The FEBS journal最新文献_第2页

Research highlights 研究重点

The FEBS journal Pub Date : 2024-11-13 DOI: 10.1111/febs.17315

Hajrah Khawaja

引用次数: 0

Viscosity regulates cell spreading and cell-extracellular matrix interactions. 粘度可调节细胞扩散以及细胞与细胞外基质的相互作用。

The FEBS journal Pub Date : 2024-11-11 DOI: 10.1111/febs.17306

Hugh Xiao, Xiangyu Gong, Seyma Nayir Jordan, Zixie Liang, Michael Mak

{"title":"Viscosity regulates cell spreading and cell-extracellular matrix interactions.","authors":"Hugh Xiao, Xiangyu Gong, Seyma Nayir Jordan, Zixie Liang, Michael Mak","doi":"10.1111/febs.17306","DOIUrl":"https://doi.org/10.1111/febs.17306","url":null,"abstract":"Fluid viscosity and osmolarity are among some of the underappreciated mechanical stimuli that cells can detect. Abnormal changes of multiple fluidic factors such as viscosity and osmolarity have been linked with diseases such as cystic fibrosis, cancer, and coronary heart disease. Changes in viscosity have been recently suggested as a regulator of cell locomotion. These novel studies focus on cell migration and spreading on glass substrates and through microchannels, and it remains a question whether viscosity impacts the cellular remodeling of extracellular matrices (ECMs). Here, we demonstrate that elevated viscosity induces cellular remodeling of collagen substrates and enhances cell spreading on ECM-mimetic substrates. Our results expand on recent work showing that viscosity induces increased cellular forces and demonstrates that viscosity can drive local ECM densification. Our data further show that microtubules, Ras-related C3 botulinum toxin substrate 1 (Rac1), actin-related protein 2/3 (Arp2/3) complex, Rho-associated protein kinase 1 (ROCK), and myosin are important regulators of viscosity-induced ECM remodeling. In the context of viscosity-induced cell spreading, cells cultured on glass and collagen substrates exhibit markedly different responses to pharmacological treatments, indicating that microtubules, Rac1, and Arp2/3 play distinct roles in regulating cellular spreading depending on the substrate. In addition, our results demonstrate that high osmotic pressures override viscosity-induced cell spreading by suppressing membrane ruffling. Our results demonstrate viscosity as a regulator of ECM remodeling and cell spreading in a fibrillar microenvironment. We also reveal a complex interplay between viscosity and osmolarity. We anticipate that our research can pave the way for future investigations into the crucial roles played by viscosity in both physiological and pathological conditions.","PeriodicalId":94226,"journal":{"name":"The FEBS journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The chemoreceptor controlling the Wsp-like transduction pathway in Halomonas titanicae KHS3 binds and responds to purine derivatives. 控制 Halomonas titanicae KHS3 中 Wsp 类传导途径的化学感受器与嘌呤衍生物结合并做出反应。

The FEBS journal Pub Date : 2024-11-11 DOI: 10.1111/febs.17320

Fernando E Ramos Ricciuti, Anabel Soldano, M Karina Herrera Seitz, Ana F Gasperotti, Alexandra Boyko, Kirsten Jung, Marco Bellinzoni, María-Natalia Lisa, Claudia A Studdert

