Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Association最新文献

{"title":"The Effects of matrix metalloproteinase-14 Inhibition on diabetic Wound Healing.","authors":"Gui-Feng Zeng, Guotian Chen, Zhen Zhou, Hui-Guang Chen, Guo-Ping Zhang, Shui-Lan Zou, Ziyan Yuan, De-Hong Pan, Hai-Yang Zhou, Gui-Qing Wang, Da-Wei Zhang, Xiao-Dan Xia","doi":"10.1055/a-2943-8604","DOIUrl":"10.1055/a-2943-8604","url":null,"abstract":"<p><strong>Abstract: </strong>Matrix metalloproteinases play critical roles in wound repair, yet their context-dependent functions in diabetic wound healing remain elusive. In this study, we found that both the expression and activity of matrix metalloproteinase-14 were significantly elevated in diabetic foot ulcers. Using animal models, we further revealed the dual roles of matrix metalloproteinase-14 in wound healing. In normal rats, inhibition of matrix metalloproteinase-14 delayed wound closure, confirming its supportive role in physiological tissue repair. In contrast, in diabetic rats, pathologically increased matrix metalloproteinase-14 levels impaired healing, whereas moderate inhibition significantly accelerated wound repair. Among the inhibitors tested, the selective matrix metalloproteinase-14 inhibitor NSC405020 produced the most favorable therapeutic effect in diabetic wounds, markedly enhancing healing rates and collagen deposition at a concentration of 1 mg/mL. Compared with the broad-spectrum matrix metalloproteinase inhibitor ND336, NSC405020 demonstrated therapeutic safety and comparable efficacy without notable toxicity in a rat model. Collectively, these findings elucidate the context-dependent role of matrix metalloproteinase-14 in wound healing, suggesting that matrix metalloproteinase-14 may be a promising therapeutic target for diabetic wound healing. Therefore, our study provides a theoretical foundation for the development of effective and safe treatments for diabetic wound management.</p>","PeriodicalId":94001,"journal":{"name":"Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Association","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148803603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impaired Epidermal Renewal and Dermal Collagen Disorganization in a Dehydroepiandrosterone-Induced Mouse Model of Polycystic Ovary Syndrome.","authors":"Özlem Delibaş, Berna Özdenoğlu Kutlu, Serpil Ünver Saraydın","doi":"10.1055/a-2942-6488","DOIUrl":"https://doi.org/10.1055/a-2942-6488","url":null,"abstract":"<p><strong>Abstract: </strong>BACKGROUND: Polycystic ovary syndrome is a complex endocrine-metabolic disorder associated with various dermatological manifestations. However, the histomorphological alterations underlying these skin findings remain poorly characterized. This study aimed to investigate skin alterations associated with polycystic ovary syndrome in an experimental mouse model using histochemical staining, morphometric evaluation, and immunofluorescence analysis.</p><p><strong>Abstract: </strong>METHODS: An experimental study was performed using 16 Swiss albino female mice. The polycystic ovary syndrome model was induced by dehydroepiandrosterone (6 mg/100 g body weight; subcutaneous). Model validation was performed using ovarian histology, estrous cycle monitoring, body weight, fasting blood glucose, and the intraperitoneal glucose tolerance test. Skin tissues were evaluated using haematoxylin and eosin staining for epidermal and dermal thicknesses, Masson's trichrome staining for collagen content (quantified with ImageJ), and immunofluorescence staining for Ki-67 and vascular endothelial growth factor immunoreactivity.</p><p><strong>Abstract: </strong>RESULTS: The polycystic ovary syndrome model was successfully validated using histological, reproductive, and metabolic parameters. The epidermal thickness was significantly reduced in the polycystic ovary syndrome group compared with the control group, and the dermal collagen content was also decreased. Ki-67 immunoreactivity appeared reduced in the polycystic ovary syndrome group, suggesting decreased cellular proliferative activity. Vascular endothelial growth factor immunoreactivity showed a similar staining pattern in both groups.</p><p><strong>Abstract: </strong>CONCLUSIONS: Dehydroepiandrosterone-induced polycystic ovary syndrome resulted in significant histological alterations in the skin, including epidermal thinning, reduced proliferative activity, and decreased dermal collagen content. These findings suggest that PCOS-associated skin alterations may be linked to impaired epidermal renewal and disrupted extracellular matrix organization, supporting the concept that polycystic ovary syndrome exerts systemic effects beyond the reproductive organs.</p>","PeriodicalId":94001,"journal":{"name":"Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Association","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148893200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Missing Piece? Continuous Ketone Monitoring for Safer Sodium-Glucose Cotransporter-2 Inhibitor Use in Type 1 Diabetes.","authors":"Djordje Popovic, Dimitrios Patoulias, Theocharis Koufakis","doi":"10.1055/a-2942-0217","DOIUrl":"https://doi.org/10.1055/a-2942-0217","url":null,"abstract":"","PeriodicalId":94001,"journal":{"name":"Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Association","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148889918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of thyroid hormones on muscle and bone in mice.","authors":"Akihito Nishikawa, Naoyuki Kawao, Yuya Mizukami, Ryota Kishigami, Koji Goto, Hiroshi Kaji","doi":"10.1055/a-2937-4838","DOIUrl":"https://doi.org/10.1055/a-2937-4838","url":null,"abstract":"<p><strong>Abstract: </strong>BACKGROUND: : The significance of muscle-bone interactions in bone metabolism and the pathophysiology of osteoporosis has been recognized. However, the effects of thyroid hormones on muscle-bone interactions remain unclear.