Journal of the Chinese Medical Association : JCMA最新文献

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The influences of antihistamine on liver fibrosis, vasoresponsiveness and portosystemic shunting in bile duct-ligated cirrhotic rats. 抗组胺对胆管结扎型肝硬化大鼠肝纤维化、血管反应性及门系统分流的影响。
IF 2.4
Journal of the Chinese Medical Association : JCMA Pub Date : 2025-10-09 DOI: 10.1097/JCMA.0000000000001301
Chien-Lin Chang, Ching-Chih Chang, Chiao-Lin Chuang, Shao-Jung Hsu, Ming-Hung Tsai, Chia-Jui Tsai, Yu-Yin Chang, Ming-Chih Hou, Fa-Yauh Lee
{"title":"The influences of antihistamine on liver fibrosis, vasoresponsiveness and portosystemic shunting in bile duct-ligated cirrhotic rats.","authors":"Chien-Lin Chang, Ching-Chih Chang, Chiao-Lin Chuang, Shao-Jung Hsu, Ming-Hung Tsai, Chia-Jui Tsai, Yu-Yin Chang, Ming-Chih Hou, Fa-Yauh Lee","doi":"10.1097/JCMA.0000000000001301","DOIUrl":"https://doi.org/10.1097/JCMA.0000000000001301","url":null,"abstract":"<p><strong>Background: </strong>Liver cirrhosis with portal hypertension is a sequelae of chronic hepatitis, characterized by liver fibrosis, poor portosystemic collateral vasoresponsiveness, increased mesenteric angiogenesis and collaterals formation. Antihistamines are frequently used to control pruritus in cirrhotic patients, and it has also been shown to reverse histamine-induced inflammation, fibrogenesis, angiogenesis, and vasodilatation. However, the relevant impacts on cirrhosis are not clear.</p><p><strong>Methods: </strong>Liver cirrhosis was induced in rats with common bile duct ligation (BDL). Sham rats were surgical controls. Levocetirizine (0.5 mg/kg/day, oral gavage) or vehicle was administered from the 1st day after operations and experiments were performed on the 29th day to survey: (a) Systemic and splanchnic hemodynamic parameters; (b) Serum liver and renal biochemistry parameters; (c) Protein expressions of liver fibrogenesis factors; (d) Portosystemic collateral vasoresponsiveness to vasopressin; (e) Portosystemic shunting degree; and (f) Mesenteric angiogenesis.</p><p><strong>Results: </strong>Compared with vehicle, levocetirizine decreased portal pressure, superior mesenteric artery flow, portal vein (PV) flow and elevated PV resistance in BDL rats. Levocetirizine significantly down-regulated the hepatic TGF-β, p-Smad2, MMP-2, TIMP-1 protein expressions in BDL rats. Levocetirizine did not affect the collateral vasoresponsiveness to vasopressin but tended to reduce the severity of portosystemic shunting and mesenteric angiogenesis.</p><p><strong>Conclusion: </strong>Levocetirizine ameliorated liver fibrosis, portal hypertension and splanchnic hyperdynamic circulation in cirrhotic rats, possibly through the down-regulation of hepatic fibrogenesis factors. Antihistamines may be a therapeutic option to control portal hypertension. However, further investigations are required to clarify its clinical application.</p>","PeriodicalId":94115,"journal":{"name":"Journal of the Chinese Medical Association : JCMA","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145254273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Hemodialysis vascular access flow surveillance: Current evidence and future directions. 血液透析血管通路流量监测:目前的证据和未来的方向。
IF 2.4
Journal of the Chinese Medical Association : JCMA Pub Date : 2025-10-07 DOI: 10.1097/JCMA.0000000000001302
Tz-Heng Chen, Yan-Ting Shiu, Timmy Lee, Chih-Yu Yang, Der-Cherng Tarng
