Sara G. Dosil, Francisco Sánchez-Madrid, Lola Fernández-Messina
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引用次数: 0
Abstract
Natural killer (NK) cells are critical components of the immune system, recognized for their ability to identify and eliminate target cells undergoing various forms of stress. Beyond their direct cytotoxic activity, NK cells secrete extracellular vesicles (EVs), which serve as mediators of intercellular communication. These vesicles play an essential role in modulating immune responses and influencing tumoral and post-infection microenvironments. In this study, we present a detailed methodology for the isolation and expansion of human primary NK cells, the accumulation of their EVs, and the subsequent procedures for their isolation and characterization. © 2025 Wiley Periodicals LLC.
Basic Protocol 1: NK cell isolation and expansion
Basic Protocol 2: NK-EV purification using ultracentrifugation
Alternate Protocol: NK-EV isolation using size exclusion chromatography
Basic Protocol 3: Characterization of NK-EVs
天然杀伤细胞胞外囊泡的分离与表征
自然杀伤细胞（NK）是免疫系统的重要组成部分，因其识别和消除遭受各种形式压力的靶细胞的能力而得到认可。除了直接的细胞毒性活性外，NK细胞还分泌细胞外囊泡（EVs），作为细胞间通讯的介质。这些囊泡在调节免疫反应和影响肿瘤和感染后微环境中发挥重要作用。在这项研究中，我们提出了一种详细的方法来分离和扩增人类原代NK细胞，积累它们的ev，以及随后的分离和表征程序。©2025 Wiley期刊有限公司基本方案1:NK细胞分离和扩增基本方案2：用超离心纯化NK- ev备用方案：用大小排斥色谱分离NK- ev基本方案3:NK- ev的表征
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