International journal of radiation biology最新文献

{"title":"Historical insights from past SMRs used in nuclear propulsion and electricity generation in remote locations.","authors":"Trevor J Stocki","doi":"10.1080/09553002.2026.2629246","DOIUrl":"https://doi.org/10.1080/09553002.2026.2629246","url":null,"abstract":"<p><strong>Purpose and introduction: </strong>A number of countries, including Canada, are researching the development of Small Modular nuclear Reactors (SMR) for electrical power generation. This paper aims to point impact assessors to other previous historical deployments of SMRs. These types of reactors could be used to power remote locations, for heavy industry applications, and for on-grid applications. One possible use for a SMR is to provide power to remote Arctic communities. Small nuclear reactors are not a new concept. The Americans constructed small nuclear reactors for remote areas in the 1960s, under the U.S. Army Nuclear Power Program, where they built and operated four reactors in remote regions of Alaska, Greenland, Antarctica, and in the Panama Canal Zone on a barge. More recently, Russia placed two nuclear reactors on a barge and towed it to a remote Arctic community to replace diesel power generation. It is now providing electricity to that remote location. Health Canada, a federal department of the Government of Canada, plays a role in human health risk assessment for major resource and infrastructure projects in Canada and is therefore interested in the history of SMRs and the potential radio-ecological consequences of future projects in terms of radiation dose received by humans. Because Health Canada also has a role in emergency preparedness, this paper also examines the historical issues around the AVR reactor, a high temperature gas cooled reactor, which is a SMR reactor type being considered in Canada.</p><p><strong>Conclusions: </strong>This review paper provides a brief overview of SMRs in general and discusses potential challenges for modeling human health risk in remote locations. For example, in doing assessments for nuclear reactors, often an environmental transport model is used which is dependent on transfer factors which are determined from measurements. These transfer factors most likely will change in the Arctic environment.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-12"},"PeriodicalIF":2.4,"publicationDate":"2026-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146215207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanism, measurement and significance of oxidative DNA damage<i>- a tribute to Miral Dizdaroglu</i>.","authors":"Barry Halliwell","doi":"10.1080/09553002.2026.2620569","DOIUrl":"https://doi.org/10.1080/09553002.2026.2620569","url":null,"abstract":"<p><strong>Purpose: </strong>To explain the multiple high level scientific contributions of Miral Dizdaroglu to our understanding of oxidatively generated DNA damage, which plays a pivotal role in radiation chemistry and multiple human diseases, especially cancer.</p><p><strong>Materials and methods: </strong>A literature review of Miral's contributions and his interactions with my research group.</p><p><strong>Results: </strong>Miral was a pioneer in understanding the mechanisms, measurement and repair of damage to DNA (and, more recently, RNA) by free radicals and other reactive oxygen species (ROS), initially in radiation systems and later in biological systems, including the human body. My research group learned an enormous amount from him when I was privileged to work together with him and publish multiple joint papers. In this review, I describe the history of the field from my perspective and describe several of Miral's fundamental contributions, as well as some of the controversies surrounding his work.</p><p><strong>Conclusion: </strong>Miral's research contributions were outstanding, and we have lost a major contributor to the field of oxidatively generated damage to nucleic acids.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-9"},"PeriodicalIF":2.4,"publicationDate":"2026-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146215193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular classification of radiation-induced meningiomas.","authors":"Yosef Ellenbogen, Vikas Patil, Alexander P Landry, Leeor S Yefet, Justin Z Wang, Andrew Ajisebutu, Chloe Gui, Jeff Liu, Phooja Persaud, Kenneth D Aldape, Farshad Nassiri, Gelareh Zadeh","doi":"10.1080/09553002.2026.2618533","DOIUrl":"https://doi.org/10.1080/09553002.2026.2618533","url":null,"abstract":"<p><strong>Purpose: </strong>Radiation-induced meningiomas (RIMs) are an uncommon late complication of cranial irradiation that frequently display aggressive behavior. Although recent genomic and epigenomic studies have redefined sporadic meningiomas into four molecular groups with distinct biological and clinical characteristics, the same analysis has not yet been conducted on RIMs. This study sought to contextualize RIMs within the current methylation-based meningioma classification.