</p><p><strong>Abstract: </strong>METHODS:: We herein investigated the effects of hyperthyroidism on muscle and bone by administering L-thyroxin to male mice for 4 weeks.</p><p><strong>Abstract: </strong>RESULTS:: A micro-computed tomography analysis showed that the administration of L-thyroxin significantly decreased trabecular bone mineral density, bone volume/tissue volume, trabecular number, trabecular thickness, and connectivity density at the femurs of mice and slightly reduced cortical thickness. However, the administration of L-thyroxin did not affect muscle mass in the lower limbs, tissue weights of the soleus and gastrocnemius muscles, or grip strength. Among the myokines examined, the administration of L-thyroxin significantly decreased the messenger RNA level of follistatin in the soleus muscle and simple regression analyses showed a positive correlation between follistatin levels and bone volume/tissue volume; however, it did not affect serum follistatin levels.</p><p><strong>Abstract: </strong>CONCLUSIONS:: The present results suggest that hyperthyroidism induced by the administration of L-thyroxin caused trabecular-dominant osteopenia without reducing muscle mass or grip strength in mice. The direct effects of thyroid hormones on bone, but not muscle-bone interactions, may be crucial for hyperthyroidism-induced osteoporosis.</p>","PeriodicalId":94001,"journal":{"name":"Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Association","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148852119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cardiovascular Outcomes, Hospitalization, and Mortality following Amiodarone-Induced Thyrotoxicosis.","authors":"Irene Serrano, Pedro Iglesias, Juan J Díez","doi":"10.1055/a-2937-4553","DOIUrl":"https://doi.org/10.1055/a-2937-4553","url":null,"abstract":"<p><strong>Context: </strong>Amiodarone-induced thyrotoxicosis is a recognized adverse effect of amiodarone therapy. However, its long-term effects on cardiovascular morbidity, hospitalization, and mortality remain insufficiently characterized.</p><p><strong>Objective: </strong>The objective of this study is to evaluate the incidence of cardiovascular events, hospitalizations, and all-cause mortality following amiodarone-induced thyrotoxicosis and to assess their association with clinical, biochemical, and therapeutic parameters.</p><p><strong>Methods: </strong>A retrospective cohort study was conducted including 113 adult patients diagnosed with amiodarone-induced thyrotoxicosis and followed at a tertiary endocrinology center between 2008 and 2024. Clinical and biochemical characteristics, therapeutic interventions, cardiovascular events, hospital admissions, and mortality were recorded. The event-free survival was evaluated using Kaplan-Meier curves, and predictors of outcomes were assessed using Cox proportional hazard regression models.</p><p><strong>Results: </strong>Fifty patients (44.2%) were classified as amiodarone-induced thyrotoxicosis type 1 and 63 patients (55.8%) as amiodarone-induced thyrotoxicosis type 2. Over a median follow-up of 52 months, 42.5% experienced at least one cardiovascular event, 38.0% required hospitalization, and 33.6% died. Serum free thyroxine levels at diagnosis were significantly higher in patients who developed cardiovascular events (hazard ratio: 2.6; 95% confidence interval: 1.9-3.7 vs. hazard ratio: 2.3; 95% confidence interval: 1.6-3.4 ng/dL; <i>p</i>=0.042). Kaplan-Meier analysis showed reduced survival in older patients (<i>p</i><0.001). In the multivariate model, age at diagnosis was the only independent predictor of mortality (hazard ratio=1.06; 95% confidence interval: 1.02-1.10; <i>p</i>=0.001). Neither amiodarone-induced thyrotoxicosis type nor treatment intensity were significantly associated with cardiovascular outcomes, hospitalization, or survival.</p><p><strong>Conclusions: </strong>Amiodarone-induced thyrotoxicosis is associated with substantial long-term clinical burden. In unadjusted analysis, elevated free thyroxine levels were associated with cardiovascular events, although this association did not reach significance after multivariate adjustment. Age at amiodarone-induced thyrotoxicosis diagnosis was the only independent predictor of mortality. Amiodarone-induced thyrotoxicosis type and treatment intensity did not significantly influence outcomes.</p>","PeriodicalId":94001,"journal":{"name":"Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Association","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148820788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interpretation of 5'-Adenosine Monophosphate-Activated Protein Kinase Signaling and Translational Considerations in High-Methionine Diet Models: A Comment on the Study by Jiang et al.","authors":"Fariha Shahid Tanveer, Muhammad Hassan Saeed","doi":"10.1055/a-2937-4296","DOIUrl":"https://doi.org/10.1055/a-2937-4296","url":null,"abstract":"","PeriodicalId":94001,"journal":{"name":"Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Association","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148803541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Skeletal muscle decorin, apelin and TRIM72 gene expression in relation to insulin sensitivity.","authors":"Dominika Szutko, Marek Straczkowski","doi":"10.1055/a-2937-4910","DOIUrl":"https://doi.org/10.1055/a-2937-4910","url":null,"abstract":"<p><strong>Introduction: </strong>Skeletal muscle is an essential tissue for insulin-stimulated glucose uptake. Myokines are proteins, peptides, and other metabolites that are released by muscles and exhibit metabolic functions. Decorin, apelin and TRIM72 are myokines with connection to insulin action. The aim of this paper was to study the expression of these selected myokines in skeletal muscle in relation to insulin sensitivity in individuals without overt metabolic disturbances.