Current protocols最新文献

{"title":"Correction: Ex Vivo Evaluation of the Function of Hematopoietic Stem Cells in Toxicology of Metals","authors":"Jiaojiao Wu,&nbsp;Ting Liu,&nbsp;Mengke Tang,&nbsp;Yalin Liu,&nbsp;Wei Wang,&nbsp;Chuanxuan Wang,&nbsp;Yingzi Ju,&nbsp;Yifan Zhao,&nbsp;Yubin Zhang","doi":"10.1002/cpz1.1115","DOIUrl":"10.1002/cpz1.1115","url":null,"abstract":"<p><i>Current Protocols</i> is issuing a correction for the following protocol article:</p><p>Wu, J., Liu, T., Tang, M., Liu, Y., Wang, W., Wang, C., Ju, Y., Zhao, Y., &amp; Zhang, Y. (2024). <i>Ex Vivo</i> evaluation of the function of hematopoietic stem cells in toxicology of metals. <i>Current Protocols</i>, <i>4</i>, e1038. doi: 10.1002/cpz1.1038</p><p>In the above-referenced article:</p><p>The status of Dr. Yifan Zhao has been corrected to co-corresponding author, and the contact email address “<span>[email protected]</span>” has been added.</p><p>The current version online now includes this correction and may be considered the authoritative version of record.</p>","PeriodicalId":93970,"journal":{"name":"Current protocols","volume":"4 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cpz1.1115","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141750079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Generation and Characterization of hiPSC-Derived Vascularized-, Perfusable Cardiac Microtissues-on-Chip","authors":"Ulgu Arslan,&nbsp;Francijna E. van den Hil,&nbsp;Christine L. Mummery,&nbsp;Valeria Orlova","doi":"10.1002/cpz1.1097","DOIUrl":"10.1002/cpz1.1097","url":null,"abstract":"<p>In the heart <i>in vivo</i>, vasculature forms a semi-permeable endothelial barrier for selective nutrient and (immune) cell delivery to the myocardium and removal of waste products. Crosstalk between the vasculature and the heart cells regulates homeostasis in health and disease. To model heart development and disease <i>in vitro</i> it is important that essential features of this crosstalk are captured. Cardiac organoid and microtissue models often integrate endothelial cells (ECs) to form microvascular networks inside the 3D structure. However, in static culture without perfusion, these networks may fail to show essential functionality. Here, we describe a protocol to generate an <i>in vitro</i> model of human induced pluripotent stem cell (hiPSC)-derived vascularized cardiac microtissues on a microfluidic organ-on-chip platform (VMToC) in which the blood vessels are perfusable. First, prevascularized cardiac microtissues (MT) are formed by combining hiPSC-derived cardiomyocytes, ECs, and cardiac fibroblasts in a pre-defined ratio. Next, these prevascularized MTs are integrated in the chips in a fibrin hydrogel containing additional vascular cells, which self-organize into tubular structures. The MTs become vascularized through anastomosis between the pre-existing microvasculature in the MT and the external vascular network. The VMToCs are then ready for downstream structural and functional assays and basic characterization. Using this protocol, cardiac MTs can be efficiently and robustly vascularized and perfused within 7 days. <i>In vitro</i> vascularized organoid and MT models have the potential to transition current 3D cardiac models to more physiologically relevant organ models that allow the role of the endothelial barrier in drug and inflammatory response to be investigated. © 2024 The Author(s). Current Protocols published by Wiley Periodicals LLC.</p><p><b>Basic Protocol</b>: Generation of VMToC</p><p><b>Support Protocol 1</b>: Functional Characterization of VMToC</p><p><b>Support Protocol 2</b>: Structural Characterization of VMToC</p>","PeriodicalId":93970,"journal":{"name":"Current protocols","volume":"4 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cpz1.1097","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141736186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis and Functionalization of Biodegradable Second Harmonic Generation Nanoprobes for Cell Targeting","authors":"Edagul Ulucay,&nbsp;Pomone Bollier,&nbsp;Marina Spilioti,&nbsp;Ali Yasin Sonay,&nbsp;Konstantinos Kalyviotis,&nbsp;Periklis Pantazis","doi":"10.1002/cpz1.1089","DOIUrl":"10.1002/cpz1.1089","url":null,"abstract":"<p>Optical