{"title":"Hemodialysis vascular access flow surveillance: Current evidence and future directions.","authors":"Tz-Heng Chen, Yan-Ting Shiu, Timmy Lee, Chih-Yu Yang, Der-Cherng Tarng","doi":"10.1097/JCMA.0000000000001302","DOIUrl":"https://doi.org/10.1097/JCMA.0000000000001302","url":null,"abstract":"<p><p>A well-functioning vascular access is essential for delivering adequate hemodialysis in patients with end-stage renal disease. However, vascular access dysfunction, particularly stenosis and thrombosis, remains a leading cause of morbidity, repeated interventions, and hospitalization in this patient population. Vascular access monitoring and surveillance are designed to detect hemodynamically significant stenosis early, thereby reducing the risk of thrombosis and maintaining access patency. Evidence from meta-analyses and randomized controlled trials suggests that access blood flow (Qa)-based surveillance may lower thrombosis rates in arteriovenous fistulas (AVFs), while the benefit appears less consistent for arteriovenous grafts (AVGs). Consequently, most guidelines recommended incorporating Qa surveillance into routine clinical monitoring for AVFs, but not as a standard practice for AVGs. However, previous studies have notable limitations, including heterogeneous surveillance protocols and variable definitions of access dysfunction. More rigorously designed randomized controlled trials are needed to clarify the role of Qa surveillance and inform optimal strategies. Looking ahead, emerging technologies such as artificial intelligence and wearable devices for continuous monitoring hold promise for enhancing diagnostic accuracy, enabling earlier detection of dysfunction, and reducing the need for intervention rates. Integrating these innovations with standardized surveillance protocols and individualized patient risk stratification has the potential to improve vascular access longevity, reduce the healthcare burden, and improve outcomes in the hemodialysis population, although further validation is required.</p>","PeriodicalId":94115,"journal":{"name":"Journal of the Chinese Medical Association : JCMA","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145254258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Waist circumference is an important determinant of relative muscle strength in patients with type 2 diabetes mellitus. 腰围是2型糖尿病患者相对肌力的重要决定因素。
IF 2.4
Journal of the Chinese Medical Association : JCMA Pub Date : 2025-10-01 Epub Date: 2025-09-03 DOI: 10.1097/JCMA.0000000000001288
Yi-Lun Chiang, Guan-Yu Su, Shiow-Chwen Tsai, Chiao-Nan Chen, Chii-Min Hwu, Tsung-Hui Wu
{"title":"Waist circumference is an important determinant of relative muscle strength in patients with type 2 diabetes mellitus.","authors":"Yi-Lun Chiang, Guan-Yu Su, Shiow-Chwen Tsai, Chiao-Nan Chen, Chii-Min Hwu, Tsung-Hui Wu","doi":"10.1097/JCMA.0000000000001288","DOIUrl":"10.1097/JCMA.0000000000001288","url":null,"abstract":"<p><strong>Background: </strong>Muscle damage has recently been recognized as a complication of type 2 diabetes mellitus (T2DM). Impaired muscle function adversely affects physical activity, quality of life, and mortality risk. Moreover, waist circumference (WC) is associated with visceral fat and insulin resistance. This study investigated the correlation between relative muscle strength and WC in patients with T2DM.</p><p><strong>Methods: </strong>Patients aged 50 years or older with T2DM were recruited from Taipei Veterans General Hospital. WC was measured at the umbilicus level. Total muscle mass was assessed using bioelectrical impedance analysis. The muscle strength of handgrip, knee extension, ankle dorsiflexion, and ankle plantar flexion were evaluated using a hand-held dynamometer.</p><p><strong>Results: </strong>A total of 252 patients (mean age 69.4 years; 122 men and 130 women) were enrolled. They were stratified into three groups according to WC (<85, 85-94, >94 cm). Patients with a larger WC had a higher body mass index and greater muscle mass but had lower relative muscle strength of handgrip, knee extension, ankle dorsiflexion, and ankle plantar flexion. Of note, patients with abdominal obesity (WC ≥90 cm in men and WC ≥80 cm in women) had significantly lower relative muscle strength of handgrip and lower limb compared to those without in both sexes. Multivariable analyses revealed that WC was significantly and negatively correlated with relative muscle strength after adjusting for other clinical variables.