Photodiagnosis and photodynamic therapy最新文献

{"title":"Generational shift for clinical application of the QLF system for evaluating tooth wear.","authors":"Sang-Kyeom Kim, Eun-Song Lee, Baek-Il Kim","doi":"10.1016/j.pdpdt.2024.104413","DOIUrl":"10.1016/j.pdpdt.2024.104413","url":null,"abstract":"<p><strong>Background: </strong>No study has quantitatively assessed tooth wear using a clinical quantitative light-induced fluorescence (QLF) system. This study aimed to compare fluorescence parameters (ΔF_wear) between the research QLF system (QLF-D) and clinical QLF system (Qraycam Pro) and evaluate the validity of both systems in detecting dentin exposure from tooth wear.</p><p><strong>Methods: </strong>Thirty-five human molars and premolars were collected. Two blinded examiners conducted evaluations. Images from QLF-D and Qraycam Pro were captured and analyzed by the first examiner to calculate ΔF_wear, representing the maximum fluorescence intensity for occlusal wear. The stage of tooth wear was determined by the second examiner using the tooth wear index (TWI). The area of interest (AOI) was determined as the cusp without defects, such as caries or fractures. Only areas mutually agreed by both examiners were included in analysis. The Kruskal-Wallis test was conducted to assess differences in ΔF_wear between two devices. ROC analysis evaluated the validity of both systems in determining dentin exposure using AUROC.</p><p><strong>Results: </strong>Thirty-eight AOIs were analyzed. ΔF_wear significantly increased with higher TWI scores. Median ΔF_wear for TWI scores (0, 1, and 2) were 6.9 %, 10.3 %, and 24.8 %, respectively, for QLF-D, and 5.7 %, 7.7 %, and 23.9 %, respectively, for Qraycam Pro. No significant differences in ΔF_wear were observed between QLF-D and Qraycam Pro for any TWI score. The AUROC for both systems was 0.95.</p><p><strong>Conclusion: </strong>ΔF_wear was comparable between QLF-D and Qraycam Pro and demonstrated high validity in detecting dentin exposure. These findings support the clinical application of the QLF for quantitative tooth wear assessment.</p>","PeriodicalId":94170,"journal":{"name":"Photodiagnosis and photodynamic therapy","volume":" ","pages":"104413"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142694050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of repeated low-level red-light exposure on choroidal thickness and blood flow in pediatric patients: A SS-OCTA study.","authors":"Huihang Wang, Huifen Zhong, Jingjin Zhang, Wei Wei, Xiaoyuan Cui, Weidong Zheng","doi":"10.1016/j.pdpdt.2024.104412","DOIUrl":"10.1016/j.pdpdt.2024.104412","url":null,"abstract":"<p><strong>Purpose: </strong>To evaluate the impact of repeated low-level red-light (RLRL) therapy on choroidal thickness and blood flow in pediatric myopia.</p><p><strong>Methods: </strong>A three-month trial (April 1, 2023 - September 30, 2023) was conducted involving 44 children (ages 6-16) with myopia. Participants underwent RLRL therapy at home twice daily for five days per week, with each session lasting three minutes. Assessments at baseline, one month, and three months included cycloplegic refraction, best-corrected visual acuity (BCVA), intraocular pressure (IOP), ocular biometrics, swept-source optical coherence tomography angiography (SS-OCTA), slit-lamp, and fundus examinations.</p><p><strong>Results: </strong>The study included 44 children (average age: 9.79 years; 56.82 % male). RLRL therapy significantly increased subfoveal choroidal thickness (Baseline: 272.82 ± 64.01 μm; 1-month: 297.77 ± 72.94 μm; 3-month: 298.77 ± 77.17 μm, p = 0.001), reduced axial length (Baseline: 24.97 ± 1.47 mm; 3-month: 24.88 ± 1.38 mm, p = 0.002), and showed a marginal regression in spherical equivalent (p = 0.055). Significant elevations in choroidal vessel volume and thickness were noted, with positive correlations intensifying with distance from the fovea.