MedComm最新文献

{"title":"Anoikis in cell fate, physiopathology, and therapeutic interventions","authors":"Jie Mei,&nbsp;Xue-Yao Jiang,&nbsp;Hui-Xiang Tian,&nbsp;Ding-Chao Rong,&nbsp;Jia-Nan Song,&nbsp;Luozixian Wang,&nbsp;Yuan-Shen Chen,&nbsp;Raymond C. B. Wong,&nbsp;Cheng-Xian Guo,&nbsp;Lian-Sheng Wang,&nbsp;Lei-Yun Wang,&nbsp;Peng-Yuan Wang,&nbsp;Ji-Ye Yin","doi":"10.1002/mco2.718","DOIUrl":"https://doi.org/10.1002/mco2.718","url":null,"abstract":"<p>The extracellular matrix (ECM) governs a wide spectrum of cellular fate processes, with a particular emphasis on anoikis, an integrin-dependent form of cell death. Currently, anoikis is defined as an intrinsic apoptosis. In contrast to traditional apoptosis and necroptosis, integrin correlates ECM signaling with intracellular signaling cascades, describing the full process of anoikis. However, anoikis is frequently overlooked in physiological and pathological processes as well as traditional in vitro research models. In this review, we summarized the role of anoikis in physiological and pathological processes, spanning embryonic development, organ development, tissue repair, inflammatory responses, cardiovascular diseases, tumor metastasis, and so on. Similarly, in the realm of stem cell research focused on the functional evolution of cells, anoikis offers a potential solution to various challenges, including in vitro cell culture models, stem cell therapy, cell transplantation, and engineering applications, which are largely based on the regulation of cell fate by anoikis. More importantly, the regulatory mechanisms of anoikis based on molecular processes and ECM signaling will provide new strategies for therapeutic interventions (drug therapy and cell-based therapy) in disease. In summary, this review provides a systematic elaboration of anoikis, thus shedding light on its future research.</p>","PeriodicalId":94133,"journal":{"name":"MedComm","volume":null,"pages":null},"PeriodicalIF":10.7,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mco2.718","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142233995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interleukin-12 sustained release system promotes hematopoietic recovery after radiation injury","authors":"Chuanchuan Lin,&nbsp;Yang Xiang,&nbsp;Yangyang Zhang,&nbsp;Zhenxing Yang,&nbsp;Nanxi Chen,&nbsp;Weiwei Zhang,&nbsp;Lanyue Hu,&nbsp;Jianxin Chen,&nbsp;Ya Luo,&nbsp;Xueying Wang,&nbsp;Yanni Xiao,&nbsp;Qing Zhang,&nbsp;Xi Ran,&nbsp;Li Chen,&nbsp;Jigang Dai,&nbsp;Zhongjun Li,&nbsp;Qian Ran","doi":"10.1002/mco2.704","DOIUrl":"https://doi.org/10.1002/mco2.704","url":null,"abstract":"<p>The continuous production of mature blood cell lineages is maintained by hematopoietic stem cells but they are highly susceptible to damage by ionizing radiation (IR) that induces death. Thus, devising therapeutic strategies that can mitigate hematopoietic toxicity caused by IR would benefit acute radiation syndrome (ARS) victims and patients receiving radiotherapy. Herein, we describe the preparation of an injectable hydrogel formulation based on Arg-Gly-Asp-alginate (RGD-Alg) and Laponite using a simple mixing method that ensured a slow and sustained release of interleukin-12 (IL-12) (RGD-Alg/Laponite@IL-12). The local administration of RGD-Alg/Laponite@IL-12 increased survival rates and promoted the hematopoietic recovery of mice who had received sublethal-dose irradiation. Local intra-bone marrow (intra-BM) injection of RGD-Alg/Laponite@IL-12 hydrogel effectively stimulated IL12 receptor-phosphoinositide 3-kinase/protein kinase B (IL-12R-PI3K/AKT) signaling axis, which promoted proliferation and hematopoietic growth factors secretion of BM mesenchymal stem/stromal cells. This signaling axis facilitates the repair of the hematopoietic microenvironment and plays a pivotal role in hematopoietic reconstitution. In conclusion, we describe a biomaterial-sustained release of IL-12 for the treatment of irradiated hematopoietic injury and provide a new therapeutic strategy for hematopoietic ARS.