imaging technologies and cell targeting have played a major role in detecting and treating diseases such as cancer. Bioharmonophores are optical imaging nanoprobes composed of biodegradable polymer–encapsulated, self-assembling triphenylalanine peptides. They produce a strong second harmonic generation (SHG) signal, a non-linear optical process in which two photons directed at a non-centrosymmetric medium combine to form a new photon with twice the energy. Bioharmonophores demonstrate superior optical properties compared to fluorescent probes and, unlike previously developed inorganic SHG nanoprobes, are both biocompatible and biodegradable. Here, we present a protocol providing five detailed procedures that describe (1) synthesis of bioharmonophores; (2) embedding and imaging of the synthesized SHG nanoprobes in polyacrylamide gel; (3) functionalization of bioharmonophores with thiol-containing polyethyleneglycol; (4) subsequent click chemistry to target cancer cells; and (5) imaging of functionalized bioharmonophores endocytosed by cancer cells using two-photon microscopy. Bioharmonophores hold great potential as clinical contrast agents due to their optical features and could be used in the future as an innovative approach to cancer treatment using targeted high-resolution optical imaging. © 2024 The Author(s). Current Protocols published by Wiley Periodicals LLC.</p><p><b>Basic Protocol 1</b>: Synthesis of bioharmonophores</p><p><b>Basic Protocol 2</b>: Imaging of bioharmonophores in polyacrylamide gel</p><p><b>Basic Protocol 3</b>: Functionalization of bioharmonophores with thiol-PEG</p><p><b>Basic Protocol 4</b>: Functionalization of thiol-PEGylated bioharmonophores with peptides</p><p><b>Basic Protocol 5</b>: Targeting of cancer cells with functionalized bioharmonophores</p>","PeriodicalId":93970,"journal":{"name":"Current protocols","volume":"4 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cpz1.1089","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141736187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expanded Antigen-Specific Elimination Assay to Measure Human CD8+ T Cell Cytolytic Potential","authors":"David R. Collins,&nbsp;Mpho J. Olatotse,&nbsp;Zachary J. Racenet,&nbsp;Umar Arshad,&nbsp;Elif Çakan,&nbsp;Gaurav D. Gaiha,&nbsp;Kiera L. Clayton,&nbsp;Bruce D. Walker","doi":"10.1002/cpz1.1109","DOIUrl":"10.1002/cpz1.1109","url":null,"abstract":"<p>Durable cellular immunity against pathogens is dependent upon a coordinated recall response to antigen by memory CD8⁺ T cells, involving their proliferation and the generation of secondary cytotoxic effector cells. Conventional assays measuring <i>ex vivo</i> cytotoxicity fail to capture this secondary cytolytic potential, especially in settings where cells have not been recently exposed to their cognate antigen <i>in vivo</i>. Here we describe the expanded antigen-specific elimination assay (EASEA), a flow cytometric endpoint assay to measure the capacity of human CD8⁺ T cells to expand <i>in vitro</i> upon antigen re-exposure and generate secondary effector cells capable of selectively eliminating autologous antigen-pulsed target cells across a range of effector-to-target ratios. Unlike alternative assays, EASEA avoids the hazards of radioactive labeling and viral infection and can be used to study responses to individual or pooled antigens of interest. © 2024 The Author(s). Current Protocols published by Wiley Periodicals LLC.</p><p><b>Basic Protocol</b>: Expanded antigen-specific elimination assay</p>","PeriodicalId":93970,"journal":{"name":"Current protocols","volume":"4 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cpz1.1109","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141636230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Describing and Sharing Molecular Visualizations Using the MolViewSpec Toolkit","authors":"Sebastian Bittrich,&nbsp;Adam Midlik,&nbsp;Mihaly Varadi,&nbsp;Sameer Velankar,&nbsp;Stephen K. Burley,&nbsp;Jasmine Y. Young,&nbsp;David Sehnal,&nbsp;Brinda Vallat","doi":"10.1002/cpz1.1099","DOIUrl":"10.1002/cpz1.1099","url":null,"abstract":"<p>With the ever-expanding toolkit of molecular viewers, the