</p><p><strong>Conclusion: </strong>RLRL therapy shows promise in managing pediatric myopia by increasing choroidal vessel volume and thickness, potentially mitigating myopia progression.</p>","PeriodicalId":94170,"journal":{"name":"Photodiagnosis and photodynamic therapy","volume":" ","pages":"104412"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142695801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diminished retinal microcirculation in mild preeclampsia without obvious retinopathy in high-altitude native Tibetans by OCTA.","authors":"Chong Tang, Shuren Pan, Xiangfang Zeng, Xinlu Fu, Jiaxuan Deng, Kai Shi","doi":"10.1016/j.pdpdt.2024.104396","DOIUrl":"10.1016/j.pdpdt.2024.104396","url":null,"abstract":"<p><strong>Background: </strong>To investigate the retinal microcirculation in mild preeclampsia (PE) without obvious retinopathy in high-altitude native Tibetans by optical coherence tomography angiography (OCTA).</p><p><strong>Methods: </strong>This study enrolled 64 mild preeclamptic pregnant women without retinopathy (group PPW), 63 healthy pregnant women (group HPW), and 61 healthy non-pregnant women (group HNPW). All participants were high-altitude native Tibetans who underwent OCTA, retinal thickness (RT), vascular density (VD), perfusion density (PD) and foveal avascular zone (FAZ) parameters were measured in the macula. Differences in these parameters among the groups were compared, and the correlations of these parameters with blood pressure were evaluated.</p><p><strong>Results: </strong>In the entire macula, VD and PD were significantly lower in group PPW than in group HPW and HNPW (p < 0.05). In the subregions, VD was significantly different in fovea, parafovea and perifovea, PD was significantly different in parafovea and perifovea among the three groups (p < 0.05), the further pairwise comparisons showed VD and PD in parafovea and perifovea were lower in group PPW than in group HPW, and VD and PD in perifovea were lower in group PPW than in group HNPW (p < 0.05). RT and FAZ parameters did not differ among the groups (P > 0.05). VD and PD exhibited negative correlations with SBP and DBP (p < 0.05).</p><p><strong>Conclusion: </strong>OCTA can objectively evaluate diminished microcirculation in mild PE without obvious retinopathy among high-altitude native Tibetans, and provide a crucial time window for early screening of PE-induced retinopathy.</p>","PeriodicalId":94170,"journal":{"name":"Photodiagnosis and photodynamic therapy","volume":" ","pages":"104396"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of efficacy of Hemoporfin-PDT in the treatment of PWS birthmarks of young children previously received PDL treatment.","authors":"Yawen Wang, Jun Zhou, Chunzhi Qi, Youbao Li, Die Hu, Rongrui Zhang, Shengzhi Mu, Zhao Wang, Songmei Geng, Weihui Zeng, Jing Liu","doi":"10.1016/j.pdpdt.2024.104418","DOIUrl":"10.1016/j.pdpdt.2024.104418","url":null,"abstract":"<p><strong>Background: </strong>Hemoporfin-mediated photodynamic therapy (Hemoporfin-PDT) is considered a safe and effective treatment for port-wine stains (PWS). This study aims to investigate the influence of prior pulse dye laser (PDL) treatments history on the effectiveness of Hemoporfin-PDT in young children aged 1-3 years with PWS.</p><p><strong>Methods: </strong>Data was gathered for individuals with PWS aged 1-3 years who received two or more Hemoporfin-PDT treatments. The study population was stratified into two groups: Cohort 1 (No PDL) comprised patients without a history of PDL treatment, while Cohort 2 (Prior PDL) consisted of patients with a history of PDL treatment. An analysis was conducted to investigate the relationship between treatment efficacy and variables including gender, age, location, and type of PWS.