</p>","PeriodicalId":94133,"journal":{"name":"MedComm","volume":null,"pages":null},"PeriodicalIF":10.7,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mco2.704","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142174288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of ACSL4 in modulating farnesoid X receptor expression and M2 macrophage polarization in HBV-induced hepatocellular carcinoma","authors":"Wenbiao Chen,&nbsp;Huixuan Xu,&nbsp;Liliangzi Guo,&nbsp;Fengping Zheng,&nbsp;Jun Yao,&nbsp;Lisheng Wang","doi":"10.1002/mco2.706","DOIUrl":"https://doi.org/10.1002/mco2.706","url":null,"abstract":"<p>The intricate relationship between bile acid (BA) metabolism, M2 macrophage polarization, and hepatitis B virus-hepatocellular carcinoma (HBV-HCC) necessitates a thorough investigation of ACSL4's (acyl-CoA synthetase long-chain family member 4) role. This study combines advanced bioinformatics and experimental methods to elucidate ACSL4's significance in HBV-HCC development. Using bioinformatics, we identified differentially expressed genes in HBV-HCC. STRING and gene set enrichment analysis analyses were employed to pinpoint critical genes and pathways. Immunoinfiltration analysis, along with in vitro and in vivo experiments, assessed M2 macrophage polarization and related factors. ACSL4 emerged as a pivotal gene influencing HBV-HCC. In HBV-HCC liver tissues, ACSL4 exhibited upregulation, along with increased levels of M2 macrophage markers and BA. Silencing ACSL4 led to heightened farnesoid X receptor (FXR) expression, reduced BA levels, and hindered M2 macrophage polarization, thereby improving HBV-HCC conditions. This study underscores ACSL4's significant role in HBV-HCC progression. ACSL4 modulates BA-mediated M2 macrophage polarization and FXR expression, shedding light on potential therapeutic targets and novel insights into HBV-HCC pathogenesis.</p>","PeriodicalId":94133,"journal":{"name":"MedComm","volume":null,"pages":null},"PeriodicalIF":10.7,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mco2.706","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142174289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adenoid cystic carcinoma: insights from molecular characterization and therapeutic advances","authors":"Yunxuan Jia,&nbsp;Yupeng Liu,&nbsp;Haitang Yang,&nbsp;Feng Yao","doi":"10.1002/mco2.734","DOIUrl":"https://doi.org/10.1002/mco2.734","url":null,"abstract":"<p>Adenoid cystic carcinoma (ACC) is a malignant tumor primarily originating from the salivary glands, capable of affecting multiple organs. Although ACC typically exhibits slow growth, it is notorious for its propensity for neural invasion, local recurrence, and distant metastasis, making it a particularly challenging cancer to treat. The complexity of ACC's histological and molecular features poses significant challenges to current treatment modalities, which often show limited effectiveness. Recent advancements in single-cell RNA-sequencing (scRNA-seq) have begun to unravel unprecedented insights into the heterogeneity and subpopulation diversity within ACC, revealing distinct cellular phenotypes and origins. This review delves into the intricate pathological and molecular characteristics of ACC, focusing on recent therapeutic advancements. We particularly emphasize the insights gained from scRNA-seq studies that shed light on the cellular landscape of ACC, underscoring its heterogeneity and pathobiology. Moreover, by integrating analyses from public databases, this review proposes novel perspectives for advancing treatment strategies in ACC. This review contributes to the academic understanding of ACC by proposing novel therapeutic approaches informed by cutting-edge molecular insights, paving the way for more effective, personalized therapeutic approaches for this challenging malignancy.