Cancer Cytopathology最新文献

{"title":"Cytopathology of follicular and oncocytic follicular thyroid neoplasms: A Bethesda System perspective","authors":"André Lametti MD,&nbsp;Fadi Brimo MD,&nbsp;Yonca Kanber MD,&nbsp;Derin Caglar MD,&nbsp;Manon Auger MD","doi":"10.1002/cncy.70016","DOIUrl":"https://doi.org/10.1002/cncy.70016","url":null,"abstract":"<p>The third edition of The Bethesda System for Reporting Thyroid Cytopathology includes category IV, follicular neoplasm (FN), which is used to classify fine-needle aspirates of thyroid nodules that may correspond to invasive follicular-derived neoplasia other than papillary thyroid carcinoma. This diagnosis is infrequently rendered, and may represent a challenge for pathologists. This review presents a practical approach to FN and its subtype oncocytic follicular neoplasm (OFN). First, minimal criteria for the diagnosis must be achieved, namely sufficient cellularity, architectural features consistent with neoplasia, and follicular cell or oncocytic cytomorphology. Second, select diagnoses that are common or important differential diagnoses for FN or OFN must be ruled out, via a combination of morphological findings and limited ancillary tests, when available. These include follicular nodular disease, parathyroid sampling, metastatic carcinoma, noninvasive follicular thyroid neoplasm with papillary-like nuclear features, medullary thyroid carcinoma, certain subtypes of papillary thyroid carcinoma, and lymphocytic thyroiditis. This approach should allow for a careful selection of cases where diagnostic thyroid lobectomy is an appropriate therapeutic modality.</p>","PeriodicalId":9410,"journal":{"name":"Cancer Cytopathology","volume":"133 5","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cncy.70016","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143865829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytology of cystic lesions of the pancreas: Practical insights, pearls, and pitfalls","authors":"M. Lisa Zhang MD,&nbsp;Martha B. Pitman MD","doi":"10.1002/cncy.70011","DOIUrl":"https://doi.org/10.1002/cncy.70011","url":null,"abstract":"<p>Pancreatic cyst fluid (PCF) specimens present significant interpretive challenges. Accurate preoperative diagnosis is essential for guiding patient management, as pancreatic cysts vary from benign to pre-malignant and malignant. Appropriate triage differentiates low-risk cysts requiring surveillance from high-risk cysts necessitating surgical resection, the latter of which have increased likelihood of progressing to or harboring invasive carcinoma. Optimal PCF assessment integrates radiological, cytological, biochemical, and molecular findings if available. Key biochemical markers such as carcinoembryonic antigen and glucose can improve the detection of neoplastic mucinous cysts. However, cytology remains the most specific modality for identifying high-risk cysts. Cytomorphologic interpretation is particularly challenging due to the scant cellularity and degenerative changes often present in these specimens. This review provides practical insights to improve the evaluation of pancreatic cysts, emphasizing the importance of a multidisciplinary approach and highlighting diagnostic pearls and common pitfalls to aid in accurate interpretation and optimal patient care.</p>","PeriodicalId":9410,"journal":{"name":"Cancer Cytopathology","volume":"133 5","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143846196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Introducing Cancer Cytopathology Practice Essentials: A new series of practical and up-to-date reviews","authors":"Michiya Nishino MD, PhD,&nbsp;Mauro Saieg MD, PhD, FIAC","doi":"10.1002/cncy.70010","DOIUrl":"https://doi.org/10.1002/cncy.70010","url":null,"abstract":"","PeriodicalId":9410,"journal":{"name":"Cancer Cytopathology","volume":"133 5","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143846197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytology-Radiology Correlation Series: Pancreatic cytopathology","authors":"Judy Trieu MD,&nbsp;Andrew Gilman MD,&nbsp;Katerina Konstantinoff MD,&nbsp;Maria D. Lozano MD,&nbsp;Mauro Saieg MD, PhD","doi":"10.1002/cncy.70012","DOIUrl":"https://doi.org/10.1002/cncy.70012","url":null,"abstract":"<p>The prevalence of pancreatic lesions has increased over the years because of an increase in accessibility to and the quality of cross-sectional imaging. This commentary describes the common