</p><p><strong>Results: </strong>There were a total of 220 cases in the No PDL group and 53 cases in the Prior PDL group. It was observed that prior PDL treatment history did not impact the efficacy of Hemoporfin-PDT in treating PWS among young children. Both the type and location of PWS significantly influence the clinical effectiveness of Hemoporfin-PDT for PWS (P = 0.040, P < 0.001), while gender and age show no association with treatment outcomes (P > 0.05). Notably, the peripheral face might be an independent influencing factor for high efficacy of Hemoporfin-PDT of the facial regions (P < 0.05).</p><p><strong>Conclusion: </strong>Previous PDL treatment history does not impact the effectiveness of Hemoporfin-PDT therapy in young children with PWS.</p>","PeriodicalId":94170,"journal":{"name":"Photodiagnosis and photodynamic therapy","volume":" ","pages":"104418"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142735425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pyropheophorbide-α methyl ester-mediated photodynamic therapy triggers pyroptosis in osteosarcoma cells via the ROS/caspase-3/GSDME pathway.","authors":"Qiu Huang, Yong Tao, Ye Zhang, Yuxing Chen, Fuqiang Tan, Yunsheng Ou","doi":"10.1016/j.pdpdt.2024.104427","DOIUrl":"10.1016/j.pdpdt.2024.104427","url":null,"abstract":"<p><strong>Background: </strong>Pyropheophorbide-α methyl ester-mediated photodynamic therapy(MPPa-PDT) is a candidate treatment for solid tumors, including osteosarcoma. Pyroptosis has garnered significant attention in cancer research due to its pro-inflammatory and immunomodulatory nature. This study investigated the mechanism and role of MPPa-PDT-induced pyroptosis in osteosarcoma cells.</p><p><strong>Methods: </strong>We treated human osteosarcoma 143b and HOS cells with MPPa at concentrations of 0.5 μM and 0.25 μM, respectively, then irradiated the cells with LED light at 630 nm wavelength with an energy density of 4.8 J/cm². Cell viability and apoptosis ratio were detected using CCK-8 and Annexin V-Propidium Iodide staining, respectively. Intracellular reactive oxygen species (ROS) levels and mitochondrial membrane potential (MtΔψ) were assessed using 2',7'-Dichlorofluorescin diacetate, and JC-1 staining kits, respectively. Scanning Electron Microscopy (SEM) was utilized to examine cell ultrastructure. The morphological changes of the cells were observed by an inverted microscope. Western blotting analysis was conducted to measure protein levels. To elucidate the mechanism and role, we re-evaluated relevant parameters after pretreating with NAC,Si caspase-3, and Si GSDME.</p><p><strong>Results: </strong>MPPa-PDT inhibited the activity of osteosarcoma 143b and HOS cells and induced pyroptosis with mitochondrial damage, ROS aggregation, and activation of Caspase-3 and GSDME. The effects of MPPa-PDT on the activity and apoptosis of osteosarcoma cells were partially reversed after pretreating with Si GSDME. After NAC pretreatment, the activation of pyroptosis and Caspase-3 induced by MPPa-PDT was partially reversed. After Si Caspase-3 pretreatment, the pyroptosis induced by MPPa-PDT was partially reversed.</p><p><strong>Conclusion: </strong>MPPa-PDT can induce pyroptosis in osteosarcoma cells, which has the effect of enhancing apoptotic processes. Mitochondrial damage and ROS/caspase-3/GSDME pathway are the possible mechanisms of pyroptosis induced by MPPa-PDT.</p>","PeriodicalId":94170,"journal":{"name":"Photodiagnosis and photodynamic therapy","volume":" ","pages":"104427"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142775962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Choroidal vascular parameters in patients with type 2 diabetes mellitus.","authors":"Isabella Pellegrino, Mariagrazia Avella","doi":"10.1016/j.pdpdt.2024.104423","DOIUrl":"10.1016/j.pdpdt.2024.104423","url":null,"abstract":"","PeriodicalId":94170,"journal":{"name":"Photodiagnosis and photodynamic