</p>","PeriodicalId":94133,"journal":{"name":"MedComm","volume":null,"pages":null},"PeriodicalIF":10.7,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mco2.734","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142170199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Loss-of-function variants in RNA binding motif protein X-linked induce neuronal defects contributing to amyotrophic lateral sclerosis pathogenesis","authors":"Di He,&nbsp;Xinyi He,&nbsp;Dongchao Shen,&nbsp;Liyang Liu,&nbsp;Xunzhe Yang,&nbsp;Meng Hao,&nbsp;Yi Wang,&nbsp;Yi Li,&nbsp;Qing Liu,&nbsp;Mingsheng Liu,&nbsp;Jiucun Wang,&nbsp;Xue Zhang,&nbsp;Liying Cui","doi":"10.1002/mco2.712","DOIUrl":"https://doi.org/10.1002/mco2.712","url":null,"abstract":"<p>Despite being one of the most prevalent RNA modifications, the role of N6-methyladenosine (m6A) in amyotrophic lateral sclerosis (ALS) remains ambiguous. In this investigation, we explore the contribution of genetic defects of m6A-related genes to ALS pathogenesis. We scrutinized the mutation landscape of m6A genes through a comprehensive analysis of whole-exome sequencing cohorts, encompassing 508 ALS patients and 1660 population-matched controls. Our findings reveal a noteworthy enrichment of RNA binding motif protein X-linked (<i>RBMX</i>) variants among ALS patients, with a significant correlation between pathogenic m6A variants and adverse clinical outcomes. Furthermore, <i>Rbmx</i> knockdown in NSC-34 cells overexpressing mutant TDP43^Q331K results in cell death mediated by an augmented p53 response. Similarly, <i>RBMX</i> knockdown in ALS motor neurons derived from induced pluripotent stem cells (iPSCs) manifests morphological defects and activation of the p53 pathway. Transcriptional analysis using publicly available single-cell sequencing data from the primary motor cortex indicates that RBMX-regulated genes selectively influence excitatory neurons and exhibit enrichment in ALS-implicated pathways. Through integrated analyses, our study underscores the emerging roles played by <i>RBMX</i> in ALS, suggesting a potential nexus between the disease and dysregulated m6A-mediated mRNA metabolism.</p>","PeriodicalId":94133,"journal":{"name":"MedComm","volume":null,"pages":null},"PeriodicalIF":10.7,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mco2.712","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142169853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial properties of natural cinnamon-alginate fibrous patches produced by modified nozzle-pressurized spinning","authors":"Yanqi Dai,&nbsp;Merve Gultekinoglu,&nbsp;Cem Bayram,&nbsp;Hettiyahandi Binodh De Silva,&nbsp;Mohan Edirisinghe","doi":"10.1002/mco2.731","DOIUrl":"https://doi.org/10.1002/mco2.731","url":null,"abstract":"&lt;p&gt;Dear Editor,&lt;/p&gt;&lt;p&gt;Alginate (Alg) is of particular interest as a natural biomaterial due to its unique gelling properties and water absorption capacity. Despite these advantages, the transformation of Alg into commercially value-added products still faces many challenges.&lt;span&gt;&lt;sup&gt;1&lt;/sup&gt;&lt;/span&gt; Our recent study investigates an advanced spinning technology for the facile and large-scale production of small-structure Alg antibacterial natural patches incorporated with Ceylon cinnamon.&lt;/p&gt;&lt;p&gt;In this work, nozzle-pressurized spinning (NPS)&lt;span&gt;&lt;sup&gt;2&lt;/sup&gt;&lt;/span&gt; functioned as a jet generation apparatus (Figure S1) spinning Na-Alg jets into a Ca&lt;sup&gt;2+&lt;/sup&gt;-riched coagulation bath. Ca&lt;sup&gt;2+&lt;/sup&gt; combined with Alg chains in the cross-linked “egg-box” model to form Alg fibers. Na-Alg solutions were generally significantly viscous even at a relatively low concentration (&lt; 5 wt%), exhibiting a pronounced solid-like behavior. The high pressure applied in NPS effectively mitigates these viscous effects, facilitating Na-Alg jet formation. Additionally, given the significant production efficiency of NPS, this strategy stands out as a promising approach for the scaling up of Alg fiber production, compared with prevailing methods like electrospinning and wet spinning.