Polish journal of veterinary sciences最新文献_第5页

Integrated analysis of differential gene expression profiles in porcine alveolar macrophages induced by Mycoplasma hyopneumoniae strain 232. 猪肺炎支原体232株诱导肺泡巨噬细胞差异基因表达谱的综合分析。

Polish journal of veterinary sciences Pub Date : 2024-09-01 DOI: 10.24425/pjvs.2024.151735

G Wang, J Li, Y Liu

{"title":"Integrated analysis of differential gene expression profiles in porcine alveolar macrophages induced by Mycoplasma hyopneumoniae strain 232.","authors":"G Wang, J Li, Y Liu","doi":"10.24425/pjvs.2024.151735","DOIUrl":"https://doi.org/10.24425/pjvs.2024.151735","url":null,"abstract":"Porcine alveolar macrophages (PAMs) can resist infection caused by Mycoplasma hyopneumoniae (Mhp) through phagocytosis. However, it is unknown what gene expression changes occur in PAM after Mhp stimulation. Therefore, the differential gene expression (DGE) profiling technique was employed to analyze differentially expressed genes in PAMs infected with Mhp strain 232. Eighty-six and 889 differentially expressed (DE) genes were identified in PAMs at 12 hours post-infection (hpi) and 24 hpi. Using Gene Ontology (GO) analysis, DE genes were involved in 54 (12 hpi) and 128 (24 hpi) GO enrichment items. Using Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, DE genes were involved in 101 (12 hpi) and 250 (24 hpi) KEGG enrichment items. Using Ingenuity Pathway Analysis (IPA), DE genes were connected, forming 25 internally interacting subnetworks. STRING analysis revealed 131 proteins encoded by DE genes involved in network interactions. Five novel genes were closely related to clinical symptoms and pathological changes of Mycoplasma hyopneumoniae. This is the first study to investigate PAM transcriptional responses to Mhp infection using the DGE profiling technique.","PeriodicalId":94175,"journal":{"name":"Polish journal of veterinary sciences","volume":"27 3","pages":"419-430"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142908121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ameliorative effects of Ganoderma lucidum (Reishi) on testicular tissue of rats exposed to bisphenol A. 灵芝对双酚A暴露大鼠睾丸组织的改善作用。

Polish journal of veterinary sciences Pub Date : 2024-09-01 DOI: 10.24425/pjvs.2024.151741

T C Acısu, S A Akarsu, M Sönmez, A Yüce, S Çeribaşı, S Dayan Cinkara, Ş Özer Kaya, G Türk, S Gür

{"title":"Ameliorative effects of Ganoderma lucidum (Reishi) on testicular tissue of rats exposed to bisphenol A.","authors":"T C Acısu, S A Akarsu, M Sönmez, A Yüce, S Çeribaşı, S Dayan Cinkara, Ş Özer Kaya, G Türk, S Gür","doi":"10.24425/pjvs.2024.151741","DOIUrl":"https://doi.org/10.24425/pjvs.2024.151741","url":null,"abstract":"Bisphenol A (BPA), an endocrine disrupting chemical, is an environmental toxicant widely used in the production of polycarbonate plastics, epoxy resins and paints. Ganoderma lucidum (GDL) is a plant with biological activities widely used in Chinese medicine. The present study aims to determine the effects of GDL against testicular dysfunction in rats exposed to BPA. For this purpose, a total of 24 Sprague Dawley rats, 6 rats in each group, were used in the study. Rats were administered 25 mg/kg/bw BPA and 300 mg/kg/bw GDL by oral gavage for 8 weeks. After the treatments, the rats were sacrificed, and testicular tissues were removed. One of the testes was used for biochemical analyses and the other for histopathologic examinations. The caudal part of the epididymis was trimmed, and semen was obtained. As a result, BPA increased MDA level in blood and testicular tissue, while it decreased CAT, GPx activity and GSH level. GDL treatment provided protection from the impaired oxidant balance (p<0.001). Furthermore, BPA caused decreased epididymal sperm motility and density, vesicular seminalis weight and blood testosterone levels, increased testicular and epididymal tissue weight (p<0.001). Histopathological examination revealed that BPA caused narrowing in testicular tubules and apoptosis, decreased germinal cell thickness and androgen receptor number. It was determined that GDL administration preserved testicular histology. As a result, it was determined that BPA caused toxicity in the testicular tissue of rats, whereas GDL administration was ameliorative.","PeriodicalId":94175,"journal":{"name":"Polish journal of veterinary sciences","volume":"27 3","pages":"459-467"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142908088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptome analysis reveals heat shock protein 70 (HSP70) induced by tembusu virus infection is associated with immune responses. 转录组分析显示，坦布苏病毒感染诱导的热休克蛋白70 （HSP70）与免疫应答有关。

