JACS Au最新文献_第3页

IF 8.5

JACS Au Pub Date : 2025-04-01 DOI: 10.1021/jacsau.5c0011410.1021/jacsau.5c00114

Xiaofeng Lu, Xiaoyu Du, Dong Zhong, Renjie Li, Junjie Cao, Shuo Huang* and Yuqin Wang*,

{"title":"Nanopore Environmental Analysis","authors":"Xiaofeng Lu, Xiaoyu Du, Dong Zhong, Renjie Li, Junjie Cao, Shuo Huang* and Yuqin Wang*, ","doi":"10.1021/jacsau.5c0011410.1021/jacsau.5c00114","DOIUrl":"https://doi.org/10.1021/jacsau.5c00114https://doi.org/10.1021/jacsau.5c00114","url":null,"abstract":"As global pollution continues to escalate, timely and accurate monitoring is essential for guiding pollution governance and safeguarding public health. The increasing diversity of pollutants across environmental matrices poses a significant challenge for instrumental analysis methods, which often require labor-intensive and time-consuming sample pretreatment. Nanopore technology, an emerging single-molecule technique, presents a promising solution by enabling the rapid identification of multiple targets within complex mixtures with minimal sample preparation. A wide range of pollutants have been characterized using natural biological nanopores or artificial solid-state nanopores, and their distinct advantages include simple sample preparation, high sensitivity, and rapid onsite analysis. In particular, long-read nanopore sequencing has led to dramatic improvements in the analyses of environmental microbial communities, allows species-level taxonomic assignment using amplicon sequencing, and simplifies the assembly of metagenomes. In this Perspective, we review the latest advancements in analyzing chemical and biological pollutants through nanopore sensing and sequencing techniques. We also explore the challenges that remain in this rapidly evolving field and provide an outlook on the potential for nanopore environmental analysis to transform pollution monitoring, risk assessment, and public health protection.","PeriodicalId":94060,"journal":{"name":"JACS Au","volume":"5 4","pages":"1570–1590 1570–1590"},"PeriodicalIF":8.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/jacsau.5c00114","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143878185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bivalent Surface Attachment via Cysteine Thiol Results in Efficient and Stereoselective Abiotic Peptide Synthesis 通过半胱氨酸硫醇的二价表面附着导致高效和立体选择性的非生物肽合成

IF 8.5

JACS Au Pub Date : 2025-03-31 DOI: 10.1021/jacsau.5c0015310.1021/jacsau.5c00153

Daniel P. Molland*, Isabella B. Rhyu, Jon Wade* and Jason R. Schnell*,

{"title":"Bivalent Surface Attachment via Cysteine Thiol Results in Efficient and Stereoselective Abiotic Peptide Synthesis","authors":"Daniel P. Molland*, Isabella B. Rhyu, Jon Wade* and Jason R. Schnell*, ","doi":"10.1021/jacsau.5c0015310.1021/jacsau.5c00153","DOIUrl":"https://doi.org/10.1021/jacsau.5c00153https://doi.org/10.1021/jacsau.5c00153","url":null,"abstract":"Surface-catalyzed peptide bond formation may have been an important source of peptides for abiogenesis, but model peptide synthesis reactions using the consensus set of 10 abiotic amino acids give only modest rates of peptide bond formation. Additionally, the peptides are typically limited in length to a small number of amino acids and stereoselective amino acid incorporation is weak or absent. An abiotic route for the high-yield synthesis of cysteine from serine was recently reported by Foden et al. (Science 2020, 370, 865–869), indicating that, in some environments, prebiotic cysteine may also have been available. Here, we show that the presence of cysteine dramatically increases the yields of surface-catalyzed peptide synthesis reactions in a hydrothermal vent solvent model containing achiral silicate minerals and that the reaction exhibits a strong stereoselective bias toward l-cysteine. Solid state NMR confirmed that cysteine associates bivalently with silicates at alkaline pH via both the carboxylate and the sulfur groups. Polarization-resolved IRRAS indicates that the bivalent adsorption stereospecifically orients the reactive amino group, providing a mechanism for stereoselective incorporation of l-cysteine. Stereoselective rates of peptide bond formation in surface-catalyzed peptide bond formation are expected to occur for any amino acid able to form sufficiently strong side chain–silicate interactions at alkaline pH. The high nucleophilicity of the thiol group produces unusually high reaction rates and stereoselectivity in such reactions, in addition to conferring transition metal ion binding to the peptide products. The potential benefits of reactive sulfur species for abiogenesis suggest that they may be useful biosignatures in the search for habitable extraterrestrial environments.","PeriodicalId":94060,"journal":{"name":"JACS Au","volume":"5 4","pages":"1922–1931 1922–1931"},"PeriodicalIF":8.5,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/jacsau.5c00153","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143878363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancing Nanomaterial-Based Strategies for Alzheimer’s Disease: A Perspective 推进基于纳米材料的阿尔茨海默病治疗策略：展望