</p><p><strong>Methods: </strong>DNA methylation data from RIMs (<i>n</i> = 20) were integrated with a reference cohort of sporadic (<i>n</i> = 121) meningiomas previously used to define molecular subgroups. Molecular group membership was assigned using a supervised machine-learning approach. Copy-number alterations and pathway enrichment analyses were derived from methylation data, and clinical features were compared between RIMs and sporadic meningiomas.</p><p><strong>Results: </strong>Supervised molecular classification assigned 70% RIMs to the hypermetabolic subtype. The RIM cohort demonstrated broad DNA hypomethylation enriched for metabolic and biosynthetic pathways. Copy-number profiling revealed widespread chromosomal instability, including recurrent 22q loss involving NF2 and SMARCB1 as well as PTEN, MYB, and C19MC, consistent with the copy number alterations observed in hypermetabolic meningiomas.</p><p><strong>Conclusions: </strong>RIMs predominantly align with the hypermetabolic molecular group, characterized by metabolic pathway activation and genomic instability. This distribution indicates a distinct molecular profile compared with sporadic meningiomas.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-7"},"PeriodicalIF":2.4,"publicationDate":"2026-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146109154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The multifaceted behavior of electron attachment in 8-bromo-purine derivatives and the connection with DNA damage^†.","authors":"Chryssostomos Chatgilialoglu","doi":"10.1080/09553002.2026.2617595","DOIUrl":"https://doi.org/10.1080/09553002.2026.2617595","url":null,"abstract":"<p><strong>Purpose: </strong>This review summarizes the discoveries of 8-bromopurine nucleosides (8-Br-Pu), particularly 8-bromo-2'-deoxyadenosine (8-Br-dA) and 8-bromo-2'-deoxyguanosine (8-Br-dG), in chemistry and biology over the past two decades. It compiles available data on the reactions of hydrated electrons (e_aq^-) with various 8-bromopurines, as determined by pulse radiolysis and supported by theoretical studies. Three distinct mechanistic pathways are identified: dissociative electron attachment, sequential electron transfer-proton transfer, and concerted electron-proton transfer. This review also highlights the use of 8-Br-dA and 8-Br-dG in the synthesis of a library of 5',8-cyclopurine nucleosides (cPu) for quantifying them in genetic material and incorporating them into oligonucleotides (ODNs) for DNA repair research. Additionally, the summary covers the use of 8-Br-dA and 8-Br-dG embedded in various ODNs to study excess electron transfer (EET), their potential as radiosensitizers, and their formation in vivo via hypobromous acid.</p><p><strong>Conclusion: </strong>Based on radiation chemistry, our understanding of the one-electron reduction of 8-Br-dA and 8-Br-dG has been enhanced substantially. This mechanistic background is crucial for a better understanding of and addressing their significant roles in the biological environment, such as DNA radiosensitizers for cancer radiation therapy or as biomarkers for early inflammation.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-14"},"PeriodicalIF":2.4,"publicationDate":"2026-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146088417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Radiobiological consequences evaluation following a hypothetical nuclear event based on the radionuclide-to-inventory release ratio.","authors":"Hugo Menossi, Isabela Alves, Jonas Antonio Ederli, Maíra Uliana, Rodrigo Curzio, Andre Tavares, Edson Andrade","doi":"10.1080/09553002.2025.2608747","DOIUrl":"10.1080/09553002.2025.2608747","url":null,"abstract":"<p><strong>Purpose: </strong>This study assesses the radiological impact of radionuclide dispersion for the first four days after a hypothetical nuclear accident scenario, utilizing the HotSpot Health Physics software developed by the Lawrence Livermore National Laboratory (LLNL).</p><p><strong>Materials and methods: </strong>A Gaussian plume model was used to estimate the dispersion of I-131 and Cs-137 under various atmospheric conditions.