{"title":"The chemoreceptor controlling the Wsp-like transduction pathway in Halomonas titanicae KHS3 binds and responds to purine derivatives.","authors":"Fernando E Ramos Ricciuti, Anabel Soldano, M Karina Herrera Seitz, Ana F Gasperotti, Alexandra Boyko, Kirsten Jung, Marco Bellinzoni, María-Natalia Lisa, Claudia A Studdert","doi":"10.1111/febs.17320","DOIUrl":"https://doi.org/10.1111/febs.17320","url":null,"abstract":"The chemosensory pathway HtChe2 from the marine bacterium Halomonas titanicae KHS3 controls the activity of a diguanylate cyclase. Constitutive activation of this pathway results in colony morphology alterations and an increased ability to form biofilm. Such characteristics resemble the behavior of the Wsp pathway of Pseudomonas. In this work, we investigate the specificity of Htc10, the only chemoreceptor coded within the HtChe2 gene cluster. The purine derivatives guanine and hypoxanthine were identified as ligands of the recombinantly produced Htc10 ligand-binding domain, with dissociation constants in the micromolar range, and its structure was solved by X-ray protein crystallography. The sensor domain of Htc10 adopts a double Cache folding, with ligands bound to the membrane-distal pocket. A high-resolution structure of the occupied guanine-binding pocket allowed the identification of residues involved in ligand recognition. Such residues were validated by site-directed mutagenesis and isothermal titration calorimetry analyses of the protein variants. Moreover, heterologous expression of Htc10 in a Pseudomonas putida mutant lacking the native Wsp chemoreceptor promoted biofilm formation, a phenotype that was further enhanced by Htc10-specific ligands. To our knowledge, this is the first description of binding specificity of a chemoreceptor that controls the activity of an associated diguanylate cyclase, opening the way for dynamic studies of the signaling behavior of this kind of sensory complex.","PeriodicalId":94226,"journal":{"name":"The FEBS journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Atg2 controls Drosophila hematopoiesis through the PVR/TOR signaling pathways. Atg2通过PVR/TOR信号通路控制果蝇造血。

The FEBS journal Pub Date : 2024-11-08 DOI: 10.1111/febs.17288

Bo Qin, Hongmei Xue, Xiaoran Wang, Hyonil Kim, Li Hua Jin

{"title":"Atg2 controls Drosophila hematopoiesis through the PVR/TOR signaling pathways.","authors":"Bo Qin, Hongmei Xue, Xiaoran Wang, Hyonil Kim, Li Hua Jin","doi":"10.1111/febs.17288","DOIUrl":"https://doi.org/10.1111/febs.17288","url":null,"abstract":"The hematopoietic system of Drosophila is a well-established genetic model for studying hematopoiesis mechanisms, which are strictly regulated by multiple signaling pathways. Autophagy-related 2 (Atg2) protein is involved in autophagosome formation through its lipid transfer function; however, other functions in animal development, especially the role of Atg2 in maintaining hematopoietic homeostasis, are unclear. Here, we show that Atg2 knockdown in the cortical zone (CZ) induced the proliferation and differentiation of mature plasmatocytes and disrupted progenitor maintenance in the medullary zone (MZ). We also observed the differentiation of lamellocytes among circulating hemocytes and in the lymph gland, which is rarely observed in healthy larvae. The above results on hematopoiesis disorders are due to Atg2 regulating the Drosophila PDGF/VEGF receptor (PVR) and target of rapamycin (TOR) in the CZ of lymph gland. In conclusion, we identified Atg2 as a previously undescribed regulator of hematopoiesis. Understanding the mechanism of maintenance of hematopoietic homeostasis in Drosophila will help us better evaluate human blood disorder-related diseases.","PeriodicalId":94226,"journal":{"name":"The FEBS journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142607863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structure of human phospholipase D3, a single-strand exonuclease associated with Alzheimer's disease. 与阿尔茨海默病有关的单链外切酶--人类磷脂酶 D3 的结构。