IF 8.5

JACS Au Pub Date : 2025-03-31 DOI: 10.1021/jacsau.5c0000210.1021/jacsau.5c00002

Hong Zhou, Xiuhua Yin, Guanqiao Zhang, Zaixing Yang and Ruhong Zhou*,

{"title":"Advancing Nanomaterial-Based Strategies for Alzheimer’s Disease: A Perspective","authors":"Hong Zhou, Xiuhua Yin, Guanqiao Zhang, Zaixing Yang and Ruhong Zhou*, ","doi":"10.1021/jacsau.5c0000210.1021/jacsau.5c00002","DOIUrl":"https://doi.org/10.1021/jacsau.5c00002https://doi.org/10.1021/jacsau.5c00002","url":null,"abstract":"Alzheimer’s disease (AD) is a complex neurodegenerative disorder and the most common cause of dementia. By 2050, the number of AD cases is projected to exceed 131 million, placing significant strain on healthcare systems and economies worldwide. The pathogenesis of AD is multifactorial, involving hypotheses/mechanisms, such as amyloid-β (Aβ) plaques, tau protein hyperphosphorylation, cholinergic neuron damage, oxidative stress, and inflammation. Despite extensive research, the complexity of these potentially entangled mechanisms has hindered the development of treatments that can reverse disease progression. Nanotechnology, leveraging the unique physical, electrical, magnetic, and optical properties of nanomaterials, has emerged as a promising approach for AD treatment. In this Perspective, we first outlined the major current pathogenic hypotheses of AD and then reviewed recent advances in nanomaterials in addressing these hypotheses. We have also discussed the challenges in translating nanomaterials into clinical applications and proposed future directions, particularly the development of multifunctional and multitarget nanomaterials, to enhance their therapeutic efficacy and clinical applicability in AD treatment.","PeriodicalId":94060,"journal":{"name":"JACS Au","volume":"5 4","pages":"1519–1537 1519–1537"},"PeriodicalIF":8.5,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/jacsau.5c00002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143878362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Toward Hydrogen Mobility: Challenges and Strategies in Electrocatalyst Durability for Long-Term PEMFC Operation 迈向氢迁移：PEMFC长期运行电催化剂耐久性的挑战与策略

IF 8.5

JACS Au Pub Date : 2025-03-30 DOI: 10.1021/jacsau.5c0017310.1021/jacsau.5c00173

Subin Park, Eungjun Lee, Yoonsu Park, Myeong-Geun Kim* and Sung Jong Yoo*,

{"title":"Toward Hydrogen Mobility: Challenges and Strategies in Electrocatalyst Durability for Long-Term PEMFC Operation","authors":"Subin Park, Eungjun Lee, Yoonsu Park, Myeong-Geun Kim* and Sung Jong Yoo*, ","doi":"10.1021/jacsau.5c0017310.1021/jacsau.5c00173","DOIUrl":"https://doi.org/10.1021/jacsau.5c00173https://doi.org/10.1021/jacsau.5c00173","url":null,"abstract":"Proton exchange membrane fuel cells (PEMFCs) are emerging as a key technology in the transition to hydrogen-based energy systems, particularly for heavy-duty vehicles (HDVs) that face operational challenges, such as frequent startup-shutdown cycles and fuel starvation. However, the widespread adoption of PEMFCs has been limited by their durability and long-term performance issues, which are crucial for heavy-duty applications. This Perspective focuses on recent advancements in PEMFC catalysts and supports, with an emphasis on strategies to enhance their durability. We introduce Pt-based intermetallic catalysts, including Pt transition metal (TM) alloys, which offer improved stability and activity through regular atomic arrangements and strengthened metal–support interactions. Hybrid catalysts combining Pt with M-N-C (M = Fe, Co) have shown promise in boosting performance by enhancing the catalytic activity while reducing the platinum content. Moreover, stringent conditions must be met to meet the HDV requirements. Consequently, alternative support materials, such as metal oxides and graphitized carbons, have been introduced to enhance both the corrosion resistance and the electrical conductivity, thereby addressing the limitations of conventional carbon supports. Structural innovations and material advancements are essential for optimizing catalysts and supports to achieve long-term PEMFC performance. This Perspective provides a comprehensive overview of key developments in catalyst and support design, offering insights into current challenges and future directions for achieving durable and cost-effective PEMFCs.","PeriodicalId":94060,"journal":{"name":"JACS Au","volume":"5 4","pages":"1617–1632 1617–1632"},"PeriodicalIF":8.5,"publicationDate":"2025-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/jacsau.5c00173","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143878361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbially Derived P═S and P═Se Bond Formation 微生物来源的P = S和P = Se键形成