</p><p><strong>Methods: </strong>Thirty healthy young male volunteers with normal glucose tolerance, 14 normal-weight, 8 with overweight and 8 with obesity, were included in the study population. The vastus lateralis muscle biopsies were performed. Insulin sensitivity (IS) was tested with hyperinsulinemic-euglycemic clamp. Muscle tissue mRNA expresion was assessed via quantitative PCR.</p><p><strong>Results: </strong>Group consisting of individuals with obesity had lower insulin sensitivity compared to those with normal weight and overweight. Apelin expression was significantly higher in the obese group compared to the normal-weight and overweight groups. All myokines' expressions correlated with insulin sensitivity, decorin - positively, apelin and TRIM72 - negatively. Only apelin was correlated positively with BMI. Apelin was correlated with insulin sensitivity independently of BMI, decorin was also correlated with insulin sensitivity independently of BMI.</p><p><strong>Conclusion: </strong>Apelin, decorin and TRIM72 are correlated with insulin sensitivity and decorin and apelin have BMI-independent associations. This suggests that these molecules can be linked to metabolic health.</p>","PeriodicalId":94001,"journal":{"name":"Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Association","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148803597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DDG [German Diabetes Society] Position Paper on Type 1 Diabetes Screening.","authors":"Oliver Blankenstein, Carolin Daniel, Andreas Fritsche, Stephan Goelz, Thomas Haak, Georg F Hoffmann, Andrea Icks, Beate Karges, Giovanni Maio, Damon Mohebbi, Andreas Neu, Matthias Schulze, Baptist Gallwitz","doi":"10.1055/a-2896-1245","DOIUrl":"10.1055/a-2896-1245","url":null,"abstract":"","PeriodicalId":94001,"journal":{"name":"Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Association","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148803577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Gallbladder Function in Patients with Type 2 Diabetes Using Three-Dimensional Ultrasound.","authors":"Tæraneh Jouleh, Ingrid Kvåle Nordaas, Spiros Kotopoulis, Georg Gjorgji Dimcevski, Erling Tjora, Sondre Vatne Meling, Odd Helge Gilja, Eirik Wigtil Søfteland","doi":"10.1055/a-2886-2833","DOIUrl":"10.1055/a-2886-2833","url":null,"abstract":"<p><strong>Aims: </strong>Symptoms related to gastrointestinal autonomic neuropathy, which can involve gallbladder, are prevalent in patients with diabetes. We hypothesize that diabetes duration may impact gallbladder volume and motility. This study aims to explore relationships between gallbladder volume parameters and diabetes duration in individuals with type 2 diabetes compared with non-diabetic controls. Autonomic neuropathy will also be explored.</p><p><strong>Methods: </strong>In this cross-sectional observational case-control study, matched individuals with longstanding type 2 diabetes, early type 2 diabetes, and non-diabetic controls were included. Gallbladder motility was investigated by gallbladder volume changes measured by three-dimensional ultrasound. Gallbladder volumes were collected at predefined time intervals before and after an intake of a standardized high-fat meal. Autonomic neuropathy was evaluated by cardiovascular (Vagus) and sudomotor (Sudoscan) testing.</p><p><strong>Results: </strong>Sixty-one adults were included in the final analysis, of which 18 adults had longstanding type 2 diabetes, 15 adults had early type 2 diabetes, and 28 adults were non-diabetic controls. Subjects with longstanding type 2 diabetes had significantly higher fasting gallbladder volume and ejection volume compared to the controls (<i>p</i>=0.020 and <i>p</i>=0.019, respectively). No other significant differences in gallbladder motility were observed. Only five participants had autonomic neuropathy.</p><p><strong>Conclusions: </strong>Specific gallbladder volumes and motility parameters were associated with diabetes duration. Associations between gallbladder motility and autonomic neuropathy could not be analyzed.</p>","PeriodicalId":94001,"journal":{"name":"Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Association","volume":" ","pages":"264-270"},"PeriodicalIF":1.7,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148159462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diabetes and Metabolic Dysfunction-Associated Steatotic Liver Disease.","authors":"Norbert Stefan, Michael Roden","doi":"10.1055/a-2766-3214","DOIUrl":"10.1055/a-2766-3214","url":null,"abstract":"","PeriodicalId":94001,"journal":{"name":"Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Association","volume":" ","pages":"239-244"},"PeriodicalIF":1.7,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147824959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}