ratio (UACR). Logistic regression models were used to assess the association between CKD and CLEU, adjusting for demographics, BMI, lifestyle factors, and comorbidities.ResultsAmong 8564 participants, individuals with CKD had a higher likelihood of developing CLEU, with an unadjusted odds ratio (OR) of 2.33 (95% CI: 1.86-2.91). After adjusting for confounders, the association remained significant, with adjusted ORs of 1.64 (95% CI: 1.26-2.12) and 1.57 (95% CI: 1.2-2.04) in Models 2 and 3, respectively. Stratified analyses showed no significant interactions across subgroups defined by age, sex, diabetes, hypertension, hyperlipidemia, and peripheral artery disease (PAD).ConclusionThis study demonstrates a robust association between CKD and CLEU in US adults, suggesting that CKD is an independent risk factor for CLEU. These findings highlight the need for integrated management strategies for CKD patients to reduce the risk of CLEU. Future longitudinal studies are needed to establish causality and inform targeted interventions.","PeriodicalId":94229,"journal":{"name":"The international journal of lower extremity wounds","volume":" ","pages":"15347346251343707"},"PeriodicalIF":0.0,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144121839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Authorship Disputes: An Endemic Plague. 作者争议：一场地方性瘟疫。

The international journal of lower extremity wounds Pub Date : 2025-05-14 DOI: 10.1177/15347346251341635

Miltos K Lazarides, Nikolaos Papanas

引用次数: 0

Higher Serum Levels of Lipoprotein-Associated Phospholipase A2 are Associated with Diabetic Foot Osteomyelitis. 血清脂蛋白相关磷脂酶A2水平升高与糖尿病足骨髓炎有关

The international journal of lower extremity wounds Pub Date : 2025-05-13 DOI: 10.1177/15347346251340757

Haojie Sun, Shucai Zhang, Xuekui Liu, Houfa Geng, Jun Liang

{"title":"Higher Serum Levels of Lipoprotein-Associated Phospholipase A2 are Associated with Diabetic Foot Osteomyelitis.","authors":"Haojie Sun, Shucai Zhang, Xuekui Liu, Houfa Geng, Jun Liang","doi":"10.1177/15347346251340757","DOIUrl":"https://doi.org/10.1177/15347346251340757","url":null,"abstract":"BackgroundDiabetic foot osteomyelitis (DFO) is often difficult to detect in its early stages, making diagnosis challenging. This study is to investigate the relationship between lipoprotein-associated phospholipase A2 (Lp-PLA2) and DFO, while also evaluating the potential of Lp-PLA2 as an early diagnostic biomarker for DFO.MethodsA total of 265 patients with type 2 diabetes complicated by diabetic foot infections were selected for the study. Participants were divided into the Non-DFO group (184 cases) and the DFO group (81 cases). Serum Lp-PLA2 levels were measured. Logistic regression analysis was utilized to identify risk factors associated with DFO. Receiver Operating Characteristic curve analysis was performed to assess the diagnostic value of Lp-PLA2 in patients with DFO.ResultsLp-PLA2 in the DFO group were significantly higher than those in the Non-DFO group (P < .05). Lp-PLA2 (OR, 1.015 [95% CI, 1.011-1.019]; P < .001), lower extremity arterial disease (OR, 2.453 [95% CI, 1.147-5.247]; P < .05), and ESR (OR, 1.042 [95% CI, 1.026-1.059]; P < .001) were the independent risk factors for DFO. Lp-PLA2 and ESR were shown to be associated with DFO, with their respective areas under the curve (AUC) being 0.787 and 0.683. The sensitivity and specificity of Lp-PLA2 were 65.4% and 82.6%, respectively, while those of ESR were 48.1% and 95.1%. Combined Lp-PLA2 and ESR showed the best diagnostic performance (AUC 0.858, sensitivity 79.0%, specificity 84.2%).ConclusionAs a preliminary one, Lp-PLA2 demonstrates significant diagnostic value for DFO. The combination of Lp-PLA2 and ESR can significantly improve diagnostic accuracy of DFO.","PeriodicalId":94229,"journal":{"name":"The international journal of lower extremity wounds","volume":" ","pages":"15347346251340757"},"PeriodicalIF":0.0,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144056352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LncRNA SNHG14 Drives NLRP3 Inflammasome Activation in Diabetic Foot Ulcers: Mechanistic Insights and Diagnostic Implications. LncRNA SNHG14驱动NLRP3炎性体在糖尿病足溃疡中的激活：机制见解和诊断意义

The international journal of lower extremity wounds Pub Date : 2025-05-07 DOI: 10.1177/15347346251339713

Xincheng Liao, Zhengying Jiang, Zhonghua Fu, Mingzhuo Liu, Guanghua Guo

{"title":"LncRNA SNHG14 Drives NLRP3 Inflammasome Activation in Diabetic Foot Ulcers: Mechanistic Insights and Diagnostic Implications.","authors":"Xincheng Liao, Zhengying Jiang, Zhonghua Fu, Mingzhuo Liu, Guanghua Guo","doi":"10.1177/15347346251339713","DOIUrl":"10.1177/15347346251339713","url":null,"abstract":"BackgroundDiabetic foot (DF), a severe complication of diabetes mellitus (DM), poses significant challenges in early diagnosis and mechanistic understanding. This study investigates the expression patterns and clinical relevance of long non-coding RNA SNHG14 (lncRNA SNHG14) and the NLRP3 inflammasome in DF pathogenesis.MethodsA total of 176 DM patients (88 DF cases vs 88 Non-DF controls) admitted between September 2022 and February 2024 were enrolled. Serum SNHG14 and NLRP3 levels were quantified via qRT-PCR, while DF severity was categorized using the Wagner grading system. Pearson's correlation assessed SNHG14-NLRP3 interactions, Spearman's rank correlation evaluated their associations with Wagner grades, logistic regression identified independent risk factors, and ROC analysis determined diagnostic efficacy.ResultsDF patients exhibited significantly prolonged diabetes duration, elevated HbA1c%, FPG, and upregulated SNHG14/NLRP3 expression compared to Non-DF controls (P < 0.05). A robust positive correlation was observed between SNHG14 and NLRP3 (r = 0.7006, P < 0.0001). Multivariate logistic regression revealed diabetes duration (OR = 7.423, P < 0.0001), HbA1c (OR = 19.478, P = 0.002), SNHG14 overexpression (OR = 5941.653, P < 0.001), and NLRP3 upregulation (OR = 529.864, P = 0.036) as independent DF risk factors. Both SNHG14 (r = 0.5953) and NLRP3 (r = 0.5554) positively correlated with Wagner grades (P < 0.0001). ROC analysis demonstrated high diagnostic accuracy for SNHG14 (AUC = 0.8688) and NLRP3 (AUC = 0.8074), with combined detection further improving performance (AUC = 0.8773, sensitivity = 77.27%, specificity = 93.18%).ConclusionOverexpression of SNHG14 and NLRP3 is intricately linked to DF progression, metabolic dysregulation, and ulcer severity. Their combined use synergistically enhances diagnostic precision, highlighting transformative potential in DF management.","PeriodicalId":94229,"journal":{"name":"The international journal of lower extremity wounds","volume":" ","pages":"15347346251339713"},"PeriodicalIF":0.0,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144056775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0