The FEBS journal Pub Date : 2024-11-08 DOI: 10.1111/febs.17319

Aleksandar Bijelic, Peter Macheroux

引用次数: 0

Obesity, white adipose tissue and cancer. 肥胖、白色脂肪组织与癌症

The FEBS journal Pub Date : 2024-11-04 DOI: 10.1111/febs.17312

Estel Solsona-Vilarrasa, Karen H Vousden

{"title":"Obesity, white adipose tissue and cancer.","authors":"Estel Solsona-Vilarrasa, Karen H Vousden","doi":"10.1111/febs.17312","DOIUrl":"https://doi.org/10.1111/febs.17312","url":null,"abstract":"White adipose tissue (WAT) is crucial for whole-body energy homeostasis and plays an important role in metabolic and hormonal regulation. While healthy WAT undergoes controlled expansion and contraction to meet the body's requirements, dysfunctional WAT in conditions like obesity is characterized by excessive tissue expansion, alterations in lipid homeostasis, inflammation, hypoxia, and fibrosis. Obesity is strongly associated with an increased risk of numerous cancers, with obesity-induced WAT dysfunction influencing cancer development through various mechanisms involving both systemic and local interactions between adipose tissue and tumors. Unhealthy obese WAT affects circulating levels of free fatty acids and factors like leptin, adiponectin, and insulin, altering systemic lipid metabolism and inducing inflammation that supports tumor growth. Similar mechanisms are observed locally in an adipose-rich tumor microenvironment (TME), where WAT cells can also trigger extracellular matrix remodeling, thereby enhancing the TME's ability to promote tumor growth. Moreover, tumors reciprocally interact with WAT, creating a bidirectional communication that further enhances tumorigenesis. This review focuses on the complex interplay between obesity, WAT dysfunction, and primary tumor growth, highlighting potential targets for therapeutic intervention.","PeriodicalId":94226,"journal":{"name":"The FEBS journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142577340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Psr1 phosphatase regulates pre-mRNA splicing through spliceosomal B complex factor Snu66. Psr1 磷酸酶通过剪接体 B 复合因子 Snu66 调节前 mRNA 的剪接。

The FEBS journal Pub Date : 2024-11-01 DOI: 10.1111/febs.17314

Amjadudheen Varikkapulakkal, Balashankar R Pillai, Shravan Kumar Mishra

引用次数: 0

A one-day journey to the suburbs: circadian clock in the Drosophila visual system. 郊区一日游：果蝇视觉系统中的昼夜节律。

The FEBS journal Pub Date : 2024-11-01 DOI: 10.1111/febs.17317

Milena Damulewicz, Gabriella M Mazzotta

引用次数: 0

A structural snapshot of the multiple working states of the Mpox virus helicase-primase D5. Mpox 病毒螺旋酶-primase D5 多种工作状态的结构快照。

The FEBS journal Pub Date : 2024-10-29 DOI: 10.1111/febs.17292

Yingying Guo, Renhong Yan

引用次数: 0

Interleukin-11 receptor is an alternative α-receptor for interleukin-6 and the chimeric cytokine IC7. 白细胞介素-11 受体是白细胞介素-6 和嵌合细胞因子 IC7 的另一种 α 受体。

The FEBS journal Pub Date : 2024-10-29 DOI: 10.1111/febs.17309

Hendrik T Weitz, Julia Ettich, Puyan Rafii, Christoph Wittich, Laura Schultz, Nils C Frank, Denise Heise, Matthias Krusche, Juliane Lokau, Christoph Garbers, Kristina Behnke, Doreen M Floss, Harald Kolmar, Jens M Moll, Jürgen Scheller

{"title":"Interleukin-11 receptor is an alternative α-receptor for interleukin-6 and the chimeric cytokine IC7.","authors":"Hendrik T Weitz, Julia Ettich, Puyan Rafii, Christoph Wittich, Laura Schultz, Nils C Frank, Denise Heise, Matthias Krusche, Juliane Lokau, Christoph Garbers, Kristina Behnke, Doreen M Floss, Harald Kolmar, Jens M Moll, Jürgen Scheller","doi":"10.1111/febs.17309","DOIUrl":"https://doi.org/10.1111/febs.17309","url":null,"abstract":"The cytokine interleukin 6 (IL-6) signals via the IL-6 α-receptor (IL-6Rα or IL-6R) in complex with the gp130 β-receptor. Cell type restricted expression of the IL-6R limits the action of IL-6 mainly to hepatocytes and some immune cells. Here, we show that IL-6 also binds to the IL-11 α receptor (IL-11Rα or IL-11R) and induces signaling via IL-11R:gp130 complexes, albeit with a lower affinity compared to IL-11. Antagonistic antibodies directed against IL-11R, but not IL-6R, inhibit IL-6 signaling via IL-11R:gp130 receptor complexes. Notably, IL-11 did not cross-react with IL-6R. IL-11R has also been identified as an alternative α receptor for the CNTF/IL-6-derived chimeric cytokine IC7, which has recently been shown to induce weight loss in mice. Accordingly, the effects of therapeutic monoclonal antibodies against IL-6 or IL-6R, which both block IL-6 signaling, may be slightly different. These findings provide new insights into IL-6 signaling and therefore offer new potential therapeutic intervention options in the future.","PeriodicalId":94226,"journal":{"name":"The FEBS journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142549911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0