Current opinion in toxicology最新文献_第3页

Recent advances of single-cell RNA sequencing in toxicology research: Insight into hepatotoxicity and nephrotoxicity 单细胞 RNA 测序在毒理学研究中的最新进展：深入了解肝毒性和肾毒性

IF 4.6

Current opinion in toxicology Pub Date : 2024-01-16 DOI: 10.1016/j.cotox.2024.100462

Junhui Chen , Jiangpeng Wu , Yunmeng Bai , Chuanbin Yang , Jigang Wang

{"title":"Recent advances of single-cell RNA sequencing in toxicology research: Insight into hepatotoxicity and nephrotoxicity","authors":"Junhui Chen , Jiangpeng Wu , Yunmeng Bai , Chuanbin Yang , Jigang Wang","doi":"10.1016/j.cotox.2024.100462","DOIUrl":"10.1016/j.cotox.2024.100462","url":null,"abstract":"<div><p><span>The advanced single-cell RNA sequencing (scRNA-seq) technology enables the measurement of </span>gene expression levels<span> and identification of cell-specific markers in individual cells, and the revealing of cell types, providing valuable insights into cell behavior. ScRNA-seq has emerged as an effective method for detecting rare cell populations, cellular heterogeneity, and cell-to-cell crosstalk under physiology and pathology conditions, thereby advancing the revolution of toxicology research. In this review, we first briefly introduce the concept of hepatotoxicity and nephrotoxicity, along with their underlying mechanisms. We then summarize the single-cell sequencing technology, with an emphasis on single-cell isolation techniques, particularly for the most used droplet-based methods. Finally, we review the recent advances in the application of single-cell RNA sequencing in studying hepatotoxicity and nephrotoxicity. Overall, this review provides a comprehensive understanding of the recent advances in hepatotoxicity and nephrotoxicity studies at a single-cell resolution.</span></p></div>","PeriodicalId":93968,"journal":{"name":"Current opinion in toxicology","volume":"37 ","pages":"Article 100462"},"PeriodicalIF":4.6,"publicationDate":"2024-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139474840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancing immunotoxicology with single-cell sequencing: Challenges and progress defining mechanisms of arsenic toxicity 利用单细胞测序推进免疫毒理学研究：挑战与进步定义砷的毒性机制

IF 4.6

Current opinion in toxicology Pub Date : 2024-01-14 DOI: 10.1016/j.cotox.2024.100461

Britton C. Goodale

{"title":"Advancing immunotoxicology with single-cell sequencing: Challenges and progress defining mechanisms of arsenic toxicity","authors":"Britton C. Goodale","doi":"10.1016/j.cotox.2024.100461","DOIUrl":"10.1016/j.cotox.2024.100461","url":null,"abstract":"<div><p><span><span>Defining mechanisms of immunotoxicity is complicated by the many cell types that comprise the immune system, their </span>phenotypic heterogeneity, distribution in tissues throughout the body, and complexity of </span><em>in vivo</em><span> interactions. Single-cell RNA-sequencing (scRNA-seq) methods hold promise for determining how chemical exposures alter gene expression and phenotype of individual immune cell<span> phenotypes, leading to adverse effects on immune function. Using arsenic as a case study, this review will examine challenges in defining mechanisms of immunotoxicity and highlight findings from recent studies that have addressed immunotoxicological questions with scRNA-seq. Advancements in immunotherapeutic development driven by single-cell sequencing technologies will be discussed, along with how these state-of-the art methods may be applied to accelerate immunotoxicity testing in future studies. We will finally consider how cell-type-specific gene expression data can be leveraged to glean immune profiles from existing gene expression data, improving our understanding of immunotoxicity and ability to assess the health impacts of immunotoxic chemicals.</span></span></p></div>","PeriodicalId":93968,"journal":{"name":"Current opinion in toxicology","volume":"37 ","pages":"Article 100461"},"PeriodicalIF":4.6,"publicationDate":"2024-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139458681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single cell approaches to understand environmental impacts on aggressive breast cancers 通过单细胞方法了解环境对侵袭性乳腺癌的影响

IF 4.6

Current opinion in toxicology Pub Date : 2024-01-10 DOI: 10.1016/j.cotox.2024.100459

David Aguilar Jr. , Justin A. Colacino

引用次数: 0

Application of single cell gene expression technologies to neurotoxicology 单细胞基因表达技术在神经毒理学中的应用

IF 4.6

Current opinion in toxicology Pub Date : 2023-12-27 DOI: 10.1016/j.cotox.2023.100458