IF 8.5

JACS Au Pub Date : 2025-03-29 DOI: 10.1021/jacsau.5c0026210.1021/jacsau.5c00262

Connor L. Trotter, Yuta Era, Rory Gordon, Samantha Law, Christopher H. Switzer and Stephen Wallace*,

引用次数: 0

Selective Native N(in)–H Bond Activation in Peptides with Metallaphotocatalysis 金属光催化多肽中选择性天然N(in) -H键激活

IF 8.5

JACS Au Pub Date : 2025-03-28 DOI: 10.1021/jacsau.5c0011910.1021/jacsau.5c00119

José A. C. Delgado*, Jéssica C. Amaral, Paula S. Penteado, Antonio G. Ferreira, Maria Fátima G. F. da Silva, Burkhard König* and Márcio W. Paixão*,

{"title":"Selective Native N(in)–H Bond Activation in Peptides with Metallaphotocatalysis","authors":"José A. C. Delgado*, Jéssica C. Amaral, Paula S. Penteado, Antonio G. Ferreira, Maria Fátima G. F. da Silva, Burkhard König* and Márcio W. Paixão*, ","doi":"10.1021/jacsau.5c0011910.1021/jacsau.5c00119","DOIUrl":"https://doi.org/10.1021/jacsau.5c00119https://doi.org/10.1021/jacsau.5c00119","url":null,"abstract":"The development of chemical methods enabling site-selective incorporation of noncanonical amino acids into peptide backbones with precise functional tailoring remains a critical challenge. Particularly compelling is the use of underexplored endogenous amino acid hotspots, such as the N(in) of tryptophan, as versatile anchors for diversification. Herein, we report a chemoselective N(sp2)–H bond activation strategy targeting native tryptophan residues within peptide frameworks, exemplified by GLP-1 (7–37), using nickel metallaphotocatalysis under postsynthetic solid-phase conditions. This selective N(in)-arylation reaction proceeds efficiently within 3 h of light irradiation in highly functionalized heterogeneous environments, employing minimal excesses of electrophile and base, alongside catalytic quantities of nickel, ligand, and photocatalyst. The method affords homogeneous peptide products with high chemoselectivity and operational simplicity. We envision that this strategy could contribute to advancing the design of the next-generation long-acting class II G protein-coupled receptor agonist therapeutics.","PeriodicalId":94060,"journal":{"name":"JACS Au","volume":"5 4","pages":"2040–2046 2040–2046"},"PeriodicalIF":8.5,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/jacsau.5c00119","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143878319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancing the Understanding of Oxygen Vacancies in Ceria: Insights into Their Formation, Behavior, and Catalytic Roles 推进对氧化铈中氧空位的理解：对其形成、行为和催化作用的洞察