</p><p><strong>Results: </strong>The study's key findings include an analysis of the Total Effective Dose Equivalent (TEDE) along a simulated contamination plume, highlighting the impact of atmospheric stability on radiation levels. It also assesses the radiobiological risks, noting that young people and women are especially vulnerable, as the risk of thyroid or leukemia may vary by a factor of 10⁴ depending on location and atmospheric stability conditions. The analysis also indicated that the TEDE generated by the inventory release could range from 0.1 to 10 Sv of whole-body exposure within the first 10 km downwind under stable atmospheric conditions.</p><p><strong>Conclusions: </strong>Even minor fluctuations in TEDE caused by local atmospheric stability can significantly affect the estimated risks of thyroid cancer and leukemia. This may alter the logistical resources required and the epidemiological methods used to identify and prioritize concerns. Implementing environmental monitoring and strategies such as distributing stable iodine and enforcing dietary restrictions during the initial phases of nuclear emergencies may be guided by risk assessments based on simulations.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"290-299"},"PeriodicalIF":2.4,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145907431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of gamma radiation seed priming on glucosinolate metabolism, microbial dynamics, and antimicrobial activity in Brassicaceae sprouts.","authors":"Sultan Fahad Alhujaili, Feras Alafer, Abdulaziz Saad A Alshabibi, Emad A Alsherif, Shereen Magdy Korany, Abeer S Aloufi, Yousef Alhaj Hamoud, Hiba Shaghaleh, Nadia H Mohamed, Samy Selim","doi":"10.1080/09553002.2025.2587577","DOIUrl":"10.1080/09553002.2025.2587577","url":null,"abstract":"<p><strong>Purpose: </strong>Sprouting boosts bioactive compounds in Brassicaceae, including glucosinolates, amino acids, and anthocyanins. This study examined gamma radiation seed priming effects on growth, metabolism, and bioactive accumulation in <i>Nasturtium officinale, Eruca sativa</i>, and <i>Raphanus raphanistrum</i> sprouts.</p><p><strong>Materials and methods: </strong>Seeds of the three species were primed with gamma radiation (0.1 kGy/h) and grown under controlled conditions. Biomass was measured; metabolic profiling quantified glucosinolates, amino acids, flavonoids, and anthocyanins. Enzymatic activities for glucosinolate and flavonoid biosynthesis were assessed. Antioxidant potential was assessed using FRAP and DPPH assays; antimicrobial activity was tested against Escherichia coli and Enterococcus faecalis. Microbial counts (APCs and Coliforms) were measured.</p><p><strong>Results: </strong>Gamma radiation increased biomass by 48%, 36%, and 71% in <i>N. officinale, E. sativa</i>, and <i>R. raphanistrum</i>, respectively. Glucosinolates rose, especially glucoerucin (up to 227% in E. sativa) and glucoraphenin (up to 60%), linked to higher precursor amino acids (leucine, tryptophan, methionine), glutathione, and activities of glutathione S-transferase and sulfotransferase. Myrosinase activity increased, boosting sulforaphane. Flavonoids surged: quercetin (48 -191%), kaempferol (75 -172%), anthocyanins (42 -60%), with elevated PAL, CHS, 4CL, and C4H activities. Antioxidant and antimicrobial (40 -77%) potentials improved, though APCs and coliforms rose.</p><p><strong>Conclusions: </strong>Gamma radiation priming enhances growth, secondary metabolite accumulation, antioxidant potential, and antimicrobial activity in Brassicaceae sprouts. It offers a promising method to improve the nutritional and functional qualities of edible sprouts, aiding food safety and health.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"61-74"},"PeriodicalIF":2.4,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145552435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low-dose ionizing radiation augmenting the reproductive fitness of radio-sterilized moths, <i>Spodoptera litura</i> (Fabr.): an approach toward increasing the efficiency of 'Inherited Sterility technique' for Lepidopteran pest control.","authors":"Rakesh Kumar Seth, Neha Vimal, Nilza Angmo, Madhumita Sengupta, Ranjana Seth","doi":"10.1080/09553002.2025.2588404","DOIUrl":"10.1080/09553002.2025.2588404","url":null,"abstract":"<p><strong>Purpose: </strong>Low-dose ionizing radiation (LDIR) was reconnoitered to improve the reproductive fitness of radio-sterilized moths, <i>Spodoptera litura</i> (Fabr.) to be used in exercising radiation mediated 'Inherited sterility (F1) technique (IS) for this Lepidopteran pest suppression.