</p><p><strong>Conclusion: </strong>WC, independent of muscle mass, is a good determinant of relative muscle strength in patients with T2DM. Measuring WC can help identify patients with T2DM at a high risk of low relative muscle strength, thereby broadening the scope of health screening in this population.</p>","PeriodicalId":94115,"journal":{"name":"Journal of the Chinese Medical Association : JCMA","volume":" ","pages":"767-773"},"PeriodicalIF":2.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144984168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Repetitive transcranial magnetic stimulation in spinocerebellar ataxia: A promising avenue amidst clinical trial challenges. 重复经颅磁刺激治疗脊髓小脑共济失调:在临床试验挑战中有希望的途径。
IF 2.4
Journal of the Chinese Medical Association : JCMA Pub Date : 2025-10-01 Epub Date: 2025-06-19 DOI: 10.1097/JCMA.0000000000001257
Jong-Ling Fuh
{"title":"Repetitive transcranial magnetic stimulation in spinocerebellar ataxia: A promising avenue amidst clinical trial challenges.","authors":"Jong-Ling Fuh","doi":"10.1097/JCMA.0000000000001257","DOIUrl":"10.1097/JCMA.0000000000001257","url":null,"abstract":"","PeriodicalId":94115,"journal":{"name":"Journal of the Chinese Medical Association : JCMA","volume":" ","pages":"733-734"},"PeriodicalIF":2.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144328243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Trendelenburg position decreases the threshold of dynamic variables in predicting fluid responsiveness: A prospective observational study. Trendelenburg位置降低了预测流体响应性的动态变量阈值:一项前瞻性观察研究。
IF 2.4
Journal of the Chinese Medical Association : JCMA Pub Date : 2025-10-01 Epub Date: 2025-08-25 DOI: 10.1097/JCMA.0000000000001284
Chih-Chung Wu, Eric Yi-Hsiu Huang, Hui-Hsuan Ke, Yu-Hsien Wang, Wen-Kuei Chang, Chien-Kun Ting
{"title":"The Trendelenburg position decreases the threshold of dynamic variables in predicting fluid responsiveness: A prospective observational study.","authors":"Chih-Chung Wu, Eric Yi-Hsiu Huang, Hui-Hsuan Ke, Yu-Hsien Wang, Wen-Kuei Chang, Chien-Kun Ting","doi":"10.1097/JCMA.0000000000001284","DOIUrl":"10.1097/JCMA.0000000000001284","url":null,"abstract":"<p><strong>Background: </strong>Goal-directed fluid therapy (GDFT) individualizes fluid administration to optimize oxygen delivery and tissue perfusion. Traditional static parameters, such as central venous and pulmonary artery wedge pressures, are inaccurate. Instead, GDFT employs dynamic variables like stroke volume variation (SVV) and pulse pressure variation (PPV) to assess preload responsiveness and guide fluid administration. However, the Trendelenburg position alters cardiovascular physiology, and the effects of different tilt angles on SVV, PPV, and other hemodynamic variables remain unclear.</p><p><strong>Methods: </strong>This single-center, observational study enrolled patients undergoing elective surgery. Following induction of general anesthesia, patients were positioned supine (0°) and then in the Trendelenburg position, with the angle adjusted to 5°, 10°, 15°, and 25°. Dynamic variables, including SVV, PPV, contractility (d P /d t ), dynamic arterial elastance (Eadyn), and cardiac index (CI), at each angle were recorded and analyzed.