ability to visualize macromolecular structures has never been more accessible. Yet, the idiosyncratic technical intricacies across tools and the integration complexities associated with handling structure annotation data present significant barriers to seamless interoperability and steep learning curves for many users. The necessity for reproducible data visualizations is at the forefront of the current challenges. Recently, we introduced MolViewSpec (homepage: https://molstar.org/mol-view-spec/, GitHub project: https://github.com/molstar/mol-view-spec), a specification approach that defines molecular visualizations, decoupling them from the varying implementation details of different molecular viewers. Through the protocols presented herein, we demonstrate how to use MolViewSpec and its 3D view–building Python library for creating sophisticated, customized 3D views covering all standard molecular visualizations. MolViewSpec supports representations like cartoon and ball-and-stick with coloring, labeling, and applying complex transformations such as superposition to any macromolecular structure file in mmCIF, BinaryCIF, and PDB formats. These examples showcase progress towards reusability and interoperability of molecular 3D visualization in an era when handling molecular structures at scale is a timely and pressing matter in structural bioinformatics as well as research and education across the life sciences. © 2024 The Authors. Current Protocols published by Wiley Periodicals LLC.</p><p><b>Basic Protocol 1</b>: Creating a MolViewSpec view using the MolViewSpec Python package</p><p><b>Basic Protocol 2</b>: Creating a MolViewSpec view with reference to MolViewSpec annotation files</p><p><b>Basic Protocol 3</b>: Creating a MolViewSpec view with labels and other advanced features</p><p><b>Support Protocol 1</b>: Computing rotation and translation vectors</p><p><b>Support Protocol 2</b>: Creating a MolViewSpec annotation file</p>","PeriodicalId":93970,"journal":{"name":"Current protocols","volume":"4 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cpz1.1099","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141725344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole Blood Aggregometry in Mice","authors":"Siobhan Branfield,&nbsp;Yashieta Somani,&nbsp;A. Valance Washington,&nbsp;Barbara Manfredi","doi":"10.1002/cpz1.1095","DOIUrl":"10.1002/cpz1.1095","url":null,"abstract":"<p>Aggregometry plays a crucial role in both clinical diagnostics and research within hematology, serving as a fundamental tool for understanding platelet function and its implications in physiological and pathological processes. In research, aggregometry provides insights into platelet aggregation dynamics and aids in understanding the underlying mechanisms of hemostasis, thrombosis, and related disorders. Light transmission aggregometry (LTA) and lumi-aggregometry, as well as whole blood aggregometry, are commonly employed methods. While LTA and lumi-aggregometry allow for specific platelet function assessment under controlled conditions, whole blood aggregometry provides a more physiologically relevant approach by evaluating platelet aggregation within the context of whole blood.</p><p>Although both methodologies offer unique advantages, whole blood aggregometry allows for preservation of the native cellular environment, simplicity, and potential for better clinical correlation. In a clinical setting, with human blood samples, protocols are established for both LTA and whole blood aggregometry as they are frequently used diagnostic tools. A protocol for LTA and lumi-aggregometry in murine models has been described; however, to date, there is no standardized protocol for whole blood aggregometry in murine models accessible to hematology researchers. This article aims to outline a simple, basic protocol for murine whole blood aggregometry, offering an alternative method to the commonly used LTA aggregometry in research settings. Standardizing whole blood aggregometry protocols in murine models could enhance experimental reliability and facilitate translational research efforts in hematology. © 2024 Wiley Periodicals LLC.