non-neoplastic and neoplastic pancreatic lesions. The images in this commentary depict classic cross-sectional images, sonographic findings, and the cytopathologic diagnosis of each lesion. Most common non-neoplastic lesions include pseudocysts, autoimmune pancreatitis, and chronic pancreatitis. Most common neoplastic lesions include serous cystadenomas, intraductal papillary mucinous neoplasms, mucinous cystic neoplasms, solid pseudopapillary neoplasms, neuroendocrine tumors, pancreatic ductal adenocarcinoma, acinar cell carcinoma, and metastases to the pancreas. The aim of this Cytoimaging Correlation Series is to demonstrate the multidisciplinary involvement in the diagnosis of pancreatic pathology and to highlight main findings in the most common entities found in everyday practice.</p>","PeriodicalId":9410,"journal":{"name":"Cancer Cytopathology","volume":"133 5","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cncy.70012","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143836168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinicopathological and molecular characterization of non–small cell lung cancer with pericardial effusions","authors":"Weijie Ma MD,&nbsp;Nathalie J. Rodrigues Simoes MD,&nbsp;Peter P. Seery,&nbsp;Tianhong Li MD, PhD,&nbsp;Laura J. Tafe MD,&nbsp;Darcy A. Kerr MD,&nbsp;Xiaoying Liu MD","doi":"10.1002/cncy.70015","DOIUrl":"https://doi.org/10.1002/cncy.70015","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Cytological evaluation is essential for assessing pericardial effusions (PEs) in non–small cell lung cancer (NSCLC). This study retrospectively examined the clinicopathological, molecular, and prognostic characteristics of patients with NSCLC with PE.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Clinical data from 80 patients with NSCLC with PE treated at an academic center over the course of 15 years were reviewed. PE specimens were categorized according to the International System for Reporting Serous Fluid Cytopathology (ISRSFC). The analysis included patient demographics, molecular alterations, cytopathology, histology, and survival outcomes.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Of the 80 patients, 36 (45%) were female and 90% had stage IV disease. A smoking history was noted in 58 patients (72.5%), and 22 patients (27.5%) presented with tamponade. Lung adenocarcinoma predominated (87.5%). The ISRSFC categorized 25% of the specimens as negative for malignancy (NFM), 7.5% as atypia of undetermined significance (AUS), 3.75% as suspicious for malignancy (SFM), and 63.75% as malignant (MAL). Immunohistochemistry in 57 specimens identified thyroid transcription factor 1 (65%) as the most frequently positive marker. Molecular analysis revealed <i>p53</i> mutations (59.1%) as the most prevalent, followed by <i>KRAS</i> (34.1%) and <i>EGFR</i> (15.9%). Kaplan–Meier analysis showed significantly better survival for NFM patients than non-NFM patients (MAL, SFM, and AUS; <i>p</i> = .0036). Bloody PEs and tamponade were associated with worse outcomes. The immunotherapy group achieved the most prolonged survival among stage IV patients (9.07 months; <i>p</i> = .017). Cox regression confirmed cytology-negative status as an independent prognostic factor.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Cytological evaluation and ISRSFC classification are crucial for NSCLC-associated PEs. A multidisciplinary approach integrating cytology, immunohistochemistry, and molecular profiling is essential for optimal management and prognosis.</p>\u0000 </section>\u0000 </div>","PeriodicalId":9410,"journal":{"name":"Cancer Cytopathology","volume":"133 5","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143831193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of conventional and novel rotational FNA needles using conventional microscopy and image analysis to quantitatively assess yield","authors":"Mohammed Amer Swid MD,&nbsp;Alivia E. Shen,&nbsp;Amanda J. Young MS,&nbsp;Tariq Rahman MD,&nbsp;Sara E. Monaco MD","doi":"10.1002/cncy.70014","DOIUrl":"https://doi.org/10.1002/cncy.70014","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>There is increasing interest in designing new fine-needle aspiration (FNA) needles to maximize tissue acquisition. This study compares cytological preparations from a new rotating FNA needle (CytoCore) with conventional FNA (ConvFNA) using semiquantitative evaluation and quantitative image analysis (IA).