Polish journal of veterinary sciences Pub Date : 2024-09-01 DOI: 10.24425/pjvs.2024.151736

D Zhao, K Han, L Zhang, X Huang, Q Liu, J Yang, Y Liu, Y Li, F Wu

{"title":"Transcriptome analysis reveals heat shock protein 70 (HSP70) induced by tembusu virus infection is associated with immune responses.","authors":"D Zhao, K Han, L Zhang, X Huang, Q Liu, J Yang, Y Liu, Y Li, F Wu","doi":"10.24425/pjvs.2024.151736","DOIUrl":"https://doi.org/10.24425/pjvs.2024.151736","url":null,"abstract":"The outbreak and prevalence of tembusu virus (TMUV) endanger the breeding industry of waterfowls. However, little is known about the molecular mechanism underlying TMUV infection. It was reported that heat shock protein 70 (HSP70) was a positive regulator of the infection of TMUV. In order to study the interactions between HSP70 and host immune response to TMUV infection, TMUV-infected cells with or without HSP70 inhibitor were harvested and subjected to deep sequencing to identify genes differentially expressed. We found 43 differentially expressed genes (DEGs) in HSP70 inhibitor-treated and mock-treated TMUV-infected DF-1 cells. Of these DEGs, 39 genes were down-regulated significantly. Gene Ontology analysis suggested that the DEGs were mainly involved in biological process, cellular component and molecular function. Kyoto Encyclopedia of Genes and Genomes analysis showed that the DEGs mainly related to the activation of innate immune response, including RIG-I-like receptor, toll-like receptor and NF-κB signaling pathway. Also, 12 down-regulated immune-related DEGs were selected for confirmation by reverse transcription quantitative real-time PCR verification, all these genes showed consistent expression between the result of reverse transcription quantitative real-time PCR and transcriptomic sequencing. These results revealed the important role of HSP70 in facilitating the innate immune response induced by TMUV infection. This is first to access the role of HSP70 in host response to TMUV infection, which provides a basis for further study of the pathogenesis of TMUV and contributes to the elucidation of TMUV-host interactions.","PeriodicalId":94175,"journal":{"name":"Polish journal of veterinary sciences","volume":"27 3","pages":"431-440"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142908176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunolocalization of sodium-dependent glucose co-transporter 1 and sodium-dependent glucose co-transporter 2 in chicken's (Gallus gallus domesticus) kidneys. 鸡肾脏中钠依赖性葡萄糖共转运体 1 和钠依赖性葡萄糖共转运体 2 的免疫定位。

Polish journal of veterinary sciences Pub Date : 2024-09-01 DOI: 10.24425/pjvs.2024.149363

C Allmang, P Hussar, I Dūrītis, T Järveots

{"title":"Immunolocalization of sodium-dependent glucose co-transporter 1 and sodium-dependent glucose co-transporter 2 in chicken's (Gallus gallus domesticus) kidneys.","authors":"C Allmang, P Hussar, I Dūrītis, T Järveots","doi":"10.24425/pjvs.2024.149363","DOIUrl":"https://doi.org/10.24425/pjvs.2024.149363","url":null,"abstract":"In homeostasis, which plays an important role in the proper functioning and maintenance of the internal functioning of the body, kidneys play a key role in being responsible for the proper homeostasis of glucose. Among glucose transporters, sodium-dependent glucose co-transporters (SGLTs) have a major role in the kidney's ability to reabsorb glucose. Although the localization of these transporters has been extensively studied in mammals, there are still gaps in knowledge of the localization of SGLTs in birds of different age groups. The aim of this study was to immunolocalize in kidneys of hen chickens of different ages the sodium-dependent glucose co-transporters SGLT1 and SGLT2, comparing the localization between different age groups. The kidneys derived from 32 hen chickens (Gallus gallus domesticus) were devided equally into four age groups: 3, 7, 14, and 20 day old broilers, 8 indivuals in each group. The polyclonal primary antibodies Rabbit anti- SGLT1 and Rabbit anti-SGLT2 (Abcam, UK) were used together with the corresponding IHC kit (Abcam, UK). The results were visualized photographically using an AxioCam HRc camera (Germany) connected to a Zeiss Axioplan-2 Imaging microscope (Germany). The study revealed similar immunolocalization of SGLT1 and SGLT2 in the apical part of cells of proximal renal tubules in hen chickens' kidneys in all age groups. Strong staining of SGLT2 was noted also in the cytoplasm of epithelial cells of the proximal straight and convoluted tubules. Based on our study, the kidney tissue of newly hatched chickens is ready immediately after hatching for glucose reabsorption and transport, similarily to that of three-week-old chicks.","PeriodicalId":94175,"journal":{"name":"Polish journal of veterinary sciences","volume":"27 3","pages":"323-328"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142908116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Montelukast potentiates the relaxing effect of nifedipine in the porcine myometrium. 孟鲁司特能增强硝苯地平对猪子宫肌层的松弛作用。