</p><p><b>Basic Protocol 1</b>: Whole blood aggregometry in mice</p><p><b>Support Protocol</b>: Phenylhydrazine-induced anemia in wild-type mice</p><p><b>Basic Protocol 2</b>: Hematocrit percentage in mice</p>","PeriodicalId":93970,"journal":{"name":"Current protocols","volume":"4 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141629586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Understanding Human Health Impacts Following Microplastic Exposure Necessitates Standardized Protocols","authors":"Sarah E. Morgan,&nbsp;Samantha S Romanick,&nbsp;Lisa DeLouise,&nbsp;James McGrath,&nbsp;Alison Elder","doi":"10.1002/cpz1.1104","DOIUrl":"10.1002/cpz1.1104","url":null,"abstract":"<p>Microplastics (MPs; 1 µm to 5 mm) are a persistent and pervasive environmental pollutant of emergent and increasing concern. Human exposure to MPs through food, water, and air has been documented and thus motivates the need for a better understanding of the biological implications of MP exposure. These impacts are dependent on the properties of MPs, including size, morphology, and chemistry, as well as the dose and route of exposure. This overview offers a perspective on the current methods used to assess the bioactivity of MPs. First, we discuss methods associated with MP bioactivity research with an emphasis on the variety of assays, exposure conditions, and reference MP particles that have been used. Next, we review the challenges presented by common instrumentation and laboratory materials, the lack of standardized reference materials, and the limited understanding of MP dosimetry. Finally, we propose solutions that can help increase the applicability and impact of future studies while reducing redundancy in the field. The excellent protocols published in this issue are intended to contribute toward standardizing the field so that the MP knowledge base grows from a reliable foundation. © 2024 Wiley Periodicals LLC.</p>","PeriodicalId":93970,"journal":{"name":"Current protocols","volume":"4 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141629585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Update: Induction of Inflammatory Bowel Disease in Immunodeficient Mice by Injection of Naïve CD4+ T cells (T Cell Transfer Model of Colitis)","authors":"Claire F. Pearson,&nbsp;Kevin J. Maloy","doi":"10.1002/cpz1.1092","DOIUrl":"10.1002/cpz1.1092","url":null,"abstract":"<p>The intestinal inflammation induced by injection of naïve CD4⁺ T cells into lymphocyte-deficient hosts (more commonly known as the T cell transfer model of colitis) shares many features of idiopathic inflammatory bowel disease (IBD) in humans, such as epithelial cell hyperplasia, crypt abscess formation, and dense lamina propria lymphocyte infiltration. As such, it provides a useful tool for studying mucosal immune regulation as it relates to the pathogenesis and treatment of IBD in humans. In the IBD model described here, colitis is induced in <i>Rag</i> (recombination-activating gene)-deficient mice by reconstitution of these mice with naïve CD4⁺CD45RB^hi T cells through adoptive T cell transfer. Although different recipient hosts of cell transfer can be used, Rag-deficient mice are the best characterized and support studies that are both flexible and reproduceable. As described in the Basic Protocol, in most studies the transferred cells consist of naïve CD4⁺ T cells (CD45RB^hi T cells) derived by fluorescence-activated cell sorting from total CD4⁺ T cells previously purified using immunomagnetic negative selection beads. In a Support Protocol, methods to characterize colonic disease progression are described, including the monitoring of weight loss and diarrhea and the histological assessment of colon pathology. © 2024 The Author(s). Current Protocols published by Wiley Periodicals LLC.