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>FNA were performed on ex vivo tissue in quadruplicate for each needle type (ConvFNA and CytoCore), including different sizes (22 G and 25 G) and variable procedure time (5 and 20 s). The Nikon Elements (v5.41.02) was used to quantify the cellularity and size of the largest tissue fragment on cell blocks.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>A total of 96 cytology specimens were evaluated were evaluated from benign and malignant specimens. For both ConvFNA and CytoCore, a longer procedure time (20 s) tended to produce greater cellularity and larger tissue fragments in the cell block specimens for both needles when analyzed with image analysis and was statistically significant for the CytoCore needle (<i>p</i> &lt; .01). The ConvFNA tended to perform better with short procedure time. There was no statistically significant difference using different needle gauges.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>This study shows that IA can help to quantitatively evaluate sample cellularity in the cell blocks from specimens acquired with different needles. A longer procedure time tended to produce more cellular samples and larger tissue fragments in the cell block for both ConvFNA and CytoCore needles and was statistically significant for CytoCore. Additional larger studies, including those with true clinical cases, should be considered to evaluate the different needle types further.</p>\u0000 </section>\u0000 </div>","PeriodicalId":9410,"journal":{"name":"Cancer Cytopathology","volume":"133 5","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143809384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"How pro-inflammatory diets may create the right recipe for cancer","authors":"Bryn Nelson PhD,&nbsp;William Faquin MD, PhD","doi":"10.1002/cncy.70007","DOIUrl":"https://doi.org/10.1002/cncy.70007","url":null,"abstract":"&lt;p&gt;Over the past few decades, the phrase &lt;i&gt;heart-healthy diet&lt;/i&gt; has become ubiquitous in public health messages urging people to reduce their sodium and saturated fat intake to help to prevent heart disease. Although the science behind a cancer-conscious diet is not as clear-cut, research has identified some risk-lowering foods such as plant-based proteins and more dangerous foods such as processed meats.&lt;/p&gt;&lt;p&gt;Ongoing studies have investigated a range of potential oncogenic mechanisms. For many foods and food additives linked to cancer, however, “a common underlying mechanistic theme seems to be inflammation,” says Lorne Hofseth, PhD, professor and associate dean for research in the College of Pharmacy at the University of South Carolina in Columbia. Accordingly, more scientists are keying in on foods and additives that may either promote or resolve inflammation.&lt;/p&gt;&lt;p&gt;In one recent study, researchers conducted an extensive analysis of the lipidome, or the entire collection of lipid molecules, in tissue samples from 81 colorectal cancer tumors.&lt;span&gt;&lt;sup&gt;1&lt;/sup&gt;&lt;/span&gt; Compared to samples from healthy volunteers, the tumor samples revealed a significant pro-inflammatory signature that included multiple products of arachidonic acid, an omega-6 fatty acid that the body can derive from a separate omega-6 fatty acid called linoleic acid.&lt;/p&gt;&lt;p&gt;Conversely, the tumor samples rev-ealed a relative dearth of inflammation-quenching molecules. “Long story short, we think cancer, colon cancer in particular, is a chronic inflammatory disease,” says senior author Timothy Yeatman, MD, FACS, professor of surgery at the University of South Florida and associate center director for translational research and innovation at the Tampa General Hospital Cancer Institute. That inflammation, in turn, may cause immunosuppression that aids the development of tumor cells.&lt;/p&gt;&lt;p&gt;Dr Yeatman and his colleagues support the long-standing idea that cancer is like a “chronically inflamed, poorly healing wound.” Under that hypothesis, cancer is not only a genetic disease marked by mutations in tumor suppressor genes but also a metabolic one in which chronic inflammation and insufficient immune surveillance allow mutation-harboring cells to gain a critical foothold.