Polish journal of veterinary sciences Pub Date : 2024-09-01 DOI: 10.24425/pjvs.2024.151731

W Markiewicz, A Łukawska, N Smolińska, O Yilmaz, A Burmańczuk, J J Jaroszewski

{"title":"Montelukast potentiates the relaxing effect of nifedipine in the porcine myometrium.","authors":"W Markiewicz, A Łukawska, N Smolińska, O Yilmaz, A Burmańczuk, J J Jaroszewski","doi":"10.24425/pjvs.2024.151731","DOIUrl":"https://doi.org/10.24425/pjvs.2024.151731","url":null,"abstract":"This study analysed the influence of montelukast (MON), a cysteinyl leukotriene receptor antagonist, and nifedipine, an L-type voltage-gated Ca2+ channel blocker, on the contractility of the porcine uterine smooth muscle. Myometrial strips were collected from the sexually immature (n=8), cyclic (12-14 days of the oestrous cycle; n=8) and pregnant (27-28 days of pregnancy; n=8) gilts and stimulated with a) MON or nifedipine at concentrations of 10-8-10-4 M and b) increasing concentrations of nifedipine after previous administration of MON at a concentration of 10-4 M. The changes in the tension, amplitude and frequency of contractions were determined with the Hugo Sachs Elektronik equipment for measuring isometric contractions. Stimulation of the uterine strips with high concentrations of MON significantly reduced the amplitude of contractions in both the cyclic and pregnant group and the frequency of contractions in the pregnant group. In high concentrations, nifedipine significantly decreased the amplitude and frequency of contractions in all examined groups. Nifedipine administered after MON pre-treatment significantly decreased the tension in the pregnant group and the amplitude and frequency of contractions in all examined groups; this effect was more evident in comparison with nifedipine used alone. The results obtained indicate that the influence of MON and nifedipine on the contractile activity of the porcine uterus is dependent on the physiological status of the animal. Moreover, the blockage of the CysLT1 receptor enhances the relaxing effect of nifedipine.","PeriodicalId":94175,"journal":{"name":"Polish journal of veterinary sciences","volume":"27 3","pages":"379-388"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142908172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential molecular targets and pathways of a traditional Chinese medicine formula for bovine endometritis identified by network pharmacology. 网络药理学鉴定牛子宫内膜炎中药方剂的潜在分子靶点和途径。

Polish journal of veterinary sciences Pub Date : 2024-09-01 DOI: 10.24425/pjvs.2024.151730

Q Li, Zh Cao, X Ling, P Sun, W Yin, K Fan, N Sun, H Li

{"title":"Potential molecular targets and pathways of a traditional Chinese medicine formula for bovine endometritis identified by network pharmacology.","authors":"Q Li, Zh Cao, X Ling, P Sun, W Yin, K Fan, N Sun, H Li","doi":"10.24425/pjvs.2024.151730","DOIUrl":"https://doi.org/10.24425/pjvs.2024.151730","url":null,"abstract":"Bovine endometritis has become a persistent issue in the global dairy business, resulting in huge economic losses. Due to their numerous positive benefits, Chinese herbal medicines (CHMs) have recently demonstrated remarkable pharmacological potential against endometritis. The objective of this study was to investigate the effects and elucidate the underlying mechanisms of the Yimucao formula (YMF) that involves five herbs in lactation cows under endometritis conditions. Initially, the possible impacts of YMF on cows with endometritis were assessed. Then, using network pharmacology, potential molecular processes by which the YMF prevents endometritis were suggested. The findings demonstrated a considerable improvement in endometritis-related clinical complaints following YMF treatment. Mechanically, 150 active compounds were identified from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP); of these, quercetin, kaempferol, beta-sitosterol, apigenin, isorhamnetin, and sitogluside were the most prevalent active substances. The NCBI gene, GeneCard, and OMIM databases had 110 genes linked to endometritis. The intersection of these targets with the 213 active ingredient targets produced 17 common targets, of which BCL2, IL-6, MMP9, HIF1α, TNF, IL-1β, and ICAM1 were the top 7 core targets. According to the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment data, atherosclerosis, fluid shear stress, and the AGE-RAGE signaling pathway are the primary causes of YMF's anti-endometritis action. Finally, our results indicate that the YMF works on endometritis through various and multi-targeted signaling pathways, which provide reference for clinical practice, based on network pharmacology and molecular docking.","PeriodicalId":94175,"journal":{"name":"Polish journal of veterinary sciences","volume":"27 3","pages":"363-377"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142908175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Understanding ultrasonographic renal length-to-aorta ratio in Sighthounds: A breed specific study in Chippiparai dogs. 了解视觉犬的超声肾长主动脉比：一项对Chippiparai犬的品种特异性研究。