</p><p><b>Basic Protocol</b>: Induction of IBD in Rag-deficient mice by the transfer of naïve CD4⁺CD45RB^hi T cells</p><p><b>Support Protocol</b>: Monitoring development of colitis</p>","PeriodicalId":93970,"journal":{"name":"Current protocols","volume":"4 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cpz1.1092","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141617849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protocol for the Growth and Maturation of hiPSC-Derived Kidney Organoids using Mechanically Defined Hydrogels","authors":"Ivan Krupa,&nbsp;Niall J. Treacy,&nbsp;Shane Clerkin,&nbsp;Jessica L. Davis,&nbsp;Aline F. Miller,&nbsp;Alberto Saiani,&nbsp;Jacek K. Wychowaniec,&nbsp;Emmanuel G. Reynaud,&nbsp;Dermot F. Brougham,&nbsp;John Crean","doi":"10.1002/cpz1.1096","DOIUrl":"10.1002/cpz1.1096","url":null,"abstract":"<p>With recent advances in the reprogramming of somatic cells into induced Pluripotent Stem Cells (iPSCs), gene editing technologies, and protocols for the directed differentiation of stem cells into heterogeneous tissues, iPSC-derived kidney organoids have emerged as a useful means to study processes of renal development and disease. Considerable advances guided by knowledge of fundamental renal developmental signaling pathways have been made with the use of exogenous morphogens to generate more robust kidney-like tissues <i>in vitro</i>. However, both biochemical and biophysical microenvironmental cues are major influences on tissue development and self-organization. In the context of engineering the biophysical aspects of the microenvironment, the use of hydrogel extracellular scaffolds for organoid studies has been gaining interest. Two families of hydrogels have recently been the subject of significant attention: self-assembling peptide hydrogels (SAPHs), which are fully synthetic and chemically defined, and gelatin methacryloyl (GelMA) hydrogels, which are semi-synthetic. Both can be used as support matrices for growing kidney organoids. Based on our recently published work, we highlight methods describing the generation of human iPSC (hiPSC)-derived kidney organoids and their maturation within SAPHs and GelMA hydrogels. We also detail protocols required for the characterization of such organoids using immunofluorescence imaging. Together, these protocols should enable the user to grow hiPSC-derived kidney organoids within hydrogels of this kind and evaluate the effects that the biophysical microenvironment provided by the hydrogels has on kidney organoid maturation. © 2024 The Authors. Current Protocols published by Wiley Periodicals LLC.</p><p><b>Basic Protocol 1</b>: Directed differentiation of human induced pluripotent stem cells (hiPSCs) into kidney organoids and maturation within mechanically tunable self-assembling peptide hydrogels (SAPHs)</p><p><b>Alternate Protocol</b>: Encapsulation of day 9 nephron progenitor aggregates in gelatin methacryloyl (GelMA) hydrogels.</p><p><b>Support Protocol 1</b>: Human induced pluripotent stem cell (hiPSC) culture.</p><p><b>Support Protocol 2</b>: Organoid fixation with paraformaldehyde (PFA)</p><p><b>Basic Protocol 2</b>: Whole-mount immunofluorescence imaging of kidney organoids.</p><p><b>Basic Protocol 3</b>: Immunofluorescence of organoid cryosections</p>","PeriodicalId":93970,"journal":{"name":"Current protocols","volume":"4 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cpz1.1096","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141565395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Mouse Models of Sepsis","authors":"Shravan Kumar Kannan,&nbsp;Caleb Y. Kim,&nbsp;Mohammad Heidarian,&nbsp;Roger R. Berton,&nbsp;Isaac J. Jensen,&nbsp;Thomas S. Griffith,&nbsp;Vladimir P. Badovinac","doi":"10.1002/cpz1.1111","DOIUrl":"10.1002/cpz1.1111","url":null,"abstract":"<p><i>Current Protocols</i> is issuing corrections for the following protocol article.</p><p>Kannan, S. K., Kim, C. Y., Heidarian, M., Berton, R. R., Jensen, I. J., Griffith, T. S., &amp; Badovinac, V. P. (2024). Mouse models of sepsis. <i>Current Protocols</i>, <i>4</i>, e997. doi: 10.1002/cpz1.997</p><p>In the above-referenced article:</p><p>A sentence has been added to the Acknowledgements to thank Dr. Patricia De Assis for her contribution to this article.</p><p>The current version online now includes these corrections and may be considered the authoritative version of record.</p>","PeriodicalId":93970,"journal":{"name":"Current protocols","volume":"4 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cpz1.1111","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141565394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}