</p><p><strong>Results: </strong>A total of 27 patients (mean age 66.7 ± 11.7 years, 79% male) were included. Increasing the Trendelenburg tilt angle from 0° to 25° was associated with a significant reduction in SVV (12.21 ± 4.26% at 0° to 6.94 ± 2.37% at 25°, p < 0.001) and PPV (13.47 ± 4.27% at 0° to 6.81 ± 3.20% at 25°, p < 0.001). SVV and PPV measured in the supine position correlated significantly with values obtained at 5°, 10°, 15°, and 25° ( p < 0.001). d P /d t progressively declined from 711.85 ± 235.58 mmHg/s at 0° to 583.85 ± 166.19 mmHg/s at 25° ( p < 0.001), and CI decreased from 2.51 ± 0.59 L/min/m² to 2.27 ± 0.60 L/min/m² ( p = 0.001). Eadyn trended downward but did not reach statistical significance ( p = 0.088).</p><p><strong>Conclusion: </strong>The thresholds of SVV and PPV for predicting fluid responsiveness decrease with progressive Trendelenburg tilt. These variables should therefore be interpreted cautiously in relation to tilt angle when guiding fluid management.</p>","PeriodicalId":94115,"journal":{"name":"Journal of the Chinese Medical Association : JCMA","volume":" ","pages":"783-789"},"PeriodicalIF":2.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144984162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Outcomes of everolimus-treated HR+/HER2- breast cancer. 依维莫司治疗HR+/HER2-乳腺癌的结果。
IF 2.4
Journal of the Chinese Medical Association : JCMA Pub Date : 2025-10-01 Epub Date: 2025-08-25 DOI: 10.1097/JCMA.0000000000001282
Peng-Hui Wang, Szu-Ting Yang, Tsung-Cheng Kuo
{"title":"Outcomes of everolimus-treated HR+/HER2- breast cancer.","authors":"Peng-Hui Wang, Szu-Ting Yang, Tsung-Cheng Kuo","doi":"10.1097/JCMA.0000000000001282","DOIUrl":"10.1097/JCMA.0000000000001282","url":null,"abstract":"","PeriodicalId":94115,"journal":{"name":"Journal of the Chinese Medical Association : JCMA","volume":" ","pages":"730-732"},"PeriodicalIF":2.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144984149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparison of operative outcomes between proximal and total gastrectomy for proximal gastric cancer. 近端胃癌近端切除术与全胃切除术手术效果比较。
IF 2.4
Journal of the Chinese Medical Association : JCMA Pub Date : 2025-10-01 Epub Date: 2025-09-03 DOI: 10.1097/JCMA.0000000000001287
Chin-Yung Chen, Ching-Yun Kung, Bor-Shiuan Shyr, Kuo-Hung Huang, Wen-Liang Fang, Shih-Chieh Lin, Anna Fen-Yau Li, Su-Shun Lo, Chew-Wun Wu, Yuan-Tzu Lan
{"title":"Comparison of operative outcomes between proximal and total gastrectomy for proximal gastric cancer.","authors":"Chin-Yung Chen, Ching-Yun Kung, Bor-Shiuan Shyr, Kuo-Hung Huang, Wen-Liang Fang, Shih-Chieh Lin, Anna Fen-Yau Li, Su-Shun Lo, Chew-Wun Wu, Yuan-Tzu Lan","doi":"10.1097/JCMA.0000000000001287","DOIUrl":"10.1097/JCMA.0000000000001287","url":null,"abstract":"<p><strong>Background: </strong>Total gastrectomy and proximal gastrectomy (PG) are both treatment options for proximal gastric cancer. Currently, there is no consensus on which procedure is better. The aim of this study was to compare the operative outcomes between PG and total gastrectomy in the treatment of proximal gastric cancer.</p><p><strong>Methods: </strong>Between January 2000 and October 2022, patients who underwent either PG with double tract reconstruction (PG-DTR) or total gastrectomy with Roux-en-Y reconstruction (TG-RY) for proximal gastric cancer were included. The clinicopathologic characteristics and operative outcomes were compared between the two groups. Propensity score matching was performed to compare the short- and long-term outcomes between the two groups.</p><p><strong>Results: </strong>A total of 263 patients were included in the study. After propensity-score matching, there was no significant difference in clinicopathological characteristics between the two groups. The TG-RY group had more retrieved lymph nodes (37.8 ± 18.6 vs 28.7 ± 15.4; p = 0.022) and a longer hospital stay (13.6 ± 10.2 vs 9.4 ± 3.3 days; p = 0.036) than the PG-DTR group. Surgical complications were similar between the two groups. The PG-DTR group had a greater prevalence of reflux esophagitis (21.4% vs 7.1%; p = 0.034), a higher postoperative/preoperative body weight ratio (0.91 ± 0.08 vs 0.84 ± 0.14; p = 0.021), and a higher postoperative/preoperative serum albumin ratio (1.07 ± 0.11 vs 0.96 ± 0.18; p = 0.004) than the TG-RY group.