therapy","volume":" ","pages":"104423"},"PeriodicalIF":0.0,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142775948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of fluorescence intensity of protoporphyrin IX as observed on the screen of different cystoscopic systems.","authors":"Hideo Fukuhara, Takahiro Nishimura, Yu Shimojo, Keiji Inoue","doi":"10.1016/j.pdpdt.2024.104425","DOIUrl":"10.1016/j.pdpdt.2024.104425","url":null,"abstract":"<p><strong>Background: </strong>Photodynamic diagnosis-assisted transurethral resection of bladder tumor (PDD-TURBT) is an important and useful intervention used for removing non-invasive bladder cancer (NMIBC). Several PDD devices can be used in clinical practice, but few reports have compared them.In this study, we examined the differences in detected fluorescence intensity for each PDD device.</p><p><strong>Methods: </strong>The PDD device was used from OLYMPUS, Richard Wolf, and Karl Storz. Light power and illuminance from endoscope were measured. For each PDD device, fluorescence intensity was measured at 3 cm and 8 cm distance from the protoporphyrin IX (PpIX) phantom to the cystoscope.</p><p><strong>Results: </strong>The variation of excitation light power with observation distance were 35.0 %, 27.0 %, and 28.2 % for OLYMPUS, Richard Wolf, and Karl Storz, respectively. The variation of fluorescence intensity with observation distance were 132.8 %, 120.5 %, and 49.8 % for OLYMPUS, Richard Wolf, and Karl Storz, respectively.</p><p><strong>Conclusions: </strong>PDD devices used in clinical practice show both some performance differences between the PDD systems themselves, and also differences in observed fluorescence intensity.</p>","PeriodicalId":94170,"journal":{"name":"Photodiagnosis and photodynamic therapy","volume":" ","pages":"104425"},"PeriodicalIF":0.0,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142756074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Caries affected Dentin biomodification using Bromelain, Riboflavin photosensitizer via UVA, and Chitosan Nanoparticles on shear bond strength and microleakage scores: A SEM evaluation.","authors":"Khold Alahdal, Ahmed Maawadh, Laila AlDeeb, Thamer Almohareb, Ahoud S Alshamrani, Ali Alrahlah","doi":"10.1016/j.pdpdt.2024.104419","DOIUrl":"10.1016/j.pdpdt.2024.104419","url":null,"abstract":"<p><strong>Aims: </strong>Assessment of the impact of different dentin bio-modifiers i.e., Bromelain, Riboflavin photosensitizer (RFP/Ultraviolet-A), and Chitosan nanoparticles (CHNPs) on the shear bond strength (SBS) and microleakage of composite bonded to acid etched carious affected dentin (CAD).</p><p><strong>Materials and methods: </strong>Sixty-four human molars in which carious lesions extend till the middle third of the dentin were included. The infected dentin was removed and CAD was exposed. All specimens were categorized into four groups based on the application of dentin bio-modifier (n = 16) Group 1 (No dentin modifier), Group 2 (Bromelain), Group 3 (RFP/UVA), and Group 4 (CHNPs). Dental adhesive application and composite buildup were performed. A dye penetration test was used to assess marginal leakage. Resin CAD interface assessment was performed using SEM. For SBS and failure pattern analysis, a universal testing machine and stereomicroscope were used respectively. One-way ANOVA followed by Bonferroni's posthoc test was employed to analyze the differences among various groups (p≤0.05) RESULTS: The lowest microleakage (13.27 ± 0.10) and highest SBS (11.64 ± 0.16 MPa) were observed in Group 2 (Bromelain). However, the maximum values of marginal leakage (37.21 ± 0.21) and minimum SBS (7.34 ± 0.07 MPa) were detected in Group 1 samples. The length of resin tags in the Group 2 specimens (Bromelain) was found to be the highest (102.11 ± 5.12 μm)^. However, Group 1 (No modifier) exhibited the lowest resin tag length (50.45 ± 2.37 μm).