IF 8.5

JACS Au Pub Date : 2025-03-28 DOI: 10.1021/jacsau.5c0009510.1021/jacsau.5c00095

Zhong-Kang Han, Wen Liu and Yi Gao*,

{"title":"Advancing the Understanding of Oxygen Vacancies in Ceria: Insights into Their Formation, Behavior, and Catalytic Roles","authors":"Zhong-Kang Han, Wen Liu and Yi Gao*, ","doi":"10.1021/jacsau.5c0009510.1021/jacsau.5c00095","DOIUrl":"https://doi.org/10.1021/jacsau.5c00095https://doi.org/10.1021/jacsau.5c00095","url":null,"abstract":"Oxygen vacancies (OV’s) in ceria (CeO2) are critical structural and electronic features that underpin ceria’s remarkable oxygen storage capacity, redox catalytic performance, and wide-ranging applications in catalysis, solid oxide fuel cells, and gas sensors. These vacancies, which result from the removal of oxygen atoms, enable dynamic oxygen exchange between the solid and its environment, profoundly influencing ceria’s catalytic properties. The intricate surface structures of ceria play a key role in determining its properties and its interactions with supported metal catalysts. Over the past decade, advancements in state-of-the-art in situ characterizations, first-principles calculations, and emerging machine learning frameworks have significantly enhanced our understanding of the formation mechanisms, behaviors, and catalytic roles of OV’s. This perspective highlights recent experimental and theoretical progress in ceria surface research, emphasizing the dynamic interplay between surface structures and reactive environments. Additionally, the perspective addresses key challenges in elucidating ceria’s defect chemistry and explores opportunities to tailor its properties using multiscale modeling and AI-driven methodologies.","PeriodicalId":94060,"journal":{"name":"JACS Au","volume":"5 4","pages":"1549–1569 1549–1569"},"PeriodicalIF":8.5,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/jacsau.5c00095","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143878320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isothermal Disorder-to-Order Transitions of DNA Origami Structures Induced by Alternative Component Subsets 由可选组分亚群诱导的DNA折纸结构的等温无序到有序转变

IF 8.5

JACS Au Pub Date : 2025-03-27 DOI: 10.1021/jacsau.5c0019510.1021/jacsau.5c00195

Yue Wang, Biancheng Wei, Qinglin Xia, Lei Ren, Bin Li, Linjie Guo, Ying Zhu, Lihua Wang*, Kai Jiao* and Jiang Li*,

{"title":"Isothermal Disorder-to-Order Transitions of DNA Origami Structures Induced by Alternative Component Subsets","authors":"Yue Wang, Biancheng Wei, Qinglin Xia, Lei Ren, Bin Li, Linjie Guo, Ying Zhu, Lihua Wang*, Kai Jiao* and Jiang Li*, ","doi":"10.1021/jacsau.5c0019510.1021/jacsau.5c00195","DOIUrl":"https://doi.org/10.1021/jacsau.5c00195https://doi.org/10.1021/jacsau.5c00195","url":null,"abstract":"DNA origami technology has shown potential across various applications, including the construction of molecular machines. Among these, mimicking the complex structural transitions of natural biomolecules in physiological environments remains a long-standing pursuit. Here, inspired by intrinsically disordered proteins, we propose a strategy for inducing disorder-to-order transitions in DNA origami structures at room temperature using alternative component subsets. In a triangular DNA origami model, we define three subsets of its constitutional DNA staples based on their spatial distributions along the scaffold. Atomic force microscopy and molecular dynamics simulations show that the individual subsets result in metastable assemblies with disordered morphologies and elevated free-energy fluctuations compared with those generated by the complete set of staples. Notably, after the addition of the remaining staples, the irregular structures transform into ordered triangular architectures within 2 h at room temperature, achieving yields of up to ∼60%. These findings suggest that these controlled folding pathways in DNA origami can robustly converge on the global energy minimum at room temperature, thereby providing a promising alternative strategy for engineering biomimetic DNA molecular machines.","PeriodicalId":94060,"journal":{"name":"JACS Au","volume":"5 4","pages":"1641–1648 1641–1648"},"PeriodicalIF":8.5,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/jacsau.5c00195","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143878318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ammonium-Based Plastic Crystals as Solid-State Electrolytes for Lithium and Sodium Batteries 作为锂和钠电池固态电解质的氨基塑料晶体

IF 8.5

JACS Au Pub Date : 2025-03-26 DOI: 10.1021/jacsau.4c0108610.1021/jacsau.4c01086

Manuel Salado, Thomas H. Smith, Nanditha Sirigiri, Fangfang Chen, Luke A. O’Dell, Jennifer M. Pringle and Maria Forsyth*,