&lt;/p&gt;&lt;p&gt;Figure 1A illustrates a marked alteration in the morphology of the obtained Alg products correlating with changes in Na-Alg/H&lt;sub&gt;2&lt;/sub&gt;O solution concentration in NPS. As the concentration increased, the Alg morphology evolved from a thin film to a ribbon-like structure, ultimately obtaining a filamentous form at the concentration of 3.0 wt%. The rapid increase of solution viscosity with its increasing concentration is a remarkable feature of Na-Alg/H&lt;sub&gt;2&lt;/sub&gt;O solution, accompanied by a significant reduction in its fluidity.&lt;span&gt;&lt;sup&gt;3&lt;/sup&gt;&lt;/span&gt; Thereby, the morphology of the resulting Alg products varied. Meeting the critical rheological properties is the key to producing Alg fibers with well-defined filamentous structures (Figure 1A). Weighing the synergistic effect of solution properties and system parameters, we successfully produced Alg fibers with an average diameter of 10 µm using 3.2 wt% Na-Alg/H&lt;sub&gt;2&lt;/sub&gt;O solution.&lt;/p&gt;&lt;p&gt;Following the established correlation between Alg products and solution properties/processing parameters, Ceylon cinnamon (grounded cinnamon, GC; supplied by HDDES Extracts [PVT] Ltd) was incorporated into Alg fibers using NPS to generate Alg-GC fibrous patches, with weight ratios of GC of 1%, 2%, and 4% (Alg-GC1, Alg-GC2, and, Alg-GC4). The potential of the resulting Alg-GC fibrous patches as a biomaterial candidate was evaluated in terms of in-vitro cell viability and antibacterial properties.&lt;/p&gt;&lt;p&gt;Indirect cytotoxicity tests of pure Alg fibers and Alg-GC patches were performed by WST-1 assay according to ISO10993-5 standard for medical devices.&lt;span&gt;&lt;sup&gt;4&lt;/sup&gt;&lt;/span&gt; The results proved that Alg is a biocompatible biomaterial and fibro","PeriodicalId":94133,"journal":{"name":"MedComm","volume":null,"pages":null},"PeriodicalIF":10.7,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mco2.731","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142169854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Krüppel-like factors family in health and disease","authors":"Tingwen Xiang,&nbsp;Chuan Yang,&nbsp;Zihan Deng,&nbsp;Dong Sun,&nbsp;Fei Luo,&nbsp;Yueqi Chen","doi":"10.1002/mco2.723","DOIUrl":"https://doi.org/10.1002/mco2.723","url":null,"abstract":"<p>Krüppel-like factors (KLFs) are a family of basic transcription factors with three conserved Cys2/His2 zinc finger domains located in their C-terminal regions. It is acknowledged that KLFs exert complicated effects on cell proliferation, differentiation, survival, and responses to stimuli. Dysregulation of KLFs is associated with a range of diseases including cardiovascular disorders, metabolic diseases, autoimmune conditions, cancer, and neurodegenerative diseases. Their multidimensional roles in modulating critical pathways underscore the significance in both physiological and pathological contexts. Recent research also emphasizes their crucial involvement and complex interplay in the skeletal system. Despite the substantial progress in understanding KLFs and their roles in various cellular processes, several research gaps remain. Here, we elucidated the multifaceted capabilities of KLFs on body health and diseases via various compliable signaling pathways. The associations between KLFs and cellular energy metabolism and epigenetic modification during bone reconstruction have also been summarized. This review helps us better understand the coupling effects and their pivotal functions in multiple systems and detailed mechanisms of bone remodeling and develop potential therapeutic strategies for the clinical treatment of pathological diseases by targeting the KLF family.