</p><p><strong>Conclusion: </strong>Caries-affected dentin modified with Bromelain resulted in satisfactory SBS with minimal microleakage scores. Chitosan nanoparticles and RFP/Ultraviolet-A also presented better outcomes in terms of microleakage and SBS than the control.</p>","PeriodicalId":94170,"journal":{"name":"Photodiagnosis and photodynamic therapy","volume":" ","pages":"104419"},"PeriodicalIF":0.0,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142711222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pigmented pathological type and depth of follicular extension as predictors of treatment failure in 5-aminolevulinic acid photodynamic therapy for actinic keratosis: A retrospective, matched nested case-control study.","authors":"Qinyuan Zhu, Huyan Chen, Jing Luan, Qiong Huang, Lianjun Chen, Wenyu Wu, Shujun Chen","doi":"10.1016/j.pdpdt.2024.104409","DOIUrl":"https://doi.org/10.1016/j.pdpdt.2024.104409","url":null,"abstract":"<p><strong>Background: </strong>While 5-Aminolevulinic acid photodynamic therapy (ALA-PDT) is widely used to treat actinic keratosis (AK), treatment resistance and recurrence after ALA-PDT remain significant clinical challenges.</p><p><strong>Methods: </strong>This single-site, retrospective, matched case-control study included 119 patients with histologically confirmed AK to identify clinical and pathological predictors for effectiveness of ALA-PDT. Patients received four consecutive ALA-PDT sessions at intervals of 1 or 2 weeks. Initial complete clearance (ICC) at 3 months and sustained complete clearance (SCC) at 12 months were assessed. Case patients were those with treatment-resistant or recurrent AK, matched with controls based on age, sex, and the treatment date. Baseline characteristics were collected and compared between the case and control groups.</p><p><strong>Results: </strong>ICC at 3 months was achieved in 106 out of 119 patients (89.07%), with 65 out of 82 patients (79.27%) maintaining SCC at 12 months. Pigmented AK emerged as an independent predictor of treatment resistance (OR=44.05, p=0.00). Furthermore, follicular extension to the isthmus or deeper was significantly associated with recurrence within 1 year (OR=17.26, p=0.00).</p><p><strong>Conclusion: </strong>Pigmented AK and AK with follicular extension to the isthmus or deeper may serve as independent predictors of treatment resistance and recurrence, respectively. These findings highlight the importance of these specific features when assessing prognosis and tailoring treatment strategies for individual AK patients.</p>","PeriodicalId":94170,"journal":{"name":"Photodiagnosis and photodynamic therapy","volume":" ","pages":"104409"},"PeriodicalIF":0.0,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142695829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combination treatment with photodynamic therapy and antibiotic in refractory cutaneous nocardiosis: A case report.","authors":"Xiaohan Liu, Yang Tan, Qionghui Cheng, Liang Wang, Jinyi Chen, Hong Liu, Wen Ding, Xia Lei","doi":"10.1016/j.pdpdt.2024.104414","DOIUrl":"10.1016/j.pdpdt.2024.104414","url":null,"abstract":"<p><strong>Background: </strong>Effective management of cutaneous nocardiosis is hampered by the lack of defined treatment recommendations and frequently requires prolonged antibiotic therapy.</p><p><strong>Case presentation: </strong>This report describes a case of cutaneous nocardiosis who was effectively treated in a brief amount of time with three sessions of 5-aminolevulinic acid photodynamic therapy (ALA-PDT) in conjunction with antibiotics.</p><p><strong>Conclusion: </strong>ALA-PDT combined with antibiotics may effectively treat refractory cutaneous nocardiosis.</p>","PeriodicalId":94170,"journal":{"name":"Photodiagnosis and photodynamic therapy","volume":" ","pages":"104414"},"PeriodicalIF":0.0,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142695371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}