{"title":"Ammonium-Based Plastic Crystals as Solid-State Electrolytes for Lithium and Sodium Batteries","authors":"Manuel Salado, Thomas H. Smith, Nanditha Sirigiri, Fangfang Chen, Luke A. O’Dell, Jennifer M. Pringle and Maria Forsyth*, ","doi":"10.1021/jacsau.4c0108610.1021/jacsau.4c01086","DOIUrl":"https://doi.org/10.1021/jacsau.4c01086https://doi.org/10.1021/jacsau.4c01086","url":null,"abstract":"Organic ionic plastic crystals (OIPCs) are a promising class of solid materials composed of organic cations and inorganic anions, increasingly explored for use as solid-state electrolytes (SSEs). These materials offer a safer alternative to conventional carbonate-based electrolytes in lithium and sodium ion batteries. In this study, lithium and sodium salts were incorporated into tetramethylammonium bis(fluorosulfonyl)imide ([N1111][FSI]), yielding solid state electrolytes with notable properties, including high ionic conductivities (1.79 mS·cm–1 for LiFSI doped and 3.2 mS·cm–1 NaFSI doped, both at 80 °C), elevated diffusion coefficients (up to 3.83 × 10–11 m2·s–1 for Li+ at 80 °C), and high transference numbers (0.8 and 0.4, for Li and Na, respectively). To date, except for ceramic and glassy ion conductors, there has been no significant research demonstrating true solid-state behavior with Li+ or Na+ ion transport fully decoupled from the motion of the host structure. Furthermore, these electrolytes have exhibited impressive current densities up to 3.5 mA·cm–2 during Li|Li and 2.9 mA·cm–2 for Na|Na symmetric cell cycling at room temperature. As a result, these materials hold considerable potential for enhancing both Li and Na electrochemical energy storage technologies, combining both improved efficiency and safety features.","PeriodicalId":94060,"journal":{"name":"JACS Au","volume":"5 4","pages":"1663–1676 1663–1676"},"PeriodicalIF":8.5,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/jacsau.4c01086","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143878317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sorting Extrusion-Prepared Coreless Cell Membrane Vesicles of Mixed Orientations by Functional DNA Probes 用功能DNA探针分选挤压制备的混合取向无芯细胞膜囊泡

IF 8.5

JACS Au Pub Date : 2025-03-25 DOI: 10.1021/jacsau.4c0125110.1021/jacsau.4c01251

Jing Ye, Fan Xu, Zhihao Xu, Caiqing Yuan, Pengfei Hou, Dunkai Wu, Weifeng Han, Shufan Pan, Li Pan, Donglei Yang* and Pengfei Wang*,

{"title":"Sorting Extrusion-Prepared Coreless Cell Membrane Vesicles of Mixed Orientations by Functional DNA Probes","authors":"Jing Ye, Fan Xu, Zhihao Xu, Caiqing Yuan, Pengfei Hou, Dunkai Wu, Weifeng Han, Shufan Pan, Li Pan, Donglei Yang* and Pengfei Wang*, ","doi":"10.1021/jacsau.4c0125110.1021/jacsau.4c01251","DOIUrl":"https://doi.org/10.1021/jacsau.4c01251https://doi.org/10.1021/jacsau.4c01251","url":null,"abstract":"Cell membrane vesicles (CMVs) have been extensively used as delivery vehicles for a variety of cargos, which are generally prepared via membrane extrusion. Extruded CMVs are not necessarily to have the outer membrane facing outward due to the randomness of membrane wrapping. Nanoparticles have been used to serve as cores to direct the membrane orientation of CMVs; nevertheless, there is a lack of methods to efficiently sort coreless CMVs of desired orientations. Herein, we utilized a group of functional DNA probes to reveal the random distribution of membrane orientations of coreless CMVs after extrusion, producing either right-side-out vesicles (RSVs) or inside-out vesicles (ISVs). More importantly, DNA probes that protrude out from the outer membrane can serve as handles for efficiently sorting out RSVs from ISVs to produce vesicles with a dominant right-side-out orientation. We investigated three methods to enrich RSVs, including strand displacement reaction, photo cleavage (PC), and enzymatic cleavage. Among them, PC exhibits the highest enrichment efficiency (∼93%) and RSVs purity (∼85.4%), which therefore is recommended for future applications. This work revealed the mixed orientations of coreless CMVs and provided a technical platform to efficiently enrich CMVs of wanted membrane orientations that shall be useful toward a vast array of biomedical applications.","PeriodicalId":94060,"journal":{"name":"JACS Au","volume":"5 4","pages":"1728–1737 1728–1737"},"PeriodicalIF":8.5,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/jacsau.4c01251","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143878314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0