</p><p><strong>Materials and methods: </strong>Various reproductive features were evaluated of the partially sterilized male moths (irradiated at 130 Gy), primed with potential hormetic doses (0.75-1 Gy as LDIR) in their ontogeny, in relation to response of unprimed sterilized moths. The expression of genes related to sperm dynamics and viability was ascertained in primed radio-sterilized moths. Mating competitiveness of 1 Gy (egg) primed radio-sterilized male moths was assessed in field simulated cages.</p><p><strong>Results: </strong>Radio-sterilized male moths, having prior hormetic exposure in various ontogenic stages, viz., 0.75 Gy (egg), 1 Gy (egg/larva/pupa), showed longer lifespan, increased mating success, and enhanced sperm dynamics in comparison to unprimed radio-sterilized male moths. The expression of genes related to sperm dynamics was affected in sterilized moths but low dose priming improved their expression in sterilized moths. The expression of viability genes-<i>foxo</i> and <i>Sirtuin2like</i> was down regulated unlike the up-regulated expression of <i>atm</i>, <i>sod</i>, <i>cat</i>, and <i>p53</i> in radio-sterilized male moths in comparison to control, whereas priming influenced the expression of these genes in the sterilized moths. A higher mating competitiveness value (CV) was observed in LDIR primed radio-sterilized moths as compared to unprimed sterilized males.</p><p><strong>Conclusion: </strong>These findings indicate that radiation hormesis might be employed as promising mode to enhance the reproductive viability of the radio-sterilized male moths to be used in this nuclear tactic.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"153-168"},"PeriodicalIF":2.4,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145590540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential roles of DNA double strand break repair pathways in response to X-ray, proton, and alpha-particle irradiation.","authors":"Gerarda van de Kamp, Tim Heemskerk, Marta Rovituso, Roland Kanaar, Jeroen Essers","doi":"10.1080/09553002.2025.2590742","DOIUrl":"10.1080/09553002.2025.2590742","url":null,"abstract":"<p><strong>Purpose: </strong>High linear energy transfer (LET) radiation is more harmful than low LET radiation because it deposits energy in a concentrated manner, resulting in clustered DNA damage (CDD). Double strand breaks (DSBs) are among the most damaging types of DNA damage, and if not repaired, they may trigger cell death. DSBs can be repaired through three mechanisms: non-homologous end joining (NHEJ), homologous recombination (HR), and theta-mediated end joining (TMEJ). This study aimed to assess how these pathways contribute to repairing DSBs induced by low LET X-ray and proton radiation, and high LET alpha-particle radiation.</p><p><strong>Materials and methods: </strong>We used mouse embryonic stem (mES) cells lacking key repair proteins to examine clonogenic survival and the formation and resolution of 53BP1 foci, a DNA damage marker, after exposure to X-ray, proton, and alpha-particle radiation.</p><p><strong>Results and conclusions: </strong>The results showed increased sensitivity to X-ray and proton radiation in NHEJ, HR, and TMEJ repair-deficient cell lines compared to wild-type cells, with similar trends for both radiation types. Notably, Rad54-deficient cells showed slower resolution of 53BP1 foci after proton exposure, indicating increased reliance on HR for repairing proton-induced DSBs. Clonogenic survival assays revealed a relative biological effectiveness (RBE) of 4.6-5.8 for alpha-particles compared to protons and X-rays, confirming that alpha-particles are more effective at causing cell death. Our findings suggest that TMEJ is important for repairing DSBs caused by alpha-particles. This study highlights differences in repairing low LET versus high LET DNA damage, offering new insights for radiation biology and therapeutic strategies.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"105-115"},"PeriodicalIF":2.4,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145673083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Radiation‑induced impacts on female fertility and fecundity: perspectives from the viewpoint of radiation protection.","authors":"Hisanori Fukunaga, Nobuyuki Hamada","doi":"10.1080/09553002.2025.2606997","DOIUrl":"10.1080/09553002.2025.2606997","url":null,"abstract":"<p><strong>Purpose: </strong>This mini-review aims to synthesize current knowledge regarding radiation-induced effects on female fertility and reproductive capacity, and to identify knowledge gaps relevant to radiation protection.