</p><p><strong>Conclusion: </strong>Compared with TG-RY, PG-DTR was associated with better postoperative nutritional status and comparable operative complications.</p>","PeriodicalId":94115,"journal":{"name":"Journal of the Chinese Medical Association : JCMA","volume":" ","pages":"800-806"},"PeriodicalIF":2.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144984031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Incretin integration: Charting the future of cardiovascular-kidney-metabolic health. 肠促胰岛素整合:描绘心血管-肾脏-代谢(CKM)健康的未来
IF 2.4
Journal of the Chinese Medical Association : JCMA Pub Date : 2025-10-01 Epub Date: 2025-08-01 DOI: 10.1097/JCMA.0000000000001277
Chih-Yuan Wang
{"title":"Incretin integration: Charting the future of cardiovascular-kidney-metabolic health.","authors":"Chih-Yuan Wang","doi":"10.1097/JCMA.0000000000001277","DOIUrl":"10.1097/JCMA.0000000000001277","url":null,"abstract":"","PeriodicalId":94115,"journal":{"name":"Journal of the Chinese Medical Association : JCMA","volume":" ","pages":"728-729"},"PeriodicalIF":2.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144762884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evolving treatment and survival in hypopharyngeal cancer: A single-center study. 下咽癌的发展治疗和生存:一项单中心研究。
IF 2.4
Journal of the Chinese Medical Association : JCMA Pub Date : 2025-10-01 Epub Date: 2025-08-07 DOI: 10.1097/JCMA.0000000000001279
Chia-Fan Chang, Pen-Yuan Chu
{"title":"Evolving treatment and survival in hypopharyngeal cancer: A single-center study.","authors":"Chia-Fan Chang, Pen-Yuan Chu","doi":"10.1097/JCMA.0000000000001279","DOIUrl":"10.1097/JCMA.0000000000001279","url":null,"abstract":"<p><strong>Background: </strong>Patients with hypopharyngeal squamous cell carcinoma (HPSCC) often present with advanced disease, resulting in poor prognoses. Traditional treatment involves radical surgery, which affects the quality of life considerably. At present, treatment is shifting away from primary surgical therapy. However, the long-term adverse effect is notable. This study examined the treatment outcomes of patients with HPSCC at a single medical center to assess the effects of evolving treatment modalities on survival and laryngeal preservation.</p><p><strong>Methods: </strong>This retrospective study analyzed patients with HPSCC treated from 2004 to 2012. To assess the effects of changes in treatment modalities, the study period was divided into three blocks: 2004-2006, 2007-2009, and 2010-2012. Tumor recurrence, functional laryngeal outcomes, and survival rates were assessed across the three periods.</p><p><strong>Results: </strong>A total of 167 patients with HPSCC (161 males and seven females; median age: 57 years) were analyzed. The proportion of patients undergoing surgery, specifically transoral laser microsurgery (TLM), increased over time. The laryngeal preservation rates increased from 14% to 41%, and the tumor recurrence rates reduced from 48% to 31% over the study period. Surgery-based treatment was associated with survival rates comparable to those of chemoradiotherapy, with 5-year overall survival and disease-specific survival rates increasing over time from 32% to 55% and from 37% to 73%, respectively.</p><p><strong>Conclusion: </strong>Advancements in surgical techniques, precise radiotherapy, chemotherapy regimens, and refined treatment protocols have significantly enhanced survival outcomes and laryngeal preservation rates for patients with HPSCC over time. TLM has emerged as an effective conservation surgery in patients with early-stage and select cases of advanced HPSCC, offering the benefit of preserving laryngeal function.