Anke M. Tukker, Aaron B. Bowman

引用次数: 0

Editorial: Guardians of tomorrow: Developmental toxicology for future generations 社论：明日守护者：面向未来一代的发育毒理学

IF 4.6

Current opinion in toxicology Pub Date : 2023-12-22 DOI: 10.1016/j.cotox.2023.100457

Emanuela Corsini

引用次数: 0

Transcriptomic-based roadmap to the healthy and ozone-exposed lung 基于转录组学的健康和臭氧暴露肺路线图

IF 4.6

Current opinion in toxicology Pub Date : 2023-11-28 DOI: 10.1016/j.cotox.2023.100445

Philip Moos, Jenna Cheminant, Ujjwal Adhikari, Alessandro Venosa

{"title":"Transcriptomic-based roadmap to the healthy and ozone-exposed lung","authors":"Philip Moos, Jenna Cheminant, Ujjwal Adhikari, Alessandro Venosa","doi":"10.1016/j.cotox.2023.100445","DOIUrl":"10.1016/j.cotox.2023.100445","url":null,"abstract":"<div><p>The lung is constantly exposed to a myriad of exogenous stressors. Ground-level ozone represents a ubiquitous and extremely reactive anthropogenic toxicant, impacting the health of millions across the globe. While abundant, epidemiological, <em>in vivo</em>, and <em>in vitro</em> data focuses the ozone toxicity in individual cell types (e.g. epithelial type II, alveolar macrophages) or signaling pathways involved in the injury (e.g. akt, glutathione). When appropriately used, bulk and single-cell RNA sequencing techniques have the potential to provide complete, and in certain cases unbiased, information of the molecular events taking place in the steady-state and injured lung, and even capture the phenotypic diversity of neighboring cells. To this end, this review compiles information pertaining to the latest understanding of lung cell identity and activation in the steady-state and ozone-exposed lung. In addition, it discusses the value and benefits of multi-omics approaches and other tools developed to predict cell–cell communication and dissect spatial heterogeneity.</p></div>","PeriodicalId":93968,"journal":{"name":"Current opinion in toxicology","volume":"37 ","pages":"Article 100445"},"PeriodicalIF":4.6,"publicationDate":"2023-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2468202023000608/pdfft?md5=c638bcf1fa63e7c44f50b43e2c986646&pid=1-s2.0-S2468202023000608-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138529934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advanced technologies in genomic toxicology: Current trend and future directions 基因组毒理学的先进技术:当前趋势和未来方向

IF 4.6

Current opinion in toxicology Pub Date : 2023-11-21 DOI: 10.1016/j.cotox.2023.100444

Pasquale Capone, Pieranna Chiarella, Renata Sisto

{"title":"Advanced technologies in genomic toxicology: Current trend and future directions","authors":"Pasquale Capone, Pieranna Chiarella, Renata Sisto","doi":"10.1016/j.cotox.2023.100444","DOIUrl":"10.1016/j.cotox.2023.100444","url":null,"abstract":"<div><p>Toxicogenomics is a subdiscipline of toxicology that assesses the toxicity using a combination of genomics, advanced computational technologies, and other high-throughput techniques. In the last few years, the rapid evolution of technologies such as transcriptomics, proteomics, metabolomics, and computational technologies allowed the application of a more flexible strategy for the risk assessment due to exposure to chemical and xenobiotic substances. The integration of bioinformatics approaches with novel genomic technologies permits extraordinary advancements in different fields of biology, toxicology, medicine, and, in particular, in occupational medicine, making accessible a “personalized” approach to this discipline. The scope of this paper is to investigate the recent trend of genomic toxicology in combination with bioinformatics approaches and its novel application in toxicological studies with reference to our experience and findings. In particular, it is shown how the dose biomarkers, the pollutants urine metabolites in our case, allow a precise exposure assessment whilst effect biomarkers can be individuated, significantly associated with the former. Among the effect biomarkers, our experience is mainly focused on oxidative stress biomarkers and miRNA profiles. Finally, it is shown how the early effect biomarkers are dysregulated when specific clinical outcomes are occurring. This early dysregulation can be used as individual susceptibility biomarker.</p></div>","PeriodicalId":93968,"journal":{"name":"Current opinion in toxicology","volume":"37 ","pages":"Article 100444"},"PeriodicalIF":4.6,"publicationDate":"2023-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2468202023000591/pdfft?md5=9126629f8ba9407328b06c7c140847e8&pid=1-s2.0-S2468202023000591-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138529967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0