</p>","PeriodicalId":94133,"journal":{"name":"MedComm","volume":null,"pages":null},"PeriodicalIF":10.7,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mco2.723","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142169852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural insight into interleukin-4Rα and interleukin-5 inhibition by nanobodies from a bispecific antibody","authors":"Weicheng Qiu,&nbsp;Jinguo Meng,&nbsp;Zhipeng Su,&nbsp;Wei Xie,&nbsp;Gaojie Song","doi":"10.1002/mco2.700","DOIUrl":"https://doi.org/10.1002/mco2.700","url":null,"abstract":"&lt;p&gt;Dear Editor,&lt;/p&gt;&lt;p&gt;Specific immunological challenges can stimulate T helper 2 (Th2) cells and trigger immune response by secreting cytokines such as interleukin-4 (IL-4), IL-5, and IL-13, and dysregulation of these cytokines are closely related to the pathogenesis of diseases such as atopic dermatitis and allergic asthma.&lt;span&gt;&lt;sup&gt;1&lt;/sup&gt;&lt;/span&gt; At the molecular level, these cytokines function by binding to different cytokine receptors, thus triggering various inflammatory responses. IL-4 can either bind individually via a heterodimeric receptor composed of IL-4Rα(CD124) and γc (CD132) to trigger a type I inflammatory response or signal through a shared type II inflammatory response with IL-13 to bind another heterodimeric receptor composed of IL-4Rα and IL-13α1. Both types of receptor complexes can activate the phosphorylation of the signal transducer and activator of the transcription (STAT6) pathway via Janus kinase. In addition, IL-5 functions via the IL-5 receptor, which is also a heterodimer consisting of a specific α subunit for binding (IL-5Rα) and a shared β subunit for signal transduction (colony-stimulating factor 2 receptor beta, CSF2RB). Currently, several monoclonal antibody drugs are approved for marketing worldwide for asthma indications, but these are mainly single-target blockers related to IL-4, IL-5, and IL-13 signaling pathways,&lt;span&gt;&lt;sup&gt;1&lt;/sup&gt;&lt;/span&gt; and most of these medicines have individual limitations. According to investigations of Th2 cell-related signaling pathways and the outcomes of some clinical trials,&lt;span&gt;&lt;sup&gt;2&lt;/sup&gt;&lt;/span&gt; blocking different steps within the type II inflammatory pathway may produce better efficacy as this approach is expected to produce synergistic effects.&lt;/p&gt;&lt;p&gt;Here, we attempted to develop a bispecific antibody targeting both IL-4Rα and IL-5 within the type II inflammatory pathway. To obtain the VHHs, we immunized alpaca with individual proteins of IL-4Rα or IL-5 (Figure 1A). Taking IL-4Rα for example, total RNA was extracted from lymphocytes to construct a VHH library. 109 unique binders for IL4Rα were identified after library screening. After validating binding and blocking capacity, we selected two binders (dAb1 and dAb2) for further engineering and humanization. These two nanobodies exhibited similar EC&lt;sub&gt;50&lt;/sub&gt; (single digit nanomolar) when associated with IL-4Rα in either VHH or Fc-fused form, with dAb1 performing slightly better when blocking the IL-4&lt;b&gt;–&lt;/b&gt;IL-4Rα interaction (Figure S1A–D). We further found that dAb1 shows an IC&lt;sub&gt;50&lt;/sub&gt; of ∼0.15 nM in either IL-4 or IL-13-induced TF-1 cell proliferation assay (Figure 1B), which is comparable with the efficacy of dupilumab (Figure S1E,F). Subsequently, dAb1 was selected and recombined with the best VHH against IL-5 (which was generated and selected using a similar process) to produce a bispecific antibody.