用于病毒检测的反转录酶环路介导等温扩增（RT-LAMP）--案例研究

Current protocols Pub Date : 2025-04-21 DOI:10.1002/cpz1.70127

Ashley Hassman, Renée Richie Casey, Colby Rouchka, Diego Sunino, Felix Veloz Espinal, Mona Youssef

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Reverse-Transcriptase Loop-Mediated Isothermal Amplification (RT-LAMP) for Viral Detection — A Case Study

An assay and protocol framework are provided for utilizing reverse-transcriptase loop-mediated isothermal amplification (RT-LAMP) at the point-of-care for diagnosing and monitoring a hypothetical zoonotic viral outbreak in a resource-limited area. This manuscript utilizes a previously published decision tree algorithm to determine an appropriate molecular diagnostic point-of-care test that can effectively address the outbreak presented in the hypothetical case study. © 2025 Wiley Periodicals LLC.

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Current protocols

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4.00

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0.00%

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