&lt;/p&gt;&lt;p&gt;What is the link to diet? Multiple studies have characterized Western diets, such as those in the United States, as low in fiber but high in fat—especially in omega-6 fatty acids, which predominate in common food ingredients such as soybean, sunflower, safflower, corn, and other seed oils. Although omega-6, like its omega-3 counterpart, is considered an essential fatty acid, Dr Yeatman says that the cancer connection may be due to a wild overabundance of the former compared to the latter in what we eat. “Consequently, our ratios of omega-6 to omega-3 are out of whack,” he says. “They should be 1:1 ideally, and now people are averaging 25:1.”&lt;/p&gt;&lt;p&gt;A big contributor to the dramatic rise in levels of omega-6 fatt","PeriodicalId":9410,"journal":{"name":"Cancer Cytopathology","volume":"133 4","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cncy.70007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143762117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An integrated multi-omics biomarker approach using molecular profiling and microRNAs for evaluation of pancreatic cyst fluid","authors":"Mohamed H. Maher PharmD, PhD,&nbsp;Warapen Treekitkarnmongkol PhD,&nbsp;Sayak Ghatak MD,&nbsp;Jianliang Dai PhD,&nbsp;Suyu Liu PhD,&nbsp;Tristian Nguyen BSc,&nbsp;Dzifa Y. Duose PhD,&nbsp;Michael P. Kim MD,&nbsp;Tony Y. Hu PhD,&nbsp;Mark W. Hurd PhD,&nbsp;Pamela L. Paris PhD,&nbsp;Kimberly S. Kirkwood MD,&nbsp;Anirban Maitra MBBS,&nbsp;Rajyalakshmi Luthra PhD,&nbsp;Subrata Sen PhD,&nbsp;Sinchita Roy-Chowdhuri MD, PhD","doi":"10.1002/cncy.70008","DOIUrl":"10.1002/cncy.70008","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Classification and risk stratification of pancreatic cysts are challenging because of limited radiographic and cytomorphologic features. Although molecular profiling has emerged as an ancillary test for pancreatic cyst fluid (PCF), additional high-sensitivity and -specificity biomarkers are still needed for improved classification.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>In this study, PCF from 93 patients, including intraductal papillary mucinous neoplasms (<i>n</i> = 65), mucinous cystic neoplasms (<i>n</i> = 9), serous cystadenomas (<i>n</i> = 9), pancreatic cyst not otherwise specified (<i>n</i> = 8), and pseudocysts (<i>n</i> = 2), were evaluated for biomarkers. Molecular profiling by next-generation sequencing was performed, and a subset of the cases (<i>n</i> = 32) were interrogated with 2083 microRNAs (miRNAs) to evaluate their use for pancreatic cyst risk stratification.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>As independent PCF biomarkers in 32 cases with histologic diagnoses, three miRNAs performed significantly better than mutant <i>KRAS</i>, mutant <i>GNAS</i>, carcinoembryonic antigen (CEA), and serum carbohydrate antigen 19-9 (CA19-9) in discriminating high-risk from low-risk cysts. The three elevated miRNAs in combination with mutant <i>KRAS</i>, mutant <i>GNAS</i>, and serum CA19-9 displayed similar diagnostic performance (miR-4461: area under the curve [AUC], 0.950; 95% confidence interval [CI], 0.800–1; miR-6723-5p: AUC, 0.958; 95% CI, 0.850–1; miR-6755-3p: AUC, 0.942; 95% CI, 0.816–1) in discriminating high-risk from low-risk cysts, when compared to mutant <i>KRAS</i>, mutant <i>GNAS</i>, CEA, and serum CA19-9 (AUC, 0.950; 95% CI, 0.825–1). In the absence of CA19-9, the three-marker panel of <i>KRAS</i>, <i>GNAS</i>, and miRNAs showed marginally improved performance compared with <i>KRAS</i>, <i>GNAS</i>, and CEA, which highlights the potential utility of miRNAs as biomarkers in PCF analysis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>These findings demonstrate that a multiomics biomarker approach with elevated PCF miRNAs with mutant <i>KRAS</i>, mutant <i>GNAS</i>, and serum CA19-9 may help in better detecting high-risk cysts for early clinical intervention.</p>\u0000 </section>\u0000 </div>","PeriodicalId":9410,"journal":{"name":"Cancer Cytopathology","volume":"133 4","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extended HPV genotyping and dual stain for the triage of primary HPV screen-positive cases: Practical guidance for the cytopathology laboratory","authors":"Robert A. Goulart MD,&nbsp;Ritu Nayar MD,&nbsp;Thomas Lorey MD,&nbsp;Nancy Joste MD,&nbsp;Mark H. Stoler MD","doi":"10.1002/cncy.70006","DOIUrl":"https://doi.org/10.1002/cncy.70006","url":null,"abstract":"<p>Because of many factors, the landscape of cervical cancer prevention is again at a pivot point within the United States. Primary human papillomavirus (HPV) screening