&lt;/p&gt;&lt;p&gt;Interestingly, we found the IL4Rα-binding nanobody dAb1 binds only to human IL-4Rα but has no cross-reactiv","PeriodicalId":94133,"journal":{"name":"MedComm","volume":null,"pages":null},"PeriodicalIF":10.7,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mco2.700","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142158574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Radiogenomics: bridging the gap between imaging and genomics for precision oncology","authors":"Wenle He,&nbsp;Wenhui Huang,&nbsp;Lu Zhang,&nbsp;Xuewei Wu,&nbsp;Shuixing Zhang,&nbsp;Bin Zhang","doi":"10.1002/mco2.722","DOIUrl":"https://doi.org/10.1002/mco2.722","url":null,"abstract":"<p>Genomics allows the tracing of origin and evolution of cancer at molecular scale and underpin modern cancer diagnosis and treatment systems. Yet, molecular biomarker-guided clinical decision-making encounters major challenges in the realm of individualized medicine, consisting of the invasiveness of procedures and the sampling errors due to high tumor heterogeneity. By contrast, medical imaging enables noninvasive and global characterization of tumors at a low cost. In recent years, radiomics has overcomes the limitations of human visual evaluation by high-throughput quantitative analysis, enabling the comprehensive utilization of the vast amount of information underlying radiological images. The cross-scale integration of radiomics and genomics (hereafter radiogenomics) has the enormous potential to enhance cancer decoding and act as a catalyst for digital precision medicine. Herein, we provide a comprehensive overview of the current framework and potential clinical applications of radiogenomics in patient care. We also highlight recent research advances to illustrate how radiogenomics can address common clinical problems in solid tumors such as breast cancer, lung cancer, and glioma. Finally, we analyze existing literature to outline challenges and propose solutions, while also identifying future research pathways. We believe that the perspectives shared in this survey will provide a valuable guide for researchers in the realm of radiogenomics aiming to advance precision oncology.</p>","PeriodicalId":94133,"journal":{"name":"MedComm","volume":null,"pages":null},"PeriodicalIF":10.7,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mco2.722","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142158576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Leveraging single-cell RNA-seq for uncovering naïve B cells associated with better prognosis of hepatocellular carcinoma","authors":"Qingjia Sun,&nbsp;Rui Gao,&nbsp;Yingxin Lin,&nbsp;Xianchao Zhou,&nbsp;Tao Wang,&nbsp;Jian He","doi":"10.1002/mco2.563","DOIUrl":"https://doi.org/10.1002/mco2.563","url":null,"abstract":"<p>Hepatocellular carcinoma (HCC) is a typical highly heterogeneous solid tumor with high morbidity and mortality worldwide, especially in China; however, the immune microenvironment of HCC has not been clarified so far. Here, we employed single-cell RNA sequencing (scRNA-seq) on diethylnitrosamine (DEN)-induced mouse HCC model to dissect the immune cell dynamics during tumorigenesis. Our findings reveal distinct immune profiles in both precancerous and cancerous lesions, indicating early tumor-associated immunological alterations. Notably, specific T and B cell subpopulations are preferentially enriched in the HCC tumor microenvironment (TME). Furthermore, we identified a subpopulation of naïve B cells with high CD83 expression, correlating with improved prognosis in human HCC. These signature genes were validated in The Cancer Genome Atlas HCC RNA-seq dataset. Moreover, cell interaction analysis revealed that subpopulations of B cells in both mouse and human samples are activated and may potentially contribute to oncogenic processes. In summary, our study provides insights into the dynamic immune microenvironment and cellular networks in HCC pathogenesis, with a specific emphasis on naïve B cells. These findings emphasize the significance of targeting TME in HCC patients to prevent HCC pathological progression, which may give a new perspective on the therapeutics for HCC.</p>","PeriodicalId":94133,"journal":{"name":"MedComm","volume":null,"pages":null},"PeriodicalIF":10.7,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mco2.563","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142158575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}