</p>","PeriodicalId":94115,"journal":{"name":"Journal of the Chinese Medical Association : JCMA","volume":" ","pages":"760-766"},"PeriodicalIF":2.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144796572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Phenylalanyl-tRNA synthetase subunit beta downregulation by spi1 proto-oncogene modulates lung adenocarcinoma progression and immune microenvironment via mammalian target of rapamycin pathway. SPI1下调FARSB通过mTOR途径调控肺腺癌进展和免疫微环境。
IF 2.4
Journal of the Chinese Medical Association : JCMA Pub Date : 2025-10-01 Epub Date: 2025-09-03 DOI: 10.1097/JCMA.0000000000001286
Yiting Wang, Yifan Zhou, Shangwei Chen, Jianwei Huang, Chen Zhang, Shuping Huang, Yujia Pan, Xiaoyan Huang, Junqi Qin, Shenghua Lin
{"title":"Phenylalanyl-tRNA synthetase subunit beta downregulation by spi1 proto-oncogene modulates lung adenocarcinoma progression and immune microenvironment via mammalian target of rapamycin pathway.","authors":"Yiting Wang, Yifan Zhou, Shangwei Chen, Jianwei Huang, Chen Zhang, Shuping Huang, Yujia Pan, Xiaoyan Huang, Junqi Qin, Shenghua Lin","doi":"10.1097/JCMA.0000000000001286","DOIUrl":"10.1097/JCMA.0000000000001286","url":null,"abstract":"<p><strong>Background: </strong>Phenylalanyl-tRNA synthetase subunit beta (FARSB) is implicated in the progression of multiple cancers and represents a potential therapeutic target. However, its role in lung adenocarcinoma (LUAD) progression and the immune microenvironment remains poorly understood, warranting further investigation into its regulatory mechanisms.</p><p><strong>Methods: </strong>We conducted bioinformatics analyses to investigate the expression levels of FARSB in LUAD, identify enriched pathways, and assess its correlation with patient prognosis and CD8 + T cell infiltration. Bioinformatics analysis was also used to explore the transcriptional repression of FARSB by spi1 proto-oncogene (SPI1) and to validate the targeting relationship between SPI1 and FARSB. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was utilized to measure the mRNA expression of FARSB and SPI1, while western blot was used to detect the expression of FARSB, SPI1, programmed death-ligand 1 (PD-L1), and related signaling pathway proteins. Functional assays were performed, including CCK-8 assay for cell viability, EdU incorporation for cell proliferation, and flow cytometry for apoptosis analysis. Carboxyfluorescein succinimidyl ester (CFSE) staining was used to analyze CD8 + T cell proliferation, and flow cytometry was used to assess the expression of cytokines interferon-gamma (IFN-γ), Granzyme B (GZMB), and tumor necrosis factor-alpha (TNF-α).</p><p><strong>Results: </strong>FARSB expression was significantly upregulated in LUAD tissues and cells, and it inhibited CD8 + T cell infiltration. Mechanistically, FARSB activated the mammalian target of rapamycin (mTOR) signaling pathway, enhancing LUAD cell viability, proliferation, and anti-apoptotic capabilities, consequently promoting CD8 + T cell exhaustion. The transcription factor SPI1 repressed FARSB expression, thus inhibiting LUAD progression and promoting CD8 + T cell anti-tumor immunity.</p><p><strong>Conclusion: </strong>SPI1 downregulated FARSB expression through transcriptional repression, thereby blocking the mTOR signaling pathway and suppressing LUAD progression and promoting CD8 + T cell anti-tumor immunity.</p>","PeriodicalId":94115,"journal":{"name":"Journal of the Chinese Medical Association : JCMA","volume":" ","pages":"790-799"},"PeriodicalIF":2.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144984157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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