has been recommended as the preferred testing method by the American Cancer Society since 2020. Although primary HPV testing provides high negative predictive value in screening, women who screen positive for HPV need triage using methods that have an optimal balance between sensitivity for precancer and the number of colposcopies required for detection. The triage test ideally should maximize specificity while also reassuring patients who test negative, although it should be acknowledged that no screening or triage test can entirely exclude disease in a screen-positive patient. While cervical cytology (the Papanicolaou test) triage of primary HPV screen-positive patients is currently recommended by most screening strategies, additional triage tests, specifically extended HPV genotyping and combined p16/Ki-67 dual-stain immunocytochemistry, are now approved by the US Food and Drug Administration and incorporated into cervical cancer screening and management guidelines. Incorporating these triage methods into practice should be achieved by using appropriate validation/verification and implementation steps and, in the case of dual-stain immunocytochemistry, appropriate cytologist/cytopathologist training. The US Food and Drug Administration approval of vaginal self-collection in May 2024 is another significant advance for increasing access to screening. These samples can only be tested using primary HPV screening platforms, and guidance for management has been endorsed by the ASCCP's enduring guidelines process. This review discusses issues that warrant consideration before implementation and provides practical guidance for the incorporation of self-collected specimens and extended genotyping/dual-stain tests into the workflow of the cytopathology laboratory.</p>","PeriodicalId":9410,"journal":{"name":"Cancer Cytopathology","volume":"133 4","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143571216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hepatocellular carcinoma: Morphological spectrum and subtyping as per the World Health Organization classification on FNA biopsy with cell block samples","authors":"Bhawana Dhiman MD,&nbsp;Reetu Kundu MD,&nbsp;Suvradeep Mitra MD,&nbsp;Naveen Kalra MD,&nbsp;Madhumita Premkumar MD, DM,&nbsp;Ajay Kumar Duseja MD, DM,&nbsp;Radhika Srinivasan MD, PhD","doi":"10.1002/cncy.70009","DOIUrl":"https://doi.org/10.1002/cncy.70009","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Hepatocellular carcinoma (HCC) may be diagnosed and further subclassified in surgical specimen as per the recent World Health Organization (WHO) classification into several distinct subtypes with prognostic implications. The aim of this study was to apply this WHO classification on fine-needle aspiration biopsy (FNAB) samples of HCC and describe their features.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>This was a retrospective analysis of all ultrasound-guided FNAB of liver mass lesions in patients with suspected HCC (<i>n</i> = 164) over a 7-year period. Detailed morphological assessment of cytopathological features and grading was done and correlated with each other. HCC was subtyped further in cases with available cell blocks (<i>n</i> = 126).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>A total of 164 cases of HCC were evaluated on FNAB with age range of 18–88 years (mean, 60 years), and with 140 (85.4%) male and 24 (14.6%) female patients. Grading performed on 160 cases of HCC (after excluding fibrolamellar HCC) revealed 23 well differentiated, 127 moderately differentiated, and 10 poorly differentiated HCCs. Subtyping was feasible in 126 cases, of which 26 cases (20.6%) showed specific subtypes that were steatohepatitic (8), lymphocyte-rich (8), fibrolamellar (4), neutrophil-rich (3), macrotrabecular massive (2), and clear cell HCC (1) with remaining cases (100) being conventional HCC, no special type.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The study demonstrates the feasibility of subtyping HCC (as per the current WHO classification) for the first time on FNAB with cell blocks that carries implication for prognostication and emphasizes the importance of obtaining tissue diagnosis by FNAB with cell blocks.</p>\u0000 </section>\u0000 </div>","PeriodicalId":9410,"journal":{"name":"Cancer Cytopathology","volume":"133 4","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143554487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}