</p><p><strong>Methods: </strong>We critically examined evidence from developmental biology, animal models, clinical studies, and epidemiological investigations. Key topics include germ cell and follicle biology, radiation dose thresholds, therapeutic exposures, and environmental and occupational contexts.</p><p><strong>Results: </strong>Female germ cells are highly radiosensitive, with animal models indicating a mean lethal dose as low as 0.05-0.15 Gy in primordial oocytes. In humans, pelvic irradiation increases risks of acute ovarian failure, premature ovarian insufficiency, and infertility, depending on dose and age at exposure. Cranial irradiation further impairs fertility through damage to the hypothalamic-pituitary axis. In contrast, evidence from occupational and environmental exposures is scarce, with most studies addressing miscarriage or perinatal outcomes rather than infertility itself.</p><p><strong>Conclusions: </strong>While advances in oncofertility research have clarified therapeutic risks, systematic data on environmental and occupational exposures are lacking. Strengthened prospective studies and integration of fertility-specific biomarkers are urgently needed to inform comprehensive radiation protection strategies.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"216-220"},"PeriodicalIF":2.4,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145907412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The evaluation of the protective effect of selenium-L-methionine on testicular tissue damage induced by radiation.","authors":"Kudret Ensarioğlu Baktır, Oguz Galip Yıldız, Numan Baydilli, Eda Koseoglu, Kadir Yaray, Merve Civan, Arzu Hanım Yay","doi":"10.1080/09553002.2025.2607005","DOIUrl":"10.1080/09553002.2025.2607005","url":null,"abstract":"<p><strong>Background: </strong>Radiation induces pronounced and widespread histopathological damage in the testes, which exhibit a high degree of radiosensitivity; consequently, the utilization of effective radioprotective agents has become increasingly crucial for mitigating radiation-associated toxic outcomes, particularly infertility.</p><p><strong>Purpose: </strong>The present investigation aimed to comprehensively evaluate the capacity of selenium-L-methionine to mitigate radiation-induced histopathological and molecular alterations within testicular tissue, thereby assessing its potential as a radioprotective agent.</p><p><strong>Material and methods: </strong>Rats were randomized into four groups: Group 1 (control), Group 2 (rad group), which received a single 10 Gy irradiation on day 2; Group 3 (sel group), which received intraperitoneal selenium-L-methionine (4 mg/kg) for six consecutive days; and Group 4 (rad+sel group), which received the same selenium-L-methionine regimen followed by 10 Gy irradiation 30 minutes after the second day's administration. On the seventh day, all animals were euthanized, and testicular tissue and blood samples were collected for biochemical and histopathological analyses.</p><p><strong>Results: </strong>In the testicular tissues of the radiation-exposed groups, deformed and abnormal seminiferous tubule structures, a reduction in germ cell numbers, and sloughing of tubular epithelial cells were observed. Seminiferous tubule diameters, Johnsen's testicular biopsy scores, epididymal sperm motility, and the expression levels of Connexin 43, HSP70, PCNA, StAR, CAT, and SOD were decreased in the irradiated group, whereas TGFB1, IL-6, and MMP9 levels were increased. Selenium-L-methionine treatment largely reversed these radiation-induced changes.</p><p><strong>Conclusions: </strong>The addition of selenium-L-methionine to radiotherapy yielded promising radioprotective outcomes, and this therapeutic effect positions selenium-L-methionine as a potential novel radioprotective agent. Furthermore, the immunohistochemical markers used in the study-including MMP9, Connexin 43, HSP70, PCNA, and StAR served as sensitive indicators for detecting radiation-induced damage in testicular tissue. Nevertheless, larger-scale and long-term studies are required to validate these findings and to further substantiate the potential use of selenium-L-methionine as a radioprotective agent in clinical practice.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"255-265"},"PeriodicalIF":2.4,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145968186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}