Polish journal of veterinary sciences Pub Date : 2024-09-01 DOI: 10.24425/pjvs.2024.151742

T P Lenka, G Senthil Kumar, D Sumathi, S Saravanan, A Serma Saravana Pandian, R Ravi

引用次数: 0

Assessment of left atrial size, left atrial volume and left ventricular function, and its relation to spontaneous echocardiographic contrast in cats with hypertrophic cardiomyopathy: A preliminary study. 肥厚型心肌病猫左心房大小、左心房容积和左心室功能的评估及其与自发超声心动图对比的关系：初步研究。

Polish journal of veterinary sciences Pub Date : 2024-09-01 DOI: 10.24425/pjvs.2024.151743

E Colakoglu, K Sevim, U Kaya

引用次数: 0

Molecular detection and subtype distribution of Blastocystis sp. from shelter dogs and cats in Van, Turkey: First report of ST10 in cats and ST1, ST10 and ST30 in dogs. 土耳其Van地区收容犬和猫囊虫的分子检测和亚型分布：首次报道猫中ST10和狗中ST1、ST10和ST30。

Polish journal of veterinary sciences Pub Date : 2024-09-01 DOI: 10.24425/pjvs.2024.151732

A Ayan, B Aslan Çelik, Ö Y Çelik, A B Yılmaz, Ö Orunç Kılınç, Ö Oktay Ayan

引用次数: 0

Use of immunocontraception in the regulation of male goat sexual activity. 免疫避孕在公山羊性活动调节中的应用。

Polish journal of veterinary sciences Pub Date : 2024-09-01 DOI: 10.24425/pjvs.2024.151737

A Hochmanova, R Novotny, I Simkova, M Plesko, V Bína, E Indrova

{"title":"Use of immunocontraception in the regulation of male goat sexual activity.","authors":"A Hochmanova, R Novotny, I Simkova, M Plesko, V Bína, E Indrova","doi":"10.24425/pjvs.2024.151737","DOIUrl":"https://doi.org/10.24425/pjvs.2024.151737","url":null,"abstract":"This study focused on continuous monitoring of the immunocontraceptive effect of Improvac® vaccine on the sexual activity of male goats determined by measuring plasma testosterone levels, testicular biometric and ejaculate examination. The animals in the experimental group (n=12) were administered two doses of 2 ml of Improvac® at a four-week interval; the animals in the control group (n=5) received 2 ml of saline. Blood collection, semen collection and testicular measurements were performed at 14-day intervals. A total of 8 samples were collected from each animal. In 9 animals a significant decrease (p<0.05) in testosterone concentration was observed two weeks after the first dose. At the end of the experiment (16 weeks), eight goats reached a testosterone concentration below the detection limit and one goat had a concentration of 0.47nmol/L. The testicular size was significantly (p<0.01) smaller four weeks after the first dose. At the end of the experiment, the testicular size was approximately three times smaller (p<0.001). Motility was 0% in two goats at the end of the experiment, 1% in one animal and 10% in one animal. The median sperm concentration was significantly lower (p<0.01) at the end of the study. A significant (p<0.0001) shift in the percentage of morphological changes was recorded eight weeks after the first administration. At the end of the study, there were five animals with azoospermia, two with 100% morphologically altered sperm, one with 99% and one with 96% morphologically altered sperm. In the three male goats, a significant skin reaction occurred after the first application, which resulted in an inadequate response to the treatment. Our results show that Improvac had a significant effect on the sexual function and sperm production in 9 out of 12 male goats.","PeriodicalId":94175,"journal":{"name":"Polish journal of veterinary sciences","volume":"27 3","pages":"441-450"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142908178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0